SRR2935426.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935426.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325347
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	14753	4.534543118578011	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	14540	4.469074557318802	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	13727	4.219187513639284	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3084	0.9479109996403839	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	2866	0.8809056176943386	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	2786	0.8563164867049642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.8538575736060268	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2470	0.7591894192969353	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	2269	0.6974092276861321	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	2193	0.6740495532462264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	2128	0.6540708843173596	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	1409	0.433076069550357	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	1184	0.36391913864274145	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	825	0.2535754133279237	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	745	0.2289862823385493	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATG	707	0.21730644511859645	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	639	0.1964056837776282	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTAT	562	0.1727386452003553	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGT	518	0.1592146231561994	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	448	0.13769913354049676	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAGCTA	402	0.12356038322160648	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	402	0.12356038322160648	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	377	0.11587627978742697	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	359	0.11034372531481772	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	327	0.10050807291906794	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGG	30	2.1606866E-6	45.000004	31
ACGGCTA	55	1.8189894E-12	45.000004	30
CGGCTAT	55	1.8189894E-12	45.000004	31
TTTTGCG	30	2.1606866E-6	45.000004	1
TGGTATG	30	2.1606866E-6	45.000004	1
ATTTACG	20	7.0258224E-4	45.0	1
GTGCACG	25	3.8846203E-5	45.0	1
CGGAATT	20	7.0258224E-4	45.0	13
TGCTTCG	20	7.0258224E-4	45.0	35
CGACGTT	25	3.8846203E-5	45.0	27
TTTACGG	20	7.0258224E-4	45.0	2
CACGGCC	20	7.0258224E-4	45.0	31
GCGAAGG	50	2.1827873E-11	45.0	2
TCGGGTG	20	7.0258224E-4	45.0	5
CCGCTTC	20	7.0258224E-4	45.0	20
GGATAGT	45	3.8380676E-10	45.0	8
TTCCCGT	20	7.0258224E-4	45.0	24
TCGGAAT	20	7.0258224E-4	45.0	12
GCTTCGC	20	7.0258224E-4	45.0	36
TTGCGAG	20	7.0258224E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content