##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769390
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85086627068197	33.0	31.0	34.0	30.0	34.0
2	31.915000194959642	33.0	31.0	34.0	30.0	34.0
3	31.967398848438375	33.0	31.0	34.0	30.0	34.0
4	35.6940511314158	37.0	35.0	37.0	33.0	37.0
5	35.71295051924252	37.0	35.0	37.0	35.0	37.0
6	35.65734283003418	37.0	35.0	37.0	33.0	37.0
7	35.937911852246586	37.0	35.0	37.0	35.0	37.0
8	35.77687908602919	37.0	35.0	37.0	35.0	37.0
9	37.60157917311117	39.0	37.0	39.0	35.0	39.0
10	37.045972783633786	39.0	37.0	39.0	33.0	39.0
11	36.98173748034157	39.0	37.0	39.0	33.0	39.0
12	37.044598968013624	39.0	37.0	39.0	33.0	39.0
13	37.028570685868026	39.0	37.0	39.0	33.0	39.0
14	38.03273892304293	40.0	37.0	41.0	33.0	41.0
15	38.13013556193868	40.0	37.0	41.0	33.0	41.0
16	38.079793082831856	40.0	37.0	41.0	33.0	41.0
17	38.063331990278016	40.0	37.0	41.0	33.0	41.0
18	37.84457557285642	39.0	37.0	41.0	33.0	41.0
19	37.60474791718114	39.0	37.0	41.0	33.0	41.0
20	37.49468669985313	39.0	35.0	41.0	33.0	41.0
21	37.44617814112479	39.0	35.0	41.0	33.0	41.0
22	37.57713643275842	39.0	35.0	41.0	33.0	41.0
23	37.599377428872224	39.0	35.0	41.0	33.0	41.0
24	37.572047986066885	39.0	35.0	41.0	33.0	41.0
25	37.3088706637726	39.0	35.0	41.0	33.0	41.0
26	37.37203498875733	39.0	35.0	41.0	33.0	41.0
27	37.385346833205524	39.0	35.0	41.0	33.0	41.0
28	37.21041474414796	39.0	35.0	41.0	33.0	41.0
29	37.145451591520555	39.0	35.0	41.0	33.0	41.0
30	36.97194400759043	39.0	35.0	41.0	32.0	41.0
31	36.86480588518177	39.0	35.0	41.0	31.0	41.0
32	36.670110087211945	39.0	35.0	41.0	31.0	41.0
33	36.300233951572025	39.0	35.0	41.0	30.0	41.0
34	36.01345611458428	39.0	35.0	41.0	29.0	41.0
35	35.83388138655298	39.0	35.0	41.0	27.0	41.0
36	35.69221591130636	39.0	35.0	41.0	25.0	41.0
37	35.63645875303812	39.0	35.0	41.0	25.0	41.0
38	35.507385071290244	39.0	35.0	41.0	24.0	41.0
39	35.42234497459026	39.0	35.0	41.0	24.0	41.0
40	35.27539999220161	39.0	35.0	41.0	23.0	41.0
41	35.09952819766308	39.0	35.0	41.0	22.0	41.0
42	35.153747774210736	39.0	35.0	41.0	23.0	41.0
43	35.10572791432173	39.0	35.0	41.0	23.0	41.0
44	34.9973043579979	39.0	35.0	41.0	22.0	41.0
45	34.983230871209656	39.0	35.0	40.0	23.0	41.0
46	34.91294921951156	38.0	35.0	40.0	23.0	41.0
47	34.847832698631386	38.0	34.0	40.0	22.0	41.0
48	34.755076099247454	38.0	34.0	40.0	22.0	41.0
49	34.698090695226085	38.0	34.0	40.0	22.0	41.0
50	34.59244596368552	38.0	34.0	40.0	21.0	41.0
51	33.457913411923734	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	4.0
10	11.0
11	10.0
12	8.0
13	18.0
14	22.0
15	33.0
16	66.0
17	131.0
18	287.0
19	492.0
20	918.0
21	1453.0
22	2163.0
23	3248.0
24	5191.0
25	8896.0
26	14032.0
27	17055.0
28	16931.0
29	15509.0
30	15689.0
31	17326.0
32	21446.0
33	29062.0
34	45422.0
35	49904.0
36	58939.0
37	83131.0
38	151839.0
39	210056.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.00983896333459	19.531446990472972	22.583215274438192	20.87549877175425
2	37.889106954860345	24.461976370891225	22.916596264573233	14.732320409675198
3	27.27381432043567	23.836545835012153	34.39179089928385	14.497848945268329
4	24.33200327532201	22.497692977553648	36.78615526585997	16.38414848126438
5	24.10585008903157	25.84761954275465	32.688623454944825	17.357906913268952
6	23.073993683307556	30.493507843876316	32.42087887807224	14.011619594743888
7	71.62232417889497	4.669543404515266	19.111503918688832	4.596628497900934
8	73.11532512769857	5.283406334888678	17.01815724145102	4.583111295961736
9	66.80149209113713	6.389867297469423	18.9110854053211	7.897555206072343
10	34.40231872002496	28.275646941083192	24.44949895371658	12.872535385175269
11	24.559716138759278	21.71876421580733	35.592872275438985	18.12864736999441
12	22.672766737285382	19.275919884583892	37.943695654999416	20.107617723131312
13	22.177569243166666	19.86222851869663	39.94333173033182	18.016870507804885
14	17.97618892889172	24.43624169796852	38.07574832009774	19.51182105304202
15	17.15098974512276	23.954041513406725	40.895644601567476	17.999324139903038
16	21.512496913138982	24.489140747865193	35.61587751335474	18.382484825641093
17	21.02743732047466	24.757665163311195	34.725431835610024	19.489465680604116
18	21.043164065038535	24.126645784322644	35.5020210816361	19.328169069002715
19	20.49831684841238	26.407803584657973	33.0134262207723	20.080453346157345
20	20.5248313599085	26.910149599033	33.890094750386666	18.674924290671832
21	20.975448082246974	26.087939796462134	35.53100508194804	17.405607039342854
22	19.713149378078736	22.68927332042267	36.55246364002652	21.045113661472076
23	18.994268186485396	24.48433174332913	36.27432121550839	20.24707885467708
24	20.191970262155735	24.27689468280066	35.154343050988444	20.37679200405516
25	20.92839782165092	26.201926201276336	32.65963945463289	20.210036522439857
26	19.1217717932388	25.084547498667774	32.983012516409104	22.81066819168432
27	20.063426870637777	24.164987847515565	33.45780423452345	22.313781047323204
28	18.959955289255124	24.026826446925487	33.95833062556051	23.05488763825888
29	19.701451799477507	23.27766152406452	32.774665644211645	24.246221032246325
30	20.037822170810642	23.09985832932583	33.41374335512549	23.44857614473804
31	20.176503463782996	22.674326414432215	33.56815139266172	23.58101872912307
32	21.10685088186745	23.60597356347236	32.088017780319475	23.199157774340712
33	20.072005094945347	24.69358842719557	31.71187564174216	23.52253083611692
34	19.22951948946568	24.948595640702376	33.880476741314546	21.9414081285174
35	19.804910383550606	25.89272020691717	31.609196896242477	22.69317251328975
36	20.541857835428065	27.61967272774536	30.74084664474454	21.09762279208204
37	19.4988237434851	27.58120069145687	31.632332107253795	21.287643457804233
38	20.390439179089928	27.13305345793421	30.730968689481276	21.745538673494586
39	20.70549396274971	25.80160906692315	30.832737623311974	22.660159347015167
40	22.334576742614278	25.272748541052003	31.752167301368615	20.640507414965104
41	19.69287357516994	26.703752323269082	31.361988068469827	22.24138603309115
42	20.532759718738223	26.457323334069848	31.939718478274997	21.070198468916935
43	20.900713552294675	25.640052509130612	31.50924758574975	21.949986352824965
44	21.40162986261844	24.913502904898685	30.51079426558702	23.174072966895853
45	20.10085912216171	24.394130415004096	30.231092163922067	25.273918298912122
46	21.673663551644808	24.684750256696862	30.20106837884558	23.440517812812747
47	19.64465355671376	24.197741067599008	33.17706234809394	22.98054302759329
48	19.555881932439988	24.67383251666905	32.03771819233418	23.73256735855678
49	20.30205747410286	23.34264807184913	33.12039407842576	23.234900375622246
50	19.01181455438724	23.351486242347836	34.039953729577974	23.596745473686948
51	18.901597369344543	23.183690975967973	32.51354969521309	25.40116195947439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	587.5
2	786.0
3	12374.5
4	23963.0
5	15874.5
6	7786.0
7	7523.0
8	7260.0
9	7308.0
10	7356.0
11	7164.5
12	6973.0
13	6683.5
14	6394.0
15	5979.5
16	5565.0
17	5206.0
18	4847.0
19	4623.0
20	4399.0
21	4175.5
22	3952.0
23	4293.5
24	4635.0
25	5039.5
26	6222.5
27	7001.0
28	8475.0
29	9949.0
30	11046.0
31	12143.0
32	13753.5
33	15364.0
34	17559.5
35	19755.0
36	21751.0
37	23747.0
38	25730.0
39	27713.0
40	29613.5
41	31514.0
42	34331.5
43	37149.0
44	41023.0
45	44897.0
46	64134.0
47	83371.0
48	78451.0
49	73531.0
50	70711.5
51	67892.0
52	59186.0
53	50480.0
54	44565.5
55	38651.0
56	36334.0
57	34017.0
58	31590.5
59	29164.0
60	25906.5
61	22649.0
62	20074.0
63	17499.0
64	15001.0
65	12503.0
66	10134.0
67	7765.0
68	6373.5
69	4982.0
70	4132.0
71	3282.0
72	2578.0
73	1874.0
74	1639.0
75	1053.0
76	702.0
77	538.5
78	375.0
79	252.5
80	130.0
81	104.0
82	78.0
83	54.5
84	31.0
85	25.0
86	19.0
87	12.5
88	6.0
89	3.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	769390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.67712533021941
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9802487525891	28.559693510568472
2	8.837443892953658	7.012887378790087
3	4.254068809367933	5.063676639380063
4	2.7556524711250563	4.373454738534307
5	2.1586102010675514	4.282372374342367
6	1.7265421512368189	4.110253759351794
7	1.446992164763965	4.0188742631229735
8	1.1679626267064136	3.707311961667411
9	0.986990823650471	3.5244862748780594
>10	4.626295555671562	26.134519440860878
>50	0.03650209046265053	0.9601822115266972
>100	0.015784668107664736	1.1681618023441922
>500	0.0026307780179381625	0.8076534744221483
>1k	0.0036173197746649736	3.3600472170144045
>5k	3.288472522422703E-4	1.0328570118781464
>10k+	3.288472522422703E-4	1.8835679413179536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14436	1.8762916076372191	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	7916	1.028867024525923	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTT	3498	0.4546458883011217	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG	3442	0.44736739494924554	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC	3224	0.4190332601151561	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	2594	0.3371502099065493	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT	2310	0.3002378507648917	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTT	2206	0.2867206488256931	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT	1966	0.25552710588908095	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCT	1826	0.23733087250939056	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1819	0.23642106084040604	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	1790	0.23265184106889872	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	1077	0.1399810239280469	No Hit
CTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGCT	905	0.11762565149014154	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	901	0.11710575910786467	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCT	868	0.1128166469540805	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC	858	0.11151691599838834	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTC	826	0.10735777694017337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.899192867076515E-4	0.0	0.0	0.12698371437112518	0.0
2	3.899192867076515E-4	0.0	0.0	0.5891680422152614	0.0
3	3.899192867076515E-4	0.0	0.0	1.0421242802739832	0.0
4	3.899192867076515E-4	0.0	0.0	1.8699229259543275	0.0
5	3.899192867076515E-4	0.0	0.0	4.0055108592521345	0.0
6	3.899192867076515E-4	0.0	0.0	5.924433642236057	0.0
7	3.899192867076515E-4	0.0	0.0	7.271604777810993	0.0
8	3.899192867076515E-4	0.0	0.0	8.849998050403567	0.0
9	3.899192867076515E-4	0.0	0.0	9.782684984208268	0.0
10	5.198923822768687E-4	0.0	0.0	11.339632696031922	0.0
11	5.198923822768687E-4	0.0	0.0	12.31625053613902	0.0
12	5.198923822768687E-4	0.0	0.0	13.501085275348004	0.0
13	6.498654778460858E-4	0.0	0.0	13.996932634944567	0.0
14	6.498654778460858E-4	0.0	0.0	14.348899777746007	0.0
15	6.498654778460858E-4	0.0	0.0	14.661225126398836	0.0
16	0.0027294350069535607	0.0	0.0	15.004094152510431	0.0
17	0.0027294350069535607	0.0	0.0	15.398562497563004	0.0
18	0.0027294350069535607	0.0	0.0	15.855547901584371	0.0
19	0.0027294350069535607	0.0	0.0	16.19620738507129	0.0
20	0.0027294350069535607	0.0	0.0	16.528808536632916	0.0
21	0.0027294350069535607	0.0	0.0	16.87557675561159	0.0
22	0.002859408102522778	0.0	0.0	17.267835558039486	0.0
23	0.002859408102522778	0.0	0.0	17.624221786090278	0.0
24	0.002859408102522778	0.0	0.0	17.91978060541468	0.0
25	0.002859408102522778	0.0	0.0	18.222357971899818	0.0
26	0.002859408102522778	0.0	0.0	18.540272163662124	0.0
27	0.002859408102522778	0.0	0.0	18.85597681279975	0.0
28	0.002859408102522778	0.0	0.0	19.14334732710329	0.0
29	0.002859408102522778	0.0	0.0	19.458272137667503	0.0
30	0.002859408102522778	0.0	0.0	19.88003483278961	0.0
31	0.002989381198091995	0.0	0.0	20.20587738338164	0.0
32	0.002989381198091995	0.0	0.0	20.550696005926774	0.0
33	0.003119354293661212	0.0	0.0	20.902143256345937	0.0
34	0.003119354293661212	0.0	0.0	21.27035703609353	0.0
35	0.003119354293661212	0.0	0.0	21.64221006251706	0.0
36	0.003119354293661212	0.0	0.0	21.984689169341948	0.0
37	0.003119354293661212	0.0	0.0	22.330157657364925	0.0
38	0.003119354293661212	0.0	0.0	22.738273177452268	0.0
39	0.0032493273892304293	0.0	0.0	23.319382887742236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	25	3.8895985E-5	45.0	1
TATTCGA	20	7.031832E-4	45.0	18
CGTTTTT	7995	0.0	43.930584	1
TTAGGCG	55	6.002665E-11	40.909092	1
CCATACG	55	6.002665E-11	40.909092	2
TGCGTAG	45	1.9268555E-8	40.0	1
CGTTCTG	340	0.0	39.705883	1
CGTATGG	115	0.0	39.130432	2
TGCTACG	35	6.2455947E-6	38.57143	1
CGTTTTC	315	0.0	38.57143	1
GTTTTTT	9110	0.0	38.47969	2
CGTAAGG	100	0.0	38.25	2
CGTTAGG	65	9.094947E-12	38.07692	2
GCGTAAG	65	9.094947E-12	38.07692	1
CGGTCTA	60	1.546141E-10	37.499996	31
CCGAACC	60	1.546141E-10	37.499996	18
CGTTTCT	405	0.0	37.22222	1
AGGGCGA	490	0.0	37.19388	6
TAGCGCG	50	4.8754373E-8	36.0	1
GCGTTAG	50	4.8754373E-8	36.0	1
>>END_MODULE