SRR2935391.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179015
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3270	1.8266625701756836	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	1931	1.078680557495182	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	1912	1.0680669217663326	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	1708	0.9541099907828954	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	674	0.3765047621707678	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTG	465	0.25975476915342294	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	420	0.23461721084825296	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	412	0.2301483115940005	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	408	0.2279138619668743	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	400	0.22344496271262185	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC	394	0.22009328827193253	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	295	0.16479066000055861	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	292	0.16311482278021397	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	284	0.15864592352596152	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	281	0.15697008630561685	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	278	0.15529424908527217	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	249	0.1390944892886071	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	205	0.11451554339021869	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	196	0.1094880317291847	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	187	0.10446052006815071	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	20	7.017318E-4	45.000004	3
CACGGGT	20	7.017318E-4	45.000004	17
CAACGAG	20	7.017318E-4	45.000004	14
TCTAGCG	20	7.017318E-4	45.000004	1
GCACCAC	20	7.017318E-4	45.000004	29
TGACCGG	20	7.017318E-4	45.000004	2
AGCGACT	20	7.017318E-4	45.000004	19
GCGATGT	40	6.7593646E-9	45.000004	9
TTACGGG	40	6.7593646E-9	45.000004	3
CGATGCA	20	7.017318E-4	45.000004	10
CGGTAGG	20	7.017318E-4	45.000004	2
CACAACG	20	7.017318E-4	45.000004	12
CCTGCAC	20	7.017318E-4	45.000004	26
CGTTTCT	20	7.017318E-4	45.000004	1
TAGTCGG	20	7.017318E-4	45.000004	2
ACAAGCG	20	7.017318E-4	45.000004	14
GTAGTGG	20	7.017318E-4	45.000004	2
GTAGTCG	20	7.017318E-4	45.000004	1
TGGACGG	25	3.8775757E-5	45.0	2
CCTCGTG	25	3.8775757E-5	45.0	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content