##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471745
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837204421880465	33.0	31.0	34.0	30.0	34.0
2	31.88253823569937	33.0	31.0	34.0	30.0	34.0
3	31.978746992548942	33.0	31.0	34.0	30.0	34.0
4	35.71798535225599	37.0	35.0	37.0	33.0	37.0
5	35.74035760845372	37.0	35.0	37.0	35.0	37.0
6	35.69365653054086	37.0	35.0	37.0	33.0	37.0
7	35.978193727543484	37.0	35.0	37.0	35.0	37.0
8	35.81049083721078	37.0	35.0	37.0	35.0	37.0
9	37.62574060138422	39.0	37.0	39.0	35.0	39.0
10	36.93963052072624	39.0	37.0	39.0	32.0	39.0
11	36.89825435351726	39.0	37.0	39.0	33.0	39.0
12	36.96141559528983	39.0	37.0	39.0	33.0	39.0
13	36.929095167940304	39.0	37.0	39.0	33.0	39.0
14	37.93560716064823	40.0	37.0	41.0	33.0	41.0
15	38.08314237564786	40.0	37.0	41.0	33.0	41.0
16	38.007122492024294	40.0	37.0	41.0	33.0	41.0
17	38.03425579497398	40.0	37.0	41.0	33.0	41.0
18	37.97952283543016	40.0	37.0	41.0	33.0	41.0
19	37.88481064982141	40.0	37.0	41.0	33.0	41.0
20	37.96125449130356	40.0	36.0	41.0	34.0	41.0
21	37.89129720505782	40.0	36.0	41.0	33.0	41.0
22	37.999815578331514	40.0	37.0	41.0	34.0	41.0
23	37.99577314014987	40.0	36.0	41.0	34.0	41.0
24	37.9950714898939	40.0	36.0	41.0	34.0	41.0
25	37.72839563747363	39.0	36.0	41.0	33.0	41.0
26	37.823578416305416	40.0	36.0	41.0	33.0	41.0
27	37.83768773383925	40.0	36.0	41.0	33.0	41.0
28	37.75421043148311	40.0	36.0	41.0	33.0	41.0
29	37.755719721459684	40.0	36.0	41.0	33.0	41.0
30	37.63271894773659	40.0	36.0	41.0	33.0	41.0
31	37.60177426363819	40.0	36.0	41.0	33.0	41.0
32	37.57951647606228	40.0	36.0	41.0	33.0	41.0
33	37.488058167018195	40.0	36.0	41.0	33.0	41.0
34	37.437823400354006	40.0	36.0	41.0	33.0	41.0
35	37.378176769229135	40.0	36.0	41.0	33.0	41.0
36	37.29168088691984	40.0	36.0	41.0	33.0	41.0
37	37.2541394185418	40.0	36.0	41.0	32.0	41.0
38	37.16849145194968	40.0	35.0	41.0	32.0	41.0
39	37.129813776510616	40.0	35.0	41.0	32.0	41.0
40	37.03099131946284	40.0	35.0	41.0	32.0	41.0
41	36.91247601988363	39.0	35.0	41.0	31.0	41.0
42	36.93970047377291	39.0	35.0	41.0	32.0	41.0
43	36.87114860782838	39.0	35.0	41.0	31.0	41.0
44	36.7374195804937	39.0	35.0	41.0	31.0	41.0
45	36.7130822796214	39.0	35.0	41.0	31.0	41.0
46	36.60705253897763	39.0	35.0	41.0	31.0	41.0
47	36.55227506385865	39.0	35.0	41.0	31.0	41.0
48	36.48612067960445	39.0	35.0	41.0	31.0	41.0
49	36.41423014552353	39.0	35.0	41.0	31.0	41.0
50	36.284446046063024	39.0	35.0	41.0	31.0	41.0
51	35.08673329871011	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	1.0
11	3.0
12	7.0
13	4.0
14	5.0
15	22.0
16	26.0
17	44.0
18	116.0
19	201.0
20	348.0
21	533.0
22	828.0
23	1214.0
24	1815.0
25	2638.0
26	3694.0
27	4422.0
28	4902.0
29	5598.0
30	6990.0
31	8797.0
32	11613.0
33	16423.0
34	28765.0
35	34805.0
36	36226.0
37	53984.0
38	100791.0
39	146872.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.388981335255274	22.028426374418384	26.50414948754094	11.078442802785403
2	31.40277056460588	25.765614897879153	25.878811646122372	16.952802891392597
3	31.076323013492456	24.77736912950853	27.959808795005774	16.18649906199324
4	27.908085936257937	26.454334439156746	27.523980116376435	18.113599508208882
5	24.825064388599774	30.216536476274257	26.17643006285175	18.781969072274215
6	25.27149201369384	35.01595141442941	25.12565051033927	14.586906061537483
7	79.87790013672641	6.2525304984684515	9.294852091702085	4.574717273103054
8	81.2288418531198	5.72682275381827	8.37168385462485	4.67265153843708
9	75.08124092465209	7.991393655470647	10.559942341731233	6.367423078146032
10	38.529714146413845	30.381456083265324	17.498648634325747	13.590181135995083
11	28.297915187230387	26.59932802679414	27.1294873289595	17.973269457015974
12	26.95608856479666	23.044865340385165	30.507795525124802	19.49125056969337
13	24.55457927482008	25.58310103975665	31.050461584118537	18.81185810130473
14	20.502814020286383	28.661035093111746	30.023847629545624	20.81230325705625
15	19.209318593731783	27.319632428536604	35.037361286288146	18.43368769144347
16	22.475701915229624	26.959904185523957	31.181464562422494	19.38292933682392
17	22.862775440121254	26.585973354248587	28.760029253092245	21.791221952537917
18	23.865647754613192	25.98861673149689	30.24070207421382	19.905033439676096
19	23.639042279197447	28.43336972304953	28.226478288058164	19.701109709694855
20	24.26671188883825	27.82011468060075	28.683504859616953	19.229668570944046
21	23.715566672672736	28.746674580546696	28.86135518129498	18.676403565485593
22	22.303998982501138	26.50520938218741	28.737983444445618	22.452808190865827
23	20.83774072857158	28.344974509533756	29.00592481107378	21.81135995082089
24	21.163340363967823	26.696838334269575	30.428939363427276	21.71088193833533
25	20.99608898875452	29.151554335499053	27.752069444297238	22.100287231449194
26	20.75061738863157	28.30936204941229	28.814931795779497	22.125088766176642
27	21.13705497673531	28.14571431599699	29.084568993842012	21.632661713425687
28	19.863697548463684	28.79860941822383	29.43814984790512	21.89954318540737
29	22.26626673308673	26.72015601649196	28.74540270697093	22.26817454345038
30	22.619423629291248	27.332563143223567	27.930979660621734	22.117033566863455
31	22.95201856935421	28.35239377205906	26.70764925966359	21.987938398923145
32	24.58701205100213	28.591717983232467	25.837687733839253	20.983582231926146
33	22.632142365048914	27.76182047504478	26.663345663441053	22.94269149646525
34	21.577123233950545	27.90829791518723	28.58896225715164	21.925616593710583
35	21.29964281550414	27.56022851328578	27.974647320056384	23.165481351153698
36	21.61803516730437	29.282345334873717	27.183329977000287	21.91628952082163
37	21.062014435765086	29.76862499867513	28.54381074521193	20.625549820347857
38	20.922320321360058	29.83497440354429	27.01947026465569	22.22323501043996
39	21.787618310739912	27.045967630817497	28.875769748487002	22.29064430995559
40	22.323077086137637	26.42698915727777	29.616424127441732	21.633509629142864
41	20.019926019353676	27.237808561828952	29.16427307125672	23.577992347560652
42	21.483428547202408	27.366055814052082	29.177415764872972	21.97309987387254
43	21.702614760092846	26.277120054266607	29.072274215942933	22.947990969697614
44	21.20170855017011	27.476284857285187	28.09081177330973	23.231194819234968
45	21.37298752504001	26.576646281359633	27.630605517811528	24.419760675788826
46	22.04538468876194	27.419262525304983	27.569555586174733	22.965797199758345
47	20.150928997657633	26.39582825467149	30.58177617144856	22.871466576222325
48	20.64378000826718	26.42995686228789	29.694856331280672	23.23140679816426
49	21.50462644013185	25.14176090896565	30.140012082798968	23.21360056810353
50	20.443883877942532	25.70710871339389	30.011552851646545	23.837454557017033
51	19.694326383957435	25.64309107674697	28.35726928743283	26.305313251862767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	330.5
2	492.0
3	1734.5
4	2977.0
5	2010.5
6	1044.0
7	1057.0
8	1070.0
9	1143.5
10	1217.0
11	1326.0
12	1435.0
13	1435.5
14	1436.0
15	1464.0
16	1492.0
17	1437.5
18	1383.0
19	1359.0
20	1335.0
21	1589.0
22	1843.0
23	2192.5
24	2542.0
25	2957.5
26	3943.0
27	4513.0
28	5449.5
29	6386.0
30	7704.5
31	9023.0
32	10543.5
33	12064.0
34	13844.0
35	15624.0
36	16659.5
37	17695.0
38	19245.5
39	20796.0
40	22314.0
41	23832.0
42	26871.5
43	29911.0
44	31861.5
45	33812.0
46	39045.0
47	44278.0
48	46151.5
49	48025.0
50	46565.5
51	45106.0
52	39158.5
53	33211.0
54	29073.0
55	24935.0
56	22525.5
57	20116.0
58	18822.0
59	17528.0
60	15649.5
61	13771.0
62	12233.5
63	10696.0
64	8977.5
65	7259.0
66	5775.5
67	4292.0
68	3502.5
69	2713.0
70	2249.0
71	1785.0
72	1460.5
73	1136.0
74	840.5
75	491.5
76	438.0
77	327.5
78	217.0
79	154.5
80	92.0
81	93.0
82	94.0
83	55.5
84	17.0
85	15.0
86	13.0
87	9.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	471745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.72595797159395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02185210366707	27.1852342933882
2	9.203283778903	6.759988265292878
3	3.407233172401556	3.7540170686712075
4	1.9984854790766975	2.935851748456463
5	1.3894569574979099	2.5514568912203517
6	1.086014329046434	2.393094996306488
7	0.8919983672611508	2.2931646182734413
8	0.7633738794146175	2.2428509609595095
9	0.6753956902175301	2.232409835981222
>10	6.484812204716002	43.71777762624164
>50	0.0584259097599746	1.4508227446041575
>100	0.014461858851478855	0.8619106567373063
>500	0.0040493204784140795	1.0242226463225421
>1k	0.0011569487081183084	0.597197647544631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1742	0.3692672948308938	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	1069	0.22660547541574366	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	850	0.18018208990026394	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	750	0.15898419697082108	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	744	0.15771232339505453	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	743	0.1575003444657601	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	707	0.1498691030111607	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	518	0.10980508537451376	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	509	0.10789727501086391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.479157171777125E-4	0.0	0.0	0.16385971234459296	0.0
2	8.479157171777125E-4	0.0	0.0	0.7694835133387741	0.0
3	8.479157171777125E-4	0.0	0.0	1.1283638406342409	0.0
4	8.479157171777125E-4	0.0	0.0	1.5569852356675746	0.0
5	8.479157171777125E-4	0.0	0.0	2.7243531992919903	0.0
6	8.479157171777125E-4	0.0	0.0	3.59558659869209	0.0
7	0.0010598946464721406	0.0	0.0	4.393263309627023	0.0
8	0.0010598946464721406	0.0	0.0	5.767522708242801	0.0
9	0.0010598946464721406	0.0	0.0	6.3538564266711886	0.0
10	0.0010598946464721406	0.0	0.0	7.583546195508166	0.0
11	0.0010598946464721406	0.0	0.0	8.882765053153717	0.0
12	0.0010598946464721406	0.0	0.0	10.036566365303289	0.0
13	0.0012718735757665687	0.0	0.0	10.497832515447964	0.0
14	0.0014838525050609968	0.0	0.0	10.703240097934266	0.0
15	0.0014838525050609968	0.0	0.0	10.998102788582814	0.0
16	0.0014838525050609968	0.0	0.0	11.55751518299081	0.0
17	0.0014838525050609968	0.0	0.0	12.285662805117171	0.0
18	0.0014838525050609968	0.0	0.0	13.09224263108247	0.0
19	0.0014838525050609968	0.0	0.0	13.59632852494462	0.0
20	0.0014838525050609968	0.0	0.0	14.076672778725795	0.0
21	0.0014838525050609968	0.0	0.0	14.644352351376273	0.0
22	0.0014838525050609968	0.0	0.0	15.25357979416846	0.0
23	0.0014838525050609968	0.0	0.0	15.855387974435342	0.0
24	0.0014838525050609968	0.0	0.0	16.330856712842742	0.0
25	0.0014838525050609968	0.0	0.0	16.770924970057976	0.0
26	0.0014838525050609968	0.0	0.0	17.18555575575788	0.0
27	0.0014838525050609968	0.0	0.0	17.6069698671952	0.0
28	0.0014838525050609968	0.0	0.0	18.021600652895103	0.0
29	0.0014838525050609968	0.0	0.0	18.479899098029655	0.0
30	0.0014838525050609968	0.0	0.0	19.02044536773045	0.0
31	0.0014838525050609968	0.0	0.0	19.480439644299356	0.0
32	0.001695831434355425	0.0	0.0	19.94255371016121	0.0
33	0.001695831434355425	0.0	0.0	20.384105819881505	0.0
34	0.001695831434355425	0.0	0.0	20.841768328228174	0.0
35	0.001695831434355425	0.0	0.0	21.3125735301911	0.0
36	0.001695831434355425	0.0	0.0	21.741830862012318	0.0
37	0.001695831434355425	0.0	0.0	22.200977222864047	0.0
38	0.001695831434355425	0.0	0.0	22.646132974382347	0.0
39	0.001695831434355425	0.0	0.0	23.22271566206319	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGCG	20	7.029051E-4	45.000004	1
GCGAAAG	30	2.1627693E-6	45.000004	1
AAGCGCT	20	7.029051E-4	45.000004	42
ACGGGTC	40	6.8012014E-9	45.000004	5
GTCGTCC	20	7.029051E-4	45.000004	37
GCGATAC	20	7.029051E-4	45.000004	9
TTACGAG	20	7.029051E-4	45.000004	1
GCGTAAG	20	7.029051E-4	45.000004	1
AAGTCGC	20	7.029051E-4	45.000004	13
GCTAACG	25	3.8872968E-5	45.0	1
CTACGAA	50	1.0786607E-9	40.5	11
CGTTTTT	1025	0.0	40.17073	1
ATAGCGG	45	1.924127E-8	40.0	2
TTGCGAG	45	1.924127E-8	40.0	1
CACAACG	85	0.0	39.705883	12
CGTATGG	40	3.452442E-7	39.375004	2
ATTACGG	40	3.452442E-7	39.375004	2
TACCGGG	80	0.0	39.375004	3
CTCACGG	35	6.240458E-6	38.57143	2
CGAAAGG	70	0.0	38.57143	2
>>END_MODULE