##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935408.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348519
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65173778187129	31.0	31.0	34.0	30.0	34.0
2	31.704678941463737	31.0	31.0	34.0	30.0	34.0
3	31.787274151480982	33.0	31.0	34.0	30.0	34.0
4	35.54315259713244	37.0	35.0	37.0	33.0	37.0
5	35.57726838422008	37.0	35.0	37.0	33.0	37.0
6	35.53921880873066	37.0	35.0	37.0	33.0	37.0
7	35.821725644799855	37.0	35.0	37.0	35.0	37.0
8	35.67474657048827	37.0	35.0	37.0	35.0	37.0
9	37.46225026469145	39.0	37.0	39.0	35.0	39.0
10	36.546633038657866	39.0	35.0	39.0	32.0	39.0
11	36.668807152551224	39.0	35.0	39.0	32.0	39.0
12	36.81484510170177	39.0	35.0	39.0	33.0	39.0
13	36.81602150815307	39.0	37.0	39.0	33.0	39.0
14	37.80808506853285	39.0	37.0	41.0	33.0	41.0
15	37.93462910200018	40.0	37.0	41.0	33.0	41.0
16	37.87903385468224	39.0	37.0	41.0	33.0	41.0
17	37.86578923961104	39.0	37.0	41.0	33.0	41.0
18	37.78600018937274	39.0	37.0	41.0	33.0	41.0
19	37.64577540966203	39.0	36.0	41.0	33.0	41.0
20	37.66542713596676	39.0	36.0	41.0	33.0	41.0
21	37.62638191891977	39.0	36.0	41.0	33.0	41.0
22	37.74895773257699	39.0	36.0	41.0	33.0	41.0
23	37.76093986267607	39.0	36.0	41.0	33.0	41.0
24	37.76456089911884	39.0	36.0	41.0	33.0	41.0
25	37.46804047985906	39.0	36.0	41.0	33.0	41.0
26	37.5936692117216	39.0	36.0	41.0	33.0	41.0
27	37.59962871464683	39.0	36.0	41.0	33.0	41.0
28	37.40313727515573	39.0	36.0	41.0	33.0	41.0
29	37.32145449745925	39.0	36.0	41.0	32.0	41.0
30	37.18851196061047	39.0	36.0	41.0	32.0	41.0
31	37.224989168452794	39.0	36.0	41.0	32.0	41.0
32	37.202953067121165	39.0	36.0	41.0	32.0	41.0
33	37.01304376518927	39.0	36.0	41.0	31.0	41.0
34	36.930919117752545	40.0	36.0	41.0	31.0	41.0
35	36.87786892536705	40.0	36.0	41.0	31.0	41.0
36	36.76169448437531	39.0	35.0	41.0	31.0	41.0
37	36.67403785733346	39.0	35.0	41.0	30.0	41.0
38	36.56594332016332	39.0	35.0	41.0	30.0	41.0
39	36.480794447361546	39.0	35.0	41.0	30.0	41.0
40	36.413004742926496	39.0	35.0	41.0	30.0	41.0
41	36.25955543313277	39.0	35.0	41.0	29.0	41.0
42	36.307650372002676	39.0	35.0	41.0	30.0	41.0
43	36.21892350201854	39.0	35.0	41.0	29.0	41.0
44	36.12364892588352	39.0	35.0	41.0	29.0	41.0
45	36.13069301817118	39.0	35.0	41.0	29.0	41.0
46	35.960974867941204	39.0	35.0	40.0	28.0	41.0
47	35.908145610425834	39.0	35.0	40.0	28.0	41.0
48	35.81108347034164	39.0	35.0	40.0	28.0	41.0
49	35.65953649585819	39.0	35.0	40.0	27.0	41.0
50	35.51748685150594	39.0	35.0	40.0	26.0	41.0
51	34.19499080394469	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	7.0
12	6.0
13	7.0
14	8.0
15	17.0
16	21.0
17	50.0
18	88.0
19	196.0
20	328.0
21	587.0
22	952.0
23	1294.0
24	1998.0
25	2892.0
26	4209.0
27	4674.0
28	4816.0
29	5133.0
30	5991.0
31	7419.0
32	9676.0
33	12768.0
34	20780.0
35	25225.0
36	27839.0
37	41442.0
38	76233.0
39	93830.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02703152482361	21.846441657413227	25.60979458795647	11.516732229806696
2	32.42606572381994	26.347200583038514	25.005523371753046	16.221210321388504
3	29.289364424895055	25.358158378739752	29.54214834772279	15.8103288486424
4	25.57679782163957	24.94584226397987	31.997394690102976	17.479965224277585
5	25.45370553685739	28.69054484834399	27.878824396948232	17.97692521785039
6	23.32067979077181	35.68585930752699	27.058209165067044	13.935251736634157
7	77.33150846869181	5.4166343872213565	12.809344684220946	4.44251245986589
8	78.55812739047225	6.184454792995504	10.3130675802467	4.94435023628554
9	72.67839056120326	7.296015425270933	12.167772775659289	7.857821237866515
10	35.60953635239399	27.340833641781366	21.73826964957434	15.311360356250306
11	26.58420344371469	27.39276768268014	27.16207724686459	18.86095162674058
12	22.908937532817436	22.073975880798464	34.389516783876914	20.627569802507182
13	23.73299590553169	24.68330277545844	35.12491428013968	16.45878703887019
14	19.87237424645428	29.91458141449964	31.32827765487678	18.8847666841693
15	18.313779162685535	26.781897113213336	37.0447522229778	17.859571501123327
16	20.92712305498409	26.957210367297048	31.07176366281322	21.043902914905644
17	21.35034244904869	26.266573701864175	29.895357211515012	22.487726637572127
18	23.600721911861335	25.046554133347104	30.985972070389277	20.366751884402284
19	22.6509888987401	28.252405177336097	28.56745256356181	20.529153360361992
20	21.969247013792646	27.580418858082346	30.60234879590496	19.84798533222005
21	21.87742992491084	28.751086741325437	30.915387683311383	18.45609565045234
22	20.021577015887225	26.04678654535334	30.29160533572058	23.640031103038858
23	18.69109001230923	27.844679917020304	30.178268616632092	23.28596145403837
24	20.33002504884956	26.303874394222408	30.003529219353897	23.362571337574135
25	19.378857393714547	29.516898648280293	28.21252212935306	22.891721828652095
26	18.855786915490977	27.980970908329244	29.639130147854203	23.524112028325572
27	20.507059873349803	27.081163437287493	30.517991845494798	21.89378484386791
28	19.248018042057964	25.84564973502162	32.83493869774675	22.07139352517366
29	19.76735845104571	24.728637463093833	31.699563008042603	23.804441077817852
30	20.49787816446162	25.785681698845686	32.6223247513048	21.094115385387884
31	20.559567771054088	26.90986718084236	30.07583517684832	22.454729871255225
32	22.088322300936248	27.297220524562505	29.440288764744533	21.174168409756714
33	20.57190569237258	25.484980732757755	31.464568646185718	22.478544928683945
34	20.311087774267687	25.15329149917221	30.480691153136558	24.054929573423543
35	20.801448414577113	25.977349871886467	29.538418278486965	23.68278343504945
36	20.55095991897142	26.6126093555875	31.314505091544508	21.521925633896576
37	20.00263974130535	27.594191421414614	31.210350081344203	21.19281875593583
38	20.9606936781065	26.972130644240345	30.072392036015255	21.994783641637902
39	22.717843216582168	25.396606784709014	29.03858900088661	22.84696099782221
40	22.6535712543649	24.24602388965881	30.39260413349057	22.707800722485718
41	19.829334986040934	25.864300081200735	28.518674735093352	25.787690197664975
42	21.64903491631733	26.275755410752353	29.856334948740244	22.218874724190073
43	20.99799437046474	26.347774439844024	30.027918133588127	22.62631305610311
44	20.57276647758085	25.083854825705345	29.792636843328484	24.550741853385325
45	20.38626301578967	24.244876176047793	29.015634728666157	26.353226079496384
46	22.212275370926694	25.397754498320037	28.74620895847859	23.643761172274683
47	19.23137619469814	24.94785076279916	32.11187912280249	23.708893919700216
48	20.666018208476437	24.700231551221023	30.2015098172553	24.432240423047237
49	21.239014228779492	22.91783231330286	32.403972236807746	23.439181221109898
50	20.460864400506143	22.846100212613944	31.942017508371134	24.751017878508776
51	20.37363816606842	22.879096978930846	28.86270188999739	27.88456296500334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	208.0
2	296.0
3	1631.5
4	2967.0
5	2086.0
6	1205.0
7	1235.0
8	1265.0
9	1255.5
10	1246.0
11	1293.0
12	1340.0
13	1356.0
14	1372.0
15	1290.0
16	1208.0
17	1197.5
18	1187.0
19	1195.0
20	1203.0
21	1164.0
22	1125.0
23	1306.0
24	1487.0
25	1727.5
26	2351.5
27	2735.0
28	3263.5
29	3792.0
30	4911.5
31	6031.0
32	6651.0
33	7271.0
34	8328.0
35	9385.0
36	10437.5
37	11490.0
38	12622.0
39	13754.0
40	15560.5
41	17367.0
42	19849.0
43	22331.0
44	25285.5
45	28240.0
46	35991.0
47	43742.0
48	42453.0
49	41164.0
50	39414.0
51	37664.0
52	31557.5
53	25451.0
54	21324.0
55	17197.0
56	15074.0
57	12951.0
58	11616.0
59	10281.0
60	8972.0
61	7663.0
62	6316.5
63	4970.0
64	3974.0
65	2978.0
66	2373.5
67	1769.0
68	1353.5
69	938.0
70	750.5
71	563.0
72	504.5
73	446.0
74	310.0
75	133.0
76	92.0
77	70.5
78	49.0
79	29.5
80	10.0
81	16.5
82	23.0
83	13.0
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	348519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.65606667653125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22534888595249	36.8025637562734
2	9.291108939512448	8.855554142406415
3	3.5840367257462877	5.124032795199055
4	2.1086451346783432	4.019589325414974
5	1.473164202792475	3.5102605736878596
6	1.0144826865833998	2.900775273240304
7	0.8090143776944057	2.6988110187973926
8	0.6365493138417394	2.4268349234673723
9	0.5552725853980409	2.3815896618041044
>10	3.2372081854366686	24.637248974020302
>50	0.04344597490915961	1.4202809811925141
>100	0.01568882427275208	1.5652134805893614
>500	0.0030170815909138613	0.9630534533334136
>1k	0.0030170815909138613	2.69419164057353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	2364	0.6782987441143812	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	2029	0.582177729191235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	1983	0.5689790226644745	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.48175278822675377	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1314	0.37702392122093775	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	856	0.24561071275884527	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	753	0.21605708727501227	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	717	0.20572766477580848	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	512	0.14690734221089813	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	511	0.14662041380814245	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	478	0.13715177651720567	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	405	0.11620600311604247	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	358	0.10272036818652641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.607852082669811E-4	0.0	0.0	0.23040350741279528	0.0
2	8.607852082669811E-4	0.0	0.0	1.1416881145647726	0.0
3	8.607852082669811E-4	0.0	0.0	1.65758538271945	0.0
4	8.607852082669811E-4	0.0	0.0	2.3760541032196234	0.0
5	8.607852082669811E-4	0.0	0.0	4.459154307225718	0.0
6	8.607852082669811E-4	0.0	0.0	5.746888978793121	0.0
7	8.607852082669811E-4	0.0	0.0	6.769788734617051	0.0
8	8.607852082669811E-4	0.0	0.0	8.23771444311501	0.0
9	8.607852082669811E-4	0.0	0.0	8.79837254209957	0.0
10	8.607852082669811E-4	0.0	0.0	10.583927992448045	0.0
11	8.607852082669811E-4	0.0	0.0	12.23720944912616	0.0
12	8.607852082669811E-4	0.0	0.0	14.138970902590676	0.0
13	8.607852082669811E-4	0.0	0.0	14.810670293441678	0.0
14	8.607852082669811E-4	0.0	0.0	15.118831398001257	0.0
15	8.607852082669811E-4	0.0	0.0	15.56557892109182	0.0
16	8.607852082669811E-4	0.0	0.0	16.38418565415372	0.0
17	8.607852082669811E-4	0.0	0.0	17.456437095251623	0.0
18	8.607852082669811E-4	0.0	0.0	18.692524654323005	0.0
19	8.607852082669811E-4	0.0	0.0	19.491046399192008	0.0
20	8.607852082669811E-4	0.0	0.0	20.175083711361506	0.0
21	8.607852082669811E-4	0.0	0.0	20.997420513659225	0.0
22	8.607852082669811E-4	0.0	0.0	21.908418192408448	0.0
23	8.607852082669811E-4	0.0	0.0	22.8438047853919	0.0
24	8.607852082669811E-4	0.0	0.0	23.531859095199973	0.0
25	8.607852082669811E-4	0.0	0.0	24.17630028778919	0.0
26	8.607852082669811E-4	0.0	0.0	24.82734083364178	0.0
27	0.0011477136110226414	0.0	0.0	25.474938238661306	0.0
28	0.001434642013778302	0.0	0.0	26.105606867918247	0.0
29	0.001434642013778302	0.0	0.0	26.79193960730979	0.0
30	0.001434642013778302	0.0	0.0	27.536805740863482	0.0
31	0.001434642013778302	0.0	0.0	28.24695353768374	0.0
32	0.001434642013778302	0.0	0.0	28.907175792424518	0.0
33	0.001434642013778302	0.0	0.0	29.614741233619974	0.0
34	0.001434642013778302	0.0	0.0	30.280702056415862	0.0
35	0.001434642013778302	0.0	0.0	30.921413179769253	0.0
36	0.001434642013778302	0.0	0.0	31.54175238652699	0.0
37	0.001434642013778302	0.0	0.0	32.180168082658334	0.0
38	0.001434642013778302	0.0	0.0	32.80595892906843	0.0
39	0.001434642013778302	0.0	0.0	33.50864658741704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	55	1.8189894E-12	45.000004	2
CAATTGG	20	7.026514E-4	45.000004	2
CCGCTCA	20	7.026514E-4	45.000004	28
GCCGCAT	20	7.026514E-4	45.000004	19
TCAGCGA	20	7.026514E-4	45.000004	18
CGGGCTA	20	7.026514E-4	45.000004	6
CGCCAGT	20	7.026514E-4	45.000004	26
TATTGCG	50	2.1827873E-11	45.0	1
TCCGGGA	35	1.2086639E-7	45.0	4
TCGTTAG	30	2.161134E-6	44.999996	1
TAGCGTG	30	2.161134E-6	44.999996	36
CCGTACG	30	2.161134E-6	44.999996	1
TAGTGCG	30	2.161134E-6	44.999996	1
ACGACAG	30	2.161134E-6	44.999996	1
CGTTTTT	1190	0.0	42.73109	1
CGTTAGG	80	0.0	42.187504	2
ACAAGCG	75	0.0	42.0	14
TACACGC	70	0.0	41.785713	35
ACCCGCA	50	1.0768417E-9	40.5	32
CCGCACT	50	1.0768417E-9	40.5	34
>>END_MODULE