##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179015
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647269781861855	31.0	31.0	34.0	30.0	34.0
2	31.731095159623496	33.0	31.0	34.0	30.0	34.0
3	31.783023768957907	33.0	31.0	34.0	30.0	34.0
4	35.568455157389046	37.0	35.0	37.0	33.0	37.0
5	35.59846940200542	37.0	35.0	37.0	33.0	37.0
6	35.55812082786359	37.0	35.0	37.0	33.0	37.0
7	35.85199005669916	37.0	35.0	37.0	35.0	37.0
8	35.60736251152138	37.0	35.0	37.0	33.0	37.0
9	37.40596039438036	39.0	37.0	39.0	34.0	39.0
10	36.7990280144122	39.0	37.0	39.0	32.0	39.0
11	36.86649722090328	39.0	37.0	39.0	32.0	39.0
12	37.01123928162445	39.0	37.0	39.0	33.0	39.0
13	36.986397787894866	39.0	37.0	39.0	33.0	39.0
14	38.031891182303156	40.0	37.0	41.0	33.0	41.0
15	38.10438790045527	40.0	37.0	41.0	33.0	41.0
16	38.057257771695106	40.0	37.0	41.0	33.0	41.0
17	38.02561237885094	40.0	37.0	41.0	33.0	41.0
18	37.82000391028685	39.0	37.0	41.0	33.0	41.0
19	37.549155098734744	39.0	37.0	40.0	33.0	41.0
20	37.41653492724073	39.0	35.0	40.0	33.0	41.0
21	37.39345306259252	39.0	35.0	40.0	33.0	41.0
22	37.52386112895567	39.0	35.0	41.0	33.0	41.0
23	37.5011367762478	39.0	35.0	41.0	33.0	41.0
24	37.46492193391615	39.0	35.0	41.0	33.0	41.0
25	37.24431472222998	39.0	35.0	40.0	33.0	41.0
26	37.32957573387705	39.0	35.0	41.0	33.0	41.0
27	37.35207105549814	39.0	35.0	41.0	33.0	41.0
28	37.22696980699941	39.0	35.0	41.0	33.0	41.0
29	37.18564924726978	39.0	35.0	41.0	33.0	41.0
30	36.98524146021283	39.0	35.0	40.0	32.0	41.0
31	36.863882914839536	39.0	35.0	40.0	32.0	41.0
32	36.55151802921543	39.0	35.0	41.0	31.0	41.0
33	36.103253917269505	39.0	35.0	41.0	30.0	41.0
34	35.77318101835042	39.0	35.0	41.0	26.0	41.0
35	35.46735748400972	39.0	35.0	41.0	24.0	41.0
36	35.28938915733318	39.0	35.0	41.0	23.0	41.0
37	35.16069044493478	39.0	35.0	41.0	22.0	41.0
38	35.06674301036226	39.0	35.0	40.0	22.0	41.0
39	35.00036868418847	39.0	35.0	40.0	21.0	41.0
40	34.96210373432394	39.0	35.0	40.0	21.0	41.0
41	34.821450716420415	39.0	35.0	40.0	20.0	41.0
42	34.856319302851716	39.0	35.0	40.0	20.0	41.0
43	34.7683937100243	39.0	35.0	40.0	19.0	41.0
44	34.63697455520487	39.0	34.0	40.0	18.0	41.0
45	34.59740803843253	38.0	34.0	40.0	20.0	41.0
46	34.452006815071364	38.0	34.0	40.0	20.0	41.0
47	34.37759405636399	38.0	34.0	40.0	19.0	41.0
48	34.303203642152894	38.0	34.0	40.0	18.0	41.0
49	34.243789626567605	38.0	34.0	40.0	18.0	41.0
50	34.11574449068514	38.0	34.0	40.0	18.0	41.0
51	32.93302237242689	37.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	4.0
14	4.0
15	10.0
16	9.0
17	42.0
18	44.0
19	137.0
20	261.0
21	367.0
22	574.0
23	865.0
24	1303.0
25	2291.0
26	3869.0
27	4478.0
28	4103.0
29	3691.0
30	3780.0
31	3986.0
32	5173.0
33	6582.0
34	10086.0
35	12608.0
36	14517.0
37	20711.0
38	36493.0
39	43014.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.39471552663185	20.351367203865596	23.54551294584253	19.708404323660027
2	37.389045610703015	26.59330223724269	22.07636231600704	13.941289836047257
3	26.73072088931095	25.082814289305368	34.37142138926906	13.815043432114626
4	23.910845459877663	22.219925704549897	37.92196184677262	15.947266988799822
5	24.525877719744155	25.557076222662907	32.79334134011116	17.123704717481775
6	21.31888389241125	33.06259252017988	33.14526715638355	12.473256431025332
7	67.3077675055163	5.610703013713934	22.09535513783761	4.9861743429321566
8	67.17872803954977	8.196519844705751	19.048683071251013	5.576069044493479
9	61.112755914308856	7.829511493450269	20.836242772951987	10.221489819288887
10	31.444850990140495	26.815071362734965	27.870848811552108	13.86922883557244
11	23.756668435605953	22.480797698516884	36.18914616093624	17.573387704940927
12	20.73680976454487	20.013406697762758	39.57657179565958	19.67321174203279
13	20.311705722984108	21.226154232885513	42.6014579783817	15.86068206574868
14	17.213641314973604	26.20562522693629	38.029774041281456	18.550959416808645
15	15.728849537748232	23.576236628215515	43.46898304611345	17.2259307879228
16	17.05052649219339	25.158785576627658	37.03656118202385	20.7541267491551
17	17.573387704940927	24.89735497025389	36.959472669888	20.569784654917186
18	18.16719269334972	24.475602603133815	37.868893668128365	19.488311035388094
19	17.705220232941375	26.44638717425914	36.25115213808899	19.597240454710498
20	18.144848197078456	26.16540513364802	37.61863530989023	18.07111135938329
21	17.756612574365278	28.740608328910984	36.84663296371812	16.656146133005613
22	16.785185598972152	24.177862190319246	36.65782197022595	22.37913024048264
23	16.286344719716226	27.003882356227134	36.45113537971678	20.258637544339862
24	17.36558388961819	24.232047593777057	36.38912940256403	22.013239114040722
25	16.409239449208165	28.67860235175823	34.004413038013574	20.907745161020024
26	17.09968438399017	25.33642432198419	36.246683238834734	21.317208055190907
27	19.322961762980757	25.91905706225735	34.782001508253494	19.975979666508394
28	17.260006144736476	24.328128927743485	37.8074463033824	20.60441862413764
29	20.691562159595563	23.471217495740582	34.79484959360947	21.04237075105438
30	20.098315783593552	25.617964975002096	35.996983493003384	18.28673574840097
31	22.877971119738568	25.01745663771192	32.31963801916041	19.7849342233891
32	21.26190542691953	27.95631650978968	31.98782224953216	18.793955813758622
33	19.70225958718543	26.611177834259696	32.38890595760132	21.29765662095355
34	23.501382565706784	25.287825042594196	31.456581850682902	19.754210541016114
35	20.0999916208139	25.700080998798985	31.86101723319275	22.33891014719437
36	22.814847917772255	26.224618048766864	32.5620758037036	18.398458229757285
37	20.40946289417088	27.24911320280423	33.56646091109683	18.774962991928053
38	19.663715331117505	28.817696841046843	30.465603441052423	21.05298438678323
39	21.97581208278636	27.283188559617912	31.12029718180041	19.620702175795323
40	20.888752339189452	26.126860877580093	32.03139401726112	20.952992765969334
41	20.547998771052704	25.007960226796637	32.144233723431	22.299807278719662
42	20.656369577968327	23.768957908555148	34.10105298438678	21.473619529089742
43	20.665307376476832	24.335949501438428	34.079825712929086	20.918917409155657
44	21.16805854258023	25.19230232103455	31.491215819903363	22.148423316481857
45	20.821718850375667	24.313605005167165	30.761109404239868	24.1035667402173
46	23.365081138452084	25.441443454459122	30.008658492305113	21.184816914783678
47	19.020193838505154	23.835991397368936	35.600368684188474	21.543446079937436
48	19.7123146105075	23.9097282350641	32.93020137977265	23.447755774655754
49	20.08826076027149	21.856269027735106	35.342848364662174	22.71262184733123
50	19.71566628494819	21.865765438650392	34.12116303103092	24.2974052453705
51	19.607295478032565	21.476971203530432	31.435354579225205	27.480378739211798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	205.0
1	228.0
2	251.0
3	2814.5
4	5378.0
5	3684.0
6	1990.0
7	1956.0
8	1922.0
9	1891.5
10	1861.0
11	1802.0
12	1743.0
13	1687.0
14	1631.0
15	1589.5
16	1548.0
17	1454.0
18	1360.0
19	1273.5
20	1187.0
21	1169.0
22	1151.0
23	1168.5
24	1186.0
25	1367.5
26	1804.0
27	2059.0
28	2194.0
29	2329.0
30	2725.5
31	3122.0
32	3654.0
33	4186.0
34	4374.5
35	4563.0
36	5182.5
37	5802.0
38	6210.0
39	6618.0
40	7246.0
41	7874.0
42	8821.0
43	9768.0
44	10908.0
45	12048.0
46	18072.0
47	24096.0
48	22176.5
49	20257.0
50	19104.0
51	17951.0
52	14558.5
53	11166.0
54	9201.0
55	7236.0
56	6119.0
57	5002.0
58	4279.5
59	3557.0
60	3237.0
61	2917.0
62	2524.5
63	2132.0
64	1726.5
65	1321.0
66	1078.0
67	835.0
68	640.0
69	445.0
70	384.5
71	324.0
72	244.0
73	164.0
74	149.5
75	96.5
76	58.0
77	57.0
78	56.0
79	36.0
80	16.0
81	14.0
82	12.0
83	7.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	179015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.234086529061805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07804023211644	42.09647236265118
2	9.033862556009108	9.618188419964808
3	3.6244582725584227	5.7883417590704695
4	2.1249357272526943	4.524760494930592
5	1.4544004533196218	3.8711839789961733
6	1.031512009821925	3.294695975197609
7	0.7859638813393915	2.928804848755691
8	0.5939326526543334	2.5293969779068792
9	0.4837508001301195	2.31768287573667
>10	1.731429111094788	13.406697762757311
>50	0.028332476363369257	1.0110884562746139
>100	0.02413507245768492	3.3086612853671484
>500	0.0010493509764210834	0.3765047621707678
>1k	0.004197403905684334	4.927520040220093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3270	1.8266625701756836	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	1931	1.078680557495182	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	1912	1.0680669217663326	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	1708	0.9541099907828954	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	674	0.3765047621707678	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTG	465	0.25975476915342294	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	420	0.23461721084825296	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	412	0.2301483115940005	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	408	0.2279138619668743	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	400	0.22344496271262185	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC	394	0.22009328827193253	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	295	0.16479066000055861	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	292	0.16311482278021397	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	284	0.15864592352596152	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	281	0.15697008630561685	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	278	0.15529424908527217	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	249	0.1390944892886071	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	205	0.11451554339021869	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	196	0.1094880317291847	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	187	0.10446052006815071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011172248135631092	0.0	0.0	0.25081697064491804	0.0
2	0.0011172248135631092	0.0	0.0	1.1105214646817305	0.0
3	0.0011172248135631092	0.0	0.0	1.6914783677345473	0.0
4	0.0011172248135631092	0.0	0.0	2.6651397927547973	0.0
5	0.0011172248135631092	0.0	0.0	5.3179901125604	0.0
6	0.0011172248135631092	0.0	0.0	6.890484037650476	0.0
7	0.0011172248135631092	0.0	0.0	8.540066474876406	0.0
8	0.0011172248135631092	0.0	0.0	10.63262855068011	0.0
9	0.0011172248135631092	0.0	0.0	11.578359355361282	0.0
10	0.0011172248135631092	0.0	0.0	14.231768287573667	0.0
11	0.0011172248135631092	0.0	0.0	16.42097030975058	0.0
12	0.0011172248135631092	0.0	0.0	19.148674692064912	0.0
13	0.0011172248135631092	0.0	0.0	19.968717705220232	0.0
14	0.0011172248135631092	0.0	0.0	20.353043041085943	0.0
15	0.0011172248135631092	0.0	0.0	20.930089657291287	0.0
16	0.0011172248135631092	0.0	0.0	21.96128816021004	0.0
17	0.0011172248135631092	0.0	0.0	23.040527330112003	0.0
18	0.0011172248135631092	0.0	0.0	24.288467446861993	0.0
19	0.0011172248135631092	0.0	0.0	25.15710973940731	0.0
20	0.0011172248135631092	0.0	0.0	25.9145881630031	0.0
21	0.0011172248135631092	0.0	0.0	26.735748400971985	0.0
22	0.0011172248135631092	0.0	0.0	27.580370360025697	0.0
23	0.0011172248135631092	0.0	0.0	28.4221992570455	0.0
24	0.0011172248135631092	0.0	0.0	29.138340362539452	0.0
25	0.0011172248135631092	0.0	0.0	29.76231042091445	0.0
26	0.0011172248135631092	0.0	0.0	30.32259866491635	0.0
27	0.0011172248135631092	0.0	0.0	30.93092757590146	0.0
28	0.0011172248135631092	0.0	0.0	31.51244309136106	0.0
29	0.0011172248135631092	0.0	0.0	32.09507583163422	0.0
30	0.0011172248135631092	0.0	0.0	32.77546574309415	0.0
31	0.0011172248135631092	0.0	0.0	33.40781498757087	0.0
32	0.0011172248135631092	0.0	0.0	34.02452308465771	0.0
33	0.0011172248135631092	0.0	0.0	34.58872161550708	0.0
34	0.0011172248135631092	0.0	0.0	35.1484512471022	0.0
35	0.0011172248135631092	0.0	0.0	35.68360193279893	0.0
36	0.0011172248135631092	0.0	0.0	36.22489735497025	0.0
37	0.0011172248135631092	0.0	0.0	36.75557914141273	0.0
38	0.0011172248135631092	0.0	0.0	37.26279920677038	0.0
39	0.0011172248135631092	0.0	0.0	37.86386615646734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	20	7.017318E-4	45.000004	3
CACGGGT	20	7.017318E-4	45.000004	17
CAACGAG	20	7.017318E-4	45.000004	14
TCTAGCG	20	7.017318E-4	45.000004	1
GCACCAC	20	7.017318E-4	45.000004	29
TGACCGG	20	7.017318E-4	45.000004	2
AGCGACT	20	7.017318E-4	45.000004	19
GCGATGT	40	6.7593646E-9	45.000004	9
TTACGGG	40	6.7593646E-9	45.000004	3
CGATGCA	20	7.017318E-4	45.000004	10
CGGTAGG	20	7.017318E-4	45.000004	2
CACAACG	20	7.017318E-4	45.000004	12
CCTGCAC	20	7.017318E-4	45.000004	26
CGTTTCT	20	7.017318E-4	45.000004	1
TAGTCGG	20	7.017318E-4	45.000004	2
ACAAGCG	20	7.017318E-4	45.000004	14
GTAGTGG	20	7.017318E-4	45.000004	2
GTAGTCG	20	7.017318E-4	45.000004	1
TGGACGG	25	3.8775757E-5	45.0	2
CCTCGTG	25	3.8775757E-5	45.0	15
>>END_MODULE