##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317864
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851971283316136	33.0	31.0	34.0	30.0	34.0
2	31.88219175496439	33.0	31.0	34.0	30.0	34.0
3	31.926282938615255	33.0	31.0	34.0	30.0	34.0
4	35.637014572269905	37.0	35.0	37.0	33.0	37.0
5	35.660291822917976	37.0	35.0	37.0	33.0	37.0
6	35.64536090906803	37.0	35.0	37.0	33.0	37.0
7	35.83497659376337	37.0	35.0	37.0	35.0	37.0
8	35.74353182493142	37.0	35.0	37.0	35.0	37.0
9	37.52698323811441	39.0	37.0	39.0	35.0	39.0
10	36.93184506581431	39.0	37.0	39.0	32.0	39.0
11	36.960759947650565	39.0	37.0	39.0	33.0	39.0
12	37.04563901542798	39.0	37.0	39.0	33.0	39.0
13	37.10349709309642	39.0	37.0	39.0	33.0	39.0
14	38.25839352679133	40.0	38.0	41.0	33.0	41.0
15	38.34276294264214	40.0	38.0	41.0	34.0	41.0
16	38.22703734930662	40.0	37.0	41.0	33.0	41.0
17	38.18782875695266	40.0	37.0	41.0	33.0	41.0
18	37.93987680265774	39.0	37.0	41.0	33.0	41.0
19	37.72540772154129	39.0	37.0	41.0	33.0	41.0
20	37.61373417562228	39.0	35.0	41.0	33.0	41.0
21	37.56522600860745	39.0	35.0	41.0	33.0	41.0
22	37.62355913220748	39.0	35.0	41.0	33.0	41.0
23	37.67567890670224	39.0	35.0	41.0	33.0	41.0
24	37.70452457654846	39.0	35.0	41.0	34.0	41.0
25	37.40529282963783	39.0	35.0	41.0	33.0	41.0
26	37.5398944202552	39.0	35.0	41.0	33.0	41.0
27	37.50771084488964	39.0	35.0	41.0	33.0	41.0
28	37.16990285153399	39.0	35.0	41.0	33.0	41.0
29	36.939291646742	39.0	35.0	41.0	32.0	41.0
30	36.802531900435405	39.0	35.0	41.0	31.0	41.0
31	36.772025142828376	39.0	35.0	41.0	31.0	41.0
32	36.599555155664056	39.0	35.0	41.0	31.0	41.0
33	36.10602018473309	39.0	35.0	41.0	28.0	41.0
34	35.74132018724989	39.0	35.0	41.0	25.0	41.0
35	35.55153776457856	39.0	35.0	41.0	23.0	41.0
36	35.37516673797599	39.0	35.0	41.0	23.0	41.0
37	35.28545541489442	39.0	35.0	41.0	22.0	41.0
38	35.10027873555986	39.0	35.0	41.0	21.0	41.0
39	35.002595449626256	39.0	35.0	41.0	20.0	41.0
40	34.941116326479246	39.0	35.0	41.0	20.0	41.0
41	34.78237862733748	39.0	35.0	41.0	19.0	41.0
42	34.81458107870032	39.0	35.0	41.0	19.0	41.0
43	34.68506342335087	39.0	35.0	41.0	18.0	41.0
44	34.61290363174188	39.0	34.0	41.0	18.0	41.0
45	34.640434273777466	39.0	34.0	41.0	20.0	41.0
46	34.445523871844564	39.0	34.0	40.0	18.0	41.0
47	34.47542345153902	38.0	34.0	40.0	19.0	41.0
48	34.38225467495533	38.0	34.0	40.0	18.0	41.0
49	34.13343757078499	38.0	34.0	40.0	15.0	41.0
50	33.97104107417009	38.0	34.0	40.0	15.0	41.0
51	32.86477550147233	37.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	3.0
11	1.0
12	2.0
13	1.0
14	7.0
15	14.0
16	16.0
17	61.0
18	110.0
19	245.0
20	457.0
21	783.0
22	1165.0
23	1719.0
24	2687.0
25	4486.0
26	6984.0
27	8201.0
28	7693.0
29	6962.0
30	6777.0
31	7073.0
32	8266.0
33	10556.0
34	16101.0
35	20222.0
36	22812.0
37	32480.0
38	60801.0
39	91134.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.02810006795359	20.255832683160094	22.814474114715726	19.90159313417059
2	36.82046409785317	25.28219615936375	23.11774847104422	14.779591271738857
3	27.43846424886115	24.20060151511338	34.471031636171446	13.889902599854025
4	23.916203156066747	23.782498175320264	36.24820678025822	16.053091888354768
5	23.409697229003598	27.181436085873205	32.78068607958121	16.628180605541992
6	21.849596053658168	32.887335464223696	32.598847305765986	12.66422117635215
7	71.87948304935445	5.437231017038734	18.61959831877784	4.063687614828983
8	73.32538444114464	6.001623335766239	16.701796994941233	3.971195228147887
9	67.10668713663705	8.048410641028868	18.48432033825787	6.360581884076209
10	31.14602471497244	31.934097601489945	24.724095839730197	12.195781843807413
11	22.378753177459544	26.186671029119374	35.406651901442125	16.02792389197896
12	21.265383937784712	21.112488359801677	39.523821508569704	18.098306193843907
13	22.504278559383888	22.260778194447937	38.88832960008054	16.346613646087636
14	17.09661993808673	28.43448770543377	36.8563284926887	17.612563863790804
15	16.494790224750208	25.561875519089927	41.25852565877231	16.684808597387562
16	18.467331940704202	26.970968716180504	36.0540986082098	18.507600734905495
17	19.451400598998315	25.112626783781742	34.0038507034455	21.432121913774445
18	22.100961417461555	24.568054262200185	34.87655097777666	18.4544333425616
19	21.795799461404876	25.788702086426902	33.650869554274784	18.76462889789344
20	20.739058213575618	25.10287418518612	34.55974882339617	19.598318777842096
21	22.006896031006974	26.34491480633227	35.323282913447265	16.324906249213498
22	18.87316588226411	25.82142048171545	34.994525960788266	20.310887675232177
23	17.63143986107266	25.462147333450787	35.36449550751264	21.54191729796391
24	17.95736541413938	24.477134875292577	35.390921903707245	22.174577806860796
25	17.842221830720057	27.808748395540235	33.73738454181663	20.611645231923088
26	17.646540658898143	26.110852440037245	33.901605718168774	22.34100118289583
27	19.48852339365263	25.67827750232804	34.05041149674074	20.782787607278586
28	19.661553368736314	25.69495129992701	36.49485314474114	18.148642186595527
29	19.222057232023758	24.158445121183902	37.53083079556036	19.088666851231974
30	22.054715224121008	25.467495532680644	34.61826441496992	17.859524828228423
31	20.020197317091586	28.46437470113004	32.481816122618476	19.033611859159894
32	19.720383560264768	29.600709737497798	32.65578989756626	18.02311680467118
33	20.688093020914604	26.965305916995945	33.16009362494652	19.18650743714293
34	21.00174917574812	26.92440792288526	32.03351118717439	20.040331714192234
35	19.059409055445094	27.961014773613872	31.24480910074749	21.73476707019354
36	19.825774545088464	28.399567110462336	31.791269222057235	19.983389122391966
37	19.400120806382603	30.648642186595527	30.397276822792136	19.553960184229734
38	19.405469005612463	28.907331437344276	29.487768353761357	22.199431203281907
39	22.138713412025268	27.59639342611935	29.329839176503157	20.935053985352226
40	22.8704729066519	25.69211990033474	30.785807766843682	20.651599426169685
41	19.12169984647522	27.128583293484006	30.659338585055245	23.09037827498553
42	20.62706062970327	27.40134145420683	30.80248156444265	21.169116351647247
43	19.869189338836733	28.5883270832809	30.445096015906177	21.09738756197619
44	19.50802859084388	27.566191830468377	29.952117886895024	22.973661691792717
45	19.614363375531674	26.087572043389624	29.77908791181134	24.51897666926736
46	20.28697807867516	28.172111343215967	29.240807389323738	22.30010318878514
47	18.856806684619837	25.23846676566078	32.06465658268945	23.840069967029926
48	18.976983867314324	25.611267711977447	31.561611255127982	23.85013716558025
49	20.309629275413386	25.14031157979513	32.65547529761156	21.894583847179927
50	18.876626481765786	24.440326680592957	34.284473863035764	22.39857297460549
51	20.036241914781165	24.352867893187025	31.31307729091687	24.29781290111494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	290.0
2	370.0
3	5150.0
4	9930.0
5	6781.5
6	3633.0
7	3492.5
8	3352.0
9	3340.0
10	3328.0
11	3185.0
12	3042.0
13	2967.0
14	2892.0
15	2738.5
16	2585.0
17	2365.5
18	2146.0
19	2058.5
20	1971.0
21	1994.0
22	2017.0
23	2131.5
24	2246.0
25	2482.0
26	3071.5
27	3425.0
28	4027.0
29	4629.0
30	5625.5
31	6622.0
32	7412.5
33	8203.0
34	9223.5
35	10244.0
36	11124.0
37	12004.0
38	12444.0
39	12884.0
40	14378.0
41	15872.0
42	16912.5
43	17953.0
44	19633.5
45	21314.0
46	26767.0
47	32220.0
48	30641.0
49	29062.0
50	27582.5
51	26103.0
52	22128.5
53	18154.0
54	16168.5
55	14183.0
56	13068.0
57	11953.0
58	11063.0
59	10173.0
60	9466.0
61	8759.0
62	7338.0
63	5917.0
64	4882.5
65	3848.0
66	2763.5
67	1679.0
68	1322.5
69	966.0
70	744.5
71	523.0
72	441.0
73	359.0
74	283.5
75	143.5
76	79.0
77	60.0
78	41.0
79	33.5
80	26.0
81	20.0
82	14.0
83	9.0
84	4.0
85	2.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.877173796645046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57791310396586	37.14211228459601
2	8.26561262682655	7.914683445406371
3	3.48205035037798	5.001321893811459
4	2.1870858301076335	4.188459535849715
5	1.558225448933307	3.7301715316467594
6	1.1844082088894703	3.402367059790454
7	0.9721011442133446	3.257901880059387
8	0.7901461475608962	3.0263971545218054
9	0.6710705479793445	2.891606512987522
>10	3.2698723970084402	23.04433275631951
>50	0.02240448572484592	0.7713712211924245
>100	0.013179109249909364	1.3354660038067618
>500	0.002635821849981873	0.8243734155320266
>1k	0.002635821849981873	1.688498476818755
>5k	6.589554624954683E-4	1.7809368276610373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5645	1.7759167442679888	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	2215	0.6968388996551985	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	1068	0.33599275161704373	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	1057	0.33253215211537007	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	1012	0.3183751541539778	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG	837	0.2633201620818966	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	760	0.23909596557018095	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	513	0.16138977675987215	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	503	0.15824377721289606	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	494	0.15541237762061763	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	369	0.11608738328341682	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	365	0.11482898346462639	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	318	0.10004278559383886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1459995469760655E-4	0.0	0.0	0.1679963758085219	0.0
2	3.1459995469760655E-4	0.0	0.0	0.6965242997005009	0.0
3	3.1459995469760655E-4	0.0	0.0	1.1744016308861651	0.0
4	3.1459995469760655E-4	0.0	0.0	1.7387939496136713	0.0
5	3.1459995469760655E-4	0.0	0.0	3.243525532932323	0.0
6	3.1459995469760655E-4	0.0	0.0	4.524891148415675	0.0
7	3.1459995469760655E-4	0.0	0.0	5.353862029043868	0.0
8	3.1459995469760655E-4	0.0	0.0	6.461883069488838	0.0
9	3.1459995469760655E-4	0.0	0.0	6.961467797548637	0.0
10	6.291999093952131E-4	0.0	0.0	8.10315103314625	0.0
11	6.291999093952131E-4	0.0	0.0	9.044119497646792	0.0
12	6.291999093952131E-4	0.0	0.0	10.244947524727557	0.0
13	6.291999093952131E-4	0.0	0.0	10.812800442956735	0.0
14	6.291999093952131E-4	0.0	0.0	11.14092819570634	0.0
15	6.291999093952131E-4	0.0	0.0	11.431618553846928	0.0
16	6.291999093952131E-4	0.0	0.0	11.875519089925252	0.0
17	6.291999093952131E-4	0.0	0.0	12.473259003850703	0.0
18	6.291999093952131E-4	0.0	0.0	13.20596229834143	0.0
19	6.291999093952131E-4	0.0	0.0	13.653323433921425	0.0
20	6.291999093952131E-4	0.0	0.0	14.069224574031661	0.0
21	6.291999093952131E-4	0.0	0.0	14.519417109203935	0.0
22	6.291999093952131E-4	0.0	0.0	15.01585583771676	0.0
23	6.291999093952131E-4	0.0	0.0	15.459756373795082	0.0
24	6.291999093952131E-4	0.0	0.0	15.819029522059749	0.0
25	6.291999093952131E-4	0.0	0.0	16.19749326756097	0.0
26	6.291999093952131E-4	0.0	0.0	16.58633881156721	0.0
27	6.291999093952131E-4	0.0	0.0	16.97424055570936	0.0
28	6.291999093952131E-4	0.0	0.0	17.316839906375055	0.0
29	6.291999093952131E-4	0.0	0.0	17.689011652782323	0.0
30	6.291999093952131E-4	0.0	0.0	18.14046258777339	0.0
31	6.291999093952131E-4	0.0	0.0	18.534970930964185	0.0
32	6.291999093952131E-4	0.0	0.0	18.9537034706667	0.0
33	6.291999093952131E-4	0.0	0.0	19.43346840158055	0.0
34	6.291999093952131E-4	0.0	0.0	19.9528729267863	0.0
35	6.291999093952131E-4	0.0	0.0	20.40369466186797	0.0
36	6.291999093952131E-4	0.0	0.0	20.803551204288627	0.0
37	6.291999093952131E-4	0.0	0.0	21.17792515037878	0.0
38	6.291999093952131E-4	0.0	0.0	21.580298492437016	0.0
39	6.291999093952131E-4	0.0	0.0	22.044648025570684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	35	1.2082273E-7	45.000004	1
ACGCGAG	20	7.025578E-4	45.0	1
CGTTGCG	20	7.025578E-4	45.0	2
GTTAGCG	20	7.025578E-4	45.0	1
TCTAGCG	20	7.025578E-4	45.0	1
GTATGCG	20	7.025578E-4	45.0	1
ATTTGCG	20	7.025578E-4	45.0	1
TGCGTAG	20	7.025578E-4	45.0	1
GTACGAG	20	7.025578E-4	45.0	1
CGTTTTT	3520	0.0	43.977272	1
ACGGGAA	195	0.0	41.538464	5
AAGGGCG	55	6.002665E-11	40.909092	5
ATTGCGG	40	3.4477125E-7	39.375	2
GTTTTTT	3995	0.0	38.917397	2
CGCATGG	35	6.234035E-6	38.57143	2
ACGGGTC	35	6.234035E-6	38.57143	5
CGTTTCT	70	0.0	38.57143	1
CGATAGA	35	6.234035E-6	38.57143	10
AGACGGG	100	0.0	38.25	3
GACGTAG	30	1.13806454E-4	37.500004	1
>>END_MODULE