##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572742
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745997325148146	31.0	31.0	34.0	30.0	34.0
2	31.799291827733953	33.0	31.0	34.0	30.0	34.0
3	31.873824514353757	33.0	31.0	34.0	30.0	34.0
4	35.6242304562962	37.0	35.0	37.0	33.0	37.0
5	35.64277109064815	37.0	35.0	37.0	33.0	37.0
6	35.59465867703084	37.0	35.0	37.0	33.0	37.0
7	35.88859381711137	37.0	35.0	37.0	35.0	37.0
8	35.7020543281268	37.0	35.0	37.0	35.0	37.0
9	37.52292480733035	39.0	37.0	39.0	35.0	39.0
10	36.88240254774401	39.0	37.0	39.0	32.0	39.0
11	36.84650680411075	39.0	37.0	39.0	32.0	39.0
12	36.957977588512804	39.0	37.0	39.0	33.0	39.0
13	36.92196486376065	39.0	37.0	39.0	33.0	39.0
14	37.99766386959573	40.0	37.0	41.0	33.0	41.0
15	38.07145101983092	40.0	37.0	41.0	33.0	41.0
16	38.043337139584665	40.0	37.0	41.0	33.0	41.0
17	38.019373470079024	40.0	37.0	41.0	33.0	41.0
18	37.89883053800839	39.0	37.0	41.0	33.0	41.0
19	37.70157418174326	39.0	37.0	41.0	33.0	41.0
20	37.66638032482339	39.0	36.0	41.0	33.0	41.0
21	37.65622566530829	39.0	36.0	41.0	33.0	41.0
22	37.756033257557505	39.0	36.0	41.0	33.0	41.0
23	37.754201717352665	39.0	36.0	41.0	33.0	41.0
24	37.736357731753564	39.0	36.0	41.0	33.0	41.0
25	37.48309535532579	39.0	35.0	41.0	33.0	41.0
26	37.53595685317298	39.0	35.0	41.0	33.0	41.0
27	37.568414748700114	39.0	35.0	41.0	33.0	41.0
28	37.43242856294806	39.0	35.0	41.0	33.0	41.0
29	37.36980699861368	39.0	35.0	41.0	33.0	41.0
30	37.2290490308027	39.0	35.0	41.0	32.0	41.0
31	37.19368232118476	39.0	35.0	41.0	32.0	41.0
32	37.07949652723216	39.0	35.0	41.0	32.0	41.0
33	36.84190263678934	39.0	35.0	41.0	31.0	41.0
34	36.67850096553073	39.0	35.0	41.0	31.0	41.0
35	36.56318726407353	39.0	35.0	41.0	30.0	41.0
36	36.43285982169982	39.0	35.0	41.0	30.0	41.0
37	36.37014746604929	39.0	35.0	41.0	30.0	41.0
38	36.25553041334493	39.0	35.0	41.0	30.0	41.0
39	36.14420454585136	39.0	35.0	41.0	29.0	41.0
40	36.007333144766754	39.0	35.0	41.0	28.0	41.0
41	35.87578351159859	39.0	35.0	41.0	27.0	41.0
42	35.88778717118703	39.0	35.0	41.0	27.0	41.0
43	35.809025704418396	39.0	35.0	41.0	27.0	41.0
44	35.60302544601234	39.0	35.0	41.0	26.0	41.0
45	35.60292068680139	39.0	35.0	41.0	26.0	41.0
46	35.42863278753784	39.0	35.0	40.0	25.0	41.0
47	35.35924552416271	38.0	35.0	40.0	25.0	41.0
48	35.28327763635284	38.0	35.0	40.0	24.0	41.0
49	35.20625691847289	38.0	35.0	40.0	24.0	41.0
50	35.08999514615656	38.0	35.0	40.0	24.0	41.0
51	33.83712212479615	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	7.0
12	6.0
13	5.0
14	7.0
15	17.0
16	27.0
17	75.0
18	169.0
19	339.0
20	643.0
21	1047.0
22	1634.0
23	2435.0
24	3465.0
25	5330.0
26	7935.0
27	9163.0
28	9201.0
29	9334.0
30	10351.0
31	12467.0
32	15527.0
33	21239.0
34	33084.0
35	41915.0
36	45462.0
37	65238.0
38	117158.0
39	159399.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54153877312996	20.698499498901775	24.58943119240426	15.170530535564005
2	32.748253140157345	27.190776999067644	24.561320804131704	15.499649056643305
3	28.023787324833872	26.489414081733138	30.41910668328846	15.067691910144532
4	24.938977759619515	24.503528639422285	33.899382269852744	16.658111331105456
5	25.523184959370887	28.362508773583915	28.88927300599572	17.22503326104948
6	23.160864752366685	34.81550156964218	28.030072877491087	13.993560800500052
7	74.14001417741322	5.58680872015672	15.732912899700038	4.540264202730025
8	75.99372841523757	6.99966127855125	12.238145622287174	4.768464683924001
9	69.74815885686749	7.306431167960444	14.22665004487186	8.718759930300205
10	36.11172220650834	30.067464931854133	20.394872385821188	13.425940475816336
11	25.892635776667326	24.3921346784416	31.731390399167513	17.983839145723554
12	23.605742201549738	20.951667592039698	34.672679845375406	20.76991036103516
13	22.597958592175885	22.83960317210891	36.99117578246401	17.5712624532512
14	19.145094999144465	27.513609967489728	33.126783089069775	20.214511944296035
15	17.410107867067545	25.250985609576386	38.66435498007829	18.674551543277776
16	19.867758956039545	26.07596439583617	33.118402352193485	20.937874295930804
17	20.275097687964212	26.14754985665448	31.890973597186868	21.68637885819444
18	20.745641143830902	25.345618096804497	33.143020766767584	20.765719992597013
19	21.003872598831585	27.747223007916304	30.642243802619678	20.60666059063243
20	21.685855062139673	26.766327596020545	31.817292952149483	19.730524389690295
21	21.303309343474027	28.912145433720593	31.382891424061793	18.401653798743588
22	19.88259984425797	25.077259918078298	31.85395867598325	23.186181561680478
23	18.907291590279744	28.31763691155878	31.266957897273116	21.50811360088836
24	20.24332072730828	25.94641217162352	31.453080095400722	22.357187005667473
25	19.028986873670867	30.194572774477862	29.47592458733601	21.300515764515264
26	18.146390521386593	28.255828977096144	31.141945238868463	22.4558352626488
27	20.474663984830865	28.01575578532742	30.059084194977842	21.450496034863864
28	18.727280346124434	27.40815236179641	33.04943587164901	20.81513142043014
29	20.34127058954992	26.38570246288905	31.416239772882033	21.856787174679003
30	20.485663701980997	28.887177821776643	31.610917306570848	19.01624116967151
31	21.94129293818159	28.581106327107143	28.355874023556854	21.121726711154412
32	23.09277126524683	30.247650774694364	27.17017435424676	19.489403605812043
33	21.53901756811968	28.507949478124534	28.133260700280406	21.819772253475385
34	21.813137503448324	29.1513456320647	27.84709345569209	21.188423408794886
35	21.09379092156678	27.45407181593108	28.236448523069726	23.215688739432412
36	20.52913877452675	30.630371092044932	27.13839739359083	21.702092739837482
37	21.86324732602114	28.190703667619978	29.509971330895933	20.43607767546295
38	20.37165076072647	29.747250943705893	26.706091049722215	23.175007245845425
39	23.978859591229558	27.32539258514305	26.67309189827182	22.02265592535557
40	22.105066504639087	26.642013332355578	28.6891829130743	22.563737249931034
41	20.424204964888204	26.88278491886399	27.50313404639436	25.18987606985344
42	20.240177950979675	26.77715271448575	29.190455737487387	23.792213597047187
43	21.015570710721406	26.23205562015707	28.798656288520835	23.95371738060069
44	21.159440027097716	26.824468958099807	28.670675452472494	23.345415562329986
45	20.22690845092555	26.418352416969594	28.323049470791382	25.03168966131347
46	22.852523474793188	27.565291178226847	27.29763139424034	22.28455395273963
47	19.49708594794864	25.83833558565637	31.569712016929092	23.094866449465904
48	20.772354742624078	25.60646853207902	29.011666684126535	24.60951004117037
49	20.357682865932652	24.483449790656174	31.42723949003216	23.73162785337901
50	20.245415911527353	24.51836952764072	31.073677153063684	24.162537407768244
51	19.85850522573864	24.340802665074328	28.662294715596204	27.13839739359083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	375.5
2	505.0
3	5143.0
4	9781.0
5	6470.0
6	3159.0
7	3100.5
8	3042.0
9	3104.5
10	3167.0
11	3251.5
12	3336.0
13	3319.5
14	3303.0
15	3269.0
16	3235.0
17	3082.5
18	2930.0
19	2931.5
20	2933.0
21	2898.5
22	2864.0
23	2924.0
24	2984.0
25	3510.5
26	4805.0
27	5573.0
28	6333.5
29	7094.0
30	8501.5
31	9909.0
32	11142.5
33	12376.0
34	14478.5
35	16581.0
36	18108.0
37	19635.0
38	21088.0
39	22541.0
40	24752.5
41	26964.0
42	29509.0
43	32054.0
44	36565.0
45	41076.0
46	56311.5
47	71547.0
48	65884.0
49	60221.0
50	56406.0
51	52591.0
52	44534.5
53	36478.0
54	31746.0
55	27014.0
56	24290.0
57	21566.0
58	19911.5
59	18257.0
60	16682.0
61	15107.0
62	13347.5
63	11588.0
64	9402.5
65	7217.0
66	5733.0
67	4249.0
68	3524.0
69	2799.0
70	2309.0
71	1819.0
72	1590.0
73	1361.0
74	1036.0
75	583.5
76	456.0
77	366.5
78	277.0
79	170.0
80	63.0
81	51.0
82	39.0
83	30.0
84	21.0
85	14.5
86	8.0
87	16.0
88	24.0
89	14.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.65887049214911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83185676200128	30.83237643265412
2	8.69195263333954	7.068099528856936
3	3.4759361352957034	4.239829114919079
4	2.0784270009694636	3.380259770392132
5	1.4379431517000534	2.9232572190022603
6	1.13726290550289	2.7743895114220334
7	0.939051154267483	2.6726531486805305
8	0.7527149028759506	2.44836302028351
9	0.6569762026499688	2.4040719305972096
>10	4.931713704941527	33.17086541625634
>50	0.04511280440608417	1.2270163198594035
>100	0.013319018439835162	0.9929329100920117
>500	0.004296457561237149	1.286585306619927
>1k	0.002577874536742289	2.5455697336137924
>5k	8.592915122474296E-4	2.033730636750739
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6450	1.1261615177514483	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	5192	0.9065163721186853	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	4694	0.819566227027178	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	4503	0.7862178782069413	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1786	0.3118332512719514	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	1388	0.24234297467271476	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	1160	0.20253447451033796	No Hit
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	1041	0.18175723100453606	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	959	0.16744013884087425	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	865	0.15102786245813998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	860	0.15015486903352643	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	808	0.14107573741754575	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC	753	0.13147280974679698	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGAGCCT	707	0.12344127024035256	No Hit
CGTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	698	0.1218698820760482	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	591	0.10318782278931876	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTA	588	0.10266402673455063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.237960547681155E-4	0.0	0.0	0.18524920470299017	0.0
2	5.237960547681155E-4	0.0	0.0	0.8689776548603035	0.0
3	5.237960547681155E-4	0.0	0.0	1.339521110726994	0.0
4	5.237960547681155E-4	0.0	0.0	2.0703912058134377	0.0
5	5.237960547681155E-4	0.0	0.0	4.117036990477388	0.0
6	5.237960547681155E-4	0.0	0.0	5.459526278848068	0.0
7	5.237960547681155E-4	0.0	0.0	6.590576559777352	0.0
8	5.237960547681155E-4	0.0	0.0	8.276850658760837	0.0
9	5.237960547681155E-4	0.0	0.0	9.08122680019974	0.0
10	6.983947396908206E-4	0.0	0.0	11.158252756040241	0.0
11	6.983947396908206E-4	0.0	0.0	12.506154603643525	0.0
12	6.983947396908206E-4	0.0	0.0	14.52922956584291	0.0
13	6.983947396908206E-4	0.0	0.0	15.182752443508596	0.0
14	6.983947396908206E-4	0.0	0.0	15.509251984314053	0.0
15	6.983947396908206E-4	0.0	0.0	15.924273058375324	0.0
16	6.983947396908206E-4	0.0	0.0	16.483163448812903	0.0
17	6.983947396908206E-4	0.0	0.0	17.16968547792898	0.0
18	6.983947396908206E-4	0.0	0.0	18.00933055372227	0.0
19	6.983947396908206E-4	0.0	0.0	18.648012543169525	0.0
20	6.983947396908206E-4	0.0	0.0	19.171633999252716	0.0
21	6.983947396908206E-4	0.0	0.0	19.72301664623862	0.0
22	6.983947396908206E-4	0.0	0.0	20.33690562242685	0.0
23	6.983947396908206E-4	0.0	0.0	20.9570801512723	0.0
24	6.983947396908206E-4	0.0	0.0	21.45468640330201	0.0
25	6.983947396908206E-4	0.0	0.0	21.88507216163648	0.0
26	6.983947396908206E-4	0.0	0.0	22.317378505505097	0.0
27	6.983947396908206E-4	0.0	0.0	22.779890421865343	0.0
28	6.983947396908206E-4	0.0	0.0	23.203990627542595	0.0
29	6.983947396908206E-4	0.0	0.0	23.686232195299105	0.0
30	6.983947396908206E-4	0.0	0.0	24.270439395050477	0.0
31	0.001047592109536231	0.0	0.0	24.75529994308083	0.0
32	0.001047592109536231	0.0	0.0	25.234747931878577	0.0
33	0.001047592109536231	0.0	0.0	25.68678392714346	0.0
34	0.001047592109536231	0.0	0.0	26.186485363392244	0.0
35	0.001047592109536231	0.0	0.0	26.667504740354296	0.0
36	0.001222190794458936	0.0	0.0	27.108890215838894	0.0
37	0.001222190794458936	0.0	0.0	27.553069270282258	0.0
38	0.0013967894793816412	0.0	0.0	27.998994311574844	0.0
39	0.0013967894793816412	0.0	0.0	28.486124642509193	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	35	1.210501E-7	45.000004	9
TCGCCGA	20	7.03032E-4	45.0	14
AACGTTC	20	7.03032E-4	45.0	27
GCGTTAG	25	3.8883467E-5	45.0	1
CCATAGG	25	3.8883467E-5	45.0	2
CATACGG	25	3.8883467E-5	45.0	2
TCGACGT	20	7.03032E-4	45.0	26
CGATTCA	30	2.1635879E-6	44.999996	10
CGTTTTT	3435	0.0	43.689957	1
TGCAACG	60	3.6379788E-12	41.249996	1
TCACGAC	50	1.0786607E-9	40.5	25
TCGTAGG	50	1.0786607E-9	40.5	2
TTGTGCG	45	1.9254003E-8	40.0	1
CGGTCTA	45	1.9254003E-8	40.0	31
ACGGTCT	45	1.9254003E-8	40.0	30
CGCATGG	40	3.45417E-7	39.375	2
TATCGCG	40	3.45417E-7	39.375	1
ACAACGA	35	6.242799E-6	38.57143	13
TATTGCG	35	6.242799E-6	38.57143	1
CTACGAA	35	6.242799E-6	38.57143	11
>>END_MODULE