##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	550674
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.924423524626185	33.0	31.0	34.0	30.0	34.0
2	31.983420317647102	33.0	31.0	34.0	30.0	34.0
3	32.03270174368138	33.0	31.0	34.0	30.0	34.0
4	35.75319699132336	37.0	35.0	37.0	35.0	37.0
5	35.75733192415113	37.0	35.0	37.0	35.0	37.0
6	35.68233292292717	37.0	35.0	37.0	33.0	37.0
7	35.94837962206314	37.0	35.0	37.0	35.0	37.0
8	35.74750578382128	37.0	35.0	37.0	35.0	37.0
9	37.589986089773625	39.0	37.0	39.0	35.0	39.0
10	37.07987121236884	39.0	37.0	39.0	33.0	39.0
11	37.013646912692444	39.0	37.0	39.0	33.0	39.0
12	37.025131021257586	39.0	37.0	39.0	33.0	39.0
13	36.973136556292836	39.0	37.0	39.0	33.0	39.0
14	38.043853532216886	40.0	37.0	41.0	33.0	41.0
15	38.12141847989918	40.0	37.0	41.0	33.0	41.0
16	38.08914167002619	40.0	37.0	41.0	33.0	41.0
17	38.044345656413775	40.0	37.0	41.0	33.0	41.0
18	37.82411372245648	39.0	37.0	41.0	33.0	41.0
19	37.5736769849312	39.0	37.0	41.0	33.0	41.0
20	37.42297620733864	39.0	35.0	41.0	33.0	41.0
21	37.429615344105585	39.0	35.0	41.0	33.0	41.0
22	37.541058775246334	39.0	35.0	41.0	33.0	41.0
23	37.54784863639831	39.0	35.0	41.0	33.0	41.0
24	37.50534617577732	39.0	35.0	41.0	33.0	41.0
25	37.29314984909402	39.0	35.0	41.0	33.0	41.0
26	37.31623973530619	39.0	35.0	41.0	33.0	41.0
27	37.335559695936254	39.0	35.0	41.0	33.0	41.0
28	37.19733090721552	39.0	35.0	41.0	33.0	41.0
29	37.11365889800499	39.0	35.0	41.0	33.0	41.0
30	36.92520983376735	39.0	35.0	41.0	32.0	41.0
31	36.79824723883822	39.0	35.0	41.0	32.0	41.0
32	36.51749310844529	39.0	35.0	41.0	31.0	41.0
33	36.17176587236732	39.0	35.0	41.0	30.0	41.0
34	35.806569767230705	39.0	35.0	41.0	27.0	41.0
35	35.4926417444804	39.0	35.0	41.0	25.0	41.0
36	35.37536182932188	39.0	35.0	41.0	24.0	41.0
37	35.32979766613278	39.0	35.0	41.0	23.0	41.0
38	35.20712980819868	39.0	35.0	41.0	23.0	41.0
39	35.10558333968918	39.0	35.0	41.0	23.0	41.0
40	34.91126873613063	38.0	35.0	41.0	22.0	41.0
41	34.759990484388226	38.0	34.0	40.0	21.0	41.0
42	34.74632359617487	38.0	35.0	40.0	21.0	41.0
43	34.709363434627384	38.0	34.0	40.0	21.0	41.0
44	34.590320225759704	38.0	34.0	40.0	20.0	41.0
45	34.5810080011041	38.0	34.0	40.0	20.0	41.0
46	34.48419754700603	38.0	34.0	40.0	20.0	41.0
47	34.418750476688565	38.0	34.0	40.0	20.0	41.0
48	34.34961883074196	38.0	34.0	40.0	20.0	41.0
49	34.29870667581909	38.0	34.0	40.0	20.0	41.0
50	34.19836781834624	37.0	34.0	40.0	20.0	41.0
51	33.057524052343126	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	7.0
11	8.0
12	9.0
13	16.0
14	21.0
15	39.0
16	62.0
17	103.0
18	206.0
19	369.0
20	748.0
21	1292.0
22	1897.0
23	2661.0
24	3936.0
25	6619.0
26	10333.0
27	12543.0
28	12867.0
29	11705.0
30	11431.0
31	12890.0
32	15792.0
33	21310.0
34	34534.0
35	37655.0
36	43133.0
37	60027.0
38	104635.0
39	143765.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.41206594101047	18.71506553786814	21.017516715879086	20.855351805242304
2	37.623893628535214	26.23330682036922	21.532703559637824	14.610095991457742
3	27.368824386115925	26.00885460363119	32.575897899664774	14.046423110588115
4	24.832114826557998	21.766961941184803	36.849025012984086	16.55189821927311
5	25.2617701217054	25.5759305868808	31.605813966157836	17.55648532525596
6	23.353744683787504	31.860229464256527	31.479968184443063	13.306057667512903
7	70.32890603151773	4.965369710572862	20.26625553412727	4.439468723782128
8	71.08652306083091	6.690891525657648	17.734449056973816	4.488136356537624
9	65.21099597947243	6.4395631535173266	18.98981974816316	9.359621118847086
10	36.452783316444936	25.29318616822294	24.936169130919563	13.317861384412558
11	27.44182583524917	21.376712900917784	33.205308403883244	17.976152859949806
12	24.145138502998144	19.05319662813207	36.73897805235039	20.062686816519392
13	22.988374246832063	19.283459905497626	39.392635207037195	18.335530640633117
14	18.646603979850145	24.572069863476393	37.20967396317967	19.57165219349379
15	17.941649687473895	23.19394051653065	39.93033991072758	18.93406988526787
16	21.584095127062472	23.571114670385747	35.01926729789312	19.825522904658655
17	21.320055059799444	24.368864337157735	34.85437845258719	19.456702150455623
18	21.45062232827408	23.73219000715487	34.72580873620327	20.09137892836778
19	20.928898041309377	25.49947882049997	32.576624282243216	20.99499885594744
20	22.662773256046226	25.298270846272025	34.36552297729691	17.673432920384837
21	22.252730290516713	25.710492959536857	35.07483556514381	16.961941184802622
22	21.171328226863807	21.518539099358243	36.34618667305883	20.96394600071912
23	21.068726687659122	23.74108819374076	35.86132630194997	19.32885881665014
24	22.24909837762451	23.43746027595274	34.18338254575302	20.130058800669726
25	21.316967933841074	26.579246523351387	31.929962191786792	20.17382335102075
26	20.201970675935307	24.293683740289175	33.15736715370618	22.346978430069335
27	21.654735832815785	24.089207044458245	33.08254974812684	21.173507374599126
28	19.28709181838983	25.347664861605963	33.94694501647073	21.418298303533486
29	21.03077319793562	25.708495407446147	32.58370651238301	20.677024882235223
30	21.922952599904843	26.372772275429746	32.590243955588974	19.11403116907644
31	24.100465974424072	23.690241413249943	31.004005999920096	21.20528661240589
32	24.70681383177706	24.480545658592927	30.56018624449311	20.252454265136905
33	25.19893802867032	23.380076052255962	30.333191688730537	21.087794230343178
34	24.293865335933784	24.811412923072453	31.443285864231836	19.451435876761934
35	22.290320588950998	25.643847357964965	31.81065385327798	20.255178199806057
36	23.47014749198255	26.61320490889347	31.384993662312006	18.531653936811978
37	22.33444833059124	26.184820783258335	31.326883056036785	20.15384783011364
38	23.598535612721864	25.13610593563524	29.851418443580048	21.413940008062845
39	23.871655462215394	23.811728899494074	30.101657241852713	22.21495839643782
40	23.39387732124633	23.905250656468255	32.71318420698998	19.987687815295438
41	21.237610637146478	26.033006824364325	31.615801726611387	21.113580811877807
42	20.893486890610415	25.60280674228309	32.873896352469885	20.62981001463661
43	22.21532158772704	24.575701776368593	31.259692667531063	21.949283968373305
44	22.43051242658996	24.52267584814246	30.103473198298815	22.943338526968766
45	21.472050614338066	23.70349789530648	30.199537294297535	24.62491419605792
46	23.304532264098178	24.612020905290606	30.027021431917976	22.05642539869324
47	20.716431137115606	24.52194946556402	33.543076302857955	21.218543094462422
48	20.99445406901361	24.408996974616564	31.61507534403295	22.98147361233688
49	20.918365493921996	23.484675143551357	33.74337629886285	21.8535830636638
50	20.56970185627068	23.24260814928615	33.55415363717917	22.63353635726401
51	20.252454265136905	23.268576326465386	31.901814866872236	24.577154541525477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	250.0
1	518.5
2	787.0
3	9914.0
4	19041.0
5	12679.5
6	6318.0
7	5998.5
8	5679.0
9	5569.5
10	5460.0
11	5234.0
12	5008.0
13	4828.0
14	4648.0
15	4299.0
16	3950.0
17	3688.0
18	3426.0
19	3198.5
20	2971.0
21	2978.5
22	2986.0
23	3026.5
24	3067.0
25	3373.0
26	4247.0
27	4815.0
28	5682.0
29	6549.0
30	7427.5
31	8306.0
32	9477.0
33	10648.0
34	12199.5
35	13751.0
36	14856.0
37	15961.0
38	17110.0
39	18259.0
40	19517.5
41	20776.0
42	22084.5
43	23393.0
44	25920.0
45	28447.0
46	43909.5
47	59372.0
48	51172.0
49	42972.0
50	42705.5
51	42439.0
52	37883.5
53	33328.0
54	30860.5
55	28393.0
56	27331.0
57	26269.0
58	24910.0
59	23551.0
60	22475.0
61	21399.0
62	19586.0
63	17773.0
64	15209.5
65	12646.0
66	10647.5
67	8649.0
68	7345.5
69	6042.0
70	4987.5
71	3933.0
72	3178.0
73	2423.0
74	2072.5
75	1243.0
76	764.0
77	579.0
78	394.0
79	301.5
80	209.0
81	159.0
82	109.0
83	85.5
84	62.0
85	42.5
86	23.0
87	20.0
88	17.0
89	9.5
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	550674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.74076771580481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83323321934772	28.04318765717264
2	9.59442457974584	7.817684463404667
3	5.278587694456624	6.451611453820888
4	3.7738888741067083	6.150045200209661
5	2.8729771242369417	5.852364683567906
6	2.2178284868563165	5.421362112990482
7	1.7267869323283007	4.924543771327211
8	1.3710459895238352	4.468597294950105
9	1.0226779350514195	3.7498215780007906
>10	3.2553269895064636	17.697369580364803
>50	0.027729200349297458	0.7570871809093306
>100	0.0183370518265039	1.4194245812069124
>500	0.0035779613320007612	1.0704902971233132
>1k	0.0026834709990005714	2.5906776245956205
>5k	4.4724516650009516E-4	1.5876054398017752
>10k+	4.4724516650009516E-4	1.9981270805539155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10966	1.9913778387939143	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	8713	1.58224285148745	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	3910	0.7100389704253333	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	3503	0.636129543069039	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	2752	0.49975121396688427	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	1474	0.267671980155228	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1402	0.25459709374330364	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1177	0.21373807370604023	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	899	0.16325448450444363	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	893	0.1621649106367833	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	802	0.14563970697726786	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	774	0.1405550289281862	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	719	0.1305672684746329	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	651	0.11821876464114885	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	628	0.11404206481511747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.44786933830179E-4	0.0	0.0	0.05883698885365933	0.0
2	5.44786933830179E-4	0.0	0.0	0.46397687197870247	0.0
3	5.44786933830179E-4	0.0	0.0	0.7754134024849548	0.0
4	5.44786933830179E-4	0.0	0.0	1.6027631593283866	0.0
5	7.263825784402387E-4	0.0	0.0	3.898495298488761	0.0
6	7.263825784402387E-4	0.0	0.0	5.185463631840254	0.0
7	7.263825784402387E-4	0.0	0.0	6.08726760297381	0.0
8	7.263825784402387E-4	0.0	0.0	7.104929595368585	0.0
9	9.079782230502983E-4	0.0	0.0	7.72852903895953	0.0
10	9.079782230502983E-4	0.0	0.0	9.203085673193215	0.0
11	0.001089573867660358	0.0	0.0	9.882071788390228	0.0
12	0.001089573867660358	0.0	0.0	11.067346560760086	0.0
13	0.001089573867660358	0.0	0.0	11.418370941791332	0.0
14	0.001089573867660358	0.0	0.0	11.665885805394844	0.0
15	0.001089573867660358	0.0	0.0	11.921754068650417	0.0
16	0.001089573867660358	0.0	0.0	12.155467663263565	0.0
17	0.001089573867660358	0.0	0.0	12.410791139585308	0.0
18	0.001089573867660358	0.0	0.0	12.685182158591108	0.0
19	0.001089573867660358	0.0	0.0	12.987357311222247	0.0
20	0.001089573867660358	0.0	0.0	13.220162927612344	0.0
21	0.0012711695122704177	0.0	0.0	13.441164827102787	0.0
22	0.0012711695122704177	0.0	0.0	13.715555846108588	0.0
23	0.0012711695122704177	0.0	0.0	13.962525922778267	0.0
24	0.0012711695122704177	0.0	0.0	14.190610052408504	0.0
25	0.0012711695122704177	0.0	0.0	14.39436036566099	0.0
26	0.0012711695122704177	0.0	0.0	14.589575683616804	0.0
27	0.0014527651568804773	0.0	0.0	14.825468425965271	0.0
28	0.0014527651568804773	0.0	0.0	15.056276490264658	0.0
29	0.0014527651568804773	0.0	0.0	15.27509924201978	0.0
30	0.0014527651568804773	0.0	0.0	15.58145109447695	0.0
31	0.001634360801490537	0.0	0.0	15.836956166443304	0.0
32	0.001634360801490537	0.0	0.0	16.09082687760817	0.0
33	0.001634360801490537	0.0	0.0	16.323269302709043	0.0
34	0.001634360801490537	0.0	0.0	16.571147357601774	0.0
35	0.001634360801490537	0.0	0.0	16.846809546119847	0.0
36	0.001634360801490537	0.0	0.0	17.110668017738263	0.0
37	0.001634360801490537	0.0	0.0	17.37398170242285	0.0
38	0.001634360801490537	0.0	0.0	17.722100553140333	0.0
39	0.001634360801490537	0.0	0.0	18.173547325640943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5605	0.0	44.03657	1
TATGGGC	95	0.0	40.263157	4
CGGTCTA	45	1.9252184E-8	40.000004	31
TAGCGCG	40	3.4538425E-7	39.375004	1
GTTTTTT	6425	0.0	39.22179	2
CCGAACC	75	0.0	39.0	18
CGGGTAT	35	6.2423605E-6	38.57143	6
GCGATAT	35	6.2423605E-6	38.57143	9
TAGTGCG	35	6.2423605E-6	38.57143	1
GCGTAAG	35	6.2423605E-6	38.57143	1
GCGCGAC	90	0.0	37.500004	9
TAACGTC	30	1.1391499E-4	37.499996	32
GCCGATT	60	1.546141E-10	37.499996	9
AGTTGCG	30	1.1391499E-4	37.499996	1
CCGATGA	285	0.0	37.105263	18
TACGGCT	450	0.0	37.0	7
CGTAAGG	55	2.743036E-9	36.81818	2
TCCGAAC	80	0.0	36.562504	17
GGGCGAT	745	0.0	36.543625	7
GAATCTG	1220	0.0	36.516396	1
>>END_MODULE