##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935413.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475004
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.713417150171367	31.0	31.0	34.0	30.0	34.0
2	31.733158036563903	31.0	31.0	34.0	30.0	34.0
3	31.79186912110214	33.0	31.0	34.0	30.0	34.0
4	35.53634074660424	37.0	35.0	37.0	33.0	37.0
5	35.57539304932169	37.0	35.0	37.0	33.0	37.0
6	35.54096386556745	37.0	35.0	37.0	33.0	37.0
7	35.77362295896456	37.0	35.0	37.0	35.0	37.0
8	35.653691337336106	37.0	35.0	37.0	33.0	37.0
9	37.421638554622696	39.0	37.0	39.0	34.0	39.0
10	36.74632845197093	39.0	37.0	39.0	32.0	39.0
11	36.757282043940684	39.0	35.0	39.0	32.0	39.0
12	36.82282464989768	39.0	35.0	39.0	33.0	39.0
13	36.84507077835134	39.0	37.0	39.0	33.0	39.0
14	37.92036698638327	40.0	37.0	41.0	33.0	41.0
15	37.9951705669847	40.0	37.0	41.0	33.0	41.0
16	37.93204057228992	40.0	37.0	41.0	33.0	41.0
17	37.87185792119645	40.0	37.0	41.0	33.0	41.0
18	37.729109649602954	39.0	36.0	41.0	33.0	41.0
19	37.609818022585074	39.0	37.0	41.0	33.0	41.0
20	37.557715303450074	39.0	35.0	41.0	33.0	41.0
21	37.526875563153155	39.0	35.0	41.0	33.0	41.0
22	37.617055856371735	39.0	35.0	41.0	33.0	41.0
23	37.64004303121658	39.0	35.0	41.0	33.0	41.0
24	37.63368308477402	39.0	35.0	41.0	33.0	41.0
25	37.32022046130138	39.0	35.0	41.0	32.0	41.0
26	37.469031839731876	39.0	35.0	41.0	33.0	41.0
27	37.455834056134265	39.0	35.0	41.0	33.0	41.0
28	37.13113152731346	39.0	35.0	41.0	32.0	41.0
29	36.90132925196419	39.0	35.0	41.0	31.0	41.0
30	36.78611759058871	39.0	35.0	41.0	31.0	41.0
31	36.785117599009695	39.0	35.0	41.0	31.0	41.0
32	36.68818788894409	39.0	35.0	41.0	31.0	41.0
33	36.438482623304225	39.0	35.0	41.0	30.0	41.0
34	36.31504366279021	39.0	35.0	41.0	30.0	41.0
35	36.22742545325934	39.0	35.0	41.0	29.0	41.0
36	36.11731269631414	39.0	35.0	41.0	29.0	41.0
37	36.05248166331231	39.0	35.0	41.0	28.0	41.0
38	35.88303677442716	39.0	35.0	41.0	27.0	41.0
39	35.75842729745434	39.0	35.0	41.0	26.0	41.0
40	35.68569738360098	39.0	35.0	41.0	26.0	41.0
41	35.501269462993996	39.0	35.0	41.0	25.0	41.0
42	35.56096369714781	39.0	35.0	41.0	26.0	41.0
43	35.415604921221714	39.0	35.0	41.0	24.0	41.0
44	35.31827310927908	39.0	35.0	40.0	24.0	41.0
45	35.30641636702007	39.0	35.0	40.0	25.0	41.0
46	35.12091898173489	38.0	34.0	40.0	24.0	41.0
47	35.1151274515583	38.0	34.0	40.0	24.0	41.0
48	34.99190743656896	38.0	34.0	40.0	24.0	41.0
49	34.729520172461704	38.0	34.0	40.0	23.0	41.0
50	34.49058113194836	38.0	34.0	40.0	22.0	41.0
51	33.245804245858984	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	1.0
12	4.0
13	5.0
14	14.0
15	11.0
16	33.0
17	74.0
18	173.0
19	335.0
20	697.0
21	1134.0
22	1735.0
23	2457.0
24	3559.0
25	5333.0
26	7377.0
27	8244.0
28	8389.0
29	8524.0
30	9344.0
31	11217.0
32	13684.0
33	17974.0
34	28557.0
35	34365.0
36	37991.0
37	55220.0
38	96445.0
39	122051.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.707025625047365	20.324881474682318	24.4204259332553	14.547666967015013
2	33.69697939385773	26.88861567481537	23.82064151038728	15.593763420939613
3	28.728600180209007	26.203568812052108	30.0069052050088	15.060925802730083
4	25.52673240646395	24.332637198844644	32.85467069750992	17.285959697181497
5	25.702941448914114	28.19618361108538	28.654916590176082	17.445958349824423
6	23.695168882788355	34.58476139148302	27.568188899461898	14.151880826266726
7	75.95009726233884	5.439743665316503	14.471036033380772	4.1391230389638825
8	77.53871546344872	6.85236334851917	11.50306102685451	4.10586016117759
9	71.02424400636626	8.174036429166913	13.157152360822225	7.6445672036446
10	34.98665274397689	32.568988892725116	19.644045102778083	12.80031326051991
11	26.206516155653425	27.201244621097924	29.751538934408973	16.84070028883967
12	23.472433916345967	22.092024488214836	34.83696979393858	19.59857180150062
13	24.392426169042785	23.83769399836633	34.577393032479726	17.192486800111155
14	19.148049279585013	30.273850325470942	31.36794637518842	19.210154019755624
15	17.477537031267104	26.46546134348342	37.650630310481596	18.406371314767874
16	19.79920169093313	28.17260486227484	31.638680937423686	20.389512509368345
17	20.85982433832136	25.520206145632457	30.390059873180014	23.229909642866165
18	24.243374792633325	24.88757989406405	30.084378236814846	20.784667076487775
19	23.322329917221747	27.08229825433049	28.787968101321255	20.80740372712651
20	23.638958829820382	25.834519288258623	29.862064319458366	20.664457562462633
21	23.852009667287014	27.74966105548585	30.15743025321892	18.24089902400822
22	20.997507389411457	26.356620154777644	30.27321875184209	22.37265370396881
23	19.796464871874765	27.281875521048242	30.124167375432627	22.797492231644366
24	20.272250338944513	25.659783917609115	30.579110912750206	23.488854830696162
25	19.77183350034947	29.373857904354487	28.641021970341303	22.213286624954737
26	19.72004446278347	26.82924775370313	29.101649670318565	24.349058113194836
27	21.726553881651522	26.089043460686646	29.096176032201832	23.088226625459995
28	21.564871032664986	26.28188394202996	31.113001153674496	21.040243871630555
29	21.82781618681106	25.2507347306549	31.234473814957347	21.686975267576695
30	23.349277058719505	25.87157160781804	29.722697072024655	21.0564542614378
31	23.337698208857187	27.664188091047652	27.406716575018315	21.591397125076842
32	23.152015561974217	28.48312856312789	29.42080487743261	18.944050997465283
33	21.71392240907445	27.145876666301756	29.81469629729434	21.325504627329455
34	23.623801062727892	26.314936295273306	30.543953314077356	19.51730932792145
35	20.714772928227973	26.263147257707303	31.135316755227326	21.8867630588374
36	23.509696760448335	27.208191931015318	29.516804069018367	19.76530723951798
37	21.388240941128917	27.693661527060826	30.06227315980497	20.855824372005287
38	21.646765079873013	27.53176815353134	28.549654318700473	22.271812447895176
39	24.245480038062837	25.274524004008388	29.075965676078518	21.404030281850257
40	23.648221909710234	25.35831277210297	30.84816127864186	20.145304039544932
41	21.48445065725762	25.68294161733375	29.77911764953558	23.053490075873047
42	23.210330860371702	25.891571439398405	29.970273934535292	20.927823765694605
43	23.295172251181043	26.213252941027864	28.069237311685796	22.422337496105296
44	22.225286523902955	25.73999376847353	27.888396729290697	24.146322978332815
45	20.76908826030939	25.23115594816044	28.003764178827968	25.995991612702205
46	22.224444425731154	27.655135535700754	26.99956210895066	23.120857929617436
47	20.541090180293217	25.056420577510924	30.718899209269818	23.683590032926038
48	21.234136975688624	24.900632415727024	29.505435743699	24.359794864885348
49	22.143813525780836	23.803799546951186	31.457840355028587	22.59454657223939
50	20.595826561460534	24.308847925491154	31.850258103089658	23.245067409958654
51	21.69118575843572	23.854114912716526	28.975966518176687	25.47873281067107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	391.5
2	539.0
3	3840.0
4	7141.0
5	4800.5
6	2460.0
7	2445.5
8	2431.0
9	2497.0
10	2563.0
11	2533.5
12	2504.0
13	2532.5
14	2561.0
15	2454.5
16	2348.0
17	2294.0
18	2240.0
19	2106.0
20	1972.0
21	1978.0
22	1984.0
23	2067.5
24	2151.0
25	2603.5
26	3526.0
27	3996.0
28	4643.5
29	5291.0
30	6638.5
31	7986.0
32	8856.5
33	9727.0
34	11109.0
35	12491.0
36	13770.5
37	15050.0
38	16154.0
39	17258.0
40	19783.0
41	22308.0
42	24665.5
43	27023.0
44	30379.0
45	33735.0
46	44068.0
47	54401.0
48	50277.0
49	46153.0
50	44288.0
51	42423.0
52	37875.5
53	33328.0
54	29185.5
55	25043.0
56	23046.5
57	21050.0
58	20114.5
59	19179.0
60	17338.0
61	15497.0
62	13546.0
63	11595.0
64	9133.0
65	6671.0
66	5292.5
67	3914.0
68	3112.5
69	2311.0
70	1964.5
71	1618.0
72	1436.5
73	1255.0
74	996.0
75	555.0
76	373.0
77	291.5
78	210.0
79	152.5
80	95.0
81	79.5
82	64.0
83	40.0
84	16.0
85	9.5
86	3.0
87	5.0
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.965039067631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20560390172264	31.217655410064815
2	8.70662597086032	7.133345460870874
3	3.34921838310941	4.116025857303147
4	1.9606447264626448	3.2127155126914775
5	1.4393802189228728	2.9482133450675376
6	1.1054372557616399	2.7170568221454228
7	0.9270679627411756	2.658416271842894
8	0.7576942021492815	2.483117807389025
9	0.6453725301567022	2.3793939819940655
>10	4.815303983184933	33.38930101777587
>50	0.0634179787422849	1.747998839652883
>100	0.017530172985669994	1.4204074669725066
>500	0.0030935599386476454	0.782121762111404
>1k	0.0036091532617555863	3.794230444118083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4461	0.9391499861053803	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	4211	0.8865188503675758	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	2628	0.5532584988757989	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	2622	0.5519953516180917	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	1777	0.37410211282431305	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1238	0.260629384173607	TruSeq Adapter, Index 22 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1027	0.2162087056109001	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTG	768	0.16168284898653484	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	646	0.13599885474648635	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	636	0.13389360931697417	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	571	0.12020951402514504	TruSeq Adapter, Index 20 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	546	0.11494640045136462	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	536	0.11284115502185246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTC	495	0.10420964876085254	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	479	0.10084125607363306	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012631472577073035	0.0	0.0	0.13115679025860835	0.0
2	0.0012631472577073035	0.0	0.0	0.6732574883579928	0.0
3	0.0012631472577073035	0.0	0.0	1.065885760962013	0.0
4	0.0012631472577073035	0.0	0.0	1.6825121472661282	0.0
5	0.0012631472577073035	0.0	0.0	3.4744970568668894	0.0
6	0.0012631472577073035	0.0	0.0	4.704381436787901	0.0
7	0.0012631472577073035	0.0	0.0	5.50500627363138	0.0
8	0.0012631472577073035	0.0	0.0	6.598470749720002	0.0
9	0.0012631472577073035	0.0	0.0	7.036151274515583	0.0
10	0.0012631472577073035	0.0	0.0	8.376771564028935	0.0
11	0.0012631472577073035	0.0	0.0	9.366236915899655	0.0
12	0.0012631472577073035	0.0	0.0	10.79717223433908	0.0
13	0.0012631472577073035	0.0	0.0	11.410009178870073	0.0
14	0.0012631472577073035	0.0	0.0	11.69505941002602	0.0
15	0.0012631472577073035	0.0	0.0	12.043267004067333	0.0
16	0.0012631472577073035	0.0	0.0	12.532526041885964	0.0
17	0.0012631472577073035	0.0	0.0	13.1702048824852	0.0
18	0.0012631472577073035	0.0	0.0	13.915461764532509	0.0
19	0.0012631472577073035	0.0	0.0	14.415878603127553	0.0
20	0.0012631472577073035	0.0	0.0	14.863874830527743	0.0
21	0.0012631472577073035	0.0	0.0	15.353765441975225	0.0
22	0.0012631472577073035	0.0	0.0	15.92681324788844	0.0
23	0.0012631472577073035	0.0	0.0	16.48070332039309	0.0
24	0.0014736718006585208	0.0	0.0	16.926173253277867	0.0
25	0.0014736718006585208	0.0	0.0	17.320064673139594	0.0
26	0.0014736718006585208	0.0	0.0	17.728692811007907	0.0
27	0.0014736718006585208	0.0	0.0	18.14616297968017	0.0
28	0.0014736718006585208	0.0	0.0	18.526791353335973	0.0
29	0.0014736718006585208	0.0	0.0	18.947629914695455	0.0
30	0.0014736718006585208	0.0	0.0	19.44994147417706	0.0
31	0.0014736718006585208	0.0	0.0	19.936463692937323	0.0
32	0.0014736718006585208	0.0	0.0	20.383617822165707	0.0
33	0.0014736718006585208	0.0	0.0	20.90403449234112	0.0
34	0.0014736718006585208	0.0	0.0	21.464661350220208	0.0
35	0.0014736718006585208	0.0	0.0	21.95434143712474	0.0
36	0.0014736718006585208	0.0	0.0	22.389495667404905	0.0
37	0.0014736718006585208	0.0	0.0	22.83475507574673	0.0
38	0.001684196343609738	0.0	0.0	23.28611969583414	0.0
39	0.001684196343609738	0.0	0.0	23.801062727892816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	30	2.162802E-6	45.000004	2
TCACGAC	35	1.209919E-7	45.0	25
CGACGGT	40	6.8012014E-9	45.0	28
ATTAACG	20	7.0291024E-4	45.0	1
TCGATAG	20	7.0291024E-4	45.0	1
TCTACGG	20	7.0291024E-4	45.0	2
TGCGAAG	20	7.0291024E-4	45.0	1
GGTGCGT	20	7.0291024E-4	45.0	1
TATAGCG	20	7.0291024E-4	45.0	1
CGGTCTA	40	6.8012014E-9	45.0	31
TGTACCG	25	3.8873375E-5	45.0	1
GCCACGG	35	1.209919E-7	45.0	2
CGTTTTT	2635	0.0	43.37761	1
ACGGGTA	65	0.0	41.53846	5
CTAACGG	65	0.0	41.53846	2
ATTGCGG	65	0.0	41.53846	2
GCGATAT	60	3.6379788E-12	41.250004	9
CGAATAT	115	0.0	41.086956	14
ACGGTCT	50	1.0786607E-9	40.5	30
CGTGCGG	95	0.0	40.263157	2
>>END_MODULE