##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683263
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848185837664268	33.0	31.0	34.0	30.0	34.0
2	31.901654267829517	33.0	31.0	34.0	30.0	34.0
3	31.973458243750944	33.0	31.0	34.0	30.0	34.0
4	35.713779320700816	37.0	35.0	37.0	33.0	37.0
5	35.73444486237364	37.0	35.0	37.0	35.0	37.0
6	35.68467047096067	37.0	35.0	37.0	33.0	37.0
7	35.97021937379896	37.0	35.0	37.0	35.0	37.0
8	35.834017062243966	37.0	35.0	37.0	35.0	37.0
9	37.63937605285227	39.0	37.0	39.0	35.0	39.0
10	36.967770829095095	39.0	37.0	39.0	32.0	39.0
11	36.94192572991659	39.0	37.0	39.0	33.0	39.0
12	36.9845052344412	39.0	37.0	39.0	33.0	39.0
13	36.96784547092408	39.0	37.0	39.0	33.0	39.0
14	37.95135109028295	40.0	37.0	41.0	33.0	41.0
15	38.09695241802937	40.0	37.0	41.0	33.0	41.0
16	38.03348783704079	40.0	37.0	41.0	33.0	41.0
17	38.055368137891264	40.0	37.0	41.0	33.0	41.0
18	37.952621464941025	39.0	37.0	41.0	33.0	41.0
19	37.815138533771034	39.0	37.0	41.0	33.0	41.0
20	37.84634906324504	39.0	36.0	41.0	33.0	41.0
21	37.760011884150025	39.0	36.0	41.0	33.0	41.0
22	37.86586131548174	39.0	36.0	41.0	33.0	41.0
23	37.85327758125349	39.0	36.0	41.0	34.0	41.0
24	37.83413122033536	40.0	36.0	41.0	33.0	41.0
25	37.57375856734522	39.0	35.0	41.0	33.0	41.0
26	37.671652057845954	39.0	36.0	41.0	33.0	41.0
27	37.67957140954508	39.0	36.0	41.0	33.0	41.0
28	37.55774569967933	39.0	35.0	41.0	33.0	41.0
29	37.52092093381319	39.0	35.0	41.0	33.0	41.0
30	37.3878535790757	39.0	35.0	41.0	33.0	41.0
31	37.35071707380613	39.0	35.0	41.0	33.0	41.0
32	37.2956372582739	40.0	35.0	41.0	33.0	41.0
33	37.13478265323894	40.0	35.0	41.0	32.0	41.0
34	37.013413575738774	40.0	35.0	41.0	31.0	41.0
35	36.915233226444286	40.0	35.0	41.0	31.0	41.0
36	36.82067666476891	40.0	35.0	41.0	31.0	41.0
37	36.752032526274654	40.0	35.0	41.0	31.0	41.0
38	36.664638653051604	39.0	35.0	41.0	31.0	41.0
39	36.5827960829139	39.0	35.0	41.0	31.0	41.0
40	36.500308080490235	39.0	35.0	41.0	30.0	41.0
41	36.36890333590433	39.0	35.0	41.0	30.0	41.0
42	36.38760038228325	39.0	35.0	41.0	30.0	41.0
43	36.33722300197728	39.0	35.0	41.0	30.0	41.0
44	36.2127701924442	39.0	35.0	41.0	30.0	41.0
45	36.188234984186174	39.0	35.0	41.0	30.0	41.0
46	36.080561950522714	39.0	35.0	41.0	30.0	41.0
47	36.04962364418972	39.0	35.0	41.0	30.0	41.0
48	35.95438652466181	39.0	35.0	41.0	29.0	41.0
49	35.8822517829884	39.0	35.0	40.0	29.0	41.0
50	35.75119243980722	38.0	35.0	40.0	29.0	41.0
51	34.55032981443456	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	8.0
11	11.0
12	7.0
13	16.0
14	18.0
15	29.0
16	47.0
17	91.0
18	173.0
19	357.0
20	600.0
21	930.0
22	1357.0
23	2132.0
24	3143.0
25	5120.0
26	7337.0
27	8941.0
28	9645.0
29	9664.0
30	11102.0
31	13381.0
32	17651.0
33	24479.0
34	41319.0
35	48208.0
36	53599.0
37	79184.0
38	142397.0
39	202231.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.28716028820527	21.39747066649299	25.419933466322632	14.895435578979106
2	33.56994305267518	24.20868684532896	25.461352363584737	16.760017738411122
3	29.767015043987456	23.504419235345686	30.363271536728902	16.365294183937955
4	27.104497097018275	25.382758908355935	29.63178161264403	17.880962381981753
5	24.663270219520157	28.602456155243296	28.383799503265944	18.350474121970603
6	24.940908552050967	33.53950089497017	26.97394707455255	14.545643478426316
7	78.08018288711668	5.853090244898378	11.346436145378865	4.720290722606083
8	80.17966727307055	4.826106491936486	10.344333002079727	4.649893232913241
9	73.82442778256689	7.2374766378393085	12.807074289109757	6.131021290484044
10	38.4920594266044	27.92189830270335	19.22890599959313	14.357136271099122
11	27.7775907666594	24.90783197685225	28.79725669324989	18.51732056323846
12	25.69552280746945	21.466843660493836	32.36206263181235	20.475570900224366
13	24.372752512575683	21.91103572123765	33.84714231562371	19.86906945056296
14	20.122998025650446	25.55399604544663	33.47466495331958	20.84834097558334
15	19.330623786155552	24.956568700485757	36.0247810872241	19.68802642613459
16	22.922505682292176	24.44490628059766	32.555838674126946	20.076749362983215
17	23.19311890150645	24.562430572122302	30.62115173805694	21.62329878831431
18	23.59969733470128	24.274400926144104	31.981828373554542	20.144073365600068
19	23.794497872707872	26.48526262947064	29.29238082553863	20.427858672282852
20	23.969101209929413	26.416767774634366	29.483522450359523	20.1306085650767
21	23.791570742159315	26.34051602384441	30.758434160784354	19.109479073211926
22	22.57886055589136	24.253032873139627	30.7634102827169	22.404696288252108
23	21.17456967522023	25.772945410478837	30.908010531815716	22.14447438248522
24	21.57324485593395	24.795722876842447	31.41967295170381	22.211359315519793
25	22.290245483803456	26.302756039767992	28.96966468256001	22.43733379386854
26	21.019578112674036	26.379885929722523	29.54089420911128	23.05964174849216
27	21.108123811767943	25.486232973247493	30.555144944186942	22.85049827079763
28	20.34619172997806	25.730209304469874	30.67003481821787	23.25356414733419
29	21.372150987247956	24.911344533510523	30.156030693890933	23.560473785350588
30	22.308100980149664	24.79308845934874	30.302826290901162	22.595984269600432
31	21.52421541924559	25.377636429895954	29.768039539679446	23.330108611179003
32	22.49865717886085	26.346516641468952	27.69899731143059	23.45582886823961
33	21.615980961942913	25.41846990104835	29.032158919771746	23.933390217236994
34	20.08626253726603	25.382027125718793	29.63075711695204	24.900953220063137
35	19.506397975596514	27.252902615830216	29.472984780384714	23.76771462818856
36	20.579044965115923	26.919795159404213	28.059034368903333	24.44212550657653
37	20.31926212893132	27.771443792507423	29.077236730219546	22.83205734834171
38	20.12124174732131	27.896578623458318	27.881503901133236	24.100675728087133
39	21.02352973891459	25.52209032246734	28.36257780678889	25.091802131829176
40	21.949088418368916	25.116829098019355	29.768624965789165	23.165457517822567
41	19.923514078766154	25.26040485142617	29.222568761955497	25.593512307852173
42	20.45317835152789	26.01823895044807	29.209835744069267	24.318746953954772
43	20.764917754949412	25.20903371029896	29.89654057076119	24.12950796399044
44	21.02206617364031	25.184592170218494	28.846871556047965	24.94647010009323
45	20.716034674788478	24.91851600335449	28.996447927079323	25.369001394777708
46	21.183497423393334	25.4234460229809	28.553719431609792	24.839337122015973
47	19.766327168308543	25.110828480394808	30.714234489501113	24.40860986179553
48	19.97883684613392	24.99754852816558	29.96488906907004	25.058725556630463
49	21.09758614179313	24.321235014921047	29.906492814626283	24.674686028659533
50	19.82121086609402	24.214102036843794	30.708233871876566	25.256453225185616
51	19.71305339232477	24.17370763527368	29.345361888467547	26.767877083934007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	454.0
2	624.0
3	5084.0
4	9544.0
5	6296.5
6	3049.0
7	2968.0
8	2887.0
9	2916.5
10	2946.0
11	2932.0
12	2918.0
13	2833.5
14	2749.0
15	2651.0
16	2553.0
17	2539.5
18	2526.0
19	2557.5
20	2589.0
21	2500.0
22	2411.0
23	2667.0
24	2923.0
25	3703.5
26	5015.0
27	5546.0
28	6734.5
29	7923.0
30	9487.0
31	11051.0
32	12470.5
33	13890.0
34	16268.5
35	18647.0
36	20727.0
37	22807.0
38	24034.0
39	25261.0
40	27701.0
41	30141.0
42	32969.5
43	35798.0
44	40017.5
45	44237.0
46	53062.5
47	61888.0
48	64302.5
49	66717.0
50	64374.0
51	62031.0
52	55281.0
53	48531.0
54	43389.5
55	38248.0
56	35782.0
57	33316.0
58	30979.5
59	28643.0
60	26591.0
61	24539.0
62	21991.0
63	19443.0
64	16976.5
65	14510.0
66	11970.0
67	9430.0
68	8222.0
69	7014.0
70	5475.0
71	3936.0
72	3435.0
73	2934.0
74	2402.5
75	1508.5
76	1146.0
77	876.5
78	607.0
79	493.0
80	379.0
81	251.0
82	123.0
83	120.5
84	118.0
85	73.5
86	29.0
87	21.0
88	13.0
89	9.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	683263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.88887294133174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18387063816012	26.996705053259472
2	8.619825913100545	6.359513257695297
3	3.480870371291029	3.852161545554031
4	2.0963217464716317	3.093237861989707
5	1.5617795541382191	2.8806143767487256
6	1.2846009025247158	2.8432487684132597
7	1.069061803865159	2.7605539534448935
8	0.9630900490144941	2.842184515932531
9	0.8602267478799223	2.855951568294972
>10	6.826206706931866	41.23969722687663
>50	0.0346372375766576	0.9010189986243564
>100	0.01473077907531392	0.9933973053433697
>500	0.001990645820988367	0.5552979319194679
>1k	0.00238877498518604	0.9819418071445547
>5k	3.981291641976733E-4	0.844475828758778
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5750	0.841550032710684	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	1239	0.18133573748322387	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1216	0.17796953735238114	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1144	0.1674318673775691	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1055	0.154406136436482	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1017	0.14884458839422007	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1015	0.1485518753393642	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	843	0.12337855262175765	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	782	0.11445080444865302	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	750	0.10976739557095876	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	746	0.10918196946124699	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.854261097117801E-4	0.0	0.0	0.15952861489646009	0.0
2	5.854261097117801E-4	0.0	0.0	0.6407488770795433	0.0
3	5.854261097117801E-4	0.0	0.0	1.0231784832487636	0.0
4	5.854261097117801E-4	0.0	0.0	1.482737979372511	0.0
5	5.854261097117801E-4	0.0	0.0	2.568264343305579	0.0
6	5.854261097117801E-4	0.0	0.0	3.6562787682049227	0.0
7	5.854261097117801E-4	0.0	0.0	4.56237202951133	0.0
8	5.854261097117801E-4	0.0	0.0	6.0208148253308025	0.0
9	5.854261097117801E-4	0.0	0.0	6.772794663255584	0.0
10	5.854261097117801E-4	0.0	0.0	8.033363433992474	0.0
11	5.854261097117801E-4	0.0	0.0	9.219729445323397	0.0
12	5.854261097117801E-4	0.0	0.0	10.227101423609943	0.0
13	5.854261097117801E-4	0.0	0.0	10.713444164253003	0.0
14	5.854261097117801E-4	0.0	0.0	10.959762199914236	0.0
15	5.854261097117801E-4	0.0	0.0	11.24354750659702	0.0
16	5.854261097117801E-4	0.0	0.0	11.716571803244138	0.0
17	5.854261097117801E-4	0.0	0.0	12.29263109520053	0.0
18	5.854261097117801E-4	0.0	0.0	12.94216136392575	0.0
19	5.854261097117801E-4	0.0	0.0	13.338787553255482	0.0
20	5.854261097117801E-4	0.0	0.0	13.746829551724591	0.0
21	5.854261097117801E-4	0.0	0.0	14.22409818766712	0.0
22	5.854261097117801E-4	0.0	0.0	14.75478695612085	0.0
23	5.854261097117801E-4	0.0	0.0	15.281231385279169	0.0
24	5.854261097117801E-4	0.0	0.0	15.690005166385419	0.0
25	5.854261097117801E-4	0.0	0.0	16.04960315427588	0.0
26	5.854261097117801E-4	0.0	0.0	16.401883315794944	0.0
27	5.854261097117801E-4	0.0	0.0	16.793387026664696	0.0
28	5.854261097117801E-4	0.0	0.0	17.165864388968817	0.0
29	7.317826371397251E-4	0.0	0.0	17.5774189440962	0.0
30	8.781391645676701E-4	0.0	0.0	18.071225867638084	0.0
31	0.0010244956919956152	0.0	0.0	18.482048640128326	0.0
32	0.0010244956919956152	0.0	0.0	18.88321188180832	0.0
33	0.0010244956919956152	0.0	0.0	19.298278993593975	0.0
34	0.0010244956919956152	0.0	0.0	19.72871354075956	0.0
35	0.0010244956919956152	0.0	0.0	20.13982902630466	0.0
36	0.0010244956919956152	0.0	0.0	20.518014293178467	0.0
37	0.0010244956919956152	0.0	0.0	20.923714587208732	0.0
38	0.0010244956919956152	0.0	0.0	21.377858891817645	0.0
39	0.0010244956919956152	0.0	0.0	21.951283766280334	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGG	20	7.0312765E-4	45.0	2
CGATCAC	20	7.0312765E-4	45.0	10
CGTTTTT	2920	0.0	42.6113	1
CGACAGG	70	0.0	41.785713	2
CACGACG	45	1.9263098E-8	40.0	26
CGAATAT	40	3.4554796E-7	39.375	14
CGAGATA	110	0.0	38.863636	19
AACCCGC	35	6.2445706E-6	38.571426	21
AGGGTAC	175	0.0	38.571426	6
TATTGCG	35	6.2445706E-6	38.571426	1
CCGTAGG	35	6.2445706E-6	38.571426	1
GCGATAT	35	6.2445706E-6	38.571426	9
GGCGATA	210	0.0	38.571426	8
TACGGTT	65	9.094947E-12	38.07692	33
AGGGCGA	720	0.0	37.8125	6
GCGATCG	30	1.1394374E-4	37.499996	9
TATGGGA	460	0.0	37.173912	4
GGGCGAT	1295	0.0	36.833977	7
CCACGTC	55	2.746674E-9	36.81818	33
GCGATGT	160	0.0	36.5625	9
>>END_MODULE