FastQCFastQC Report
Sat 14 Jan 2017
SRR2935395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292349
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC147415.04226113309777No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG134714.607848838203654No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC131774.507284102220291TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37761.2916069492284905No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC35151.2023300917738728TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT30651.0484044754728081No Hit
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT27440.938604202511382TruSeq Adapter, Index 13 (100% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT25780.8818227529425448No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA23310.7973346924395158No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC22850.7816000738842959No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT12770.4368066933699106No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT9540.3263223065582574TruSeq Adapter, Index 13 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC7730.264410003112718TruSeq Adapter, Index 13 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG7410.25346418150908673TruSeq Adapter, Index 13 (100% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG6960.23807161987898026No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT5960.2038659273676325No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT5780.1977089027155899No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT5470.18710513803707213TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT4930.16863406408094433TruSeq Adapter, Index 13 (100% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT4920.16829200715583087No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACCAACTC4880.16692377945537695No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC4240.14503213624811442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.1286134038426675No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC3570.12211432226551143TruSeq Adapter, Index 13 (100% over 21bp)
GGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC3350.11458906991301493No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC2990.10227502060892973TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACCAACTCTCG2980.10193296368381626No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC2970.10159090675870279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA253.883645E-545.00000413
ACCGGCT253.883645E-545.00000434
AACCCTC302.159928E-645.00000439
ACGGCTA600.045.00000430
TCTAAAG253.883645E-545.00000424
CGGCTAT502.1827873E-1145.00000431
TGCTAGG302.159928E-645.0000042
CACAACG253.883645E-545.00000412
CACCGGT253.883645E-545.00000416
CGTTTTC253.883645E-545.0000041
ATCCTGC207.024648E-445.020
ACCGGTG207.024648E-445.017
CTCGTTC207.024648E-445.015
CGACGAA207.024648E-445.019
CTATCCC207.024648E-445.034
GTCCGCT207.024648E-445.026
CCGACTT351.2077726E-745.044
GGATTCA207.024648E-445.08
CCTATCA207.024648E-445.038
CGTTTTT20600.044.3446581
Produced by FastQC (version 0.11.5)