FastQCFastQC Report
Sat 14 Jan 2017
SRR2935425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249594
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185557.434072934445539No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC172706.919236840629182No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160416.426837183586144No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG73722.9535966409448946No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC72442.902313356891592No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT59282.375057092718575No Hit
CGTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT56322.256464498345313No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG52562.1058198514387367No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT29621.1867272450459547No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT29091.1654927602426342No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC28961.1602843017059705No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT22070.8842359992628027No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC21300.853385898699488No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT21160.847776789506158No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC19190.768848610142872No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC15440.6186046138929622No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT12050.48278404128304364No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC9980.3998493553530934No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC9770.3914356915630985No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC9480.3798168225197721No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA9150.36659535084978No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT8030.3217224773031403No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT7930.317715970736476No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT7220.28926977411315974No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG6980.27965415835316554No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.23838714071652364No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC5360.21474875197320448No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT5170.2071363894965424No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACTCACCATCG4220.16907457711323187No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT3700.14824074296657772No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG3680.14743944165324488No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT3440.13782382589325065No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT3410.13662187392325134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2810.1125828345232658No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC2540.10176526679327227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC253.8820028E-545.06
CTCGTAA253.8820028E-545.033
TGGACGG207.0226646E-445.02
CGGCCAC253.8820028E-545.031
ACCGAGG551.8189894E-1245.017
ACCTGGT351.2068267E-745.011
ACTAGGG207.0226646E-445.03
ACGGCCA207.0226646E-445.030
AACATGC253.8820028E-545.043
TACGAAT207.0226646E-445.012
TCGTAAC253.8820028E-545.034
ACATGCA253.8820028E-545.044
CGTAACC253.8820028E-545.035
AGTAGGG406.7775545E-945.03
AGCTACG207.0226646E-445.09
GCTCGTA253.8820028E-545.032
AAGCTAC207.0226646E-445.08
TGATCCG302.1586511E-644.9999964
GTTGATC302.1586511E-644.99999616
TGCTTGT302.1586511E-644.99999637
Produced by FastQC (version 0.11.5)