SRR2935414.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935414.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560582
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3439	0.613469572694093	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	1774	0.31645682522806656	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	1417	0.25277301090652216	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	1408	0.25116753659589497	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1008	0.179813122790243	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	829	0.14788202261221373	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	710	0.12665408450503227	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	599	0.10685323467396383	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCAT	30	2.1635042E-6	45.000004	9
AACCGAT	20	7.030192E-4	45.0	16
ATTAGCG	20	7.030192E-4	45.0	1
CGTTTTT	1765	0.0	41.81303	1
CGTAAGG	60	3.6379788E-12	41.250004	2
ACGTAAG	40	3.453988E-7	39.375	1
TGCGACG	35	6.2425624E-6	38.57143	1
ATAGCGG	130	0.0	38.076927	2
CCCTAGC	210	0.0	37.500004	21
TCGACAC	30	1.1391759E-4	37.500004	37
AATCGAG	30	1.1391759E-4	37.500004	1
TAGTACG	30	1.1391759E-4	37.500004	1
TAGGGCG	145	0.0	37.24138	5
TAGGGAC	505	0.0	36.980198	5
CGAGGGT	80	0.0	36.5625	4
TACAGCG	75	1.8189894E-12	36.000004	1
GTACGAG	75	1.8189894E-12	36.000004	1
CTCACGA	25	0.00210619	36.0	24
AAATCGG	25	0.00210619	36.0	2
ACACGGT	25	0.00210619	36.0	32