SRR2935419.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935419.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458561
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	13428	2.928290892596623	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	12185	2.6572255381508674	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	11878	2.590276975146164	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3945	0.8602999382852009	Illumina PCR Primer Index 5 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	2739	0.5973033031592306	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2563	0.5589223680164689	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2096	0.4570820457910725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	1988	0.43353010831710503	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	1897	0.41368542026033617	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1894	0.4130311997749481	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1637	0.35698631152671073	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1603	0.3495718126923136	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1259	0.27455453036782457	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1007	0.21960000959523376	Illumina PCR Primer Index 5 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	780	0.17009732620087623	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATG	618	0.134769419989925	No Hit
ACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	592	0.12909950911656246	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	487	0.10620179212798297	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGA	25	3.887125E-5	45.000004	41
TTGGACG	25	3.887125E-5	45.000004	1
GGCGTAA	25	3.887125E-5	45.000004	29
TACGCGG	50	2.1827873E-11	45.000004	2
CCGGTGA	25	3.887125E-5	45.000004	18
AGCTAGC	25	3.887125E-5	45.000004	45
GTAACGA	25	3.887125E-5	45.000004	10
GACGTAA	20	7.0288463E-4	45.0	16
ATTGTCG	20	7.0288463E-4	45.0	1
GCTTGCG	20	7.0288463E-4	45.0	1
AGACGTA	20	7.0288463E-4	45.0	15
TCGGATT	20	7.0288463E-4	45.0	42
ACGATGA	40	6.7993824E-9	45.0	18
ATAAGCC	20	7.0288463E-4	45.0	12
CGAATAT	20	7.0288463E-4	45.0	14
ACGTAAT	20	7.0288463E-4	45.0	17
ACTCATT	20	7.0288463E-4	45.0	43
TAGTGCG	20	7.0288463E-4	45.0	1
ACGAGAC	20	7.0288463E-4	45.0	12
TCGAAGG	20	7.0288463E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content