##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	249594
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433932706715705	31.0	31.0	34.0	30.0	34.0
2	31.517015633388624	31.0	31.0	34.0	30.0	34.0
3	31.546775964165807	31.0	31.0	34.0	30.0	34.0
4	35.42764249140604	37.0	35.0	37.0	33.0	37.0
5	35.37623500564917	37.0	35.0	37.0	33.0	37.0
6	35.195192993421315	37.0	35.0	37.0	32.0	37.0
7	35.36284526070338	37.0	35.0	37.0	33.0	37.0
8	34.77631673838314	36.0	35.0	37.0	32.0	37.0
9	36.68622242521855	39.0	35.0	39.0	32.0	39.0
10	36.67087750506823	39.0	35.0	39.0	32.0	39.0
11	36.77125251408287	39.0	35.0	39.0	32.0	39.0
12	36.822631954293776	39.0	37.0	39.0	32.0	39.0
13	36.65072477703791	39.0	35.0	39.0	32.0	39.0
14	37.79621304999319	40.0	37.0	41.0	32.0	41.0
15	37.59569540934478	40.0	36.0	41.0	32.0	41.0
16	37.64563250719168	39.0	36.0	41.0	32.0	41.0
17	37.27100411067574	39.0	36.0	41.0	32.0	41.0
18	36.79279149338526	38.0	36.0	40.0	31.0	41.0
19	36.254513329647345	37.0	35.0	40.0	31.0	41.0
20	35.87460435747654	36.0	35.0	40.0	30.0	41.0
21	36.1210646089249	37.0	35.0	40.0	31.0	41.0
22	36.270779746308	37.0	35.0	40.0	31.0	41.0
23	36.19228026314735	37.0	35.0	40.0	31.0	41.0
24	36.02306545830429	37.0	35.0	40.0	31.0	41.0
25	35.85481221503722	37.0	35.0	40.0	31.0	41.0
26	35.78684984414689	37.0	35.0	40.0	31.0	41.0
27	35.7870782150212	37.0	35.0	40.0	31.0	41.0
28	35.5559107991378	36.0	35.0	40.0	30.0	41.0
29	35.51163890157616	36.0	35.0	40.0	30.0	41.0
30	35.37684399464731	36.0	35.0	40.0	30.0	41.0
31	35.32608556295424	37.0	35.0	40.0	29.0	41.0
32	35.02944782326498	37.0	35.0	40.0	26.0	41.0
33	34.586208001794915	36.0	34.0	40.0	24.0	41.0
34	34.40867568931945	37.0	34.0	40.0	23.0	41.0
35	34.18492431709095	37.0	34.0	40.0	21.0	41.0
36	34.04373101917514	37.0	34.0	40.0	21.0	41.0
37	33.97893378847248	37.0	34.0	40.0	20.0	41.0
38	33.73477727829996	36.0	33.0	40.0	18.0	41.0
39	33.48125355577458	36.0	33.0	40.0	17.0	41.0
40	33.189888378727055	35.0	33.0	40.0	15.0	41.0
41	33.00750819330593	35.0	33.0	40.0	15.0	41.0
42	33.17101773279806	36.0	33.0	40.0	15.0	41.0
43	33.09243411299951	36.0	33.0	40.0	15.0	41.0
44	32.95447807238956	36.0	33.0	40.0	13.0	41.0
45	32.94817183105363	36.0	33.0	40.0	13.0	41.0
46	32.62524740178049	35.0	32.0	40.0	12.0	41.0
47	32.33025232978357	35.0	31.0	39.0	10.0	41.0
48	32.29584445138906	35.0	31.0	40.0	10.0	41.0
49	32.225277851230395	35.0	31.0	40.0	10.0	41.0
50	32.00865004767743	35.0	31.0	40.0	10.0	41.0
51	30.607983364984737	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	5.0
12	7.0
13	11.0
14	33.0
15	33.0
16	44.0
17	84.0
18	189.0
19	375.0
20	694.0
21	1114.0
22	1692.0
23	2259.0
24	3254.0
25	4358.0
26	5883.0
27	6717.0
28	6389.0
29	6708.0
30	7103.0
31	8672.0
32	10933.0
33	13966.0
34	18971.0
35	30806.0
36	24702.0
37	23410.0
38	36131.0
39	35035.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.978124474146014	21.857496574436887	8.97096885341795	29.193410097999152
2	35.248844122855516	47.59208955343478	9.229388526967796	7.929677796741909
3	11.028710626056716	47.55643164499146	32.40302250855389	9.011835220397927
4	10.993052717613404	23.564669022492527	53.21081436252474	12.231463897369329
5	25.23898811670152	24.328309174098735	33.93190541439298	16.500797294806766
6	13.953059769064962	38.64836494467014	37.424777839210876	9.973797447054016
7	35.74805484106188	15.714320055770573	41.65604942426501	6.8815756789025375
8	29.960255454858693	26.676522672820663	32.763207448897006	10.600014423423639
9	26.471389536607447	16.583731980736715	31.29882929878122	25.646049183874613
10	21.651562136910343	24.30787599060875	37.037749304871106	17.002812567609798
11	17.69193169707605	24.936897521575037	39.2753832223531	18.09578755899581
12	12.226255438832665	23.053839435242836	44.731844515493165	19.98806061043134
13	14.377348814474708	27.718615030810035	47.71348670240471	10.190549452310552
14	10.077565967130619	39.42562721860301	36.39630760354816	14.100499210718207
15	6.884380233499203	31.77079577233427	46.45744689375546	14.887377100411067
16	7.413639750955552	36.08700529660168	31.27438960872457	25.224965343718196
17	7.550261624878803	36.51289694463809	34.23680056411612	21.70004086636698
18	8.341146021138329	36.109441733375	32.081300031250755	23.46811221423592
19	8.609181310448168	36.02209989022172	31.514780002724425	23.853938796605686
20	11.21341057877994	34.145852865052845	39.03459217769658	15.606144378470635
21	10.412109265447086	37.37429585647091	36.88430010336787	15.329294774714135
22	9.839178826414097	28.904941625199328	37.99690697693053	23.258972571456045
23	13.901776485011657	33.91547873746965	37.36427959005425	14.818465187464442
24	18.460379656562257	31.067253219228025	29.57202496854892	20.90034215566079
25	12.868498441468946	42.043077958604776	26.84679920190389	18.241624398022388
26	14.265166630608109	29.437406347909008	31.57447695056772	24.722950070915168
27	19.231231519988462	31.96390938884749	25.962162551984424	22.842696539179627
28	10.683349760010257	32.683477968220394	33.189099096933425	23.444073174835932
29	13.740714921031755	33.53205605903988	28.99188281769594	23.735346202232424
30	9.838377525100764	33.67548899412646	32.598540029007104	23.887593451765667
31	11.645712637323012	28.384897072846304	32.626184924317094	27.343205365513594
32	13.306008958548682	34.70355857913251	26.318741636417542	25.671690825901262
33	9.546703847047604	36.40752582193482	24.424465331698677	29.62130499931889
34	11.119257674463329	33.54647948267987	25.650456341097943	29.68380650175886
35	9.170092229781163	32.316481966713944	24.470139506558652	34.04328629694624
36	9.704960856430844	40.46972282987572	29.388126317139037	20.437189996554405
37	10.694167327740251	29.138120307379182	32.574500989607124	27.593211375273448
38	13.609301505645169	32.23514988341066	29.804001698758785	24.35154691218539
39	17.969983252802553	29.73388783384216	29.374103544155712	22.92202536919958
40	13.29639334278869	33.36979254308998	34.08575526655288	19.24805884756845
41	12.775547489122335	37.18358614389769	29.6128913355289	20.427975031451076
42	15.501975207737365	28.011490660833193	35.81416219941185	20.672371932017597
43	19.947594894108033	30.0568122631153	26.67812527544733	23.31746756732934
44	17.643452967619414	26.04309398463104	29.068407093119227	27.24504595463032
45	16.4431036002468	22.943660504659565	30.517961168938356	30.095274726155274
46	23.52420330616922	26.939349503593835	29.244292731395788	20.292154458841157
47	12.522336274109152	24.107550662275536	41.89884372220486	21.47126934141045
48	14.660208178081206	25.73699688293789	33.96956657611962	25.63322836286129
49	11.984262442206143	22.018958789073455	43.66891832335713	22.32786044536327
50	14.208674888018141	21.867913491510212	39.78901736419946	24.134394256272184
51	15.145796773960912	21.495709031467104	34.086957218522876	29.271536976049106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19902.0
1	12364.5
2	4827.0
3	12696.0
4	20565.0
5	12323.5
6	4082.0
7	3779.5
8	3477.0
9	3493.0
10	3509.0
11	3343.5
12	3178.0
13	2962.0
14	2746.0
15	2471.0
16	2196.0
17	2003.5
18	1811.0
19	1613.0
20	1415.0
21	1288.0
22	1161.0
23	1106.0
24	1051.0
25	1019.5
26	1001.5
27	1015.0
28	1087.0
29	1159.0
30	1286.5
31	1414.0
32	1550.5
33	1687.0
34	1947.0
35	2207.0
36	2734.0
37	3261.0
38	3287.0
39	3313.0
40	3908.5
41	4504.0
42	5941.5
43	7379.0
44	9806.0
45	12233.0
46	37972.5
47	63712.0
48	44143.5
49	24575.0
50	22600.5
51	20626.0
52	14290.5
53	7955.0
54	6832.5
55	5710.0
56	5109.5
57	4509.0
58	3966.5
59	3424.0
60	3190.0
61	2956.0
62	2685.0
63	2414.0
64	1974.0
65	1534.0
66	1317.0
67	1100.0
68	910.0
69	720.0
70	617.5
71	515.0
72	428.0
73	341.0
74	282.5
75	155.0
76	86.0
77	70.5
78	55.0
79	43.0
80	31.0
81	23.0
82	15.0
83	9.5
84	4.0
85	4.0
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	249594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.79328028718639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70867616721598	27.612041956136768
2	8.925141678324994	6.0321962867697145
3	3.592345813672255	3.641914469097815
4	1.8518957626917698	2.5032653028518315
5	1.1298698219239798	1.909100379015521
6	0.699499679889977	1.418303324599149
7	0.47068029307850995	1.1134081748759987
8	0.36279135940056434	0.9807928075194116
9	0.22881938681146705	0.6959301906295824
>10	0.8844521376235981	4.909573146790388
>50	0.06876437531121807	1.6631008758223356
>100	0.04386692907784601	2.7725025441316697
>500	0.013041519455575842	3.406331882977956
>1k	0.010670334100016598	7.968140259781887
>5k	0.0059279633888981095	12.59325144033911
>10k+	0.003556778033338866	20.780146958660865
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18555	7.434072934445539	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	17270	6.919236840629182	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16041	6.426837183586144	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	7372	2.9535966409448946	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	7244	2.902313356891592	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	5928	2.375057092718575	No Hit
CGTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	5632	2.256464498345313	No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	5256	2.1058198514387367	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	2962	1.1867272450459547	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	2909	1.1654927602426342	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	2896	1.1602843017059705	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	2207	0.8842359992628027	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	2130	0.853385898699488	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	2116	0.847776789506158	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	1919	0.768848610142872	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	1544	0.6186046138929622	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT	1205	0.48278404128304364	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	998	0.3998493553530934	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	977	0.3914356915630985	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	948	0.3798168225197721	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA	915	0.36659535084978	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	803	0.3217224773031403	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	793	0.317715970736476	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	722	0.28926977411315974	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	698	0.27965415835316554	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	595	0.23838714071652364	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	536	0.21474875197320448	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT	517	0.2071363894965424	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACTCACCATCG	422	0.16907457711323187	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT	370	0.14824074296657772	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG	368	0.14743944165324488	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	344	0.13782382589325065	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	341	0.13662187392325134	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	281	0.1125828345232658	No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	254	0.10176526679327227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0065065666642627E-4	0.0	0.0	0.34415891407646015	0.0
2	4.0065065666642627E-4	0.0	0.0	2.157904436805372	0.0
3	4.0065065666642627E-4	0.0	0.0	4.713254325023839	0.0
4	4.0065065666642627E-4	0.0	0.0	10.011057958123994	0.0
5	4.0065065666642627E-4	0.0	0.0	19.392293083968365	0.0
6	4.0065065666642627E-4	0.0	0.0	22.972106701282883	0.0
7	4.0065065666642627E-4	0.0	0.0	25.501414296818034	0.0
8	4.0065065666642627E-4	0.0	0.0	27.60603219628677	0.0
9	4.0065065666642627E-4	0.0	0.0	28.470235662716252	0.0
10	4.0065065666642627E-4	0.0	0.0	32.55166390217713	0.0
11	4.0065065666642627E-4	0.0	0.0	33.6021699239565	0.0
12	4.0065065666642627E-4	0.0	0.0	37.599862176174106	0.0
13	4.0065065666642627E-4	0.0	0.0	38.445635712396935	0.0
14	4.0065065666642627E-4	0.0	0.0	38.914797631353316	0.0
15	4.0065065666642627E-4	0.0	0.0	39.545021114289604	0.0
16	4.0065065666642627E-4	0.0	0.0	40.082293644879286	0.0
17	4.0065065666642627E-4	0.0	0.0	40.595527136068974	0.0
18	4.0065065666642627E-4	0.0	0.0	41.16004391131197	0.0
19	4.0065065666642627E-4	0.0	0.0	41.9425146437815	0.0
20	4.0065065666642627E-4	0.0	0.0	42.3375561912546	0.0
21	4.0065065666642627E-4	0.0	0.0	42.5535068951978	0.0
22	4.0065065666642627E-4	0.0	0.0	42.82434673910431	0.0
23	4.0065065666642627E-4	0.0	0.0	43.055522168000834	0.0
24	4.0065065666642627E-4	0.0	0.0	43.42532272410394	0.0
25	4.0065065666642627E-4	0.0	0.0	43.61362853273717	0.0
26	4.0065065666642627E-4	0.0	0.0	43.81515581304038	0.0
27	4.0065065666642627E-4	0.0	0.0	44.11604445619686	0.0
28	4.0065065666642627E-4	0.0	0.0	44.261881295223446	0.0
29	4.0065065666642627E-4	0.0	0.0	44.47582874588331	0.0
30	4.0065065666642627E-4	0.0	0.0	44.68857424457319	0.0
31	4.0065065666642627E-4	0.0	0.0	44.884893066339735	0.0
32	4.0065065666642627E-4	0.0	0.0	45.06758976577962	0.0
33	4.0065065666642627E-4	0.0	0.0	45.23065458304286	0.0
34	4.0065065666642627E-4	0.0	0.0	45.39692460555943	0.0
35	4.0065065666642627E-4	0.0	0.0	45.56279397741933	0.0
36	4.0065065666642627E-4	0.0	0.0	45.70742886447591	0.0
37	4.0065065666642627E-4	0.0	0.0	45.85126245021915	0.0
38	4.0065065666642627E-4	0.0	0.0	46.01112206222906	0.0
39	4.0065065666642627E-4	0.0	0.0	46.15535629862897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	25	3.8820028E-5	45.0	6
CTCGTAA	25	3.8820028E-5	45.0	33
TGGACGG	20	7.0226646E-4	45.0	2
CGGCCAC	25	3.8820028E-5	45.0	31
ACCGAGG	55	1.8189894E-12	45.0	17
ACCTGGT	35	1.2068267E-7	45.0	11
ACTAGGG	20	7.0226646E-4	45.0	3
ACGGCCA	20	7.0226646E-4	45.0	30
AACATGC	25	3.8820028E-5	45.0	43
TACGAAT	20	7.0226646E-4	45.0	12
TCGTAAC	25	3.8820028E-5	45.0	34
ACATGCA	25	3.8820028E-5	45.0	44
CGTAACC	25	3.8820028E-5	45.0	35
AGTAGGG	40	6.7775545E-9	45.0	3
AGCTACG	20	7.0226646E-4	45.0	9
GCTCGTA	25	3.8820028E-5	45.0	32
AAGCTAC	20	7.0226646E-4	45.0	8
TGATCCG	30	2.1586511E-6	44.999996	4
GTTGATC	30	2.1586511E-6	44.999996	16
TGCTTGT	30	2.1586511E-6	44.999996	37
>>END_MODULE