##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935422.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 337190 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74870547762389 31.0 31.0 34.0 30.0 34.0 2 31.809543580770484 33.0 31.0 34.0 30.0 34.0 3 31.90061389720929 33.0 31.0 34.0 30.0 34.0 4 35.648032266674576 37.0 35.0 37.0 33.0 37.0 5 35.65086449776091 37.0 35.0 37.0 33.0 37.0 6 35.584231442213586 37.0 35.0 37.0 33.0 37.0 7 35.837198612058486 37.0 35.0 37.0 35.0 37.0 8 35.538479788843084 37.0 35.0 37.0 33.0 37.0 9 37.32927726207776 39.0 37.0 39.0 34.0 39.0 10 36.86147869153889 39.0 37.0 39.0 32.0 39.0 11 36.87035202704706 39.0 37.0 39.0 33.0 39.0 12 36.8550965331119 39.0 35.0 39.0 33.0 39.0 13 36.66558320234883 39.0 35.0 39.0 32.0 39.0 14 37.706512648655064 40.0 36.0 41.0 33.0 41.0 15 37.78225926035766 40.0 36.0 41.0 33.0 41.0 16 37.824671550164595 40.0 36.0 41.0 33.0 41.0 17 37.745968148521605 39.0 36.0 41.0 33.0 41.0 18 37.71202289510365 39.0 36.0 41.0 33.0 41.0 19 37.64377947151458 39.0 36.0 41.0 33.0 41.0 20 37.61654260209377 39.0 36.0 41.0 32.0 41.0 21 37.62696699190367 39.0 36.0 41.0 33.0 41.0 22 37.727652065601 39.0 36.0 41.0 33.0 41.0 23 37.71688958747294 39.0 36.0 41.0 33.0 41.0 24 37.69718852872268 39.0 36.0 41.0 33.0 41.0 25 37.49529938610279 39.0 35.0 41.0 33.0 41.0 26 37.49414869954625 39.0 36.0 41.0 33.0 41.0 27 37.5108514487381 39.0 36.0 41.0 33.0 41.0 28 37.405614045493635 39.0 36.0 41.0 33.0 41.0 29 37.40094308846644 39.0 36.0 41.0 33.0 41.0 30 37.292292179483375 39.0 36.0 41.0 32.0 41.0 31 37.35174827248732 39.0 36.0 41.0 33.0 41.0 32 37.270369821169076 39.0 35.0 41.0 32.0 41.0 33 37.171582787152644 40.0 35.0 41.0 32.0 41.0 34 37.06342418221181 39.0 35.0 41.0 32.0 41.0 35 37.08686200658383 40.0 35.0 41.0 32.0 41.0 36 37.05804146030428 40.0 35.0 41.0 32.0 41.0 37 37.00408671668792 39.0 35.0 41.0 32.0 41.0 38 36.952795159998814 39.0 35.0 41.0 31.0 41.0 39 36.78938877190901 39.0 35.0 41.0 31.0 41.0 40 36.68179957887244 39.0 35.0 41.0 31.0 41.0 41 36.44848305109879 39.0 35.0 41.0 30.0 41.0 42 36.63332839052166 39.0 35.0 41.0 31.0 41.0 43 36.600652451140306 39.0 35.0 41.0 31.0 41.0 44 36.525561256265014 39.0 35.0 41.0 31.0 41.0 45 36.511720395029506 39.0 35.0 41.0 31.0 41.0 46 36.38585070731635 39.0 35.0 40.0 30.0 41.0 47 36.28281384382692 39.0 35.0 40.0 30.0 41.0 48 36.19442747412438 38.0 35.0 40.0 30.0 41.0 49 36.174714552626114 39.0 35.0 40.0 30.0 41.0 50 36.04209792698479 38.0 35.0 40.0 30.0 41.0 51 34.79631365105727 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 5.0 10 9.0 11 2.0 12 7.0 13 9.0 14 11.0 15 22.0 16 30.0 17 52.0 18 94.0 19 205.0 20 406.0 21 637.0 22 1047.0 23 1493.0 24 1855.0 25 2174.0 26 2671.0 27 3164.0 28 3547.0 29 4064.0 30 5298.0 31 6916.0 32 9163.0 33 12864.0 34 22481.0 35 28458.0 36 26020.0 37 38375.0 38 68131.0 39 97937.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.56635724665619 19.13283312079243 22.61336338562828 10.687446246923098 2 30.0587206026276 32.046620599661914 22.007473531243512 15.887185266466977 3 29.302470417272158 31.06379192739998 24.11844953883567 15.515288116492185 4 27.600759215872355 23.370206708383996 31.842581333966013 17.186452741777632 5 29.77312494439337 27.380408671668793 22.30997360538569 20.53649277855215 6 23.634152851508052 39.15626204810344 23.375841513686645 13.83374358670186 7 73.27678756784009 6.593611910198997 15.326966991903673 4.802633530057237 8 72.43898098994632 12.540110916693854 9.539428808683532 5.481479284676295 9 65.799994068626 8.91099973308817 11.255968445090305 14.033037753195526 10 39.74317150567929 27.87686467570213 18.023369613570985 14.356594205047598 11 32.381743230819424 23.21302529731012 24.74331979002936 19.661911681841097 12 28.15119072333106 20.491710904831102 29.474183694652865 21.88291467718497 13 24.951214448827074 24.896942376701563 32.122245618197454 18.02959755627391 14 18.614431032948783 32.508971203179215 27.290548355526557 21.586049408345442 15 17.51683027373291 26.527477090067915 35.89756517097186 20.058127465227322 16 17.973842640647707 26.01708235712803 30.910762478128056 25.098312524096205 17 19.091016934072776 26.530442777069307 29.52163468667517 24.856905602182746 18 19.17731842581334 27.35104837035499 28.675227616477354 24.79640558735431 19 22.219816720543314 27.41451407218482 26.373557934695572 23.99211127257629 20 24.482932471306977 26.116729440374865 29.827990153919153 19.572347934399005 21 23.127020374269698 29.950176458376582 27.50734007532845 19.415463092025266 22 20.230137311308162 25.06480026098046 28.37865891633797 26.326403511373407 23 20.753581067054185 29.470328301551053 27.968207835345062 21.807882796049704 24 22.291882914677185 26.224383878525458 27.717903852427416 23.76582935436994 25 20.984608084462767 30.98727720276402 25.886888697766835 22.141226015006378 26 18.80156588273674 26.52925650226875 28.453394228773092 26.21578338622142 27 21.87223820397995 28.19360004745099 25.867018594857498 24.067143153711555 28 17.991933331356208 27.524541059936535 31.348201310833655 23.135324297873602 29 20.588985438476822 24.820427652065604 30.845813932797533 23.744772976660045 30 20.418161867196535 27.415403778285242 29.107921349980725 23.058513004537502 31 22.199056911533557 26.431685399922895 24.648714374684896 26.72054331385866 32 23.154601263382663 28.709333016993387 25.875915655861682 22.26015006376227 33 23.453245944423028 25.267949820575936 25.25875619087162 26.020048044129425 34 21.100863014917408 27.746671016340933 27.809543580770484 23.342922387971175 35 20.091046590942792 24.79343990035292 26.21904564192295 28.89646786678134 36 21.524956256116727 25.64251608885198 28.764198226519174 24.068329428512115 37 19.232183635339126 26.652925650226877 30.58542661407515 23.529464100358847 38 20.926480619235445 26.54497464337614 25.640143539250865 26.888401198137547 39 22.967762982294847 25.360182686319288 29.25412971914944 22.417924612236426 40 23.211245885109285 24.06921913461253 29.238115009341914 23.48141997093627 41 17.905928408315784 27.891396542008955 29.12838459029034 25.07429045938492 42 19.401820931818854 25.292268453987365 29.892048993149263 25.413861621044514 43 22.21269907173997 23.342625819271035 29.07529879296539 25.369376316023605 44 21.709421987603427 24.595925146060086 28.427889320561107 25.26676354577538 45 20.70761291853258 24.124084344138318 26.857261484622914 28.311041252706186 46 23.87555977342151 26.62682760461461 26.50019276965509 22.997419852308788 47 19.920519588362644 24.211868679379577 33.20620421720692 22.66140751505086 48 20.529968267149084 25.777751416115542 27.610249414276815 26.082030902458552 49 18.648239864764673 23.557044989471812 32.64865506094487 25.14606008481865 50 20.15451229277262 23.33135620866574 31.12814733533023 25.385984163231413 51 20.133455915062722 22.91378747886948 27.53076900263946 29.421987603428335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 321.0 1 462.5 2 604.0 3 1015.5 4 1427.0 5 1022.5 6 618.0 7 601.5 8 585.0 9 611.5 10 638.0 11 657.0 12 676.0 13 719.0 14 762.0 15 761.0 16 760.0 17 659.0 18 558.0 19 624.5 20 691.0 21 832.0 22 973.0 23 1202.5 24 1432.0 25 1599.0 26 2603.0 27 3440.0 28 4037.0 29 4634.0 30 5018.5 31 5403.0 32 6311.0 33 7219.0 34 8188.0 35 9157.0 36 9966.5 37 10776.0 38 11300.0 39 11824.0 40 12975.0 41 14126.0 42 16549.5 43 18973.0 44 20232.5 45 21492.0 46 35875.0 47 50258.0 48 41977.5 49 33697.0 50 32239.0 51 30781.0 52 26047.0 53 21313.0 54 19027.5 55 16742.0 56 15050.5 57 13359.0 58 13135.5 59 12912.0 60 12353.0 61 11794.0 62 10594.0 63 9394.0 64 8300.5 65 7207.0 66 5828.0 67 4449.0 68 3468.0 69 2487.0 70 2015.5 71 1544.0 72 1251.5 73 959.0 74 907.5 75 560.5 76 265.0 77 209.5 78 154.0 79 140.0 80 126.0 81 70.5 82 15.0 83 14.5 84 14.0 85 7.5 86 1.0 87 3.5 88 6.0 89 3.0 90 0.0 91 0.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 337190.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.526083217177256 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69641117327413 22.054924523265814 2 9.382376279392119 5.540496456004034 3 3.2312498116694623 2.8621845250452265 4 1.648268865697727 1.9466769477149382 5 0.9853453730953504 1.4546694741836947 6 0.7523177211502726 1.3327797384264066 7 0.5966311433421388 1.2331326551795723 8 0.5333520826846393 1.2598238381921172 9 0.4931749013147983 1.3105370859159524 >10 7.488022177804115 47.05774192591714 >50 0.12555369178075312 2.4979981612740594 >100 0.05122590624654727 2.747412438091284 >500 0.01004429534246025 2.2088436786381567 >1k 0.003013288602738075 1.4697944778908034 >5k 0.003013288602738075 5.022984074260803 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC 5820 1.7260298348112342 No Hit GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC 5632 1.6702749191850292 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG 5485 1.626679320264539 No Hit GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC 1934 0.573563866069575 No Hit GAATGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT 1931 0.5726741599691568 No Hit GAATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT 1091 0.32355645185207155 No Hit GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT 990 0.2936030131379934 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTGACTT 896 0.265725555324891 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTC 797 0.23636525401109168 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTA 786 0.2331029983095584 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 778 0.2307304487084433 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 762 0.2259853495062131 No Hit CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC 755 0.22390936860523739 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 597 0.1770515139832142 No Hit CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT 583 0.1728995521812628 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 504 0.1494706248702512 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 412 0.12218630445742756 No Hit GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 368 0.10913728165129453 No Hit AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC 358 0.10617159464990066 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.965687001393873E-4 0.0 0.0 0.18802455588837155 0.0 2 2.965687001393873E-4 0.0 0.0 1.2393605978824995 0.0 3 2.965687001393873E-4 0.0 0.0 1.58278715264391 0.0 4 2.965687001393873E-4 0.0 0.0 2.3019662504819243 0.0 5 2.965687001393873E-4 0.0 0.0 5.059165455677808 0.0 6 5.931374002787746E-4 0.0 0.0 6.302974584062398 0.0 7 5.931374002787746E-4 0.0 0.0 7.229751771997983 0.0 8 5.931374002787746E-4 0.0 0.0 8.294433405498383 0.0 9 5.931374002787746E-4 0.0 0.0 8.60612710934488 0.0 10 5.931374002787746E-4 0.0 0.0 10.986684065363741 0.0 11 5.931374002787746E-4 0.0 0.0 12.085471099380172 0.0 12 5.931374002787746E-4 0.0 0.0 14.792550194252499 0.0 13 5.931374002787746E-4 0.0 0.0 15.313324831697262 0.0 14 5.931374002787746E-4 0.0 0.0 15.575491562620481 0.0 15 5.931374002787746E-4 0.0 0.0 16.110798066372077 0.0 16 5.931374002787746E-4 0.0 0.0 16.7178741955574 0.0 17 5.931374002787746E-4 0.0 0.0 17.408582698182034 0.0 18 5.931374002787746E-4 0.0 0.0 18.132506895222278 0.0 19 5.931374002787746E-4 0.0 0.0 18.846051187757645 0.0 20 5.931374002787746E-4 0.0 0.0 19.33272042468638 0.0 21 8.897061004181618E-4 0.0 0.0 19.833624959221805 0.0 22 8.897061004181618E-4 0.0 0.0 20.423500103799046 0.0 23 8.897061004181618E-4 0.0 0.0 20.973635042557607 0.0 24 8.897061004181618E-4 0.0 0.0 21.37934102434829 0.0 25 0.0011862748005575493 0.0 0.0 21.780301906936742 0.0 26 0.0011862748005575493 0.0 0.0 22.19401524363119 0.0 27 0.0011862748005575493 0.0 0.0 22.626115839734275 0.0 28 0.0011862748005575493 0.0 0.0 23.014917405617012 0.0 29 0.0011862748005575493 0.0 0.0 23.41231946380379 0.0 30 0.0011862748005575493 0.0 0.0 23.826625937898513 0.0 31 0.0014828435006969364 0.0 0.0 24.22580740828613 0.0 32 0.0014828435006969364 0.0 0.0 24.600077107862035 0.0 33 0.0014828435006969364 0.0 0.0 25.028025742163173 0.0 34 0.0017794122008363237 0.0 0.0 25.4114890714434 0.0 35 0.0017794122008363237 0.0 0.0 25.859011239953734 0.0 36 0.0017794122008363237 0.0 0.0 26.295560366558913 0.0 37 0.0017794122008363237 0.0 0.0 26.690886443844718 0.0 38 0.0017794122008363237 0.0 0.0 27.073756635724667 0.0 39 0.002075980900975711 0.0 0.0 27.48895281591981 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCACG 20 7.026186E-4 45.000004 1 CGAGTAA 20 7.026186E-4 45.000004 39 ACGTTTG 20 7.026186E-4 45.000004 1 CGTTGAT 30 2.1609212E-6 45.000004 25 CGTTCTA 20 7.026186E-4 45.000004 20 ACGGGCC 20 7.026186E-4 45.000004 5 CCCACCG 20 7.026186E-4 45.000004 18 CGAATGC 20 7.026186E-4 45.000004 45 CGTACCA 20 7.026186E-4 45.000004 39 TCGTACC 20 7.026186E-4 45.000004 38 TACTAGT 20 7.026186E-4 45.000004 43 AGTACGA 20 7.026186E-4 45.000004 11 GTACGAA 20 7.026186E-4 45.000004 12 CGACGGT 25 3.884923E-5 45.0 28 AATGCGG 25 3.884923E-5 45.0 2 ATTGCGG 25 3.884923E-5 45.0 2 TACCGGG 25 3.884923E-5 45.0 3 CCGATGA 325 0.0 43.615387 18 ATGGGAA 185 0.0 42.567566 5 CGATGAA 370 0.0 40.743244 19 >>END_MODULE