##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935414.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560582
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936291211633623	33.0	31.0	34.0	30.0	34.0
2	31.986740566054564	33.0	31.0	34.0	30.0	34.0
3	32.05258106753338	33.0	31.0	34.0	30.0	34.0
4	35.771394729049454	37.0	35.0	37.0	35.0	37.0
5	35.78722648961258	37.0	35.0	37.0	35.0	37.0
6	35.74888241149377	37.0	35.0	37.0	35.0	37.0
7	36.0282902412136	37.0	35.0	37.0	35.0	37.0
8	35.90326838892437	37.0	35.0	37.0	35.0	37.0
9	37.72554059887759	39.0	38.0	39.0	35.0	39.0
10	37.05055281832096	39.0	37.0	39.0	33.0	39.0
11	36.97560570978019	39.0	37.0	39.0	33.0	39.0
12	37.00994145370347	39.0	37.0	39.0	33.0	39.0
13	36.953113014688306	39.0	37.0	39.0	33.0	39.0
14	37.934578705702286	40.0	37.0	41.0	33.0	41.0
15	38.10161046911959	40.0	37.0	41.0	33.0	41.0
16	38.047727183534256	40.0	37.0	41.0	33.0	41.0
17	38.08828324848104	40.0	37.0	41.0	33.0	41.0
18	38.010050269184525	40.0	37.0	41.0	33.0	41.0
19	37.8841115126779	40.0	37.0	41.0	33.0	41.0
20	37.907101904806076	40.0	36.0	41.0	33.0	41.0
21	37.84944397073042	40.0	36.0	41.0	33.0	41.0
22	37.964513666154104	40.0	36.0	41.0	34.0	41.0
23	37.994013364681706	40.0	36.0	41.0	34.0	41.0
24	37.96881812116693	40.0	36.0	41.0	34.0	41.0
25	37.730030575366314	39.0	36.0	41.0	33.0	41.0
26	37.81625703286941	40.0	36.0	41.0	34.0	41.0
27	37.82829630633877	40.0	36.0	41.0	34.0	41.0
28	37.718865393466075	40.0	36.0	41.0	33.0	41.0
29	37.68429061225655	40.0	36.0	41.0	33.0	41.0
30	37.545798830501155	40.0	36.0	41.0	33.0	41.0
31	37.52969770702591	40.0	36.0	41.0	33.0	41.0
32	37.48997648872065	40.0	36.0	41.0	33.0	41.0
33	37.37345294711567	40.0	36.0	41.0	33.0	41.0
34	37.29775126564891	40.0	36.0	41.0	33.0	41.0
35	37.218000221198686	40.0	36.0	41.0	32.0	41.0
36	37.13012369287633	40.0	35.0	41.0	32.0	41.0
37	37.06237981240925	40.0	35.0	41.0	32.0	41.0
38	36.977987163340956	40.0	35.0	41.0	31.0	41.0
39	36.920536157065335	40.0	35.0	41.0	31.0	41.0
40	36.82810900100253	40.0	35.0	41.0	31.0	41.0
41	36.68352355230814	39.0	35.0	41.0	31.0	41.0
42	36.71289481289089	39.0	35.0	41.0	31.0	41.0
43	36.64916461820037	39.0	35.0	41.0	31.0	41.0
44	36.538658037539555	39.0	35.0	41.0	31.0	41.0
45	36.51320948585577	39.0	35.0	41.0	31.0	41.0
46	36.44853919676336	39.0	35.0	41.0	31.0	41.0
47	36.38983413666511	39.0	35.0	41.0	31.0	41.0
48	36.29999001038207	39.0	35.0	41.0	30.0	41.0
49	36.21520847975854	39.0	35.0	41.0	30.0	41.0
50	36.100508043426295	39.0	35.0	41.0	30.0	41.0
51	34.92467114534537	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	4.0
10	5.0
11	4.0
12	11.0
13	16.0
14	15.0
15	29.0
16	37.0
17	84.0
18	140.0
19	279.0
20	494.0
21	726.0
22	1076.0
23	1653.0
24	2408.0
25	3627.0
26	5105.0
27	5996.0
28	6534.0
29	7242.0
30	8354.0
31	10469.0
32	13637.0
33	19865.0
34	34933.0
35	39010.0
36	40846.0
37	61391.0
38	115520.0
39	180997.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04391864169738	21.181200966138764	25.670285524686843	13.104594867477015
2	32.58060372969521	24.7052170779654	26.105369062866806	16.608810129472584
3	30.757676842995313	24.54395610276463	28.90513787456608	15.79322917967398
4	27.312150586354893	25.22610429874666	29.162905694438994	18.29883942045945
5	25.137446439593138	29.38588823758166	27.174436567709986	18.30222875511522
6	25.475487974997414	34.09456600461663	26.193313377882273	14.236632642503682
7	80.23001095290252	5.431141206817201	10.11841264971048	4.2204351905698
8	82.70226300523385	4.8328344470568085	8.744661797917166	3.72024074979218
9	76.81142098747374	6.724261571010129	10.47393601649714	5.990381425018998
10	38.71565622870517	31.440716969149918	17.161271678362844	12.682355123782068
11	28.124163815463216	25.153857954768437	28.356600818435123	18.365377411333224
12	25.905041546107437	22.040486494393328	32.25665469101755	19.797817268481683
13	24.947643698870102	23.05336239836456	33.244021392053256	18.754972510712083
14	20.46266201911585	26.891516316970577	32.59112850573154	20.054693158182033
15	19.384853598581476	25.610347817090094	35.82294829302404	19.181850291304396
16	23.459369012918714	25.849385103339028	30.86024881284094	19.83099707090131
17	23.714639428308438	25.20202218408725	29.82971269145281	21.2536256961515
18	24.26371164254293	24.5386045217292	30.899315354399537	20.298368481328332
19	24.060708335265847	26.65676029554998	28.970783935267274	20.311747433916892
20	23.7066120567553	27.77452718781552	29.089589034253688	19.429271721175493
21	23.750851793314805	26.535101020011346	31.19008459065757	18.523962596016283
22	22.42027036187391	24.16274514700793	31.090366797364165	22.32661769375399
23	21.28858935891627	26.23041767306121	30.951939234581204	21.529053733441316
24	21.413637969110674	25.90736056455612	31.004384728728358	21.674616737604847
25	22.43115190997927	27.626823551237823	28.887834429218206	21.0541901095647
26	21.189049951657385	26.68512367503773	29.290273323082083	22.835553050222803
27	20.907378403159573	26.645165203306565	30.305825017571024	22.141631375962838
28	20.31406645236558	26.42699908309578	30.95086891837412	22.30806554616452
29	21.278242968914448	25.721303930557887	31.065571138566707	21.93488196196096
30	22.186763042694913	26.543485163633505	30.754287508339544	20.51546428533203
31	23.78849124659729	26.68994009796961	28.747979778159127	20.773588877273976
32	24.220363836155993	26.757905177119497	28.138791470293373	20.882939516431136
33	23.405318044460934	26.1153586807996	28.645764580382533	21.833558694356935
34	23.060319453710605	25.440167540163618	30.462091183805402	21.037421822320372
35	22.004630901455986	27.420787681373998	28.99879054268599	21.575790874484017
36	23.689843769510972	28.095265277871928	28.45292927707276	19.761961675544345
37	22.60918117242437	28.62721243279306	27.991444605784704	20.772161788997863
38	22.64575030949977	27.471984473279555	28.24439600272574	21.637869214494938
39	22.500722463439782	26.212400683575282	28.45061025862407	22.83626659436086
40	24.206984883567433	24.42229682722599	30.181846723583703	21.188871565622872
41	22.359975882208133	26.440199649649827	28.2047943030636	22.995030165078436
42	21.951115091101748	26.680842410209387	29.467945813458158	21.900096685230707
43	21.59362947793543	26.26110007099764	29.750509292128534	22.39476115893839
44	22.182481777866574	25.71327655900475	28.946701820607867	23.15753984252081
45	21.217234945110615	25.404311947226276	29.08245359287312	24.295999514789987
46	22.562087259312644	25.63871119657784	29.103146372876758	22.696055171232754
47	20.99585787627858	26.21311422771334	30.734843430577506	22.05618446543057
48	21.305714418229627	26.583443635364674	29.46081037207759	22.650031574328107
49	22.402431758422498	25.421437006539634	30.33097744843752	21.845153786600356
50	21.093613423192327	25.478520537584153	30.14402888426671	23.283837154956814
51	21.437898469804594	25.094455405275234	28.795965621443433	24.671680503476743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	442.0
2	672.0
3	3205.5
4	5739.0
5	3835.5
6	1932.0
7	1898.0
8	1864.0
9	1806.0
10	1748.0
11	1793.0
12	1838.0
13	1780.5
14	1723.0
15	1730.0
16	1737.0
17	1723.0
18	1709.0
19	1641.0
20	1573.0
21	1810.5
22	2048.0
23	2383.0
24	2718.0
25	3174.0
26	4833.5
27	6037.0
28	6990.5
29	7944.0
30	9458.0
31	10972.0
32	11843.5
33	12715.0
34	14963.0
35	17211.0
36	18853.5
37	20496.0
38	21959.5
39	23423.0
40	25287.5
41	27152.0
42	30272.5
43	33393.0
44	35933.0
45	38473.0
46	45085.0
47	51697.0
48	51870.5
49	52044.0
50	50489.0
51	48934.0
52	43884.0
53	38834.0
54	34883.5
55	30933.0
56	29147.0
57	27361.0
58	25279.5
59	23198.0
60	20719.0
61	18240.0
62	16059.0
63	13878.0
64	11819.0
65	9760.0
66	8397.5
67	7035.0
68	5680.5
69	4326.0
70	3734.5
71	3143.0
72	2519.0
73	1895.0
74	1503.5
75	817.5
76	523.0
77	432.0
78	341.0
79	260.5
80	180.0
81	127.5
82	75.0
83	61.5
84	48.0
85	37.5
86	27.0
87	20.0
88	13.0
89	8.5
90	4.0
91	5.5
92	7.0
93	11.0
94	15.0
95	7.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.60114592943061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19312751063538	24.593729585162816
2	8.328567239189988	5.596988063741955
3	3.1927480364896934	3.2183997806997966
4	1.8925168110513282	2.5436293416814535
5	1.3974324284738735	2.3477665477834613
6	1.186229667220628	2.391520569246014
7	1.0088746013091379	2.372953989215912
8	0.8654467284081826	2.3264001452313314
9	0.8244957275693178	2.493360113422384
>10	8.043911347715278	47.782600276454154
>50	0.04212273802429537	0.9787567969959529
>100	0.02026157018889938	1.3501576463776117
>500	0.001599597646492056	0.38304631740383943
>1k	0.00266599607748676	1.6206908265833166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3439	0.613469572694093	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	1774	0.31645682522806656	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	1417	0.25277301090652216	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	1408	0.25116753659589497	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	1008	0.179813122790243	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	829	0.14788202261221373	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTG	710	0.12665408450503227	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	599	0.10685323467396383	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.351581035423898E-4	0.0	0.0	0.11630769450321274	0.0
2	5.351581035423898E-4	0.0	0.0	0.5588834461327692	0.0
3	5.351581035423898E-4	0.0	0.0	0.8575016679094227	0.0
4	5.351581035423898E-4	0.0	0.0	1.2251552850430445	0.0
5	5.351581035423898E-4	0.0	0.0	2.277276116607383	0.0
6	5.351581035423898E-4	0.0	0.0	3.0081237000117733	0.0
7	7.135441380565199E-4	0.0	0.0	3.656913707539664	0.0
8	7.135441380565199E-4	0.0	0.0	4.575958557356461	0.0
9	7.135441380565199E-4	0.0	0.0	4.994630580361124	0.0
10	7.135441380565199E-4	0.0	0.0	5.998587182606648	0.0
11	7.135441380565199E-4	0.0	0.0	6.907820800525169	0.0
12	7.135441380565199E-4	0.0	0.0	7.88965753449094	0.0
13	7.135441380565199E-4	0.0	0.0	8.245537673346629	0.0
14	7.135441380565199E-4	0.0	0.0	8.417145038549222	0.0
15	7.135441380565199E-4	0.0	0.0	8.647619795141479	0.0
16	7.135441380565199E-4	0.0	0.0	9.054339953833693	0.0
17	7.135441380565199E-4	0.0	0.0	9.569697207545016	0.0
18	7.135441380565199E-4	0.0	0.0	10.176031338858543	0.0
19	7.135441380565199E-4	0.0	0.0	10.573118651686997	0.0
20	7.135441380565199E-4	0.0	0.0	10.937918092268392	0.0
21	7.135441380565199E-4	0.0	0.0	11.363725556653621	0.0
22	8.919301725706498E-4	0.0	0.0	11.830918581046127	0.0
23	8.919301725706498E-4	0.0	0.0	12.301144168025374	0.0
24	8.919301725706498E-4	0.0	0.0	12.666300380675798	0.0
25	8.919301725706498E-4	0.0	0.0	12.990249419353457	0.0
26	8.919301725706498E-4	0.0	0.0	13.312771369755005	0.0
27	8.919301725706498E-4	0.0	0.0	13.684706251716966	0.0
28	8.919301725706498E-4	0.0	0.0	14.023818103328326	0.0
29	8.919301725706498E-4	0.0	0.0	14.394325897014175	0.0
30	8.919301725706498E-4	0.0	0.0	14.859913447096053	0.0
31	8.919301725706498E-4	0.0	0.0	15.215436813882715	0.0
32	8.919301725706498E-4	0.0	0.0	15.574884673428686	0.0
33	8.919301725706498E-4	0.0	0.0	15.953063066598641	0.0
34	8.919301725706498E-4	0.0	0.0	16.34818813304744	0.0
35	8.919301725706498E-4	0.0	0.0	16.75241088725646	0.0
36	8.919301725706498E-4	0.0	0.0	17.128091875943216	0.0
37	8.919301725706498E-4	0.0	0.0	17.498599669629066	0.0
38	0.0010703162070847796	0.0	0.0	17.91530944625407	0.0
39	0.0012487022415989097	0.0	0.0	18.505945606530357	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCAT	30	2.1635042E-6	45.000004	9
AACCGAT	20	7.030192E-4	45.0	16
ATTAGCG	20	7.030192E-4	45.0	1
CGTTTTT	1765	0.0	41.81303	1
CGTAAGG	60	3.6379788E-12	41.250004	2
ACGTAAG	40	3.453988E-7	39.375	1
TGCGACG	35	6.2425624E-6	38.57143	1
ATAGCGG	130	0.0	38.076927	2
CCCTAGC	210	0.0	37.500004	21
TCGACAC	30	1.1391759E-4	37.500004	37
AATCGAG	30	1.1391759E-4	37.500004	1
TAGTACG	30	1.1391759E-4	37.500004	1
TAGGGCG	145	0.0	37.24138	5
TAGGGAC	505	0.0	36.980198	5
CGAGGGT	80	0.0	36.5625	4
TACAGCG	75	1.8189894E-12	36.000004	1
GTACGAG	75	1.8189894E-12	36.000004	1
CTCACGA	25	0.00210619	36.0	24
AAATCGG	25	0.00210619	36.0	2
ACACGGT	25	0.00210619	36.0	32
>>END_MODULE