FastQCFastQC Report
Sat 14 Jan 2017
SRR2935417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550674
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109661.9913778387939143No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC87131.58224285148745No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG39100.7100389704253333No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC35030.636129543069039No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT27520.49975121396688427No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC14740.267671980155228TruSeq Adapter, Index 21 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT14020.25459709374330364No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT11770.21373807370604023No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG8990.16325448450444363No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT8930.1621649106367833No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC8020.14563970697726786No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC7740.1405550289281862No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC7190.1305672684746329No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT6510.11821876464114885No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT6280.11404206481511747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56050.044.036571
TATGGGC950.040.2631574
CGGTCTA451.9252184E-840.00000431
TAGCGCG403.4538425E-739.3750041
GTTTTTT64250.039.221792
CCGAACC750.039.018
CGGGTAT356.2423605E-638.571436
GCGATAT356.2423605E-638.571439
TAGTGCG356.2423605E-638.571431
GCGTAAG356.2423605E-638.571431
GCGCGAC900.037.5000049
TAACGTC301.1391499E-437.49999632
GCCGATT601.546141E-1037.4999969
AGTTGCG301.1391499E-437.4999961
CCGATGA2850.037.10526318
TACGGCT4500.037.07
CGTAAGG552.743036E-936.818182
TCCGAAC800.036.56250417
GGGCGAT7450.036.5436257
GAATCTG12200.036.5163961
Produced by FastQC (version 0.11.5)