##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458605
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75057838444849	31.0	31.0	34.0	30.0	34.0
2	31.811650548947352	33.0	31.0	34.0	30.0	34.0
3	31.91371005549438	33.0	31.0	34.0	30.0	34.0
4	35.6446898747288	37.0	35.0	37.0	33.0	37.0
5	35.637236837801595	37.0	35.0	37.0	33.0	37.0
6	35.56617786548337	37.0	35.0	37.0	33.0	37.0
7	35.83576280241166	37.0	35.0	37.0	35.0	37.0
8	35.5553362915799	37.0	35.0	37.0	33.0	37.0
9	37.3522704724109	39.0	37.0	39.0	34.0	39.0
10	36.766827662149346	39.0	37.0	39.0	32.0	39.0
11	36.79223078684271	39.0	35.0	39.0	32.0	39.0
12	36.804197512020146	39.0	35.0	39.0	33.0	39.0
13	36.70157324931041	39.0	35.0	39.0	32.0	39.0
14	37.727052692404136	40.0	36.0	41.0	33.0	41.0
15	37.7399286968088	40.0	36.0	41.0	33.0	41.0
16	37.80162885271639	40.0	36.0	41.0	33.0	41.0
17	37.67390019733758	39.0	36.0	41.0	32.0	41.0
18	37.67066429716205	39.0	36.0	41.0	33.0	41.0
19	37.59378550168446	39.0	36.0	41.0	32.0	41.0
20	37.44566675025349	39.0	36.0	41.0	32.0	41.0
21	37.57520960303529	39.0	36.0	41.0	32.0	41.0
22	37.658797876167945	39.0	36.0	41.0	33.0	41.0
23	37.61514157063268	39.0	36.0	41.0	33.0	41.0
24	37.61599633671678	39.0	36.0	41.0	33.0	41.0
25	37.46680912768068	39.0	35.0	41.0	33.0	41.0
26	37.43708638152658	39.0	36.0	41.0	33.0	41.0
27	37.403654561114685	39.0	36.0	41.0	32.0	41.0
28	37.287033503777764	39.0	35.0	41.0	32.0	41.0
29	37.27805409884323	39.0	35.0	41.0	32.0	41.0
30	37.17608835490237	39.0	35.0	41.0	32.0	41.0
31	37.26431896730302	39.0	35.0	41.0	32.0	41.0
32	37.020416262360854	39.0	35.0	41.0	31.0	41.0
33	36.96559130406341	39.0	35.0	41.0	31.0	41.0
34	36.83963105504737	39.0	35.0	41.0	31.0	41.0
35	36.601692087962405	39.0	35.0	41.0	30.0	41.0
36	36.70094307737595	39.0	35.0	41.0	31.0	41.0
37	36.58596177538404	39.0	35.0	41.0	30.0	41.0
38	36.59549939490411	39.0	35.0	41.0	30.0	41.0
39	36.53449046565127	39.0	35.0	41.0	31.0	41.0
40	36.31090371888662	39.0	35.0	41.0	30.0	41.0
41	36.23327700308544	39.0	35.0	41.0	30.0	41.0
42	36.12429432736233	39.0	35.0	41.0	29.0	41.0
43	36.191437075478895	39.0	35.0	41.0	30.0	41.0
44	36.204339246192255	39.0	35.0	41.0	30.0	41.0
45	36.236672081638886	39.0	35.0	41.0	30.0	41.0
46	36.10733855932666	39.0	35.0	40.0	29.0	41.0
47	35.900788260049495	38.0	35.0	40.0	29.0	41.0
48	35.905371725122926	38.0	35.0	40.0	29.0	41.0
49	35.811694159461844	38.0	35.0	40.0	29.0	41.0
50	35.66232378626487	38.0	35.0	40.0	28.0	41.0
51	34.44813510537391	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	2.0
11	7.0
12	9.0
13	6.0
14	19.0
15	20.0
16	27.0
17	61.0
18	96.0
19	341.0
20	702.0
21	1502.0
22	2238.0
23	2843.0
24	2941.0
25	3319.0
26	3866.0
27	4573.0
28	5116.0
29	5846.0
30	7511.0
31	9514.0
32	13136.0
33	18239.0
34	30664.0
35	36190.0
36	35955.0
37	53294.0
38	93899.0
39	126626.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.43124257258425	19.642829886285583	23.60419097044297	10.321736570687193
2	27.785130995082913	32.48023898561943	24.17766923605281	15.556960783244842
3	28.70531285092836	32.04609631382126	24.71604103749414	14.53254979775624
4	25.65235878370275	23.608770074464953	34.091865548783815	16.647005593048483
5	29.968709455849808	27.58452262840571	23.712345046390684	18.7344228693538
6	23.528526727794073	39.54492428124421	23.73720303965286	13.189345951308862
7	74.47585612891268	5.732166025228683	15.45556633704386	4.336411508814775
8	74.32496374875983	11.814088376707623	8.98093130253704	4.880016571995508
9	68.47810207040918	7.570349211194818	9.841802858669226	14.10974585972678
10	41.311368170866	26.477687770521474	18.355447498391865	13.85549656022067
11	33.654234035826036	23.691848104578014	24.142344719311826	18.511573140284124
12	26.89700286739133	20.389223841868272	29.079273012723366	23.63450027801703
13	26.798879209777475	22.47576890788369	32.55394075511606	18.171411127222772
14	20.97927410298623	30.145768144699687	27.41509577959246	21.459861972721622
15	18.711309296671427	24.32986993164052	36.40322281702119	20.555597954666872
16	21.35672310594084	25.13317560863924	28.43318324047928	25.076918044940633
17	21.817468191580993	26.732809280317483	29.231909813456024	22.2178127146455
18	21.82117508531307	25.736090971533237	28.161707787747623	24.281026155406067
19	23.029840494543237	26.75897558901451	26.201851266340313	24.00933265010194
20	26.21885936699338	26.535689754799886	29.15450115022732	18.090949727979417
21	23.60855202189248	30.752608453898233	27.10131812780061	18.537521396408675
22	21.601378092258045	24.593931596908014	29.65427764634053	24.15041266449341
23	23.217583759444402	27.665420132794015	28.500997590519074	20.615998517242506
24	23.42822254445547	25.7980179021162	27.243270352481986	23.53048920094635
25	20.684903130144676	31.899783037690387	25.190087330055277	22.225226502109656
26	21.30068359481471	25.64014783964414	28.451499656567197	24.607668908973952
27	24.008678492384515	28.148842685971587	26.104817871588843	21.73766095005506
28	19.388580586779472	27.745445426892424	29.446473544771646	23.41950044155646
29	24.266198580477752	25.777302907731052	27.4798573936176	22.47664111817359
30	22.65980527905278	29.97437882273416	27.109822178127146	20.255993720085915
31	24.88873867489452	25.250051787485962	23.825732384077803	26.03547715354172
32	26.43211478287415	30.624829646427752	23.96397771502709	18.979077855671004
33	26.78775852858124	26.438002202330985	23.683562106823956	23.090677162263823
34	27.41596798988236	25.13186729320439	25.985979219589844	21.466185497323405
35	22.94610830671275	27.00297641761429	26.985096106671318	23.065819169001646
36	25.276872253900418	29.475692589483327	23.856477796796806	21.390957359819453
37	21.33164706010619	31.904580194284843	27.552250847679378	19.21152189792959
38	22.90402416022503	27.467428396986517	24.057958373763917	25.570589069024543
39	25.44041168325683	29.414419816617787	24.2869135748629	20.858254925262482
40	25.351228181114465	24.43846011273318	28.844212339595078	21.366099366557275
41	23.245058383576282	28.05835086839437	25.282323568212295	23.414267179817053
42	21.100075228137506	25.73085770979383	30.908297990645544	22.26076907142312
43	23.75879024432791	27.8706076034932	25.18616238375072	23.184439768428167
44	21.934126317855235	25.741106180700168	27.99533367494903	24.329433826495567
45	21.59374625222141	24.357780660917346	26.473326719071967	27.575146367789273
46	26.646460461617295	27.02914272631131	25.06536125859945	21.259035553471943
47	20.99650025621177	25.43779505238713	31.707896773912193	21.857807917488906
48	22.127975054785708	25.983362588720137	27.069918557364183	24.81874379912997
49	21.745728895236642	23.8732678448774	32.46955440956815	21.91144885031781
50	22.366524569073604	24.195767599568256	29.084506274462775	24.353201556895367
51	21.204522410353135	23.92494630455403	26.75766727357966	28.112864011513178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	288.5
2	456.0
3	1257.0
4	2058.0
5	1430.5
6	803.0
7	791.0
8	779.0
9	793.0
10	807.0
11	849.5
12	892.0
13	902.0
14	912.0
15	921.0
16	930.0
17	982.5
18	1035.0
19	1039.5
20	1044.0
21	1131.0
22	1218.0
23	1559.0
24	1900.0
25	2178.5
26	3106.5
27	3756.0
28	4385.0
29	5014.0
30	5997.5
31	6981.0
32	7950.5
33	8920.0
34	10892.5
35	12865.0
36	13775.0
37	14685.0
38	15468.0
39	16251.0
40	18159.0
41	20067.0
42	22182.5
43	24298.0
44	26528.5
45	28759.0
46	46812.5
47	64866.0
48	53487.5
49	42109.0
50	41423.5
51	40738.0
52	35913.0
53	31088.0
54	28146.0
55	25204.0
56	24127.5
57	23051.0
58	21424.5
59	19798.0
60	18876.0
61	17954.0
62	15974.0
63	13994.0
64	11044.5
65	8095.0
66	6945.0
67	5795.0
68	4737.5
69	3680.0
70	2981.5
71	2283.0
72	1775.0
73	1267.0
74	1051.5
75	607.0
76	378.0
77	322.0
78	266.0
79	181.0
80	96.0
81	74.5
82	53.0
83	42.5
84	32.0
85	21.0
86	10.0
87	6.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.11035063170482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47437038839405	24.32762166003477
2	8.438890181275672	5.588292256889772
3	3.4047674606383125	3.381991333234613
4	2.0259998784436055	2.683262654202365
5	1.5355203167003364	2.5420808044027283
6	1.2492922172025922	2.4818702011822658
7	0.9769103572987526	2.2642091126134014
8	0.9217193990548066	2.4414761989399123
9	0.7974938365228678	2.376477049850607
>10	7.089850294903756	41.34230038928214
>50	0.05745080040449214	1.2939442699840031
>100	0.019150266801497378	1.1623196585391955
>500	0.001981062082913522	0.4421012696701003
>1k	0.004622478193464884	2.440740095611934
>5k	0.001981062082913522	5.231313045562226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	9680	2.110748901560166	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	8781	1.91471963890494	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	5465	1.1916573085770978	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2462	0.5368454334340009	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	1902	0.41473599284787566	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1698	0.37025326806292996	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	1652	0.36022284972906965	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	1306	0.28477665965264226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	1103	0.24051198744017183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1040	0.22677467537423274	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	747	0.16288527163899216	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	699	0.15241874816018142	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	576	0.1255982817457289	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3610514495044753E-4	0.0	0.0	0.13737312065939097	0.0
2	4.3610514495044753E-4	0.0	0.0	0.9936655727695948	0.0
3	4.3610514495044753E-4	0.0	0.0	1.2439899259711515	0.0
4	4.3610514495044753E-4	0.0	0.0	1.8892074879253389	0.0
5	4.3610514495044753E-4	0.0	0.0	4.582156757994352	0.0
6	4.3610514495044753E-4	0.0	0.0	5.236096422847549	0.0
7	4.3610514495044753E-4	0.0	0.0	6.102201240719137	0.0
8	4.3610514495044753E-4	0.0	0.0	7.207727783168522	0.0
9	4.3610514495044753E-4	0.0	0.0	7.55704800427383	0.0
10	4.3610514495044753E-4	0.0	0.0	9.489429901549263	0.0
11	4.3610514495044753E-4	0.0	0.0	10.392385604169165	0.0
12	4.3610514495044753E-4	0.0	0.0	13.37338232247795	0.0
13	4.3610514495044753E-4	0.0	0.0	13.845248089314333	0.0
14	4.3610514495044753E-4	0.0	0.0	14.077256026427971	0.0
15	4.3610514495044753E-4	0.0	0.0	14.54803153040198	0.0
16	4.3610514495044753E-4	0.0	0.0	15.019897297238364	0.0
17	4.3610514495044753E-4	0.0	0.0	15.554780257520088	0.0
18	4.3610514495044753E-4	0.0	0.0	16.107761581317256	0.0
19	4.3610514495044753E-4	0.0	0.0	16.923932360092017	0.0
20	4.3610514495044753E-4	0.0	0.0	17.37944418399276	0.0
21	4.3610514495044753E-4	0.0	0.0	17.815113223798257	0.0
22	4.3610514495044753E-4	0.0	0.0	18.359808549841368	0.0
23	4.3610514495044753E-4	0.0	0.0	18.81946337261914	0.0
24	4.3610514495044753E-4	0.0	0.0	19.211303845357115	0.0
25	4.3610514495044753E-4	0.0	0.0	19.568037853926583	0.0
26	4.3610514495044753E-4	0.0	0.0	19.90776376184298	0.0
27	4.3610514495044753E-4	0.0	0.0	20.33601901418432	0.0
28	4.3610514495044753E-4	0.0	0.0	20.682286499274976	0.0
29	4.3610514495044753E-4	0.0	0.0	21.045998190163647	0.0
30	4.3610514495044753E-4	0.0	0.0	21.501291961491916	0.0
31	4.3610514495044753E-4	0.0	0.0	21.87961317473643	0.0
32	4.3610514495044753E-4	0.0	0.0	22.250956705661736	0.0
33	4.3610514495044753E-4	0.0	0.0	22.617066974847635	0.0
34	4.3610514495044753E-4	0.0	0.0	22.98797440062799	0.0
35	4.3610514495044753E-4	0.0	0.0	23.373491348764187	0.0
36	4.3610514495044753E-4	0.0	0.0	23.759662454617807	0.0
37	4.3610514495044753E-4	0.0	0.0	24.146487718188855	0.0
38	4.3610514495044753E-4	0.0	0.0	24.533967139477326	0.0
39	6.541577174256713E-4	0.0	0.0	24.98904285823312	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	7.028847E-4	45.000004	45
TGCGATG	20	7.028847E-4	45.000004	1
TATAGCG	20	7.028847E-4	45.000004	1
ACGTAAG	20	7.028847E-4	45.000004	1
ACGGGCC	25	3.8871265E-5	45.0	5
CGTAAGG	95	0.0	45.0	2
AATAGCG	25	3.8871265E-5	45.0	1
TCACGAC	30	2.1626383E-6	44.999996	25
CGACGGT	30	2.1626383E-6	44.999996	28
CACGACG	30	2.1626383E-6	44.999996	26
TACGGCT	1135	0.0	41.828194	7
CGATGAA	675	0.0	41.33333	19
GCTACGA	60	3.6379788E-12	41.249996	10
GCGTAAG	60	3.6379788E-12	41.249996	1
ACGGCTG	1160	0.0	40.926727	8
GATGAAT	765	0.0	40.588234	20
CGGCGGA	50	1.0786607E-9	40.5	31
CCGATGA	650	0.0	40.5	18
CGCGGGT	45	1.9239451E-8	40.0	4
CGGCTGT	1190	0.0	39.516808	9
>>END_MODULE