FastQCFastQC Report
Sat 14 Jan 2017
SRR2935422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337190
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC58201.7260298348112342No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC56321.6702749191850292No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG54851.626679320264539No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC19340.573563866069575No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT19310.5726741599691568No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT10910.32355645185207155No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT9900.2936030131379934No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTGACTT8960.265725555324891No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTC7970.23636525401109168No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTA7860.2331029983095584No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7780.2307304487084433No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.2259853495062131No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC7550.22390936860523739No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5970.1770515139832142No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT5830.1728995521812628No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5040.1494706248702512No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4120.12218630445742756No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3680.10913728165129453No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC3580.10617159464990066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG207.026186E-445.0000041
CGAGTAA207.026186E-445.00000439
ACGTTTG207.026186E-445.0000041
CGTTGAT302.1609212E-645.00000425
CGTTCTA207.026186E-445.00000420
ACGGGCC207.026186E-445.0000045
CCCACCG207.026186E-445.00000418
CGAATGC207.026186E-445.00000445
CGTACCA207.026186E-445.00000439
TCGTACC207.026186E-445.00000438
TACTAGT207.026186E-445.00000443
AGTACGA207.026186E-445.00000411
GTACGAA207.026186E-445.00000412
CGACGGT253.884923E-545.028
AATGCGG253.884923E-545.02
ATTGCGG253.884923E-545.02
TACCGGG253.884923E-545.03
CCGATGA3250.043.61538718
ATGGGAA1850.042.5675665
CGATGAA3700.040.74324419
Produced by FastQC (version 0.11.5)