SRR2935418.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658006
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	27128	4.122758759038671	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	21932	3.3331003060762363	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	21524	3.271094792448701	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	6718	1.0209633346808389	RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	6048	0.919140554949347	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5101	0.775220894642298	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	4538	0.6896593648082237	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	3653	0.5551621109837904	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	3557	0.5405725783655468	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	3447	0.5238554055738093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	3073	0.467017018081902	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1324	0.20121397069327637	RNA PCR Primer, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1291	0.1961988188557551	RNA PCR Primer, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1140	0.1732506998416428	RNA PCR Primer, Index 16 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG	989	0.15030258082753045	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	923	0.14027227715248797	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	869	0.13206566505472594	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT	821	0.12477089874560414	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	755	0.11474059507056167	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	721	0.10957346893493372	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGC	717	0.10896557174250691	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	680	0.10334252271255884	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCACCAAC	669	0.10167080543338511	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	35	1.2108649E-7	45.000004	34
TATTGCG	35	1.2108649E-7	45.000004	1
ACGATAG	65	0.0	45.000004	1
GGTGCGT	30	2.1640808E-6	45.000004	9
CTCGATC	30	2.1640808E-6	45.000004	16
ATACCGC	60	0.0	45.000004	28
TGATCCG	20	7.031085E-4	45.0	4
ACGCCTA	20	7.031085E-4	45.0	30
GCGTTAG	20	7.031085E-4	45.0	1
CGCCTAT	20	7.031085E-4	45.0	31
GCGATTC	20	7.031085E-4	45.0	9
GAGCACG	40	6.8066583E-9	45.0	1
ATACGGA	40	6.8066583E-9	45.0	28
ACGGTCA	20	7.031085E-4	45.0	30
ACCGCCG	25	3.8889826E-5	44.999996	39
ATATGCG	25	3.8889826E-5	44.999996	1
CTCGCGG	25	3.8889826E-5	44.999996	2
CGTTTTT	3335	0.0	43.583206	1
CGATGAA	1360	0.0	43.014706	19
TACGGCT	2705	0.0	42.587803	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content