##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599161
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69759547100028	31.0	31.0	34.0	30.0	34.0
2	31.760898990421605	33.0	31.0	34.0	30.0	34.0
3	31.849616380238366	33.0	31.0	34.0	30.0	34.0
4	35.60925026829183	37.0	35.0	37.0	33.0	37.0
5	35.62486710583633	37.0	35.0	37.0	33.0	37.0
6	35.5630439898458	37.0	35.0	37.0	33.0	37.0
7	35.855773323030036	37.0	35.0	37.0	35.0	37.0
8	35.642106211852905	37.0	35.0	37.0	33.0	37.0
9	37.44732551017172	39.0	37.0	39.0	34.0	39.0
10	36.82455133094444	39.0	37.0	39.0	32.0	39.0
11	36.79486648830615	39.0	35.0	39.0	32.0	39.0
12	36.87682275715542	39.0	35.0	39.0	33.0	39.0
13	36.838048537872126	39.0	37.0	39.0	33.0	39.0
14	37.896815713973375	40.0	37.0	41.0	33.0	41.0
15	37.92410887891568	40.0	37.0	41.0	33.0	41.0
16	37.91409821400258	40.0	37.0	41.0	33.0	41.0
17	37.868010434591035	40.0	37.0	41.0	33.0	41.0
18	37.76170177965522	39.0	37.0	41.0	33.0	41.0
19	37.61704783856092	39.0	36.0	41.0	33.0	41.0
20	37.57940353260643	39.0	36.0	41.0	33.0	41.0
21	37.63132613771591	39.0	36.0	41.0	33.0	41.0
22	37.75083491749296	39.0	36.0	41.0	33.0	41.0
23	37.72290252536464	39.0	36.0	41.0	33.0	41.0
24	37.72714178659826	39.0	36.0	41.0	33.0	41.0
25	37.50702065054301	39.0	35.0	41.0	33.0	41.0
26	37.51804940575238	39.0	35.0	41.0	33.0	41.0
27	37.54494868658007	39.0	36.0	41.0	33.0	41.0
28	37.42548330081564	39.0	36.0	41.0	33.0	41.0
29	37.38088093183635	39.0	35.0	41.0	33.0	41.0
30	37.28083770472377	39.0	35.0	41.0	32.0	41.0
31	37.233731835015966	39.0	35.0	41.0	32.0	41.0
32	37.12030322400824	39.0	35.0	41.0	32.0	41.0
33	36.91462394915557	39.0	35.0	41.0	31.0	41.0
34	36.755272789784385	39.0	35.0	41.0	31.0	41.0
35	36.69695457481378	39.0	35.0	41.0	31.0	41.0
36	36.45635647179974	39.0	35.0	41.0	30.0	41.0
37	36.47652968067014	39.0	35.0	41.0	30.0	41.0
38	36.39618399728954	39.0	35.0	41.0	30.0	41.0
39	36.36238673745454	39.0	35.0	41.0	30.0	41.0
40	36.32694050513969	39.0	35.0	41.0	30.0	41.0
41	36.09301506606739	39.0	35.0	41.0	29.0	41.0
42	36.16877266711285	39.0	35.0	41.0	30.0	41.0
43	36.04505466811091	39.0	35.0	41.0	29.0	41.0
44	35.97314912018639	39.0	35.0	40.0	29.0	41.0
45	36.032313518403235	39.0	35.0	40.0	29.0	41.0
46	35.92267020049703	39.0	35.0	40.0	29.0	41.0
47	35.836663267469014	39.0	35.0	40.0	28.0	41.0
48	35.75105522555707	39.0	35.0	40.0	28.0	41.0
49	35.7075460518959	39.0	35.0	40.0	28.0	41.0
50	35.56415387516878	38.0	35.0	40.0	27.0	41.0
51	34.3480566992845	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	1.0
11	6.0
12	11.0
13	10.0
14	14.0
15	14.0
16	34.0
17	85.0
18	160.0
19	339.0
20	670.0
21	1213.0
22	1769.0
23	2362.0
24	3310.0
25	5012.0
26	6974.0
27	7991.0
28	8103.0
29	8802.0
30	9852.0
31	12744.0
32	16642.0
33	22694.0
34	36915.0
35	44790.0
36	48645.0
37	71574.0
38	129151.0
39	159229.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.432232738779724	19.90449979220944	24.158615130157003	12.50465233885383
2	31.90661608482528	29.359053743484637	23.586481763666193	15.147848408023886
3	28.469309584569093	28.167888096855435	28.618518227988805	14.744284090586671
4	24.94070875774625	23.672101488581536	34.79064892407884	16.59654082959338
5	27.47408459495862	26.72403577669441	27.575893624585046	18.225986003761925
6	23.52589704603604	35.663703078137594	26.86723601836568	13.943163857460682
7	73.86679039523601	4.940408337658826	16.616401935372963	4.576399331732206
8	73.98846053064202	8.777440454235172	12.132799030644518	5.101299984478295
9	68.63914707399181	6.992778234898466	13.228164049395739	11.139910641713998
10	36.81097401199344	28.718491357080982	20.366145326548292	14.104389304377287
11	27.47141419418153	23.979197577946497	29.97424732250597	18.575140905366002
12	24.166292532391125	20.794577751222125	33.85934665306988	21.17978306331687
13	23.195101149774437	23.51454784273342	36.27038475468196	17.01996625281018
14	20.01665662484708	29.14158298020065	30.94193380410274	19.899826590849536
15	17.626981729451682	25.38149178601411	37.961416046772065	19.030110437762136
16	19.702216933345127	26.542448523852517	31.490367363696905	22.26496717910545
17	21.100672440295682	26.076630488299475	31.154731366026827	21.66796570537802
18	21.35436051411891	25.511506923848515	31.72335983149771	21.410772730534863
19	20.844814665841067	28.227471414194184	29.17546369005993	21.752250229904817
20	22.13678794180529	27.36576646343804	32.39329662644932	18.10414896830735
21	21.359033715478812	29.380583849749904	31.092477647910993	18.16790478686029
22	20.43090254539264	24.12606962068626	31.901275283271108	23.541752550649992
23	19.66032502115458	28.52939360205354	30.97297721313637	20.837304163655514
24	21.133885549960695	24.934700355997805	30.673892326102663	23.257521767938837
25	19.332366425718632	30.40768674863684	28.85685149734379	21.403095328300743
26	18.98421292440596	27.515809607100593	30.43105275543635	23.06892471305709
27	22.07753842456368	27.387797269849006	28.40939246713321	22.125271838454104
28	18.240673208035904	28.304078536486855	32.03596362246542	21.419284633011827
29	21.376391320529876	26.737387780579848	30.033663739796147	21.852557159094133
30	23.64306088013072	27.859123007004793	29.434826365534473	19.06298974733002
31	23.987375680326323	28.131336986219065	26.77510719155619	21.10618014189842
32	24.536977540260462	28.276039328327446	28.511702196905343	18.675280934506752
33	24.439174111799666	25.630506658477437	27.93022242769473	22.000096802028168
34	24.739427299173343	27.314361248479123	28.177568299672373	19.768643152675157
35	25.371310883051468	25.26382725177373	28.422410670921504	20.942451194253298
36	23.451292724326184	29.655468229741256	28.41590156902736	18.477337476905205
37	23.955998471195556	26.356021169602162	31.53376137632456	18.154218982877723
38	23.031205302080743	27.917204223906428	27.64248674396364	21.409103730049186
39	24.90048584604138	24.62376556551578	30.514836579817445	19.960912008625396
40	27.382122668197695	23.414074013495537	29.34603553969634	19.857767778610423
41	23.453295524909	25.848144321809997	28.752038266843133	21.94652188643787
42	23.605675269251503	24.969749366197068	30.586102900555943	20.838472463995487
43	22.84661384836463	24.998623074599315	29.715886047322837	22.43887702971322
44	22.96978608420775	24.922516652452344	29.106367069952814	23.001330193387087
45	21.827355251760377	23.63454897765375	28.71298365547824	25.825112115107622
46	25.244967546285558	26.151401710057897	27.648328245663517	20.955302497993028
47	21.301786998820017	24.527964937637798	32.80670804675204	21.363540016790143
48	22.411004721602374	24.66999687896909	29.790490369032696	23.128508030395835
49	21.801151944135217	22.4191828239822	33.15703124869609	22.62263398318649
50	21.83286295336312	22.69857350528489	31.658602612653358	23.809960928698633
51	21.758258631653263	22.62647268430355	28.459963181849286	27.1553055021939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	361.5
2	529.0
3	3731.5
4	6934.0
5	4767.5
6	2601.0
7	2531.5
8	2462.0
9	2535.5
10	2609.0
11	2645.5
12	2682.0
13	2555.0
14	2428.0
15	2393.0
16	2358.0
17	2308.5
18	2259.0
19	2205.0
20	2151.0
21	2185.5
22	2220.0
23	2416.5
24	2613.0
25	3023.5
26	3928.0
27	4422.0
28	5266.5
29	6111.0
30	7605.5
31	9100.0
32	10598.5
33	12097.0
34	14154.5
35	16212.0
36	17344.5
37	18477.0
38	20217.5
39	21958.0
40	24685.0
41	27412.0
42	30991.5
43	34571.0
44	38946.0
45	43321.0
46	65501.0
47	87681.0
48	77461.0
49	67241.0
50	63224.5
51	59208.0
52	50228.0
53	41248.0
54	35454.0
55	29660.0
56	26833.0
57	24006.0
58	21306.0
59	18606.0
60	16769.0
61	14932.0
62	12693.0
63	10454.0
64	8681.5
65	6909.0
66	5735.5
67	4562.0
68	3454.0
69	2346.0
70	1955.5
71	1565.0
72	1076.5
73	588.0
74	536.5
75	377.5
76	270.0
77	185.5
78	101.0
79	90.0
80	79.0
81	58.5
82	38.0
83	25.0
84	12.0
85	10.5
86	9.0
87	4.5
88	0.0
89	1.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	599161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.328211333932835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42991631152651	29.66523689600582
2	9.432961697831479	7.419630223144205
3	3.57998002469931	4.223826329478976
4	2.0791506325902556	3.2707710189435883
5	1.4276307240303578	2.8073081410740732
6	1.031551234825688	2.434143897900242
7	0.8782075235157019	2.417683175591272
8	0.7367238756510202	2.31792258210859
9	0.617005320243969	2.1839144065844147
>10	4.689406471507632	31.66782089598271
>50	0.061714395723298426	1.6408983422485572
>100	0.026813840891105688	1.6989817575530988
>500	0.002979315654567299	0.7704840423661984
>1k	0.004681781742891469	3.368335951452316
>5k	0.0012768495662431282	4.113042339565984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	8341	1.3921133051049717	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	8139	1.358399495294253	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	8092	1.3505551930115611	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4126	0.6886296003912137	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3047	0.5085444479864343	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	2083	0.3476528011669651	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	1576	0.2630344765430327	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1489	0.24851417231762413	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	1427	0.23816636930641347	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	1361	0.2271509661009311	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1285	0.21446656240976966	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	1267	0.21146236153554723	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	1247	0.2081243605641889	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	1215	0.20278355901001566	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	858	0.14320024167127032	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	677	0.11299133288047787	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	666	0.11115543234623082	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	645	0.10765053132630462	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	634	0.10581463079205755	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	618	0.10314423001497094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.345002428395707E-4	0.0	0.0	0.23399386809221562	0.0
2	8.345002428395707E-4	0.0	0.0	1.248078563190862	0.0
3	8.345002428395707E-4	0.0	0.0	1.799850123756386	0.0
4	8.345002428395707E-4	0.0	0.0	2.7032800866545053	0.0
5	8.345002428395707E-4	0.0	0.0	5.352317657524439	0.0
6	8.345002428395707E-4	0.0	0.0	6.6808420441250345	0.0
7	8.345002428395707E-4	0.0	0.0	7.836124180312137	0.0
8	8.345002428395707E-4	0.0	0.0	9.303175607224102	0.0
9	8.345002428395707E-4	0.0	0.0	9.87948147492911	0.0
10	0.001001400291407485	0.0	0.0	12.41102141160723	0.0
11	0.001001400291407485	0.0	0.0	13.908281747309989	0.0
12	0.001001400291407485	0.0	0.0	16.47670659472162	0.0
13	0.001001400291407485	0.0	0.0	17.07671226932327	0.0
14	0.001001400291407485	0.0	0.0	17.36645075363717	0.0
15	0.001001400291407485	0.0	0.0	17.937248919739435	0.0
16	0.001001400291407485	0.0	0.0	18.63155312178196	0.0
17	0.001001400291407485	0.0	0.0	19.54599848788556	0.0
18	0.001001400291407485	0.0	0.0	20.514352569676596	0.0
19	0.001001400291407485	0.0	0.0	21.345848611641944	0.0
20	0.001001400291407485	0.0	0.0	21.956535889351944	0.0
21	0.001001400291407485	0.0	0.0	22.62580508410928	0.0
22	0.001168300339975399	0.0	0.0	23.4179127146126	0.0
23	0.001168300339975399	0.0	0.0	24.18782263865639	0.0
24	0.001168300339975399	0.0	0.0	24.766798907138483	0.0
25	0.001168300339975399	0.0	0.0	25.29336856037025	0.0
26	0.001168300339975399	0.0	0.0	25.819771313553453	0.0
27	0.001168300339975399	0.0	0.0	26.37221047431325	0.0
28	0.001168300339975399	0.0	0.0	26.910296230896204	0.0
29	0.001168300339975399	0.0	0.0	27.4538896890819	0.0
30	0.001168300339975399	0.0	0.0	28.119320182722173	0.0
31	0.001168300339975399	0.0	0.0	28.694123949990068	0.0
32	0.001168300339975399	0.0	0.0	29.263586915703794	0.0
33	0.001168300339975399	0.0	0.0	29.793160769809784	0.0
34	0.001168300339975399	0.0	0.0	30.33124652639274	0.0
35	0.0013352003885433131	0.0	0.0	30.881015286375447	0.0
36	0.0013352003885433131	0.0	0.0	31.40758493960722	0.0
37	0.0013352003885433131	0.0	0.0	31.94099749483027	0.0
38	0.0013352003885433131	0.0	0.0	32.48459095301597	0.0
39	0.0013352003885433131	0.0	0.0	33.05989542042957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	25	3.8885617E-5	45.000004	1
CACCGCT	25	3.8885617E-5	45.000004	16
ATCGCTA	25	3.8885617E-5	45.000004	12
TCACGAC	40	6.8048394E-9	45.0	25
TCGTTGA	20	7.03058E-4	45.0	24
CGCCTGG	20	7.03058E-4	45.0	12
CCACGCT	20	7.03058E-4	45.0	38
GCATCGA	20	7.03058E-4	45.0	9
TCTACGG	30	2.1637552E-6	44.999996	2
CGTTTTT	2910	0.0	43.45361	1
TAGGGCG	230	0.0	41.08696	5
GCTACGA	105	0.0	40.714283	10
GCGATAT	50	1.0804797E-9	40.500004	9
TGGGCGA	265	0.0	39.905663	6
TACGGCT	920	0.0	39.619568	7
TACGAAT	110	0.0	38.863636	12
CGAATAT	110	0.0	38.863636	14
GTGTACG	35	6.2432828E-6	38.571426	1
ACGGCTG	970	0.0	37.57732	8
CCGGGAT	90	0.0	37.5	5
>>END_MODULE