##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935428.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312734
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.830843464413846	33.0	31.0	34.0	30.0	34.0
2	31.89364124143841	33.0	31.0	34.0	30.0	34.0
3	31.98929121873541	33.0	31.0	34.0	30.0	34.0
4	35.70422787416782	37.0	35.0	37.0	33.0	37.0
5	35.712321014024695	37.0	35.0	37.0	35.0	37.0
6	35.65721347854726	37.0	35.0	37.0	33.0	37.0
7	35.917210792558535	37.0	35.0	37.0	35.0	37.0
8	35.699325944732585	37.0	35.0	37.0	35.0	37.0
9	37.550634085197004	39.0	37.0	39.0	35.0	39.0
10	37.018872268445385	39.0	37.0	39.0	33.0	39.0
11	36.897737374254156	39.0	37.0	39.0	33.0	39.0
12	36.97072911803642	39.0	37.0	39.0	33.0	39.0
13	36.94657120747984	39.0	37.0	39.0	33.0	39.0
14	38.08913645462278	40.0	37.0	41.0	33.0	41.0
15	38.12172005602205	40.0	37.0	41.0	33.0	41.0
16	38.13934206066497	40.0	37.0	41.0	33.0	41.0
17	38.05390203815383	40.0	37.0	41.0	33.0	41.0
18	37.95717446775854	39.0	37.0	41.0	33.0	41.0
19	37.79466255667756	39.0	37.0	41.0	33.0	41.0
20	37.71745317106551	39.0	36.0	41.0	33.0	41.0
21	37.76732942372751	39.0	36.0	41.0	33.0	41.0
22	37.8670115817276	40.0	36.0	41.0	33.0	41.0
23	37.83022952413233	40.0	36.0	41.0	33.0	41.0
24	37.84505042624083	40.0	36.0	41.0	33.0	41.0
25	37.63752262305985	39.0	36.0	41.0	33.0	41.0
26	37.63090677700538	39.0	36.0	41.0	33.0	41.0
27	37.629985866583105	40.0	36.0	41.0	33.0	41.0
28	37.53698030914451	40.0	36.0	41.0	33.0	41.0
29	37.506519917885484	40.0	36.0	41.0	33.0	41.0
30	37.41025600030697	39.0	36.0	41.0	33.0	41.0
31	37.38946836608747	40.0	36.0	41.0	33.0	41.0
32	37.247648161057	40.0	36.0	41.0	32.0	41.0
33	37.06920258110727	40.0	35.0	41.0	32.0	41.0
34	36.935545863257595	40.0	35.0	41.0	31.0	41.0
35	36.86402821567211	40.0	35.0	41.0	31.0	41.0
36	36.80536174512525	40.0	35.0	41.0	31.0	41.0
37	36.72817794035826	40.0	35.0	41.0	31.0	41.0
38	36.64025337827035	40.0	35.0	41.0	30.0	41.0
39	36.540168321960515	40.0	35.0	41.0	30.0	41.0
40	36.41917412241713	39.0	35.0	41.0	30.0	41.0
41	36.267441339924666	39.0	35.0	41.0	30.0	41.0
42	36.28904436358055	39.0	35.0	41.0	30.0	41.0
43	36.30026156414077	39.0	35.0	41.0	30.0	41.0
44	36.13177332813189	39.0	35.0	41.0	29.0	41.0
45	36.12338920616243	39.0	35.0	41.0	29.0	41.0
46	35.97036139338863	39.0	35.0	41.0	28.0	41.0
47	35.846198366663046	39.0	35.0	41.0	28.0	41.0
48	35.76235394936272	39.0	35.0	41.0	27.0	41.0
49	35.72607391585181	39.0	35.0	40.0	27.0	41.0
50	35.604302698139634	39.0	35.0	40.0	27.0	41.0
51	34.32728772695006	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	4.0
11	2.0
12	3.0
13	6.0
14	5.0
15	7.0
16	21.0
17	44.0
18	92.0
19	208.0
20	370.0
21	721.0
22	1046.0
23	1300.0
24	1738.0
25	2462.0
26	3356.0
27	4076.0
28	4277.0
29	4496.0
30	5114.0
31	6427.0
32	8159.0
33	10988.0
34	17795.0
35	22973.0
36	23396.0
37	33364.0
38	62462.0
39	97768.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.007264959997954	20.82760428990772	23.8880326411583	13.277098108936029
2	31.147876470099188	30.192112146424755	22.874711416091632	15.785299967384422
3	28.264915231474674	29.014114231263633	28.345814653987095	14.375155883274605
4	25.142453330945784	24.40924235932134	34.08423772279317	16.3640665869397
5	28.21791042867101	28.036286428722175	25.966796063107942	17.77900707949887
6	23.741902063734678	37.664276989390345	25.139575485876176	13.454245460998804
7	74.21195009177129	5.904378801153696	15.559868770264826	4.323802336810196
8	75.4161683731222	9.643339067706101	10.65250340545	4.287989153721694
9	70.06497534646057	7.866429617502415	11.644400672776225	10.42419436326079
10	36.78621448259543	33.76415739893967	17.668369924600462	11.781258193864435
11	26.717274105150064	24.302442331182412	31.025408174359043	17.954875389308487
12	23.943351218607507	21.839006951594648	33.30402194836506	20.913619881432783
13	22.67390178234538	23.42150197931789	36.116316102502445	17.788280135834285
14	18.434196473680508	30.076998343640284	30.994711160283178	20.49409402239603
15	16.311625854560106	27.545134203508415	36.78941208822833	19.353827853703148
16	19.193627811494753	26.551638133365735	32.54906725843688	21.70566679670263
17	19.366938036798047	27.43609585142645	31.2195028362762	21.977463275499307
18	19.38708295228533	27.850825302013853	31.147556709535902	21.61453503616492
19	20.625835374471595	28.44813803423996	29.281434062174245	21.6445925291142
20	23.082555782230266	26.9283160769216	31.756061061477165	18.23306707937097
21	22.03054352900548	29.374164625528405	30.404752921012744	18.19053892445337
22	20.028522642245488	25.005276049294288	31.39377234326936	23.572428965190863
23	19.9562567549419	29.537882033932988	30.845382977226656	19.660478233898456
24	21.035448656046352	26.631258513625	30.131997160526197	22.20129566980245
25	19.98631424789118	31.421271751712315	28.135731963905425	20.456682036491074
26	19.247667346690797	28.377151189189536	30.381090639329273	21.994090824790398
27	21.98513752901827	29.034578907314202	28.622087780669837	20.35819578299769
28	18.196294614592592	29.747964724014658	32.18102285008985	19.874717811302897
29	20.91809652931885	27.936521132975628	30.246151681620802	20.899230656084725
30	21.35233137426695	28.604820710252167	30.14638638587426	19.89646152960663
31	22.349344810605817	28.287298470904986	27.350400020464676	22.012956698024517
32	22.81107906399688	29.9065659634066	28.547263808859924	18.735091163736595
33	21.47352062775394	26.896979541719162	27.267262274009223	24.36223755651768
34	21.743398543170876	28.48970690746769	28.453254203252605	21.313640346108834
35	20.87684741665441	27.665683935868824	28.151080470943356	23.306388176533414
36	20.7857156561167	30.747536244859848	26.067200879981073	22.399547219042383
37	21.511891895348764	28.767578836966877	29.969558794374773	19.750970473309586
38	18.99377745943837	29.848049780324494	26.95869333043417	24.199479429802963
39	22.066676472657274	26.063363753221587	29.506545498730553	22.363414275390586
40	20.794029430762244	26.99130890788977	30.14606662531097	22.068595036037014
41	20.26482569851695	27.827802541456958	28.280903259639185	23.62646850038691
42	19.687018360651546	29.65779224516682	29.15768672418093	21.497502670000703
43	21.32291340244425	27.135201161370365	26.828550781175053	24.71333465501033
44	21.811507543151688	26.55899262632141	29.53820179449628	22.091298036030622
45	20.943037853255483	25.872466696937334	28.52136320323342	24.663132246573767
46	23.085433627299878	28.160993048405352	26.856689710744597	21.896883613550173
47	19.796696233860086	26.22036618979708	32.27503245569717	21.70790512064566
48	20.55261020547814	26.918083738896314	29.031061541118007	23.498244514507537
49	18.69608037501519	25.73049300683648	32.257125864152925	23.31630075399541
50	19.6198686423606	25.165156330939393	30.38428824496217	24.830686781737835
51	19.224644586133902	25.044286838015694	29.41157661143336	26.319491964417047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1734.0
1	1268.5
2	803.0
3	2871.0
4	4939.0
5	3189.5
6	1440.0
7	1417.5
8	1395.0
9	1361.5
10	1328.0
11	1314.5
12	1301.0
13	1254.0
14	1207.0
15	1170.0
16	1133.0
17	1086.5
18	1040.0
19	1077.0
20	1114.0
21	1188.5
22	1263.0
23	1402.5
24	1542.0
25	1885.0
26	2739.0
27	3250.0
28	3937.0
29	4624.0
30	5457.0
31	6290.0
32	7343.0
33	8396.0
34	9128.0
35	9860.0
36	10747.0
37	11634.0
38	12253.5
39	12873.0
40	14194.0
41	15515.0
42	16904.0
43	18293.0
44	20019.0
45	21745.0
46	31064.0
47	40383.0
48	34843.0
49	29303.0
50	28038.0
51	26773.0
52	22770.0
53	18767.0
54	16357.0
55	13947.0
56	12850.5
57	11754.0
58	11024.0
59	10294.0
60	9981.5
61	9669.0
62	8344.5
63	7020.0
64	5774.5
65	4529.0
66	3458.5
67	2388.0
68	1796.5
69	1205.0
70	977.5
71	750.0
72	615.0
73	480.0
74	384.5
75	205.0
76	121.0
77	100.5
78	80.0
79	52.0
80	24.0
81	16.0
82	8.0
83	4.0
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	312734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.206427980789364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.36086192894516	23.69639322290571
2	8.80527986498388	5.847837833745639
3	3.5553592446991114	3.5418234211480417
4	2.2949842313213598	3.048329143776799
5	1.7312748037771255	2.874472604329017
6	1.4516653057212732	2.8922771655986423
7	1.21603879453194	2.826621325672954
8	1.1281798251450401	2.997025769044665
9	1.0343311576824556	3.091179878628205
>10	7.324182707804078	38.542278399741946
>50	0.05491140251401846	1.252849487791156
>100	0.028953284961937005	1.7753397743110984
>500	0.00798711309294814	1.8174440042527433
>1k	0.0059903348197111045	5.796127969053395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	4033	1.28959435174941	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG	3973	1.2704087179519974	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC	3770	1.2054973236040851	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3131	1.001170323661642	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1467	0.4690887463467356	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1109	0.354614464688841	TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	947	0.30281325343582727	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	896	0.2865054647080266	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT	767	0.24525635204358975	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT	662	0.21168149289811788	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	580	0.18546112670832082	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA	576	0.18418208445516	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	528	0.16883357741722999	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC	526	0.16819405629064954	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	430	0.13749704221478956	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT	427	0.13653776052491895	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC	315	0.10072457743641561	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.197605632902083E-4	0.0	0.0	0.15604315488562165	0.0
2	3.197605632902083E-4	0.0	0.0	0.8591966335607897	0.0
3	3.197605632902083E-4	0.0	0.0	1.3836039573567314	0.0
4	3.197605632902083E-4	0.0	0.0	2.161261647278518	0.0
5	3.197605632902083E-4	0.0	0.0	4.259210703025574	0.0
6	3.197605632902083E-4	0.0	0.0	5.119046857712944	0.0
7	3.197605632902083E-4	0.0	0.0	5.993272237748374	0.0
8	3.197605632902083E-4	0.0	0.0	6.9647048290240265	0.0
9	3.197605632902083E-4	0.0	0.0	7.30748815287113	0.0
10	3.197605632902083E-4	0.0	0.0	9.253231180492048	0.0
11	3.197605632902083E-4	0.0	0.0	10.126177518274316	0.0
12	6.395211265804166E-4	0.0	0.0	12.23499843317324	0.0
13	6.395211265804166E-4	0.0	0.0	12.644291954184707	0.0
14	6.395211265804166E-4	0.0	0.0	12.84989799638031	0.0
15	6.395211265804166E-4	0.0	0.0	13.255993911758875	0.0
16	6.395211265804166E-4	0.0	0.0	13.700141334168974	0.0
17	6.395211265804166E-4	0.0	0.0	14.234461235426913	0.0
18	6.395211265804166E-4	0.0	0.0	14.824739235260637	0.0
19	6.395211265804166E-4	0.0	0.0	15.450190897056284	0.0
20	6.395211265804166E-4	0.0	0.0	15.852129925112076	0.0
21	6.395211265804166E-4	0.0	0.0	16.2857252489336	0.0
22	6.395211265804166E-4	0.0	0.0	16.81045233329283	0.0
23	6.395211265804166E-4	0.0	0.0	17.292971023297756	0.0
24	9.592816898706249E-4	0.0	0.0	17.6670908823473	0.0
25	9.592816898706249E-4	0.0	0.0	18.002519713238726	0.0
26	9.592816898706249E-4	0.0	0.0	18.347861121592153	0.0
27	9.592816898706249E-4	0.0	0.0	18.702795346844283	0.0
28	9.592816898706249E-4	0.0	0.0	19.031509205906616	0.0
29	9.592816898706249E-4	0.0	0.0	19.417779966361188	0.0
30	9.592816898706249E-4	0.0	0.0	19.840183670467553	0.0
31	9.592816898706249E-4	0.0	0.0	20.243401740776505	0.0
32	9.592816898706249E-4	0.0	0.0	20.59993476884509	0.0
33	9.592816898706249E-4	0.0	0.0	20.95518875466051	0.0
34	9.592816898706249E-4	0.0	0.0	21.325151726387283	0.0
35	9.592816898706249E-4	0.0	0.0	21.70534703613934	0.0
36	9.592816898706249E-4	0.0	0.0	22.100890852929325	0.0
37	0.0012790422531608332	0.0	0.0	22.497074190845893	0.0
38	0.0012790422531608332	0.0	0.0	22.862880275249893	0.0
39	0.0012790422531608332	0.0	0.0	23.23028516247034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	25	3.8842725E-5	45.0	2
TAACGCG	20	7.025402E-4	45.0	1
TATTGCG	20	7.025402E-4	45.0	1
CGGTCTA	25	3.8842725E-5	45.0	31
ACGTAGG	45	3.8198777E-10	45.0	2
GCTACGA	35	1.2081364E-7	45.0	10
ATACGAG	25	3.8842725E-5	45.0	1
TATCGCG	20	7.025402E-4	45.0	1
TAATGCG	20	7.025402E-4	45.0	1
GTTATCG	20	7.025402E-4	45.0	45
CTAGCGG	35	1.2081364E-7	45.0	2
CGGGTTG	30	2.1604155E-6	44.999996	6
CGGCGAA	30	2.1604155E-6	44.999996	31
TAGATCG	30	2.1604155E-6	44.999996	1
CGTTTTT	1480	0.0	43.47973	1
GGCGACT	75	0.0	42.0	10
CGACTGG	55	6.002665E-11	40.909092	2
ACGGCTG	540	0.0	40.416664	8
TACGGCT	545	0.0	40.045868	7
CAGTGCG	45	1.920671E-8	40.0	1
>>END_MODULE