##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290936
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983659636483626	33.0	31.0	34.0	30.0	34.0
2	32.02986567492507	33.0	31.0	34.0	30.0	34.0
3	32.09952360656639	33.0	31.0	34.0	30.0	34.0
4	35.81047378117524	37.0	35.0	37.0	35.0	37.0
5	35.836053290070666	37.0	35.0	37.0	35.0	37.0
6	35.80703316193252	37.0	35.0	37.0	35.0	37.0
7	36.04038345203069	37.0	35.0	37.0	35.0	37.0
8	35.91175378777463	37.0	35.0	37.0	35.0	37.0
9	37.76161767536503	39.0	38.0	39.0	35.0	39.0
10	37.15602400527951	39.0	37.0	39.0	33.0	39.0
11	37.11024761459565	39.0	37.0	39.0	34.0	39.0
12	37.1361227211483	39.0	37.0	39.0	34.0	39.0
13	37.066540407512306	39.0	37.0	39.0	33.0	39.0
14	38.09111282206396	40.0	37.0	41.0	33.0	41.0
15	38.22077364093821	40.0	37.0	41.0	33.0	41.0
16	38.19428327879671	40.0	37.0	41.0	34.0	41.0
17	38.201597602221796	40.0	37.0	41.0	34.0	41.0
18	38.12007795528913	40.0	37.0	41.0	34.0	41.0
19	38.00495985371353	40.0	37.0	41.0	34.0	41.0
20	37.98905601231886	40.0	36.0	41.0	34.0	41.0
21	37.96154824428741	40.0	36.0	41.0	34.0	41.0
22	38.0724557978387	40.0	37.0	41.0	34.0	41.0
23	38.084042538565186	40.0	37.0	41.0	34.0	41.0
24	38.08355789589463	40.0	36.0	41.0	34.0	41.0
25	37.82563175406275	39.0	36.0	41.0	34.0	41.0
26	37.92479101933071	40.0	36.0	41.0	34.0	41.0
27	37.91834974014903	40.0	36.0	41.0	34.0	41.0
28	37.76188921274782	40.0	36.0	41.0	34.0	41.0
29	37.685339731074876	40.0	36.0	41.0	33.0	41.0
30	37.55780996507823	40.0	36.0	41.0	33.0	41.0
31	37.564357796903785	40.0	36.0	41.0	33.0	41.0
32	37.51577666565843	40.0	36.0	41.0	33.0	41.0
33	37.34821060301922	40.0	36.0	41.0	33.0	41.0
34	37.25206230923639	40.0	36.0	41.0	33.0	41.0
35	37.15561154343223	40.0	36.0	41.0	32.0	41.0
36	37.031962355982074	40.0	35.0	41.0	32.0	41.0
37	36.96951219512195	40.0	35.0	41.0	32.0	41.0
38	36.851826518547036	40.0	35.0	41.0	31.0	41.0
39	36.75782302637006	39.0	35.0	41.0	31.0	41.0
40	36.653655786839714	39.0	35.0	41.0	31.0	41.0
41	36.49246913410509	39.0	35.0	41.0	30.0	41.0
42	36.52568949872137	39.0	35.0	41.0	31.0	41.0
43	36.44209723100613	39.0	35.0	41.0	30.0	41.0
44	36.34989482222895	39.0	35.0	41.0	30.0	41.0
45	36.33105906453653	39.0	35.0	41.0	30.0	41.0
46	36.196342150851045	39.0	35.0	41.0	30.0	41.0
47	36.14400417961338	39.0	35.0	41.0	30.0	41.0
48	36.05387439161878	39.0	35.0	41.0	29.0	41.0
49	35.905456870239504	39.0	35.0	40.0	29.0	41.0
50	35.73710025572635	39.0	35.0	40.0	28.0	41.0
51	34.43372425550636	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	2.0
11	3.0
12	1.0
13	6.0
14	5.0
15	7.0
16	18.0
17	29.0
18	73.0
19	135.0
20	225.0
21	421.0
22	717.0
23	1017.0
24	1432.0
25	2171.0
26	2946.0
27	3281.0
28	3262.0
29	3327.0
30	3967.0
31	4984.0
32	6366.0
33	9627.0
34	17036.0
35	21018.0
36	22078.0
37	34442.0
38	65152.0
39	87150.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.42975774740837	22.417645117826602	27.362375230291196	11.790221904473837
2	30.932576236698107	27.294662743696207	25.990939588088104	15.781821431517585
3	28.472241317678115	26.570448483515275	29.905202518767016	15.052107680039597
4	25.288723293095387	25.707028349877636	31.952044435889682	17.052203921137295
5	25.638628426870515	28.678128523111614	27.864547529353533	17.81869552066434
6	23.93275497016526	34.993606841367175	26.838548684246703	14.235089504220861
7	78.37978112024638	5.1516484725162925	12.077226606538895	4.3913438006984356
8	79.77527703687409	5.962136002419776	9.422003464679516	4.840583496026618
9	73.34327824676218	7.425688123848544	11.439973052492643	7.791060576896637
10	36.67748233288421	32.95948249786895	17.400046745676025	12.96298842357082
11	27.089119256468774	25.997126515797287	28.904638820909067	18.00911540682487
12	25.291473038743916	21.439079385156873	33.63385761817032	19.635589957928893
13	24.149984876398932	24.348653999505046	34.0830973134985	17.41826381059752
14	19.08804685566585	29.85880056094811	30.963854593450108	20.089297989935933
15	17.46810295047708	25.87441911623175	37.38726042841037	19.270217504880797
16	20.820042896032117	26.792146726427806	30.935669700552697	21.452140676987376
17	21.470014023702806	25.8981356724503	29.040063794099048	23.59178650974785
18	22.913630489179752	25.600475705997194	31.116465476943382	20.36942832787967
19	22.59637858498089	28.01234635796189	28.246418456292794	21.14485660076443
20	22.384304451838204	27.415651552231417	29.552891357549427	20.647152638380952
21	22.39392856160805	28.811147468859133	30.198737866747326	18.59618610278549
22	21.245909753347817	25.239915307834025	29.240451508235488	24.27372343058267
23	19.367146038991393	28.016814694640747	28.99744273654687	23.618596529820994
24	20.639247119641432	26.041466164379795	29.636071163417384	23.683215552561386
25	19.828072153325817	29.693815822036463	27.843237000577446	22.634875024060275
26	19.23275222041961	27.626350812549838	28.946916160255178	24.19398080677537
27	19.952498143921687	28.37084444688866	28.58601204388594	23.090645365303708
28	18.519880661038854	26.576635411224466	32.57761157092969	22.325872356806993
29	19.53934886023043	25.04399593037644	31.81730689911183	23.5993483102813
30	21.82507493056892	27.985880056094814	29.508895427172988	20.680149586163278
31	21.5518189567465	27.906137432287515	28.008221739489098	22.53382187147689
32	22.584348447768583	28.13814722138202	27.080870019523196	22.196634311326203
33	23.292407952264416	27.3462204746061	26.03184205460995	23.329529518519536
34	21.18885253114087	25.41314928369126	29.342879533643142	24.055118651524733
35	21.06614513157533	27.468240437759505	27.4541479913108	24.011466439354358
36	22.25437896994528	28.61557180960761	28.700470206505894	20.42957901394121
37	20.005430747655844	29.355940825473642	28.52757994885473	22.111048478015782
38	22.5276349437677	26.77186735226991	27.172986498748863	23.527511205213518
39	22.6871201913823	25.640690736106908	26.592446448703495	25.0797426238073
40	25.05602606758875	24.654219484697666	27.841174691341052	22.448579756372535
41	20.799419803668158	27.054747435862183	26.803145709021912	25.342687051447744
42	22.20179008441719	26.752275414524156	28.7468721643248	22.29906233673385
43	21.27787554651195	27.24344873099238	28.58635576209201	22.89231996040366
44	21.110141061951772	25.79330161960019	27.909230896142105	25.18732642230594
45	20.675337531278355	24.785519839414853	27.49848763989331	27.040654989413483
46	23.07586548244287	26.66119008991668	26.841298429895232	23.42164599774521
47	19.895097203508673	25.23579068936123	30.86383259548492	24.00527951164517
48	20.660213930211455	25.24129018065829	28.498707619545193	25.599788269585062
49	21.45454670442984	23.752646630186707	30.864176313690983	23.92863035169247
50	20.64027827425963	23.862980174333874	30.380908515962275	25.115833035444226
51	20.87400665438447	23.610691011081474	27.472365056232302	28.042937278301753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	161.0
2	236.0
3	1295.0
4	2354.0
5	1635.0
6	916.0
7	922.5
8	929.0
9	980.5
10	1032.0
11	1081.0
12	1130.0
13	1154.0
14	1178.0
15	1151.0
16	1124.0
17	1103.0
18	1082.0
19	1101.0
20	1120.0
21	1137.0
22	1154.0
23	1190.5
24	1227.0
25	1392.5
26	1639.0
27	1720.0
28	2357.5
29	2995.0
30	3426.0
31	3857.0
32	4724.5
33	5592.0
34	6637.0
35	7682.0
36	8518.0
37	9354.0
38	10431.5
39	11509.0
40	12637.5
41	13766.0
42	15574.5
43	17383.0
44	19703.5
45	22024.0
46	29052.5
47	36081.0
48	35486.5
49	34892.0
50	32409.5
51	29927.0
52	24946.0
53	19965.0
54	17424.0
55	14883.0
56	13224.5
57	11566.0
58	10447.0
59	9328.0
60	8858.5
61	8389.0
62	6885.5
63	5382.0
64	4550.0
65	3718.0
66	2925.0
67	2132.0
68	1723.0
69	1314.0
70	1153.5
71	993.0
72	812.5
73	632.0
74	502.0
75	304.5
76	237.0
77	140.0
78	43.0
79	32.5
80	22.0
81	24.5
82	27.0
83	22.5
84	18.0
85	10.5
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	290936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.205247579681235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82538962505897	36.26561537656383
2	8.871396497661367	8.375529360992436
3	3.7449120490900203	5.3033850132427744
4	2.258166590839274	4.263892519869307
5	1.476920675037566	3.485920306034912
6	1.1819369685569698	3.3476176334585808
7	0.9122322478728733	3.0143504377705685
8	0.7295481156177005	2.755079953521877
9	0.5981638548904269	2.5412825576987212
>10	3.3444380296965663	24.56408298019425
>50	0.03793023045219574	1.1652061597582493
>100	0.012400267647833224	1.3542422654637098
>500	0.0036471375434803596	1.2023935903888316
>1k	0.002917710034784288	2.361401845041984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	1877	0.6451590727857673	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	1736	0.59669480573047	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	1688	0.5801963318393049	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1557	0.5351692468446668	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	981	0.33718756015068607	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	717	0.24644595374927822	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	709	0.24369620810075066	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	567	0.19488822283938737	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	518	0.17804603074215636	No Hit
GAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	396	0.13611240960211182	No Hit
TCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	340	0.11686419006241922	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCT	338	0.11617675365028736	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTC	301	0.10345918002584761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.437182060659389E-4	0.0	0.0	0.2103555421123546	0.0
2	3.437182060659389E-4	0.0	0.0	1.0617455385376853	0.0
3	6.874364121318778E-4	0.0	0.0	1.5965710671762863	0.0
4	6.874364121318778E-4	0.0	0.0	2.276445678774713	0.0
5	6.874364121318778E-4	0.0	0.0	4.273448456017818	0.0
6	6.874364121318778E-4	0.0	0.0	5.618074078147771	0.0
7	6.874364121318778E-4	0.0	0.0	6.7671240410262055	0.0
8	6.874364121318778E-4	0.0	0.0	8.527648692495944	0.0
9	6.874364121318778E-4	0.0	0.0	9.278329254543955	0.0
10	6.874364121318778E-4	0.0	0.0	11.247147138889654	0.0
11	6.874364121318778E-4	0.0	0.0	12.960926114334423	0.0
12	6.874364121318778E-4	0.0	0.0	14.969959028789837	0.0
13	6.874364121318778E-4	0.0	0.0	15.725795363928837	0.0
14	6.874364121318778E-4	0.0	0.0	16.072950752055434	0.0
15	6.874364121318778E-4	0.0	0.0	16.52219044738362	0.0
16	6.874364121318778E-4	0.0	0.0	17.24159265267963	0.0
17	6.874364121318778E-4	0.0	0.0	18.20022272939753	0.0
18	6.874364121318778E-4	0.0	0.0	19.249250694310778	0.0
19	6.874364121318778E-4	0.0	0.0	19.95043583468529	0.0
20	6.874364121318778E-4	0.0	0.0	20.5760029697253	0.0
21	6.874364121318778E-4	0.0	0.0	21.32359006791872	0.0
22	6.874364121318778E-4	0.0	0.0	22.135452470646467	0.0
23	6.874364121318778E-4	0.0	0.0	22.89850688811285	0.0
24	6.874364121318778E-4	0.0	0.0	23.52132427750433	0.0
25	6.874364121318778E-4	0.0	0.0	24.06577391591278	0.0
26	6.874364121318778E-4	0.0	0.0	24.59544367146039	0.0
27	6.874364121318778E-4	0.0	0.0	25.185951549481672	0.0
28	6.874364121318778E-4	0.0	0.0	25.709778095526165	0.0
29	6.874364121318778E-4	0.0	0.0	26.284474936068413	0.0
30	6.874364121318778E-4	0.0	0.0	26.966411856903235	0.0
31	6.874364121318778E-4	0.0	0.0	27.569981026755027	0.0
32	6.874364121318778E-4	0.0	0.0	28.107556301042152	0.0
33	6.874364121318778E-4	0.0	0.0	28.69875161547557	0.0
34	6.874364121318778E-4	0.0	0.0	29.307820276624412	0.0
35	6.874364121318778E-4	0.0	0.0	29.927887920367365	0.0
36	6.874364121318778E-4	0.0	0.0	30.464432040036296	0.0
37	6.874364121318778E-4	0.0	0.0	31.013693733329667	0.0
38	6.874364121318778E-4	0.0	0.0	31.575329282041412	0.0
39	6.874364121318778E-4	0.0	0.0	32.255547611845905	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGG	30	2.1598935E-6	45.000004	2
AGCGTCG	20	7.0245925E-4	45.0	17
TTGGACG	20	7.0245925E-4	45.0	1
CATGCGG	40	6.7848305E-9	45.0	2
CCGATAC	20	7.0245925E-4	45.0	11
TTGCTAG	25	3.8835995E-5	45.0	1
TACGGGT	35	1.2077544E-7	45.0	4
CGCGAGG	20	7.0245925E-4	45.0	2
TCGATCA	20	7.0245925E-4	45.0	17
CCTCGAT	20	7.0245925E-4	45.0	15
CCCACGA	20	7.0245925E-4	45.0	39
GTTACGG	25	3.8835995E-5	45.0	2
TGCGCGC	20	7.0245925E-4	45.0	11
TACGATG	20	7.0245925E-4	45.0	1
TGATTAG	25	3.8835995E-5	45.0	1
GATGCGC	20	7.0245925E-4	45.0	9
CTCGATC	20	7.0245925E-4	45.0	16
ATGCGAG	25	3.8835995E-5	45.0	1
ACGTACC	20	7.0245925E-4	45.0	35
CCAACGG	20	7.0245925E-4	45.0	2
>>END_MODULE