##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935396.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86803
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58283699872124	31.0	31.0	34.0	30.0	34.0
2	31.676739283204498	31.0	31.0	34.0	30.0	34.0
3	31.749559347027176	33.0	31.0	34.0	30.0	34.0
4	35.54448578966165	37.0	35.0	37.0	33.0	37.0
5	35.567906639171454	37.0	35.0	37.0	33.0	37.0
6	35.52629517412993	37.0	35.0	37.0	33.0	37.0
7	35.80308284275889	37.0	35.0	37.0	35.0	37.0
8	35.53961268619748	37.0	35.0	37.0	33.0	37.0
9	37.35403154268862	39.0	37.0	39.0	34.0	39.0
10	36.72223310254254	39.0	37.0	39.0	32.0	39.0
11	36.843404029814636	39.0	37.0	39.0	33.0	39.0
12	36.97274287755032	39.0	37.0	39.0	33.0	39.0
13	37.01971130030068	39.0	37.0	39.0	33.0	39.0
14	37.98542677096414	40.0	37.0	41.0	33.0	41.0
15	38.05230233978088	40.0	37.0	41.0	33.0	41.0
16	37.937755607525084	40.0	37.0	41.0	33.0	41.0
17	37.984217135352466	40.0	37.0	41.0	33.0	41.0
18	37.862481711461584	39.0	37.0	41.0	33.0	41.0
19	37.71194543967374	39.0	37.0	41.0	33.0	41.0
20	37.70490651244773	39.0	36.0	41.0	33.0	41.0
21	37.6626499084133	39.0	36.0	41.0	33.0	41.0
22	37.808232434362864	39.0	36.0	41.0	33.0	41.0
23	37.8291303295969	39.0	36.0	41.0	33.0	41.0
24	37.822863265094526	39.0	36.0	41.0	33.0	41.0
25	37.55223897791551	39.0	36.0	41.0	33.0	41.0
26	37.6503807472092	39.0	36.0	41.0	33.0	41.0
27	37.6742508899462	39.0	36.0	41.0	33.0	41.0
28	37.562111908574586	39.0	36.0	41.0	33.0	41.0
29	37.584254000437774	39.0	36.0	41.0	33.0	41.0
30	37.42078038777461	39.0	36.0	41.0	33.0	41.0
31	37.33118671013675	39.0	36.0	41.0	33.0	41.0
32	37.28251327719088	39.0	36.0	41.0	33.0	41.0
33	37.075711668951534	39.0	35.0	41.0	32.0	41.0
34	36.85638745204659	39.0	35.0	41.0	31.0	41.0
35	36.72177228897619	39.0	35.0	41.0	31.0	41.0
36	36.57064847989125	39.0	35.0	41.0	30.0	41.0
37	36.39864981625059	39.0	35.0	41.0	30.0	41.0
38	36.32256949644597	39.0	35.0	41.0	30.0	41.0
39	36.28979413153923	39.0	35.0	41.0	30.0	41.0
40	36.129016278239234	39.0	35.0	40.0	30.0	41.0
41	35.992385055816044	39.0	35.0	40.0	29.0	41.0
42	35.95679872815456	39.0	35.0	40.0	29.0	41.0
43	35.81542112599795	39.0	35.0	40.0	28.0	41.0
44	35.59555545315254	39.0	35.0	40.0	27.0	41.0
45	35.709376404041336	39.0	35.0	40.0	27.0	41.0
46	35.5727567019573	39.0	35.0	40.0	26.0	41.0
47	35.503565544969646	38.0	35.0	40.0	26.0	41.0
48	35.39449097381427	38.0	35.0	40.0	26.0	41.0
49	35.28447173484788	38.0	35.0	40.0	26.0	41.0
50	35.14316325472622	38.0	34.0	40.0	24.0	41.0
51	33.777127518634146	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	2.0
14	0.0
15	1.0
16	6.0
17	12.0
18	28.0
19	46.0
20	81.0
21	138.0
22	214.0
23	298.0
24	520.0
25	690.0
26	1038.0
27	1193.0
28	1305.0
29	1304.0
30	1570.0
31	1887.0
32	2472.0
33	3316.0
34	5042.0
35	6146.0
36	7436.0
37	11445.0
38	19560.0
39	21040.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.761194889577546	20.608734721150192	23.648952225153508	12.981118164118751
2	34.26609679388961	27.355045332534587	22.536087462414894	15.842770411160906
3	29.080792138520557	26.755987696277778	28.253631786919808	15.909588378281855
4	26.86888702003387	25.595889542988147	30.510466228125754	17.02475720885223
5	24.593620036173867	29.93214520235476	26.315910740412196	19.15832402105918
6	22.911650518991276	35.166987316106585	27.71102381254104	14.210338352361093
7	70.88119074225546	8.761217930255867	15.105468704998676	5.252122622490006
8	69.60128106171446	9.182862343467391	14.444201237284426	6.771655357533726
9	63.04505604645001	10.090665069179638	17.032821446263377	9.831457438106977
10	32.62099236201514	27.754801101344423	24.188104097784638	15.4361024388558
11	25.17424512977662	25.68114005276315	29.058903494118866	20.08571132334136
12	22.19623745723074	23.051046622812578	34.58521018858795	20.16750573136873
13	22.253839153024664	24.227273250924508	36.142760042855656	17.376127553195168
14	18.274714007580386	28.334274161031303	33.39515915348548	19.995852677902835
15	16.96945958089006	27.174176007741668	38.033247698812254	17.82311671255602
16	18.995887238920314	28.034745342902895	32.51039710609081	20.458970312085988
17	19.123763003582823	27.448360079720747	31.194774374157575	22.23310254253885
18	18.40143773832702	27.726000253447463	34.08407543518081	19.78848657304471
19	18.826538253286177	29.361888413994908	31.31919403707245	20.492379295646465
20	19.14219554623688	29.871087404813196	32.24542930543875	18.74128774351117
21	18.531617570821286	30.169464189025724	33.15553609898275	18.143382141170235
22	16.547815167678536	27.717936016036308	31.142932847943044	24.59131596834211
23	16.568551778164352	28.485190604011386	33.01037982558206	21.935877792242202
24	19.14334758015276	26.68340955957743	31.566881328986323	22.606361531283483
25	16.570855845996107	31.0380977615981	29.3676485835743	23.02339780883149
26	16.742508899462	29.500132483900327	31.53232031150997	22.225038305127704
27	20.334550649171113	27.65572618457887	30.996624540626478	21.013098625623538
28	15.676877527274403	29.21442807276246	31.42633319124915	23.682361208713985
29	18.167574853403686	28.781263320392153	29.988594864232805	23.06256696197136
30	21.667453889842516	30.023155881709158	28.39417992465698	19.915210303791344
31	19.606465214335913	32.95738626545165	25.836664631406748	21.599483888805686
32	18.64221282674562	32.498876766932014	26.143105653030425	22.715804753291938
33	21.122541847631997	30.32729283550108	25.802103613930395	22.748061702936536
34	20.07764708593021	30.713224197320372	26.99215464903287	22.216974067716553
35	20.538460652281604	31.539232515005246	24.81711461585429	23.105192216858864
36	23.219243574530836	33.20507355736553	25.1270117392256	18.448671128878033
37	21.689342534244208	30.986256235383568	26.987546513369352	20.33685471700287
38	18.781608930566914	32.682050159556695	25.088994620001614	23.447346289874773
39	23.826365448198793	27.381542112599792	26.50369226870039	22.288400170501017
40	22.472725597041578	29.062359595866504	28.4252848403857	20.039629966706222
41	22.321809154061494	30.08882181491423	26.040574634517238	21.548794396507034
42	22.27918389917399	29.166042648295566	28.594633826019837	19.960139626510603
43	22.984228655691624	26.773268205015953	28.74324620116816	21.499256938124258
44	20.871398453970485	27.083165328387267	26.425353962420655	25.620082255221593
45	21.22507286614518	25.33552987799961	26.788244645922376	26.651152609932836
46	23.246892388511917	28.492102807506654	26.87464718961326	21.38635761436817
47	20.482010990403555	27.289379399329516	30.866444708132207	21.36216490213472
48	19.907146066380193	27.040540073499763	29.213276038846587	23.83903782127346
49	21.50386507378777	24.473808508922502	31.81341658698432	22.208909830305405
50	20.380632005806252	23.60287086851837	30.21784961349262	25.79864751218276
51	19.553471654205502	23.388592560164973	27.971383477529578	29.08655230809995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	127.0
2	150.0
3	749.0
4	1348.0
5	916.5
6	485.0
7	474.0
8	463.0
9	468.5
10	474.0
11	488.5
12	503.0
13	494.5
14	486.0
15	509.5
16	533.0
17	510.5
18	488.0
19	502.0
20	516.0
21	555.0
22	594.0
23	552.0
24	510.0
25	665.0
26	911.5
27	1003.0
28	1109.0
29	1215.0
30	1408.0
31	1601.0
32	1671.0
33	1741.0
34	2044.5
35	2348.0
36	2524.5
37	2701.0
38	2958.5
39	3216.0
40	3608.0
41	4000.0
42	4508.0
43	5016.0
44	5534.5
45	6053.0
46	8262.5
47	10472.0
48	10709.0
49	10946.0
50	10619.5
51	10293.0
52	8292.5
53	6292.0
54	5112.0
55	3932.0
56	3202.0
57	2472.0
58	2142.5
59	1813.0
60	1633.5
61	1454.0
62	1294.0
63	1134.0
64	877.5
65	621.0
66	487.0
67	353.0
68	308.0
69	263.0
70	215.5
71	168.0
72	133.0
73	98.0
74	76.5
75	44.0
76	33.0
77	23.5
78	14.0
79	14.5
80	15.0
81	8.5
82	2.0
83	3.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	86803.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.70520604126586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32518831526731	50.36807483612318
2	10.124931104170495	12.697717820812645
3	3.8048870108396105	7.157586719353018
4	1.9878743340069813	4.986002787922077
5	1.1537754914569172	3.6173864958584376
6	0.7165166268601874	2.6957593631556516
7	0.48135219548043356	2.1128302017211387
8	0.3325372037479331	1.6681451101920441
9	0.22965276501929083	1.2960381553632938
>10	0.8010288443872865	7.96055435872032
>50	0.020209443321697593	0.9561881501791414
>100	0.01837222120154327	2.9250141124154694
>500	0.0036744442403086534	1.5587018881835881
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.9792288284967109	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	503	0.5794730596868772	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	427	0.4919184820801124	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	427	0.4919184820801124	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	373	0.4297086506226743	No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	278	0.32026542861421836	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	266	0.3064410216236766	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC	262	0.30183288596016267	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTT	156	0.1797172908770434	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	142	0.16358881605474465	No Hit
TCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	107	0.12326762899899774	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	101	0.11635542550372682	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	93	0.10713915417669896	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	92	0.10598712026082047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002304067831756967	0.0	0.0	0.3433061069317881	0.0
2	0.002304067831756967	0.0	0.0	1.467691208829188	0.0
3	0.002304067831756967	0.0	0.0	1.9215925716853104	0.0
4	0.002304067831756967	0.0	0.0	2.9019734340978998	0.0
5	0.002304067831756967	0.0	0.0	6.028593481792104	0.0
6	0.002304067831756967	0.0	0.0	7.7151711346382035	0.0
7	0.002304067831756967	0.0	0.0	9.352211329101529	0.0
8	0.002304067831756967	0.0	0.0	11.58370102415815	0.0
9	0.002304067831756967	0.0	0.0	12.467311037636948	0.0
10	0.002304067831756967	0.0	0.0	14.972984804672649	0.0
11	0.002304067831756967	0.0	0.0	18.01089824084421	0.0
12	0.002304067831756967	0.0	0.0	20.598366415907286	0.0
13	0.002304067831756967	0.0	0.0	21.601787956637445	0.0
14	0.002304067831756967	0.0	0.0	22.055689319493567	0.0
15	0.002304067831756967	0.0	0.0	22.80220729698282	0.0
16	0.002304067831756967	0.0	0.0	24.19501630127991	0.0
17	0.002304067831756967	0.0	0.0	25.757174291211133	0.0
18	0.002304067831756967	0.0	0.0	27.364261603861618	0.0
19	0.002304067831756967	0.0	0.0	28.48173450226375	0.0
20	0.002304067831756967	0.0	0.0	29.49667638215269	0.0
21	0.002304067831756967	0.0	0.0	30.66944690851699	0.0
22	0.002304067831756967	0.0	0.0	31.943596419478588	0.0
23	0.002304067831756967	0.0	0.0	33.063373385712474	0.0
24	0.002304067831756967	0.0	0.0	33.99191272191053	0.0
25	0.002304067831756967	0.0	0.0	34.83404951441771	0.0
26	0.002304067831756967	0.0	0.0	35.61973664504683	0.0
27	0.002304067831756967	0.0	0.0	36.3708627581996	0.0
28	0.002304067831756967	0.0	0.0	37.225671923781434	0.0
29	0.002304067831756967	0.0	0.0	38.07472091978388	0.0
30	0.002304067831756967	0.0	0.0	38.90072923746875	0.0
31	0.002304067831756967	0.0	0.0	39.73595382648065	0.0
32	0.002304067831756967	0.0	0.0	40.55620197458613	0.0
33	0.002304067831756967	0.0	0.0	41.30848012165478	0.0
34	0.002304067831756967	0.0	0.0	42.0250452173312	0.0
35	0.002304067831756967	0.0	0.0	42.70128912595187	0.0
36	0.002304067831756967	0.0	0.0	43.41324608596477	0.0
37	0.002304067831756967	0.0	0.0	44.080273723258415	0.0
38	0.002304067831756967	0.0	0.0	44.79568678501895	0.0
39	0.002304067831756967	0.0	0.0	45.489211202377795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGGCG	30	2.1423093E-6	45.000004	5
CGGGCGA	35	1.1946577E-7	45.000004	6
GCGGGCG	25	3.8609774E-5	45.0	5
AATCTAT	25	3.8609774E-5	45.0	16
GCCCAGG	20	6.9972646E-4	45.0	2
AGTAGGG	40	6.690243E-9	45.0	3
GCGATAT	20	6.9972646E-4	45.0	9
ATCTATA	25	3.8609774E-5	45.0	17
TCTGGGA	20	6.9972646E-4	45.0	4
TACGGGA	20	6.9972646E-4	45.0	4
AGCTACG	20	6.9972646E-4	45.0	9
GGATCTT	20	6.9972646E-4	45.0	8
GGCGATA	50	2.1827873E-11	45.0	8
TCTACGG	20	6.9972646E-4	45.0	2
CGAGGGT	20	6.9972646E-4	45.0	4
GCTACGA	20	6.9972646E-4	45.0	10
CGAATAT	20	6.9972646E-4	45.0	14
CGGGAAT	25	3.8609774E-5	45.0	6
ACGGGAC	20	6.9972646E-4	45.0	5
TACTGAC	20	6.9972646E-4	45.0	20
>>END_MODULE