FastQCFastQC Report
Sat 14 Jan 2017
SRR2935407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769390
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144361.8762916076372191No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC79161.028867024525923No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTT34980.4546458883011217No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG34420.44736739494924554No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC32240.4190332601151561No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC25940.3371502099065493No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT23100.3002378507648917No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTT22060.2867206488256931No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT19660.25552710588908095No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT18260.23733087250939056No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG18190.23642106084040604No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC17900.23265184106889872No Hit
CCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC10770.1399810239280469No Hit
CTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGCT9050.11762565149014154Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9010.11710575910786467No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT8680.1128166469540805No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC8580.11151691599838834No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC8260.10735777694017337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG253.8895985E-545.01
TATTCGA207.031832E-445.018
CGTTTTT79950.043.9305841
TTAGGCG556.002665E-1140.9090921
CCATACG556.002665E-1140.9090922
TGCGTAG451.9268555E-840.01
CGTTCTG3400.039.7058831
CGTATGG1150.039.1304322
TGCTACG356.2455947E-638.571431
CGTTTTC3150.038.571431
GTTTTTT91100.038.479692
CGTAAGG1000.038.252
CGTTAGG659.094947E-1238.076922
GCGTAAG659.094947E-1238.076921
CGGTCTA601.546141E-1037.49999631
CCGAACC601.546141E-1037.49999618
CGTTTCT4050.037.222221
AGGGCGA4900.037.193886
TAGCGCG504.8754373E-836.01
GCGTTAG504.8754373E-836.01
Produced by FastQC (version 0.11.5)