##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935395.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292349
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389219733948124	31.0	31.0	34.0	30.0	34.0
2	31.44141420015119	31.0	31.0	34.0	30.0	34.0
3	31.551724137931036	31.0	31.0	34.0	30.0	34.0
4	35.385022695476984	37.0	35.0	37.0	33.0	37.0
5	35.347365648591236	37.0	35.0	37.0	33.0	37.0
6	35.21942267632179	37.0	35.0	37.0	32.0	37.0
7	35.50029245867097	37.0	35.0	37.0	33.0	37.0
8	35.0203421253365	37.0	35.0	37.0	32.0	37.0
9	36.7979025069352	39.0	37.0	39.0	32.0	39.0
10	36.40153378325221	38.0	35.0	39.0	32.0	39.0
11	36.50794427208576	38.0	35.0	39.0	32.0	39.0
12	36.562430519687084	39.0	35.0	39.0	32.0	39.0
13	36.27199682571173	39.0	35.0	39.0	32.0	39.0
14	37.28825137079313	39.0	36.0	41.0	32.0	41.0
15	37.09534494730613	39.0	36.0	41.0	31.0	41.0
16	37.38652432537823	39.0	36.0	41.0	32.0	41.0
17	37.097838542290205	39.0	35.0	41.0	31.0	41.0
18	37.05153771690685	39.0	36.0	40.0	31.0	41.0
19	36.875894906430325	38.0	35.0	40.0	31.0	41.0
20	36.479888763087956	38.0	35.0	40.0	30.0	41.0
21	36.765988595822115	38.0	35.0	40.0	31.0	41.0
22	36.93658948722246	39.0	35.0	40.0	32.0	41.0
23	36.856582372438424	39.0	35.0	40.0	31.0	41.0
24	36.81561763508683	39.0	35.0	40.0	31.0	41.0
25	36.726556273495035	38.0	35.0	40.0	31.0	41.0
26	36.64065209732204	38.0	35.0	40.0	31.0	41.0
27	36.642102418684516	38.0	35.0	40.0	31.0	41.0
28	36.38836117106609	38.0	35.0	40.0	30.0	41.0
29	36.30627777074661	38.0	35.0	40.0	30.0	41.0
30	36.22382836951726	38.0	35.0	40.0	30.0	41.0
31	36.296847261321226	38.0	35.0	40.0	30.0	41.0
32	35.85430085274791	38.0	35.0	40.0	29.0	41.0
33	35.50387379467691	38.0	35.0	40.0	26.0	41.0
34	35.37097441756257	38.0	35.0	40.0	25.0	41.0
35	35.212400247649214	38.0	35.0	40.0	24.0	41.0
36	35.16030839852368	38.0	35.0	40.0	24.0	41.0
37	35.07113073757735	38.0	35.0	40.0	23.0	41.0
38	35.02631443924898	38.0	35.0	40.0	23.0	41.0
39	34.84375523774666	38.0	34.0	40.0	22.0	41.0
40	34.6547174780827	38.0	34.0	40.0	22.0	41.0
41	34.69331860208176	38.0	34.0	40.0	21.0	41.0
42	34.75006926652733	38.0	34.0	40.0	22.0	41.0
43	34.776322135529796	38.0	34.0	40.0	21.0	41.0
44	34.65919500323244	38.0	34.0	40.0	21.0	41.0
45	34.769621240366824	38.0	34.0	40.0	22.0	41.0
46	34.49998802800762	38.0	34.0	40.0	21.0	41.0
47	34.309041590701526	38.0	34.0	40.0	21.0	41.0
48	34.28030538842274	38.0	34.0	40.0	20.0	41.0
49	34.33392623200353	38.0	34.0	40.0	20.0	41.0
50	34.12762827989834	38.0	33.0	40.0	20.0	41.0
51	32.87759150877889	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	1.0
12	2.0
13	3.0
14	4.0
15	16.0
16	20.0
17	55.0
18	114.0
19	280.0
20	637.0
21	1139.0
22	1914.0
23	2603.0
24	3347.0
25	4225.0
26	5439.0
27	5783.0
28	5578.0
29	5774.0
30	6358.0
31	8090.0
32	10801.0
33	14567.0
34	20538.0
35	23872.0
36	25488.0
37	33815.0
38	55403.0
39	56463.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.926984528765274	14.80114520658528	17.11002945110125	14.16184081354819
2	28.48718483730062	41.856137698435774	17.164416502194296	12.492260962069308
3	21.033764439077952	42.313809864237605	25.470242757799756	11.18218293888469
4	19.41241461404007	17.55059877064741	49.12347912939671	13.913507485915805
5	35.74939541438486	20.016145086865357	26.702673858983612	17.53178563976617
6	17.771567544270717	42.04119049492216	28.717731204827107	11.46951075598001
7	56.02755610588714	4.863707418188535	33.754177370197944	5.354559105726375
8	52.442799530697904	20.975956818733774	19.38607623080633	7.195167419761997
9	48.885407509517734	5.9059548690093004	18.08181317534864	27.126824446124324
10	37.27189078806495	18.29046789966786	27.11451039682024	17.32313091544695
11	30.709528679762887	18.82886549979648	30.847035563658505	19.614570256782134
12	21.394976551997782	15.473287064433263	35.43025630325399	27.701480080314965
13	22.73926026769375	18.655442638763944	44.519050860444196	14.086246233098112
14	18.907538592572575	30.75912693390434	30.06680371747466	20.26653075604842
15	12.698179230987622	21.171613379898684	46.51563713233156	19.614570256782134
16	14.433091955163176	24.106803854297432	29.605368925496585	31.854735265042812
17	14.892474405590578	26.30759811047755	36.9483049369076	21.851622547024277
18	15.402823337859886	23.858128469739935	34.573403705844726	26.16564448655545
19	15.003642906252459	26.785793691786186	30.661982767172113	27.548580634789243
20	18.001771854872086	25.845821261574354	39.97243021183585	16.179976671717707
21	16.63354415441818	34.636342180065604	32.131117260534495	16.598996404981715
22	14.068117216067098	21.586528430061332	36.99756113412394	27.34779321974763
23	16.3650294682041	30.805988732644884	33.53149831194908	19.29748348720194
24	19.546158871759438	24.20292185025432	28.918176562943604	27.332742715042635
25	14.080431265371182	38.40649360866635	26.939719308087252	20.57335581787521
26	14.745389927791782	23.321783211162	34.56279994116621	27.370026919880008
27	21.69153990607117	26.583980105969236	26.785793691786186	24.938686296173408
28	12.662263253850705	25.994616023998713	36.30250146229336	25.040619259857227
29	18.840495435250336	26.708830883635652	28.934937352274165	25.515736328839843
30	14.606514816195713	31.477104419717527	29.76203099719855	24.154349766888206
31	19.089512876732943	23.01598432011055	23.595770808177896	34.2987319949786
32	20.81826857625646	34.73417046064806	24.195396597901823	20.252164365193657
33	15.902226448525564	26.574402512066058	22.96775429366955	34.555616745738824
34	18.255920150231404	28.161888701517707	25.665215205114432	27.916975943136457
35	14.288059818915064	24.501537545878385	29.27665222046253	31.933750414744022
36	14.598989563843215	29.579714656113072	23.35291039134733	32.46838538869638
37	13.640888116600363	26.443394709747597	31.167884959414945	28.747832214237096
38	14.677662656619313	28.346257384153873	23.705229024214212	33.270850935012604
39	18.67562399734564	29.27459987891185	25.183256997629545	26.86651912611297
40	15.606347208302406	27.26604161464551	33.793513916586	23.334097260466084
41	15.06076641274641	24.354795125004703	23.924145456286837	36.66029300596205
42	15.731540042893938	21.365559656438023	39.50039165517926	23.40250864548878
43	19.863929755189858	22.731735015341254	25.095690424800495	32.30864480466839
44	19.0908811044334	21.25404909885103	30.329845492886925	29.325224303828644
45	16.421810917772937	19.552657953336595	29.520880864993554	34.50465026389692
46	26.224136220749855	25.95288507913487	25.221225316317142	22.601753383798133
47	13.321406948544375	21.69564458917253	41.671084901949385	23.311863560333713
48	19.038888451816153	24.27680614607883	26.83607605977787	29.848229342327148
49	16.484407335068703	17.913179111267695	39.80174380620423	25.80066974745937
50	19.85811478746293	18.588057424516588	32.12256583740666	29.43126195061382
51	18.108835672432605	19.337504147440217	27.334110942743088	35.219549237384086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	422.0
1	538.5
2	655.0
3	3275.0
4	5895.0
5	3893.0
6	1891.0
7	1847.0
8	1803.0
9	1773.0
10	1743.0
11	1757.5
12	1772.0
13	1664.0
14	1556.0
15	1519.0
16	1482.0
17	1467.5
18	1453.0
19	1442.0
20	1431.0
21	1405.5
22	1380.0
23	1314.5
24	1249.0
25	1338.5
26	1816.0
27	2204.0
28	2261.5
29	2319.0
30	2662.5
31	3006.0
32	3566.0
33	4126.0
34	4719.0
35	5312.0
36	5937.0
37	6562.0
38	6992.0
39	7422.0
40	8350.0
41	9278.0
42	10473.0
43	11668.0
44	14207.0
45	16746.0
46	47115.0
47	77484.0
48	55667.5
49	33851.0
50	31484.0
51	29117.0
52	23037.0
53	16957.0
54	14694.5
55	12432.0
56	10673.0
57	8914.0
58	7687.5
59	6461.0
60	5592.5
61	4724.0
62	4054.0
63	3384.0
64	2846.5
65	2309.0
66	1832.0
67	1355.0
68	1141.0
69	927.0
70	814.0
71	701.0
72	555.0
73	409.0
74	325.0
75	200.0
76	159.0
77	107.0
78	55.0
79	43.0
80	31.0
81	28.0
82	25.0
83	15.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	292349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.997111884922205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66380192892773	27.993421456031314
2	10.420198703948405	7.71034514626201
3	3.9972168298393664	4.436564348455833
4	2.174277147569231	3.2176789958979337
5	1.473150831763802	2.7251163073065796
6	1.1174383889547677	2.480519586040005
7	0.8029482477799114	2.079473631264093
8	0.711399880434614	2.1055792777087756
9	0.575087945066834	1.9148933742576886
>10	2.964983992083482	16.581741459456413
>50	0.05472285699752817	1.4138984024697396
>100	0.026863947980604735	2.3665589246232637
>500	0.006964727254230858	1.7982019515919494
>1k	0.007959688290549551	7.94043281428658
>5k	0.0	0.0
>10k+	0.0029848831089560822	15.235574324347839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	14741	5.04226113309777	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	13471	4.607848838203654	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	13177	4.507284102220291	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3776	1.2916069492284905	No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	3515	1.2023300917738728	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	3065	1.0484044754728081	No Hit
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	2744	0.938604202511382	TruSeq Adapter, Index 13 (100% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT	2578	0.8818227529425448	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA	2331	0.7973346924395158	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	2285	0.7816000738842959	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	1277	0.4368066933699106	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	954	0.3263223065582574	TruSeq Adapter, Index 13 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	773	0.264410003112718	TruSeq Adapter, Index 13 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	741	0.25346418150908673	TruSeq Adapter, Index 13 (100% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG	696	0.23807161987898026	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT	596	0.2038659273676325	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT	578	0.1977089027155899	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	547	0.18710513803707213	TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	493	0.16863406408094433	TruSeq Adapter, Index 13 (100% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	492	0.16829200715583087	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACCAACTC	488	0.16692377945537695	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	424	0.14503213624811442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	376	0.1286134038426675	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	357	0.12211432226551143	TruSeq Adapter, Index 13 (100% over 21bp)
GGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	335	0.11458906991301493	No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	299	0.10227502060892973	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACCAACTCTCG	298	0.10193296368381626	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	297	0.10159090675870279	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.420569251134774E-4	0.0	0.0	0.35197657594176823	0.0
2	3.420569251134774E-4	0.0	0.0	2.1522221728139996	0.0
3	3.420569251134774E-4	0.0	0.0	2.7501376779123583	0.0
4	3.420569251134774E-4	0.0	0.0	4.426216610968398	0.0
5	3.420569251134774E-4	0.0	0.0	10.66294052656243	0.0
6	3.420569251134774E-4	0.0	0.0	12.39409062456174	0.0
7	3.420569251134774E-4	0.0	0.0	14.594200766891626	0.0
8	3.420569251134774E-4	0.0	0.0	16.786101543018788	0.0
9	3.420569251134774E-4	0.0	0.0	17.461321913192794	0.0
10	3.420569251134774E-4	0.0	0.0	23.44150313495172	0.0
11	3.420569251134774E-4	0.0	0.0	25.152471874369333	0.0
12	3.420569251134774E-4	0.0	0.0	31.735015341253092	0.0
13	3.420569251134774E-4	0.0	0.0	32.669172803738	0.0
14	3.420569251134774E-4	0.0	0.0	33.151473068148	0.0
15	3.420569251134774E-4	0.0	0.0	34.377747144679816	0.0
16	3.420569251134774E-4	0.0	0.0	35.30403729788711	0.0
17	3.420569251134774E-4	0.0	0.0	36.28539861603768	0.0
18	3.420569251134774E-4	0.0	0.0	37.24931503100746	0.0
19	3.420569251134774E-4	0.0	0.0	38.870664856045344	0.0
20	3.420569251134774E-4	0.0	0.0	39.633451799048395	0.0
21	3.420569251134774E-4	0.0	0.0	40.283702013689116	0.0
22	6.841138502269548E-4	0.0	0.0	41.03178050891229	0.0
23	6.841138502269548E-4	0.0	0.0	41.72855046536845	0.0
24	6.841138502269548E-4	0.0	0.0	42.26557983779661	0.0
25	6.841138502269548E-4	0.0	0.0	42.745485703730814	0.0
26	6.841138502269548E-4	0.0	0.0	43.23394299279286	0.0
27	6.841138502269548E-4	0.0	0.0	43.84861928722178	0.0
28	6.841138502269548E-4	0.0	0.0	44.28918860676794	0.0
29	6.841138502269548E-4	0.0	0.0	44.8009057667377	0.0
30	6.841138502269548E-4	0.0	0.0	45.341013651491885	0.0
31	6.841138502269548E-4	0.0	0.0	45.81202603737314	0.0
32	6.841138502269548E-4	0.0	0.0	46.2755131709019	0.0
33	6.841138502269548E-4	0.0	0.0	46.73592179210464	0.0
34	6.841138502269548E-4	0.0	0.0	47.17033408699876	0.0
35	6.841138502269548E-4	0.0	0.0	47.626638025100135	0.0
36	6.841138502269548E-4	0.0	0.0	48.03608016446097	0.0
37	0.0010261707753404321	0.0	0.0	48.44996904384828	0.0
38	0.0010261707753404321	0.0	0.0	48.89635333112137	0.0
39	0.0010261707753404321	0.0	0.0	49.398150840262836	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.883645E-5	45.000004	13
ACCGGCT	25	3.883645E-5	45.000004	34
AACCCTC	30	2.159928E-6	45.000004	39
ACGGCTA	60	0.0	45.000004	30
TCTAAAG	25	3.883645E-5	45.000004	24
CGGCTAT	50	2.1827873E-11	45.000004	31
TGCTAGG	30	2.159928E-6	45.000004	2
CACAACG	25	3.883645E-5	45.000004	12
CACCGGT	25	3.883645E-5	45.000004	16
CGTTTTC	25	3.883645E-5	45.000004	1
ATCCTGC	20	7.024648E-4	45.0	20
ACCGGTG	20	7.024648E-4	45.0	17
CTCGTTC	20	7.024648E-4	45.0	15
CGACGAA	20	7.024648E-4	45.0	19
CTATCCC	20	7.024648E-4	45.0	34
GTCCGCT	20	7.024648E-4	45.0	26
CCGACTT	35	1.2077726E-7	45.0	44
GGATTCA	20	7.024648E-4	45.0	8
CCTATCA	20	7.024648E-4	45.0	38
CGTTTTT	2060	0.0	44.344658	1
>>END_MODULE