##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935419.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458561
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.457492023961915	31.0	31.0	34.0	30.0	34.0
2	31.528315752975068	31.0	31.0	34.0	30.0	34.0
3	31.643042038027655	31.0	31.0	34.0	30.0	34.0
4	35.45044388859934	37.0	35.0	37.0	33.0	37.0
5	35.436698716201334	37.0	35.0	37.0	33.0	37.0
6	35.35736357867329	37.0	35.0	37.0	33.0	37.0
7	35.669064748201436	37.0	35.0	37.0	35.0	37.0
8	35.33224369276934	37.0	35.0	37.0	33.0	37.0
9	37.11766155429703	39.0	37.0	39.0	33.0	39.0
10	36.54013969788098	39.0	35.0	39.0	32.0	39.0
11	36.567965875859485	39.0	35.0	39.0	32.0	39.0
12	36.65934303178857	39.0	35.0	39.0	32.0	39.0
13	36.49662095119297	39.0	35.0	39.0	32.0	39.0
14	37.4853683588443	39.0	36.0	41.0	32.0	41.0
15	37.467630260750475	39.0	36.0	41.0	32.0	41.0
16	37.57327160399598	39.0	36.0	41.0	33.0	41.0
17	37.44715534029279	39.0	36.0	41.0	32.0	41.0
18	37.43029607838434	39.0	36.0	40.0	32.0	41.0
19	37.32262229016423	39.0	36.0	40.0	32.0	41.0
20	37.21405658134905	39.0	35.0	40.0	32.0	41.0
21	37.35310242257846	39.0	36.0	40.0	32.0	41.0
22	37.48749021395191	39.0	36.0	41.0	33.0	41.0
23	37.44814103249077	39.0	36.0	41.0	32.0	41.0
24	37.44638990232488	39.0	36.0	41.0	32.0	41.0
25	37.27206413105345	39.0	35.0	40.0	32.0	41.0
26	37.27983845115481	39.0	35.0	41.0	32.0	41.0
27	37.212499536593825	39.0	35.0	41.0	32.0	41.0
28	37.09196813510089	39.0	35.0	41.0	31.0	41.0
29	37.078131371834935	39.0	35.0	41.0	31.0	41.0
30	36.833127980792085	39.0	35.0	40.0	31.0	41.0
31	36.91285783134632	39.0	35.0	40.0	31.0	41.0
32	36.837219039560715	39.0	35.0	40.0	31.0	41.0
33	36.62356589417766	39.0	35.0	41.0	30.0	41.0
34	36.60514522604408	39.0	35.0	41.0	30.0	41.0
35	36.43959037074675	39.0	35.0	41.0	30.0	41.0
36	36.39174068444547	39.0	35.0	41.0	30.0	41.0
37	36.259328638937895	39.0	35.0	41.0	30.0	41.0
38	36.223891695979376	39.0	35.0	40.0	30.0	41.0
39	36.20102887075002	39.0	35.0	40.0	30.0	41.0
40	35.99843423230497	39.0	35.0	40.0	29.0	41.0
41	35.90408909610717	39.0	35.0	40.0	28.0	41.0
42	36.00816031018774	39.0	35.0	40.0	29.0	41.0
43	35.95156151526187	39.0	35.0	40.0	28.0	41.0
44	35.82976746823214	39.0	35.0	40.0	28.0	41.0
45	35.84969066274716	39.0	35.0	40.0	28.0	41.0
46	35.626283089926964	38.0	35.0	40.0	27.0	41.0
47	35.4472665577753	38.0	35.0	40.0	26.0	41.0
48	35.39165345504742	38.0	34.0	40.0	26.0	41.0
49	35.42421182787023	38.0	35.0	40.0	26.0	41.0
50	35.24073351200822	38.0	34.0	40.0	26.0	41.0
51	33.732125060787986	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	0.0
10	5.0
11	4.0
12	2.0
13	7.0
14	9.0
15	14.0
16	31.0
17	53.0
18	132.0
19	329.0
20	696.0
21	1328.0
22	2125.0
23	2640.0
24	3195.0
25	4074.0
26	4899.0
27	5538.0
28	6143.0
29	7122.0
30	8652.0
31	11354.0
32	15026.0
33	20022.0
34	30159.0
35	37035.0
36	39487.0
37	56237.0
38	96578.0
39	105628.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.98742370153589	20.053602465102788	22.85868183295134	10.10029200040998
2	27.74156546239214	35.30086509755518	22.428422827061176	14.52914661299151
3	25.438273206836172	34.54698502489309	26.10208892600984	13.912652842260897
4	23.158314815259036	21.743890125850214	39.188025148235454	15.909769910655289
5	31.243389647178894	24.441677334095136	25.46313358528091	18.85179943344506
6	21.139172323856588	38.51548648925661	26.809301270714258	13.536039916172548
7	69.47167334335018	4.757273296246301	20.56585710516158	5.205196255241941
8	67.4069534914657	13.020296100191686	12.732657160116103	6.840093248226517
9	62.83089926967187	5.977176427999765	13.07154337154708	18.12038093078129
10	38.20778478762913	23.86618138044884	21.633326863819644	16.29270696810239
11	28.543639777477807	22.06991000106856	28.62999688154902	20.756453339904617
12	23.311402408839825	18.775037563159536	33.37702072352424	24.536539304476396
13	22.812450251983922	22.318949932506253	39.06067022707993	15.807929588429893
14	19.1145779950759	30.888147923613218	28.72769380736696	21.269580273943927
15	15.033332533730517	24.689408824562054	40.64213921375782	19.635119427949608
16	16.848140160196788	25.923268660003796	29.45082551721581	27.777765662583604
17	17.498217249177316	27.27576047679589	31.024661931564175	24.201360342462618
18	18.471261184444383	26.05869230047911	31.385355492508083	24.084691022568425
19	17.979287379432616	28.622146235724365	27.933252064610812	25.465314320232206
20	20.304168911006386	27.609194850848635	32.94109180676072	19.145544431384266
21	19.385207202531397	32.529150974461416	30.031991381735473	18.053650441271717
22	17.120077808623062	23.929422694036344	31.204790638541002	27.745708858799595
23	17.954427000987874	29.752421160979676	29.81740706252821	22.475744775504243
24	21.279611654719872	25.426279164604054	27.146442894184197	26.147666286491873
25	16.73365157525389	35.13033162436404	25.31855085801017	22.817465942371896
26	18.195398213105783	25.58503666905821	29.9325498679565	26.287015249879513
27	22.504530476861312	27.031082015260782	27.595674294150612	22.86871321372729
28	16.651001720599876	25.645224953713903	34.36314906849907	23.340624257187155
29	24.617444571169376	20.957080955423596	30.668984061008242	23.756490412398787
30	21.22771016287909	26.98681309574953	33.00934881073619	18.776127930635184
31	22.33944884104841	23.305732497966464	29.430326608673656	24.92449205231147
32	24.383233637400476	25.73681582166822	30.582626956937027	19.297323583994277
33	23.262553945930858	21.40086051801178	27.774494560156665	27.5620909759007
34	24.292733136921804	20.310493042365138	29.099727190057596	26.297046630655462
35	19.63119410503728	20.727667638547544	34.280063066854794	25.361075189560385
36	23.848299353848233	21.29683946083509	30.374366769088518	24.480494416228158
37	16.97811196329387	21.69591395692176	40.28798785766779	21.03798622211658
38	20.559096826812574	22.41054080046057	31.948421256932015	25.08194111579484
39	22.538331868606356	20.787419776212978	30.679669662269575	25.994578692911084
40	23.443118799897942	20.4886590878858	33.15087850907513	22.91734360314113
41	18.2542780567907	23.295264970200257	27.115476457875832	31.33498051513321
42	18.725534879765178	21.061974306580804	36.9405597074326	23.27193110622142
43	22.887249460813283	20.538161771280155	28.884706723860077	27.68988204404648
44	21.6121737347921	21.116492680363137	30.8783346163324	26.392998968512366
45	20.093509914711454	19.709918636778966	30.57695704606366	29.619614402445908
46	27.026284398367938	23.555208576394413	27.26900019844688	22.149506826790766
47	17.7241413901313	20.43719374303528	38.450936734698324	23.38772813213509
48	20.180739312763187	21.943427373893552	30.332060511033426	27.543772802309835
49	19.561628660091024	18.11362065243228	37.51322070564221	24.81152998183448
50	21.291169549961726	17.994770597586797	32.939783365789935	27.774276486661538
51	20.267532563824663	18.252315395334538	28.394695580304475	33.08545646053633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	293.5
2	425.0
3	1746.5
4	3068.0
5	2069.0
6	1070.0
7	1048.5
8	1027.0
9	1091.5
10	1156.0
11	1212.0
12	1268.0
13	1302.5
14	1337.0
15	1266.0
16	1195.0
17	1210.5
18	1226.0
19	1170.0
20	1114.0
21	1256.5
22	1399.0
23	1451.0
24	1503.0
25	1792.5
26	2307.0
27	2532.0
28	2944.5
29	3357.0
30	4422.5
31	5488.0
32	6329.0
33	7170.0
34	8461.5
35	9753.0
36	10881.0
37	12009.0
38	12866.0
39	13723.0
40	15768.5
41	17814.0
42	20904.5
43	23995.0
44	28882.0
45	33769.0
46	64198.0
47	94627.0
48	76948.5
49	59270.0
50	55707.0
51	52144.0
52	42131.0
53	32118.0
54	26769.5
55	21421.0
56	18490.0
57	15559.0
58	13786.5
59	12014.0
60	10122.0
61	8230.0
62	7193.0
63	6156.0
64	4968.0
65	3780.0
66	3031.0
67	2282.0
68	1810.5
69	1339.0
70	1143.5
71	948.0
72	735.0
73	522.0
74	374.0
75	188.5
76	151.0
77	115.5
78	80.0
79	45.0
80	10.0
81	22.0
82	34.0
83	18.0
84	2.0
85	2.0
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.37713099055057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33065390898345	30.05682157568422
2	10.334813069088087	8.13910575968671
3	3.7032828702352463	4.374739640289461
4	2.044763434227194	3.2206767037700903
5	1.260307024183619	2.481363738979467
6	0.9292197427614304	2.1954004517833527
7	0.686490793165916	1.8922426520410862
8	0.5473479689275341	1.7242394135897052
9	0.4677072937216087	1.6575274233100523
>10	3.5800086193011746	26.121641794107394
>50	0.06935413185379852	1.9051223941566744
>100	0.03661898161880557	2.661709418349856
>500	0.0016644991644911623	0.4347699041710755
>1k	0.006103163603134262	4.943705221901054
>5k	0.0	0.0
>10k+	0.0016644991644911623	8.190933908179796
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	13428	2.928290892596623	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	12185	2.6572255381508674	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	11878	2.590276975146164	No Hit
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3945	0.8602999382852009	Illumina PCR Primer Index 5 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	2739	0.5973033031592306	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2563	0.5589223680164689	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2096	0.4570820457910725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	1988	0.43353010831710503	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	1897	0.41368542026033617	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1894	0.4130311997749481	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1637	0.35698631152671073	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1603	0.3495718126923136	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1259	0.27455453036782457	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1007	0.21960000959523376	Illumina PCR Primer Index 5 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	780	0.17009732620087623	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATG	618	0.134769419989925	No Hit
ACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	592	0.12909950911656246	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	487	0.10620179212798297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1807349512932848E-4	0.0	0.0	0.30377637871515456	0.0
2	2.1807349512932848E-4	0.0	0.0	1.9652783381055083	0.0
3	2.1807349512932848E-4	0.0	0.0	2.7062920745549666	0.0
4	2.1807349512932848E-4	0.0	0.0	4.111557677168359	0.0
5	2.1807349512932848E-4	0.0	0.0	9.083851439612179	0.0
6	2.1807349512932848E-4	0.0	0.0	10.807286271619262	0.0
7	2.1807349512932848E-4	0.0	0.0	12.618386648668334	0.0
8	2.1807349512932848E-4	0.0	0.0	14.858437590636797	0.0
9	2.1807349512932848E-4	0.0	0.0	15.589201872815176	0.0
10	2.1807349512932848E-4	0.0	0.0	19.921013780064158	0.0
11	2.1807349512932848E-4	0.0	0.0	22.194866113777664	0.0
12	2.1807349512932848E-4	0.0	0.0	26.657085971113986	0.0
13	2.1807349512932848E-4	0.0	0.0	27.524800408233585	0.0
14	2.1807349512932848E-4	0.0	0.0	27.906429024709908	0.0
15	2.1807349512932848E-4	0.0	0.0	28.703269575912476	0.0
16	2.1807349512932848E-4	0.0	0.0	29.687653332926264	0.0
17	2.1807349512932848E-4	0.0	0.0	30.92609271176572	0.0
18	2.1807349512932848E-4	0.0	0.0	32.22384808128035	0.0
19	2.1807349512932848E-4	0.0	0.0	33.459670578178255	0.0
20	2.1807349512932848E-4	0.0	0.0	34.264579848700606	0.0
21	4.3614699025865697E-4	0.0	0.0	35.14276181358642	0.0
22	4.3614699025865697E-4	0.0	0.0	36.15942044787934	0.0
23	4.3614699025865697E-4	0.0	0.0	37.097572623925714	0.0
24	6.542204853879855E-4	0.0	0.0	37.81045487950349	0.0
25	6.542204853879855E-4	0.0	0.0	38.444830676834705	0.0
26	6.542204853879855E-4	0.0	0.0	39.046059302906265	0.0
27	6.542204853879855E-4	0.0	0.0	39.73604384149546	0.0
28	6.542204853879855E-4	0.0	0.0	40.32178924941284	0.0
29	8.722939805173139E-4	0.0	0.0	40.96052651664664	0.0
30	8.722939805173139E-4	0.0	0.0	41.65945206853614	0.0
31	0.0010903674756466424	0.0	0.0	42.265042164510284	0.0
32	0.0010903674756466424	0.0	0.0	42.82570912048779	0.0
33	0.0010903674756466424	0.0	0.0	43.40425810306589	0.0
34	0.0010903674756466424	0.0	0.0	43.96666964700443	0.0
35	0.001308440970775971	0.0	0.0	44.603444252782076	0.0
36	0.001308440970775971	0.0	0.0	45.14470266769307	0.0
37	0.001308440970775971	0.0	0.0	45.70580577066083	0.0
38	0.001308440970775971	0.0	0.0	46.25164372896954	0.0
39	0.0015265144659052994	0.0	0.0	46.840224092323595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGA	25	3.887125E-5	45.000004	41
TTGGACG	25	3.887125E-5	45.000004	1
GGCGTAA	25	3.887125E-5	45.000004	29
TACGCGG	50	2.1827873E-11	45.000004	2
CCGGTGA	25	3.887125E-5	45.000004	18
AGCTAGC	25	3.887125E-5	45.000004	45
GTAACGA	25	3.887125E-5	45.000004	10
GACGTAA	20	7.0288463E-4	45.0	16
ATTGTCG	20	7.0288463E-4	45.0	1
GCTTGCG	20	7.0288463E-4	45.0	1
AGACGTA	20	7.0288463E-4	45.0	15
TCGGATT	20	7.0288463E-4	45.0	42
ACGATGA	40	6.7993824E-9	45.0	18
ATAAGCC	20	7.0288463E-4	45.0	12
CGAATAT	20	7.0288463E-4	45.0	14
ACGTAAT	20	7.0288463E-4	45.0	17
ACTCATT	20	7.0288463E-4	45.0	43
TAGTGCG	20	7.0288463E-4	45.0	1
ACGAGAC	20	7.0288463E-4	45.0	12
TCGAAGG	20	7.0288463E-4	45.0	2
>>END_MODULE