##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935385.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	779796
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.863157800245194	33.0	31.0	34.0	30.0	34.0
2	31.91335041472385	33.0	31.0	34.0	30.0	34.0
3	32.00039369270937	33.0	31.0	34.0	30.0	34.0
4	35.72803143386219	37.0	35.0	37.0	33.0	37.0
5	35.74844959450933	37.0	35.0	37.0	35.0	37.0
6	35.699981790109206	37.0	35.0	37.0	35.0	37.0
7	35.98626179154548	37.0	35.0	37.0	35.0	37.0
8	35.82295113080857	37.0	35.0	37.0	35.0	37.0
9	37.675102206218035	39.0	37.0	39.0	35.0	39.0
10	36.94317744640906	39.0	37.0	39.0	32.0	39.0
11	36.87229865246808	39.0	37.0	39.0	33.0	39.0
12	37.02577725456401	39.0	37.0	39.0	33.0	39.0
13	37.037055845374944	39.0	37.0	39.0	33.0	39.0
14	38.121571539223076	40.0	37.0	41.0	33.0	41.0
15	38.23432154050546	40.0	38.0	41.0	33.0	41.0
16	38.16012136507497	40.0	37.0	41.0	33.0	41.0
17	38.14166012649462	40.0	37.0	41.0	33.0	41.0
18	38.0622290958148	40.0	37.0	41.0	33.0	41.0
19	37.9567630508492	40.0	37.0	41.0	33.0	41.0
20	38.0136240760404	40.0	37.0	41.0	33.0	41.0
21	37.98092065104207	40.0	37.0	41.0	33.0	41.0
22	38.0892784779609	40.0	37.0	41.0	34.0	41.0
23	38.07627764184479	40.0	37.0	41.0	34.0	41.0
24	38.04977455642245	40.0	37.0	41.0	34.0	41.0
25	37.83715228085294	40.0	36.0	41.0	33.0	41.0
26	37.91454431671873	40.0	37.0	41.0	33.0	41.0
27	37.920892130762404	40.0	37.0	41.0	33.0	41.0
28	37.84377196087182	40.0	37.0	41.0	33.0	41.0
29	37.83059159062114	40.0	37.0	41.0	33.0	41.0
30	37.68251824836239	40.0	36.0	41.0	33.0	41.0
31	37.703644286454406	40.0	37.0	41.0	33.0	41.0
32	37.665930576714935	40.0	37.0	41.0	33.0	41.0
33	37.569655397052564	40.0	37.0	41.0	33.0	41.0
34	37.56452969751063	40.0	37.0	41.0	33.0	41.0
35	37.516514575606955	40.0	37.0	41.0	33.0	41.0
36	37.433514662809245	40.0	37.0	41.0	33.0	41.0
37	37.39490456478361	40.0	37.0	41.0	33.0	41.0
38	37.33346541916091	40.0	36.0	41.0	32.0	41.0
39	37.28879476170691	40.0	36.0	41.0	32.0	41.0
40	37.17362489676787	40.0	36.0	41.0	32.0	41.0
41	37.06742147946386	40.0	36.0	41.0	31.0	41.0
42	37.114719747215936	40.0	36.0	41.0	32.0	41.0
43	37.04465142165387	40.0	36.0	41.0	32.0	41.0
44	36.924514873120664	40.0	35.0	41.0	31.0	41.0
45	36.9052175184279	39.0	35.0	41.0	31.0	41.0
46	36.79463090346706	39.0	35.0	41.0	31.0	41.0
47	36.71089746549097	39.0	35.0	41.0	31.0	41.0
48	36.63193963549441	39.0	35.0	41.0	31.0	41.0
49	36.566525091177695	39.0	35.0	41.0	31.0	41.0
50	36.42673211968258	39.0	35.0	41.0	31.0	41.0
51	35.260319365577665	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	11.0
11	11.0
12	17.0
13	27.0
14	26.0
15	35.0
16	49.0
17	99.0
18	159.0
19	342.0
20	644.0
21	1012.0
22	1544.0
23	2225.0
24	3226.0
25	4665.0
26	6323.0
27	7054.0
28	7727.0
29	8837.0
30	10832.0
31	13931.0
32	18282.0
33	25330.0
34	41805.0
35	50413.0
36	58177.0
37	88791.0
38	170785.0
39	257324.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83915793361341	20.61000569379684	24.536288978143002	11.014547394446753
2	32.456693801968726	25.92588317970341	24.763656135707286	16.853766882620583
3	31.444377760337318	25.40023288142027	26.77533611354764	16.380053244694768
4	27.51719680531831	25.23057312425301	29.047084109177273	18.205145961251404
5	26.6960846170024	29.099661962872343	25.148500377021684	19.055753043103582
6	25.527958594299022	35.69831084027105	24.98255954121334	13.79117102421659
7	79.80125571303263	5.273430486947869	10.671252481418216	4.254061318601275
8	81.43129741624733	6.413856957460669	8.078394862246023	4.0764507640459815
9	75.51551944354678	7.3757752027453325	9.653037461079563	7.455667892628329
10	36.29949371374052	33.583911689723976	17.05022852130557	13.066366075229933
11	25.616827990910444	27.211732299216717	29.039646266459435	18.131793443413404
12	23.652596320063196	24.486147659131362	31.9714386839635	19.889817336841944
13	23.473446901497315	25.236600341627813	33.455031828837285	17.83492092803759
14	20.384818593580885	29.304074398945367	30.099282376416397	20.211824631057354
15	19.2122042175133	28.43371856229065	33.239206151352406	19.114871068843648
16	22.183237667287344	27.659798203632747	30.184817567671544	19.97214656140837
17	22.752745589872223	26.847149767375058	29.77060667148844	20.62949797126428
18	22.16387362848745	26.86523141949946	30.40500335985309	20.56589159216
19	22.425865226289954	28.572472800578613	28.590810930038113	20.410851043093324
20	23.773781860896953	27.911274230696232	29.93795813263982	18.376985775766997
21	23.46177718275036	28.239180503618893	30.036701906652507	18.262340406978236
22	22.14450958968756	25.408055440140753	30.332676751355482	22.114758218816203
23	20.97343407763056	28.38614201662999	30.44514206279591	20.195281842943537
24	21.404316000595028	27.148510636115088	29.97578853956676	21.471384823723128
25	21.507291650636834	29.71943944313641	28.154158267033942	20.619110639192815
26	21.061790519571787	28.43615509697408	29.028104786380027	21.473949597074107
27	21.517935460043397	28.509251137476983	29.5496514473016	20.42316195517802
28	19.940728087858876	28.147233378986297	30.733550826113497	21.17848770704133
29	21.964590739116385	26.981415652298807	29.272655925395874	21.781337683188937
30	21.503444490610367	28.450133111736914	29.650318801327526	20.396103596325194
31	21.962795397770698	27.736228449491918	28.122355077481803	22.17862107525558
32	22.083339745266713	28.818819280940144	28.037461079564395	21.06037989422875
33	21.186310265761815	28.384218436616752	27.961672027042972	22.46779927057846
34	21.39046622449974	27.246997932792677	30.205079277144282	21.1574565655633
35	20.740039702691472	27.85933757033891	29.178785220750044	22.221837506219575
36	22.562182929894487	28.3788324125797	28.00476021933942	21.0542244381864
37	20.508825385100717	28.60209593278242	29.382171747482676	21.506906934634188
38	20.31698033844749	28.95423931387183	27.804451420627956	22.924328927052716
39	21.59782814992639	26.846123858034666	28.666599982559543	22.889448009479402
40	21.610652016681286	27.06643788888376	30.967714633057874	20.35519546137708
41	21.167202704297022	27.2808529410256	29.699562449666324	21.852381905011054
42	21.49793022790576	27.168131152250076	29.90730909109562	21.426629528748546
43	22.48485501336247	27.04463731540044	28.201350096691957	22.269157574545137
44	21.084873479730597	26.987827585676254	28.838696274410232	23.088602660182918
45	20.752350614776173	26.11490697567056	28.73918306839225	24.393559341161023
46	22.317118836208444	27.29226618243746	28.62364002893064	21.766974952423453
47	20.407388599069503	26.98975116568949	31.271640275148886	21.331219960092128
48	20.807749719157318	27.214040595232596	28.98501659408358	22.9931930915265
49	20.840194102047203	26.479104791509574	30.643142565491488	22.037558540951736
50	20.470482023503582	26.00385228957317	30.498361109828725	23.027304577094522
51	20.367634612129326	25.802389342853772	29.01976927298935	24.810206772027556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	409.0
1	674.0
2	939.0
3	2952.5
4	4966.0
5	3429.0
6	1892.0
7	1867.5
8	1843.0
9	1900.5
10	1958.0
11	1960.0
12	1962.0
13	2003.5
14	2045.0
15	2053.5
16	2062.0
17	2028.0
18	1994.0
19	2132.0
20	2270.0
21	2743.5
22	3217.0
23	3725.0
24	4233.0
25	5074.5
26	7216.0
27	8516.0
28	10529.5
29	12543.0
30	14343.0
31	16143.0
32	18360.0
33	20577.0
34	24147.0
35	27717.0
36	30713.5
37	33710.0
38	35094.0
39	36478.0
40	40866.5
41	45255.0
42	47796.5
43	50338.0
44	53918.5
45	57499.0
46	69807.5
47	82116.0
48	78655.5
49	75195.0
50	72838.0
51	70481.0
52	60975.0
53	51469.0
54	45196.0
55	38923.0
56	34933.0
57	30943.0
58	27652.5
59	24362.0
60	22598.5
61	20835.0
62	17816.0
63	14797.0
64	12287.5
65	9778.0
66	7792.5
67	5807.0
68	4897.0
69	3987.0
70	3315.5
71	2644.0
72	2191.0
73	1738.0
74	1395.5
75	855.0
76	657.0
77	461.5
78	266.0
79	215.0
80	164.0
81	99.0
82	34.0
83	32.0
84	30.0
85	18.5
86	7.0
87	10.0
88	13.0
89	8.0
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	5.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	779796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.6075712312711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75942133382244	23.39900442657411
2	8.878254151126464	5.789966092843691
3	3.5231933028694487	3.4464832975455857
4	2.1001515173169047	2.739233607894922
5	1.6867458504318473	2.750034273350371
6	1.2927366857219247	2.529180213777299
7	1.0773731819616308	2.459136594143255
8	0.9694237739435354	2.528844380972119
9	0.8924901474008886	2.619174244912391
>10	7.717268897593958	45.2030993292309
>50	0.06902184980854331	1.5075395395616105
>100	0.027608739071750474	1.580974501562465
>500	0.0035496950235107687	0.8131865104839711
>1k	0.002760873907175042	2.63414298714735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	4485	0.5751504239570349	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	4355	0.5584793971756716	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	4240	0.5437319504075425	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2889	0.3704815105489128	No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	2249	0.28840876331758564	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1216	0.15593821973952163	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1048	0.13439412359129824	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	920	0.1179795741450328	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	826	0.10592513939543162	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT	794	0.10182150203386527	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.129546701957948E-4	0.0	0.0	0.14593560367070363	0.0
2	5.129546701957948E-4	0.0	0.0	0.7827688267187829	0.0
3	5.129546701957948E-4	0.0	0.0	1.1142657823328153	0.0
4	5.129546701957948E-4	0.0	0.0	1.6017009576863692	0.0
5	5.129546701957948E-4	0.0	0.0	2.9684686764230643	0.0
6	5.129546701957948E-4	0.0	0.0	3.6254353702763287	0.0
7	5.129546701957948E-4	0.0	0.0	4.163524819311718	0.0
8	5.129546701957948E-4	0.0	0.0	4.869735161503778	0.0
9	5.129546701957948E-4	0.0	0.0	5.088638567009833	0.0
10	5.129546701957948E-4	0.0	0.0	6.187259231901677	0.0
11	5.129546701957948E-4	0.0	0.0	6.98708380140447	0.0
12	7.694320052936921E-4	0.0	0.0	8.173419714899794	0.0
13	7.694320052936921E-4	0.0	0.0	8.4595201822015	0.0
14	7.694320052936921E-4	0.0	0.0	8.593273112455053	0.0
15	7.694320052936921E-4	0.0	0.0	8.871038066366076	0.0
16	7.694320052936921E-4	0.0	0.0	9.309742548051029	0.0
17	7.694320052936921E-4	0.0	0.0	9.875916265279637	0.0
18	7.694320052936921E-4	0.0	0.0	10.458248054619414	0.0
19	7.694320052936921E-4	0.0	0.0	10.951582208680218	0.0
20	7.694320052936921E-4	0.0	0.0	11.328603891274128	0.0
21	7.694320052936921E-4	0.0	0.0	11.7833382064027	0.0
22	7.694320052936921E-4	0.0	0.0	12.345921240939939	0.0
23	7.694320052936921E-4	0.0	0.0	12.902348819434826	0.0
24	7.694320052936921E-4	0.0	0.0	13.328101195697336	0.0
25	7.694320052936921E-4	0.0	0.0	13.72782112244741	0.0
26	7.694320052936921E-4	0.0	0.0	14.092147176953972	0.0
27	7.694320052936921E-4	0.0	0.0	14.483659828980914	0.0
28	7.694320052936921E-4	0.0	0.0	14.865298103606584	0.0
29	7.694320052936921E-4	0.0	0.0	15.278354851781748	0.0
30	7.694320052936921E-4	0.0	0.0	15.75386383105325	0.0
31	7.694320052936921E-4	0.0	0.0	16.205776895495745	0.0
32	7.694320052936921E-4	0.0	0.0	16.627938589066883	0.0
33	7.694320052936921E-4	0.0	0.0	17.046509599946653	0.0
34	7.694320052936921E-4	0.0	0.0	17.465080610826423	0.0
35	7.694320052936921E-4	0.0	0.0	17.923149131311266	0.0
36	7.694320052936921E-4	0.0	0.0	18.347875598233383	0.0
37	8.976706728426409E-4	0.0	0.0	18.779655191870695	0.0
38	8.976706728426409E-4	0.0	0.0	19.213999558858983	0.0
39	8.976706728426409E-4	0.0	0.0	19.689636776798036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1645992E-6	45.000004	1
GCGAACC	20	7.031888E-4	45.000004	33
CGCATCG	35	1.2112469E-7	45.000004	21
CGTCTCG	25	3.889647E-5	45.000004	42
CGTACTC	20	7.031888E-4	45.000004	42
TAGTACG	20	7.031888E-4	45.000004	1
CGTTTTT	1575	0.0	41.42857	1
TACGGCT	545	0.0	40.871563	7
CGGTCTA	95	0.0	40.26316	31
TAGCACG	40	3.4563163E-7	39.375004	1
CATCGCC	40	3.4563163E-7	39.375004	23
ATAACGC	40	3.4563163E-7	39.375004	11
GCATCGC	40	3.4563163E-7	39.375004	22
CGAACGT	35	6.245704E-6	38.57143	15
TATGCGA	35	6.245704E-6	38.57143	11
GTATGCG	35	6.245704E-6	38.57143	1
ACGTAGG	70	0.0	38.57143	2
TATAACG	35	6.245704E-6	38.57143	1
CTAACGG	60	1.546141E-10	37.500004	2
CGTCACG	30	1.139585E-4	37.500004	37
>>END_MODULE