SRR2935404.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458605
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	9680	2.110748901560166	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	8781	1.91471963890494	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	5465	1.1916573085770978	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2462	0.5368454334340009	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	1902	0.41473599284787566	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1698	0.37025326806292996	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	1652	0.36022284972906965	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	1306	0.28477665965264226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	1103	0.24051198744017183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1040	0.22677467537423274	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	747	0.16288527163899216	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	699	0.15241874816018142	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	576	0.1255982817457289	TruSeq Adapter, Index 21 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	7.028847E-4	45.000004	45
TGCGATG	20	7.028847E-4	45.000004	1
TATAGCG	20	7.028847E-4	45.000004	1
ACGTAAG	20	7.028847E-4	45.000004	1
ACGGGCC	25	3.8871265E-5	45.0	5
CGTAAGG	95	0.0	45.0	2
AATAGCG	25	3.8871265E-5	45.0	1
TCACGAC	30	2.1626383E-6	44.999996	25
CGACGGT	30	2.1626383E-6	44.999996	28
CACGACG	30	2.1626383E-6	44.999996	26
TACGGCT	1135	0.0	41.828194	7
CGATGAA	675	0.0	41.33333	19
GCTACGA	60	3.6379788E-12	41.249996	10
GCGTAAG	60	3.6379788E-12	41.249996	1
ACGGCTG	1160	0.0	40.926727	8
GATGAAT	765	0.0	40.588234	20
CGGCGGA	50	1.0786607E-9	40.5	31
CCGATGA	650	0.0	40.5	18
CGCGGGT	45	1.9239451E-8	40.0	4
CGGCTGT	1190	0.0	39.516808	9