FastQCFastQC Report
Sat 14 Jan 2017
SRR2935412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599161
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG83411.3921133051049717No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC81391.358399495294253No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC80921.3505551930115611No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.6886296003912137No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC30470.5085444479864343No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT20830.3476528011669651No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT15760.2630344765430327No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC14890.24851417231762413No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT14270.23816636930641347No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG13610.2271509661009311No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT12850.21446656240976966Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA12670.21146236153554723No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT12470.2081243605641889No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC12150.20278355901001566No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT8580.14320024167127032No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT6770.11299133288047787No Hit
CGTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT6660.11115543234623082No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC6450.10765053132630462No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC6340.10581463079205755No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC6180.10314423001497094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG253.8885617E-545.0000041
CACCGCT253.8885617E-545.00000416
ATCGCTA253.8885617E-545.00000412
TCACGAC406.8048394E-945.025
TCGTTGA207.03058E-445.024
CGCCTGG207.03058E-445.012
CCACGCT207.03058E-445.038
GCATCGA207.03058E-445.09
TCTACGG302.1637552E-644.9999962
CGTTTTT29100.043.453611
TAGGGCG2300.041.086965
GCTACGA1050.040.71428310
GCGATAT501.0804797E-940.5000049
TGGGCGA2650.039.9056636
TACGGCT9200.039.6195687
TACGAAT1100.038.86363612
CGAATAT1100.038.86363614
GTGTACG356.2432828E-638.5714261
ACGGCTG9700.037.577328
CCGGGAT900.037.55
Produced by FastQC (version 0.11.5)