SRR2935386.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105166
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2514	2.3905064374417586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	1515	1.4405796550215848	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	1481	1.4082498145788562	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	1406	1.3369339900728372	No Hit
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	532	0.5058669151626951	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	427	0.40602476085426853	Illumina PCR Primer Index 11 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	391	0.3717931650913794	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	387	0.3679896544510583	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	356	0.3385124469885704	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	325	0.3090352395260826	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	291	0.27670539908335395	RNA PCR Primer, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	284	0.2700492554627921	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	284	0.2700492554627921	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	248	0.235817659699903	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC	245	0.23296502671966224	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC	238	0.22630888309910047	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTT	234	0.22250537245877947	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTA	221	0.21014396287773615	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	208	0.19778255329669284	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	173	0.16450183519388395	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	171	0.16260007987372344	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGT	158	0.15023867029268015	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	126	0.119810585170112	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125	0.11885970751003176	No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	123	0.11695795218987125	RNA PCR Primer, Index 14 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGGT	35	1.1978955E-7	45.000004	4
GGGACCG	30	2.1466712E-6	45.000004	7
TAGGGTC	30	2.1466712E-6	45.000004	5
TAGGGCC	35	1.1978955E-7	45.000004	5
AACCGAG	20	7.0040545E-4	45.0	1
ACCGGGA	20	7.0040545E-4	45.0	4
GTATTAG	25	3.8665945E-5	45.0	1
GGGTACT	20	7.0040545E-4	45.0	8
GACCGAT	25	3.8665945E-5	45.0	9
ACCGAGG	25	3.8665945E-5	45.0	2
ATGTAGG	25	3.8665945E-5	45.0	2
GGGCGAT	95	0.0	45.0	7
TAGGGTG	20	7.0040545E-4	45.0	5
CGAATGG	20	7.0040545E-4	45.0	2
CGGGAGT	20	7.0040545E-4	45.0	6
GCGATTG	20	7.0040545E-4	45.0	9
CACAGGG	20	7.0040545E-4	45.0	3
GGCGGGA	25	3.8665945E-5	45.0	4
CTACAGG	25	3.8665945E-5	45.0	2
TGGGCGA	25	3.8665945E-5	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content