##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658006
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4964924331997	31.0	31.0	34.0	30.0	34.0
2	31.560453856043864	31.0	31.0	34.0	30.0	34.0
3	31.661819193138058	31.0	31.0	34.0	30.0	34.0
4	35.48521290079422	37.0	35.0	37.0	33.0	37.0
5	35.44821627766312	37.0	35.0	37.0	33.0	37.0
6	35.32753652702255	37.0	35.0	37.0	33.0	37.0
7	35.62280435132811	37.0	35.0	37.0	33.0	37.0
8	35.21012270404829	37.0	35.0	37.0	32.0	37.0
9	37.00435710312672	39.0	37.0	39.0	33.0	39.0
10	36.42849305325483	38.0	35.0	39.0	32.0	39.0
11	36.52510919353319	38.0	35.0	39.0	32.0	39.0
12	36.62281346978599	39.0	35.0	39.0	32.0	39.0
13	36.44956580943031	39.0	35.0	39.0	32.0	39.0
14	37.53837806950089	39.0	36.0	41.0	32.0	41.0
15	37.409585930827376	39.0	36.0	41.0	32.0	41.0
16	37.60093676957353	39.0	36.0	41.0	32.0	41.0
17	37.38732473564071	39.0	36.0	41.0	32.0	41.0
18	37.320921085825965	39.0	36.0	40.0	32.0	41.0
19	37.18421412570706	39.0	36.0	40.0	32.0	41.0
20	36.915788001933116	39.0	35.0	40.0	31.0	41.0
21	37.18089652677939	39.0	35.0	40.0	32.0	41.0
22	37.30244405066215	39.0	35.0	40.0	32.0	41.0
23	37.228798825542626	39.0	35.0	40.0	32.0	41.0
24	37.19079157332912	39.0	35.0	41.0	32.0	41.0
25	37.1110369814257	39.0	35.0	40.0	32.0	41.0
26	37.04808770740692	39.0	35.0	40.0	32.0	41.0
27	37.03879599882068	39.0	35.0	40.0	32.0	41.0
28	36.86059397634672	39.0	35.0	40.0	31.0	41.0
29	36.886311067072334	39.0	35.0	41.0	31.0	41.0
30	36.73477141545822	39.0	35.0	40.0	31.0	41.0
31	36.661194578772836	39.0	35.0	40.0	30.0	41.0
32	36.437436740698416	39.0	35.0	40.0	30.0	41.0
33	36.20487351179169	39.0	35.0	41.0	30.0	41.0
34	35.8292021653298	39.0	35.0	41.0	27.0	41.0
35	35.83768992987906	39.0	35.0	41.0	27.0	41.0
36	35.736180217201664	39.0	35.0	41.0	27.0	41.0
37	35.785343902639184	39.0	35.0	41.0	26.0	41.0
38	35.659595809156755	39.0	35.0	40.0	26.0	41.0
39	35.40865584812297	39.0	35.0	40.0	25.0	41.0
40	35.374458591563	38.0	35.0	40.0	25.0	41.0
41	34.974175007522724	38.0	34.0	40.0	23.0	41.0
42	35.22108309042775	38.0	35.0	40.0	24.0	41.0
43	35.30934976276812	39.0	35.0	40.0	24.0	41.0
44	35.277647924183064	38.0	35.0	40.0	24.0	41.0
45	35.34006528815846	38.0	35.0	40.0	24.0	41.0
46	35.09593985465178	38.0	35.0	40.0	23.0	41.0
47	34.883686470944035	38.0	34.0	40.0	23.0	41.0
48	34.86214107470145	38.0	34.0	40.0	23.0	41.0
49	34.9343151886153	38.0	34.0	40.0	23.0	41.0
50	34.77063431032544	38.0	34.0	40.0	23.0	41.0
51	33.35222171226402	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	3.0
11	5.0
12	7.0
13	7.0
14	8.0
15	26.0
16	28.0
17	98.0
18	228.0
19	577.0
20	1246.0
21	2528.0
22	3900.0
23	5009.0
24	6085.0
25	7757.0
26	9822.0
27	10740.0
28	10729.0
29	11153.0
30	13563.0
31	17095.0
32	22380.0
33	29157.0
34	42690.0
35	50095.0
36	54662.0
37	77288.0
38	131784.0
39	149292.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.91077285009559	16.697720081579803	19.388121080962787	12.003385987361817
2	28.759160250818383	38.06591429257484	19.260006747658835	13.914918708947944
3	23.335805448582537	38.09858876666778	25.489585201350746	13.076020583398936
4	21.637948590134435	19.641614210204768	43.15112628152327	15.569310918137525
5	32.88374270143433	23.005109375902347	25.445056732005483	18.666091190657834
6	19.991003121552083	39.77699291495822	27.935307580781938	12.296696382707756
7	64.66065658975754	4.440840964976004	26.17802877177412	4.720473673492339
8	62.06675926967231	15.965811861897915	15.459889423500698	6.5075394449290735
9	57.4262240769841	5.897818560924977	15.007157989440826	21.668799372650096
10	37.238870162278154	20.328082114752753	25.099923101005157	17.333124621963933
11	29.435141928797005	20.80315377063431	29.03772913924797	20.723975161320716
12	21.895089102530978	18.147250936921548	34.334033428266615	25.623626532280863
13	22.63338024273335	20.820630814916584	41.18077950656985	15.365209435780223
14	19.22277304462269	31.47934821263028	29.07146743342766	20.226411309319367
15	15.017948164606402	23.855861496703678	41.71816062467516	19.408029714014766
16	16.6024321966669	25.490041124245067	28.94654456038395	28.960982118704088
17	17.34437072002383	26.59185478551867	33.47720233554101	22.586572158916486
18	17.42598091810713	25.57149934803026	31.802293596107027	25.200226137755582
19	17.236620942666175	27.919046330884523	28.66843159484868	26.175901131600625
20	19.795868122783077	26.66571429439853	35.78599587237806	17.75242171044033
21	18.271413938474726	32.70942818150594	31.274638833080548	17.74451904693878
22	16.77203551335398	21.94356890362702	33.92081531171448	27.363580271304517
23	18.94815548794388	29.440461029230736	31.995148980404437	19.61623450242095
24	21.533694221633237	24.90433217934183	28.072844320568507	25.489129278456428
25	16.61215855174573	36.396780576468906	26.160855676088058	20.830205195697303
26	16.62902769883557	23.65145606575016	33.80212338489314	25.91739285052112
27	23.151308650680996	26.253408023635043	26.6196660820722	23.97561724361176
28	15.282687391908281	26.127421330504585	35.078403540393246	23.511487737193885
29	19.849818998610953	20.448141810257052	33.882061865697274	25.819977325434724
30	16.127208566487237	28.968276885013207	29.895168129165995	25.00934641933356
31	19.124597648045764	28.18424148108072	24.923936863797593	27.767224007075924
32	21.396461430442884	28.27785764871445	24.58579405051018	25.73988687033249
33	17.279173746136053	23.818171870773213	23.40708139439458	35.49557298869615
34	18.543143983489514	29.15672501466552	28.33773552216849	23.962395479676477
35	16.011708099926143	27.73880481332997	24.872113628143207	31.377373458600683
36	16.130400026747477	29.74015434509716	27.56205870463187	26.567386923523493
37	15.094391236554076	25.87711966152284	29.603985373993552	29.424503727929537
38	16.481308681075856	29.011741534271724	23.106628207037623	31.400321577614793
39	20.158782746661885	26.314653665772042	25.886542068005458	27.64002151956061
40	16.985711376492006	27.15370376561916	29.25216487387653	26.608419984012304
41	16.00988440834886	31.04637343732428	24.857828044121177	28.085914110205685
42	17.029176025750527	27.47771296918265	32.34165038008772	23.1514606249791
43	20.779293805831557	22.50465801223697	25.91602508183816	30.80002310009331
44	19.860761148074637	22.811342145816297	28.745938486883098	28.581958219225967
45	17.548776150977346	22.373504192970888	27.788348434512756	32.289371221539014
46	25.48077069206056	26.213742731829193	24.971504819104993	23.333981757005258
47	15.630556560274526	22.922283383434195	37.50588900405163	23.941271052239646
48	19.272772588699798	26.15006550092248	26.29231344395036	28.28484846642736
49	17.04741294152333	21.180050029938936	35.86228697002763	25.910250058510105
50	19.41228499436175	20.32169919423227	31.533147114160055	28.732868697245923
51	18.510621483694678	20.637349811399897	27.750962757178506	33.10106594772692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	405.0
2	559.0
3	4527.5
4	8496.0
5	5806.5
6	3117.0
7	3002.5
8	2888.0
9	2948.5
10	3009.0
11	3036.5
12	3064.0
13	2967.0
14	2870.0
15	2791.5
16	2713.0
17	2541.0
18	2369.0
19	2412.5
20	2456.0
21	2412.0
22	2368.0
23	2467.5
24	2567.0
25	2808.5
26	3564.5
27	4079.0
28	5058.0
29	6037.0
30	7238.0
31	8439.0
32	9385.5
33	10332.0
34	12007.0
35	13682.0
36	15017.0
37	16352.0
38	18127.5
39	19903.0
40	22498.5
41	25094.0
42	28618.0
43	32142.0
44	36808.5
45	41475.0
46	95354.5
47	149234.0
48	111442.5
49	73651.0
50	69495.5
51	65340.0
52	53243.5
53	41147.0
54	35542.0
55	29937.0
56	26728.5
57	23520.0
58	20511.0
59	17502.0
60	15354.0
61	13206.0
62	11602.5
63	9999.0
64	8560.0
65	7121.0
66	5639.0
67	4157.0
68	3210.5
69	2264.0
70	1891.0
71	1518.0
72	1260.5
73	1003.0
74	733.5
75	438.5
76	413.0
77	264.0
78	115.0
79	89.5
80	64.0
81	39.0
82	14.0
83	12.5
84	11.0
85	9.5
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	658006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.759327383769566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74065431033509	28.20934835438294
2	9.482703052319193	6.971555719665442
3	3.5822987690829686	3.9504867971758673
4	1.9407483697258772	2.853624187690823
5	1.3105009610619058	2.408656693220962
6	0.9459997065912539	2.0864587751722707
7	0.7707431191381539	1.9832399051630973
8	0.5953438946687675	1.7507552904046118
9	0.5209121667644904	1.72335427886562
>10	4.002597547262954	26.38670474524241
>50	0.06987376699115917	1.795831111312347
>100	0.027287979998270557	1.8487211943035604
>500	0.004547996666378426	1.2410931661199336
>1k	0.00330763393918431	3.3471215769604825
>5k	0.0012403627271941162	2.7154802350067175
>10k+	0.0012403627271941162	10.72756796931293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	27128	4.122758759038671	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	21932	3.3331003060762363	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	21524	3.271094792448701	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	6718	1.0209633346808389	RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	6048	0.919140554949347	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5101	0.775220894642298	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	4538	0.6896593648082237	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	3653	0.5551621109837904	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	3557	0.5405725783655468	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	3447	0.5238554055738093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	3073	0.467017018081902	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1324	0.20121397069327637	RNA PCR Primer, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1291	0.1961988188557551	RNA PCR Primer, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1140	0.1732506998416428	RNA PCR Primer, Index 16 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG	989	0.15030258082753045	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	923	0.14027227715248797	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	869	0.13206566505472594	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT	821	0.12477089874560414	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	755	0.11474059507056167	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	721	0.10957346893493372	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGC	717	0.10896557174250691	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	680	0.10334252271255884	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCACCAAC	669	0.10167080543338511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.59871490533521E-4	0.0	0.0	0.22401011540928198	0.0
2	7.59871490533521E-4	0.0	0.0	1.5794688802229766	0.0
3	7.59871490533521E-4	0.0	0.0	2.0913183162463564	0.0
4	7.59871490533521E-4	0.0	0.0	3.7017899532831007	0.0
5	7.59871490533521E-4	0.0	0.0	9.32879031498193	0.0
6	7.59871490533521E-4	0.0	0.0	10.939261952018674	0.0
7	7.59871490533521E-4	0.0	0.0	12.517515037856798	0.0
8	7.59871490533521E-4	0.0	0.0	14.233456837779595	0.0
9	7.59871490533521E-4	0.0	0.0	14.734668072935506	0.0
10	7.59871490533521E-4	0.0	0.0	19.260614644851263	0.0
11	7.59871490533521E-4	0.0	0.0	20.612122077914183	0.0
12	7.59871490533521E-4	0.0	0.0	25.197186651793448	0.0
13	7.59871490533521E-4	0.0	0.0	25.857211028470864	0.0
14	7.59871490533521E-4	0.0	0.0	26.19444199596964	0.0
15	9.118457886402252E-4	0.0	0.0	27.00294526189731	0.0
16	9.118457886402252E-4	0.0	0.0	27.765400315498642	0.0
17	9.118457886402252E-4	0.0	0.0	28.622079433926135	0.0
18	9.118457886402252E-4	0.0	0.0	29.482557909806292	0.0
19	9.118457886402252E-4	0.0	0.0	30.657927131363543	0.0
20	9.118457886402252E-4	0.0	0.0	31.26628024668468	0.0
21	9.118457886402252E-4	0.0	0.0	31.897885429616142	0.0
22	9.118457886402252E-4	0.0	0.0	32.69179916292557	0.0
23	9.118457886402252E-4	0.0	0.0	33.40015136640091	0.0
24	9.118457886402252E-4	0.0	0.0	33.94361145643049	0.0
25	9.118457886402252E-4	0.0	0.0	34.470506347966435	0.0
26	9.118457886402252E-4	0.0	0.0	34.97840445223904	0.0
27	9.118457886402252E-4	0.0	0.0	35.56593708871956	0.0
28	0.0010638200867469293	0.0	0.0	36.08204180508992	0.0
29	0.0010638200867469293	0.0	0.0	36.58842016638146	0.0
30	0.0010638200867469293	0.0	0.0	37.17276134260174	0.0
31	0.0010638200867469293	0.0	0.0	37.707406923341125	0.0
32	0.0010638200867469293	0.0	0.0	38.21287343884402	0.0
33	0.0010638200867469293	0.0	0.0	38.7336893584557	0.0
34	0.0012157943848536336	0.0	0.0	39.21772749792555	0.0
35	0.0012157943848536336	0.0	0.0	39.73292036850728	0.0
36	0.0012157943848536336	0.0	0.0	40.21361507341879	0.0
37	0.0012157943848536336	0.0	0.0	40.69734926429242	0.0
38	0.0012157943848536336	0.0	0.0	41.162542590797045	0.0
39	0.0012157943848536336	0.0	0.0	41.63579055510132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	35	1.2108649E-7	45.000004	34
TATTGCG	35	1.2108649E-7	45.000004	1
ACGATAG	65	0.0	45.000004	1
GGTGCGT	30	2.1640808E-6	45.000004	9
CTCGATC	30	2.1640808E-6	45.000004	16
ATACCGC	60	0.0	45.000004	28
TGATCCG	20	7.031085E-4	45.0	4
ACGCCTA	20	7.031085E-4	45.0	30
GCGTTAG	20	7.031085E-4	45.0	1
CGCCTAT	20	7.031085E-4	45.0	31
GCGATTC	20	7.031085E-4	45.0	9
GAGCACG	40	6.8066583E-9	45.0	1
ATACGGA	40	6.8066583E-9	45.0	28
ACGGTCA	20	7.031085E-4	45.0	30
ACCGCCG	25	3.8889826E-5	44.999996	39
ATATGCG	25	3.8889826E-5	44.999996	1
CTCGCGG	25	3.8889826E-5	44.999996	2
CGTTTTT	3335	0.0	43.583206	1
CGATGAA	1360	0.0	43.014706	19
TACGGCT	2705	0.0	42.587803	7
>>END_MODULE