##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935426.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325347
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520425883748736	31.0	31.0	34.0	30.0	34.0
2	31.58778166081138	31.0	31.0	34.0	30.0	34.0
3	31.691225061242303	31.0	31.0	34.0	30.0	34.0
4	35.49364217281856	37.0	35.0	37.0	33.0	37.0
5	35.44956308187873	37.0	35.0	37.0	33.0	37.0
6	35.32786532532957	37.0	35.0	37.0	33.0	37.0
7	35.603119131266006	37.0	35.0	37.0	33.0	37.0
8	35.15845850737828	37.0	35.0	37.0	32.0	37.0
9	36.9786597079426	39.0	37.0	39.0	33.0	39.0
10	36.60160997335153	39.0	35.0	39.0	32.0	39.0
11	36.5419229315162	39.0	35.0	39.0	32.0	39.0
12	36.56803966226828	39.0	35.0	39.0	32.0	39.0
13	36.3214414148586	39.0	35.0	39.0	32.0	39.0
14	37.405566979255994	39.0	36.0	41.0	32.0	41.0
15	37.22306030177011	39.0	36.0	41.0	31.0	41.0
16	37.516642231217745	39.0	36.0	41.0	32.0	41.0
17	37.205497514960946	39.0	36.0	41.0	31.0	41.0
18	37.18819291402718	39.0	36.0	40.0	32.0	41.0
19	37.03332441977335	39.0	35.0	40.0	31.0	41.0
20	36.76401810989498	39.0	35.0	40.0	30.0	41.0
21	37.06582817729993	39.0	35.0	40.0	32.0	41.0
22	37.17688806105481	39.0	35.0	40.0	32.0	41.0
23	37.10416263251236	39.0	35.0	40.0	32.0	41.0
24	37.0265009359238	39.0	35.0	41.0	31.0	41.0
25	36.998549241271625	39.0	35.0	40.0	32.0	41.0
26	36.929659717163524	39.0	35.0	40.0	31.0	41.0
27	36.88427125499851	39.0	35.0	41.0	31.0	41.0
28	36.644597921603705	39.0	35.0	41.0	30.0	41.0
29	36.62264290127157	39.0	35.0	41.0	30.0	41.0
30	36.51957448508823	39.0	35.0	40.0	30.0	41.0
31	36.39390558388428	39.0	35.0	40.0	30.0	41.0
32	36.23106406390715	39.0	35.0	40.0	30.0	41.0
33	36.15785914731041	39.0	35.0	40.0	30.0	41.0
34	36.057553934722	39.0	35.0	41.0	30.0	41.0
35	36.02978973219363	39.0	35.0	41.0	29.0	41.0
36	35.778141492006995	39.0	35.0	40.0	28.0	41.0
37	35.79129052980356	39.0	35.0	40.0	28.0	41.0
38	35.46273056152354	38.0	35.0	40.0	26.0	41.0
39	35.6159147003046	38.0	35.0	40.0	27.0	41.0
40	35.497677863942194	38.0	35.0	40.0	26.0	41.0
41	35.422014034246516	38.0	35.0	40.0	26.0	41.0
42	35.557816731059454	39.0	35.0	40.0	26.0	41.0
43	35.50266638389166	39.0	35.0	40.0	26.0	41.0
44	35.548048698773925	39.0	35.0	40.0	26.0	41.0
45	35.408738977153625	38.0	35.0	40.0	26.0	41.0
46	35.27653551438925	38.0	35.0	40.0	25.0	41.0
47	34.971501197183315	38.0	34.0	40.0	25.0	41.0
48	35.00921477683827	38.0	34.0	40.0	25.0	41.0
49	35.01384060710564	38.0	34.0	40.0	25.0	41.0
50	34.83695254605083	38.0	34.0	40.0	24.0	41.0
51	33.43804000036884	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	5.0
13	7.0
14	9.0
15	19.0
16	26.0
17	72.0
18	169.0
19	299.0
20	783.0
21	1429.0
22	2141.0
23	2697.0
24	2983.0
25	3551.0
26	4243.0
27	4553.0
28	4814.0
29	5247.0
30	6365.0
31	8298.0
32	11329.0
33	15264.0
34	22791.0
35	28109.0
36	26883.0
37	36525.0
38	62101.0
39	74607.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.27495566272319	17.14845995198972	18.552191967345635	12.02439241794146
2	26.303915511745917	42.4488315552318	18.198723209373377	13.048529723648905
3	22.218124033723992	41.327259817978955	24.187713425972884	12.266902722324165
4	21.762610382145834	19.41188945956164	45.167159986107144	13.65834017218539
5	35.90566379895927	22.574359068932555	23.086735085923646	18.433242046184535
6	19.288021712202664	43.25504768754591	25.371372718973895	12.085557881277529
7	61.22755089181704	5.661647410303461	28.200044875164053	4.910756822715439
8	58.4821744168534	20.365025649537262	14.479924511367862	6.672875422241484
9	55.18415722290355	6.745720722797506	13.293806305267914	24.776315749031035
10	38.92059862239394	24.342010222931208	21.370106378727943	15.367284775946914
11	30.51480419367629	19.841584523600954	29.796186840511822	19.847424442210933
12	23.864981081737344	17.690035562030694	32.96295954780588	25.482023808426078
13	23.179559055408532	21.152799933609344	41.04048907781538	14.627151933166743
14	18.868162300559096	32.36790257786302	27.305615235425563	21.458319886152324
15	13.780671098857528	23.74019124196627	42.58837487359649	19.890762785579703
16	15.632540026494791	26.147467165826026	27.758055245630047	30.461937562049137
17	15.713376794622357	27.77434554491051	33.93699649912247	22.575281161344655
18	16.147682320722183	26.425016981868588	31.36528076177128	26.06201993563795
19	15.887959624646916	28.584250046873034	28.51878148561382	27.009008842866233
20	20.58448364361743	26.602673453266817	35.90043860862402	16.91240429449173
21	18.60813224034646	34.844028068493024	29.50972346448561	17.038116226674905
22	15.95588709900506	22.250704632284915	34.20563275518139	27.587775513528634
23	18.835889066135543	31.99476251509926	30.809566401411416	18.35978201735378
24	21.21888322314329	26.14039779066658	26.30053450623488	26.340184479955248
25	16.488241784925016	38.17647004582799	25.19463833998777	20.14064982925922
26	17.713087872333233	23.5779029774364	32.24003909671827	26.4689700535121
27	24.330023021573886	27.58132086664392	25.325575462506183	22.763080649276006
28	15.933756881114626	26.462822770764756	35.71448330551688	21.888937042603743
29	27.55335073014351	22.029095089243178	27.842887747543394	22.574666433069922
30	24.230129676929554	28.8636440477398	30.07496611310386	16.83126016222679
31	23.817339640445432	22.715131843846724	27.650477797551538	25.81705071815631
32	27.317602436782877	32.233277085696194	24.141916169505173	16.30720430801575
33	26.024214146741787	22.791358149913783	23.660891294525538	27.523536408818895
34	26.602058724992084	23.07751416180263	26.381986002637188	23.9384411105681
35	24.359529978761138	23.359367075768333	28.659246896390624	23.621856049079906
36	24.579910065253408	28.65955426052799	28.323297894248295	18.43723777997031
37	22.592186188899852	28.270738626758508	30.942347708754035	18.1947274755876
38	22.186772891712543	25.59021598477933	28.79018401890904	23.432827104599088
39	22.230111235081313	29.091400873528876	25.285003396373718	23.39348449501609
40	23.638453712497736	22.09425628636502	33.656065677568876	20.611224323568376
41	17.45275044798323	30.479457317879067	25.124866680805418	26.942925553332287
42	18.10897288126216	24.035875542113498	38.0021945799408	19.85295699668354
43	23.67779632208073	27.43101980347137	24.53165389568676	24.359529978761138
44	22.485838197370807	22.606939667493474	29.883785619661467	25.023436515474245
45	19.609524599888733	20.59186038291424	29.75930314402776	30.03931187316926
46	29.00165054541766	27.566260023912932	24.472025253037526	18.96006417763188
47	17.34732454886629	22.399776238907997	39.74587133122481	20.5070278810009
48	21.397461786953624	25.459893590535643	27.15869517776405	25.983949444746685
49	18.490104411597464	20.266361761442397	37.85035669608142	23.393177130878723
50	21.524710539823637	20.395147334999248	32.131232192090295	25.948909933086828
51	21.04891085517924	20.156632764402318	27.41472950419091	31.379726876227537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2738.0
1	1875.5
2	1013.0
3	2672.0
4	4331.0
5	2823.0
6	1315.0
7	1234.0
8	1153.0
9	1136.5
10	1120.0
11	1139.0
12	1158.0
13	1072.5
14	987.0
15	964.5
16	942.0
17	928.5
18	915.0
19	882.0
20	849.0
21	994.0
22	1139.0
23	1293.0
24	1447.0
25	1585.0
26	2198.5
27	2674.0
28	3019.0
29	3364.0
30	3843.0
31	4322.0
32	5216.5
33	6111.0
34	6818.5
35	7526.0
36	7903.0
37	8280.0
38	9180.0
39	10080.0
40	10968.5
41	11857.0
42	13364.0
43	14871.0
44	16875.0
45	18879.0
46	49201.0
47	79523.0
48	56205.0
49	32887.0
50	31019.5
51	29152.0
52	23740.5
53	18329.0
54	16198.5
55	14068.0
56	12813.5
57	11559.0
58	10064.0
59	8569.0
60	7936.5
61	7304.0
62	6386.5
63	5469.0
64	4594.5
65	3720.0
66	3126.0
67	2532.0
68	1969.0
69	1406.0
70	1154.0
71	902.0
72	697.0
73	492.0
74	396.0
75	220.5
76	141.0
77	127.5
78	114.0
79	81.0
80	48.0
81	31.0
82	14.0
83	14.0
84	14.0
85	8.5
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	325347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.13238868216656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6198999238731	23.85360505218722
2	8.911790545966934	5.5489065426219994
3	3.051699395962854	2.8502007520874546
4	1.637664679071214	2.039376532796024
5	1.104768141755032	1.7197035596396273
6	0.8508212282332195	1.5892858305836963
7	0.7588405459191839	1.653716317634045
8	0.6318672262811751	1.5737228867286426
9	0.5708800414503922	1.599557340719247
>10	5.739793901860201	32.00670973845739
>50	0.06798571421816602	1.4286764339153835
>100	0.03499264702405604	2.2914849469466345
>500	0.005998739489838178	1.2437888954086869
>1k	0.009997899149730297	7.210925260243506
>5k	0.0	0.0
>10k+	0.002999369744919089	13.390339910030457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	14753	4.534543118578011	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	14540	4.469074557318802	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	13727	4.219187513639284	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3084	0.9479109996403839	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	2866	0.8809056176943386	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	2786	0.8563164867049642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.8538575736060268	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2470	0.7591894192969353	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	2269	0.6974092276861321	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	2193	0.6740495532462264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	2128	0.6540708843173596	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	1409	0.433076069550357	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	1184	0.36391913864274145	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	825	0.2535754133279237	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	745	0.2289862823385493	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATG	707	0.21730644511859645	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	639	0.1964056837776282	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTAT	562	0.1727386452003553	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGT	518	0.1592146231561994	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	448	0.13769913354049676	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAGCTA	402	0.12356038322160648	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	402	0.12356038322160648	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	377	0.11587627978742697	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	359	0.11034372531481772	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	327	0.10050807291906794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21423280374492465	0.0
2	0.0	0.0	0.0	1.6526969666233284	0.0
3	0.0	0.0	0.0	2.378383694947241	0.0
4	0.0	0.0	0.0	3.926884219003095	0.0
5	0.0	0.0	0.0	9.486486735700652	0.0
6	0.0	0.0	0.0	10.726701029977225	0.0
7	0.0	0.0	0.0	12.385545279347896	0.0
8	0.0	0.0	0.0	14.005354283272936	0.0
9	0.0	0.0	0.0	14.447343912806941	0.0
10	0.0	0.0	0.0	19.815458571924744	0.0
11	0.0	0.0	0.0	21.04307093656926	0.0
12	0.0	0.0	0.0	26.655232720756608	0.0
13	0.0	0.0	0.0	27.27088308790307	0.0
14	0.0	0.0	0.0	27.602836356259623	0.0
15	0.0	0.0	0.0	28.547058986251603	0.0
16	0.0	0.0	0.0	29.19682677264582	0.0
17	0.0	0.0	0.0	29.902842196178234	0.0
18	0.0	0.0	0.0	30.63467620724949	0.0
19	0.0	0.0	0.0	31.853067647772992	0.0
20	0.0	0.0	0.0	32.399868448149206	0.0
21	0.0	0.0	0.0	32.89103633966196	0.0
22	0.0	0.0	0.0	33.491318499940064	0.0
23	3.0736413736718026E-4	0.0	0.0	34.01045652795323	0.0
24	3.0736413736718026E-4	0.0	0.0	34.436463222344145	0.0
25	3.0736413736718026E-4	0.0	0.0	34.82497149197626	0.0
26	3.0736413736718026E-4	0.0	0.0	35.2067177505863	0.0
27	3.0736413736718026E-4	0.0	0.0	35.69696354968695	0.0
28	3.0736413736718026E-4	0.0	0.0	36.067337335214404	0.0
29	3.0736413736718026E-4	0.0	0.0	36.46875489861594	0.0
30	3.0736413736718026E-4	0.0	0.0	36.90705615850154	0.0
31	3.0736413736718026E-4	0.0	0.0	37.3232272004967	0.0
32	3.0736413736718026E-4	0.0	0.0	37.71911220942563	0.0
33	6.147282747343605E-4	0.0	0.0	38.05997903776583	0.0
34	6.147282747343605E-4	0.0	0.0	38.447872579123214	0.0
35	6.147282747343605E-4	0.0	0.0	38.81640217982646	0.0
36	6.147282747343605E-4	0.0	0.0	39.17017830193608	0.0
37	6.147282747343605E-4	0.0	0.0	39.52118814680941	0.0
38	6.147282747343605E-4	0.0	0.0	39.895250301985264	0.0
39	6.147282747343605E-4	0.0	0.0	40.26408726682588	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGG	30	2.1606866E-6	45.000004	31
ACGGCTA	55	1.8189894E-12	45.000004	30
CGGCTAT	55	1.8189894E-12	45.000004	31
TTTTGCG	30	2.1606866E-6	45.000004	1
TGGTATG	30	2.1606866E-6	45.000004	1
ATTTACG	20	7.0258224E-4	45.0	1
GTGCACG	25	3.8846203E-5	45.0	1
CGGAATT	20	7.0258224E-4	45.0	13
TGCTTCG	20	7.0258224E-4	45.0	35
CGACGTT	25	3.8846203E-5	45.0	27
TTTACGG	20	7.0258224E-4	45.0	2
CACGGCC	20	7.0258224E-4	45.0	31
GCGAAGG	50	2.1827873E-11	45.0	2
TCGGGTG	20	7.0258224E-4	45.0	5
CCGCTTC	20	7.0258224E-4	45.0	20
GGATAGT	45	3.8380676E-10	45.0	8
TTCCCGT	20	7.0258224E-4	45.0	24
TCGGAAT	20	7.0258224E-4	45.0	12
GCTTCGC	20	7.0258224E-4	45.0	36
TTGCGAG	20	7.0258224E-4	45.0	1
>>END_MODULE