##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935384.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560627
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91944376564097	33.0	31.0	34.0	30.0	34.0
2	31.968014383895174	33.0	31.0	34.0	30.0	34.0
3	32.04450374313046	33.0	31.0	34.0	30.0	34.0
4	35.76419437522631	37.0	35.0	37.0	33.0	37.0
5	35.79020632256385	37.0	35.0	37.0	35.0	37.0
6	35.7415625719061	37.0	35.0	37.0	35.0	37.0
7	36.02620458879076	37.0	35.0	37.0	35.0	37.0
8	35.88468803678738	37.0	35.0	37.0	35.0	37.0
9	37.72514167173539	39.0	38.0	39.0	35.0	39.0
10	36.956058127774796	39.0	37.0	39.0	32.0	39.0
11	36.925007179461566	39.0	37.0	39.0	33.0	39.0
12	37.01802267817997	39.0	37.0	39.0	33.0	39.0
13	37.032246038810115	39.0	37.0	39.0	33.0	39.0
14	38.108954795256025	40.0	37.0	41.0	33.0	41.0
15	38.25738146753545	40.0	38.0	41.0	34.0	41.0
16	38.155199446334194	40.0	37.0	41.0	33.0	41.0
17	38.1827864159236	40.0	37.0	41.0	34.0	41.0
18	38.089380283147264	40.0	37.0	41.0	33.0	41.0
19	37.993735585335706	40.0	37.0	41.0	34.0	41.0
20	38.07100086153539	40.0	37.0	41.0	34.0	41.0
21	38.010861053784424	40.0	37.0	41.0	34.0	41.0
22	38.10127589288422	40.0	37.0	41.0	34.0	41.0
23	38.118319310343594	40.0	37.0	41.0	34.0	41.0
24	38.09011160718267	40.0	37.0	41.0	34.0	41.0
25	37.84881570099193	40.0	36.0	41.0	33.0	41.0
26	37.91269239619212	40.0	37.0	41.0	34.0	41.0
27	37.93725953263043	40.0	37.0	41.0	34.0	41.0
28	37.8587795450451	40.0	36.0	41.0	33.0	41.0
29	37.85958578520121	40.0	36.0	41.0	33.0	41.0
30	37.695291165070536	40.0	36.0	41.0	33.0	41.0
31	37.68807781287737	40.0	36.0	41.0	33.0	41.0
32	37.656580935274256	40.0	36.0	41.0	33.0	41.0
33	37.58238543630613	40.0	36.0	41.0	33.0	41.0
34	37.531051840171806	40.0	36.0	41.0	33.0	41.0
35	37.49086826000175	40.0	36.0	41.0	33.0	41.0
36	37.42719312484058	40.0	36.0	41.0	33.0	41.0
37	37.352639098723394	40.0	36.0	41.0	33.0	41.0
38	37.28317758509669	40.0	36.0	41.0	32.0	41.0
39	37.24691461524329	40.0	36.0	41.0	32.0	41.0
40	37.14996958762243	40.0	36.0	41.0	32.0	41.0
41	37.0782962647179	40.0	35.0	41.0	32.0	41.0
42	37.09100168204528	40.0	35.0	41.0	32.0	41.0
43	37.0262028050736	40.0	35.0	41.0	32.0	41.0
44	36.880446357382006	39.0	35.0	41.0	31.0	41.0
45	36.84958983423916	39.0	35.0	41.0	31.0	41.0
46	36.774506044125594	39.0	35.0	41.0	31.0	41.0
47	36.735007411344796	39.0	35.0	41.0	31.0	41.0
48	36.64362044639306	39.0	35.0	41.0	31.0	41.0
49	36.56542406983609	39.0	35.0	41.0	31.0	41.0
50	36.45312123747161	39.0	35.0	41.0	31.0	41.0
51	35.35580519668157	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	5.0
10	6.0
11	10.0
12	20.0
13	10.0
14	26.0
15	33.0
16	31.0
17	70.0
18	135.0
19	243.0
20	399.0
21	646.0
22	976.0
23	1490.0
24	2159.0
25	3134.0
26	4295.0
27	5001.0
28	5498.0
29	6329.0
30	7561.0
31	9887.0
32	12875.0
33	18184.0
34	32240.0
35	37783.0
36	41279.0
37	64297.0
38	121689.0
39	184230.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92818576343986	21.448842100005887	24.59888660374902	11.024085532805234
2	33.72598893738618	24.09516487789564	24.860201167621252	17.31864501709693
3	32.50004013363609	23.789079013319018	27.150494000467333	16.56038685257756
4	28.634546677202486	25.87834692228523	26.561332222672114	18.925774177840168
5	25.441871333346416	29.955210862124016	25.600265417113338	19.002652387416234
6	26.602536089057434	34.95425657344366	24.715006590834903	13.728200746664005
7	81.0392649658329	5.7813840574927715	8.791585135928166	4.387765840746165
8	82.92090819742896	5.137818906331661	7.92862277414395	4.012650122095439
9	77.05961361118891	7.485547431714848	9.65151517854117	5.803323778555082
10	37.27380236770616	31.455852108442866	17.709100703319677	13.561244820531298
11	27.995975934088086	26.767708298030595	26.596471450715004	18.639844317166315
12	25.609540746342933	24.081251884051248	31.116945848130754	19.19226152147506
13	23.966202127261084	25.362852663178902	31.58980926712413	19.081135942435882
14	20.78262374091865	28.996284517156685	29.821432075158704	20.39965966676596
15	20.4538846684159	28.0776701799949	32.315068664192054	19.153376487397146
16	23.485133609333836	26.49016190800657	31.107313775469255	18.917390707190343
17	23.710238714867458	25.983586234697935	28.684134014237628	21.622041036196972
18	23.533650716073254	27.207751321288487	29.7042418577771	19.554356104861164
19	23.952467505132645	28.204492470038012	27.98955455231375	19.853485472515594
20	24.723568433200686	27.950134403087972	28.56569519484434	18.760601968867
21	24.87304393116992	26.81444168761048	29.415279677932027	18.89723470328757
22	22.873140251896537	25.656452507638765	29.413317589056536	22.057089651408155
23	22.093655853178674	27.61622255082256	29.363551880305444	20.926569715693322
24	22.146988996248844	27.181887422475192	29.890461929232803	20.780661652043158
25	22.86386492266694	27.886455700492483	28.19628737110414	21.053392005736434
26	21.523044733842646	28.366989103271873	28.501838120532902	21.608128042352583
27	21.919029943259957	27.869510387476883	29.7377757403764	20.473683928886764
28	20.2919231503299	27.8324090705585	30.595922065829868	21.279745713281738
29	21.990913744789317	27.75339040039099	29.31967243818082	20.93602341663887
30	22.019453219341916	27.444628960075057	29.6687458863023	20.867171934280726
31	22.361035055393337	28.88373196438987	27.44427221664315	21.310960763573643
32	23.026183184184852	29.28399809499007	27.492254208234705	20.19756451259037
33	22.75416631735539	28.35807051747419	27.666166631289606	21.221596533880817
34	21.59243133134865	27.853456933041045	28.494346508462847	22.059765227147462
35	22.21494862002722	28.111739177742063	27.98491688769895	21.688395314531768
36	23.516527031341695	28.359140747769906	27.456579865043963	20.66775235584444
37	21.561573024488652	29.145938386841873	28.339519859014995	20.952968729654476
38	21.61722499986622	29.510530174251333	26.927529355525152	21.944715470357295
39	21.76331143523234	28.71213837364237	27.282132326841197	22.242417864284096
40	22.87028630444128	27.428397133923266	29.370330005511686	20.33098655612377
41	21.49093782497097	28.04645512970299	28.525026443606887	21.937580601719148
42	22.56081137726153	27.55432756538661	28.348081701380774	21.536779355971085
43	22.130757170097052	27.36132936872466	27.96333391006855	22.544579551109738
44	21.118140938627644	27.514193929297022	28.92422234391137	22.443442788163967
45	22.260968522743283	27.130694739996468	27.31673643973622	23.291600297524024
46	22.24883924605843	28.094615493010505	27.51615601817251	22.140389242758555
47	21.32701421800948	27.555932910830194	29.50589250963653	21.611160361523794
48	21.09406075697389	27.572699852129844	28.70125769896919	22.631981691927074
49	21.846967770014643	27.18973577797716	28.94170277207484	22.02159367993336
50	20.52683870024098	27.004407565101218	29.302192010017357	23.16656172464045
51	20.542713782960863	26.683338476384478	28.306521091563553	24.467426649091106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	597.0
2	869.0
3	2108.5
4	3348.0
5	2356.0
6	1364.0
7	1350.0
8	1336.0
9	1398.5
10	1461.0
11	1427.0
12	1393.0
13	1450.0
14	1507.0
15	1513.5
16	1520.0
17	1513.5
18	1507.0
19	1564.0
20	1621.0
21	1869.0
22	2117.0
23	2385.5
24	2654.0
25	3456.5
26	5019.5
27	5780.0
28	6858.0
29	7936.0
30	9263.5
31	10591.0
32	12408.0
33	14225.0
34	16566.0
35	18907.0
36	20669.5
37	22432.0
38	24557.0
39	26682.0
40	28578.0
41	30474.0
42	33009.5
43	35545.0
44	37650.0
45	39755.0
46	44193.5
47	48632.0
48	51801.5
49	54971.0
50	52083.0
51	49195.0
52	43635.5
53	38076.0
54	34019.0
55	29962.0
56	27475.0
57	24988.0
58	23090.0
59	21192.0
60	19246.0
61	17300.0
62	15325.5
63	13351.0
64	11316.0
65	9281.0
66	7569.5
67	5858.0
68	4805.5
69	3753.0
70	3191.0
71	2629.0
72	2099.0
73	1569.0
74	1330.0
75	836.5
76	582.0
77	463.5
78	345.0
79	227.5
80	110.0
81	79.0
82	48.0
83	52.5
84	57.0
85	37.5
86	18.0
87	11.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.309456375227114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6888202006941	24.596044492672146
2	8.502012883277597	5.8339888024086335
3	3.6319594072417396	3.7383165851806863
4	2.299422119014837	3.1556769152228736
5	1.704095459599768	2.9233294415180424
6	1.464672737982442	3.015127524467583
7	1.1997216793197447	2.88132590333245
8	1.0697444632488338	2.936188079558129
9	0.9525505382312834	2.94133420229808
>10	7.403721651822975	43.81422268138984
>50	0.052049287229181646	1.2061176690467665
>100	0.026024643614574447	1.5047005447709565
>500	0.0046844358506234005	1.1246859756666845
>1k	5.204928722914889E-4	0.3289411824671376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1842	0.32856070078679767	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	922	0.16445872210935258	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	913	0.16285337666576888	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	748	0.133422043533401	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	736	0.13128158294195605	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	734	0.13092483951004857	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	585	0.10434745383294061	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	574	0.10238536495744943	Illumina Single End Adapter 1 (95% over 22bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	569	0.1014935063776807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.567434319074893E-4	0.0	0.0	0.11273092448276661	0.0
2	3.567434319074893E-4	0.0	0.0	0.6128852160170666	0.0
3	3.567434319074893E-4	0.0	0.0	0.9152252745586638	0.0
4	3.567434319074893E-4	0.0	0.0	1.2609096600770209	0.0
5	3.567434319074893E-4	0.0	0.0	2.1236936501452837	0.0
6	3.567434319074893E-4	0.0	0.0	2.638474422387791	0.0
7	3.567434319074893E-4	0.0	0.0	3.0480158822175887	0.0
8	3.567434319074893E-4	0.0	0.0	3.630399534806565	0.0
9	3.567434319074893E-4	0.0	0.0	3.8408781596319836	0.0
10	3.567434319074893E-4	0.0	0.0	4.466962882629627	0.0
11	3.567434319074893E-4	0.0	0.0	5.256079354008993	0.0
12	3.567434319074893E-4	0.0	0.0	5.978841547053567	0.0
13	3.567434319074893E-4	0.0	0.0	6.2344482160152825	0.0
14	3.567434319074893E-4	0.0	0.0	6.348070999077819	0.0
15	3.567434319074893E-4	0.0	0.0	6.553733587572486	0.0
16	3.567434319074893E-4	0.0	0.0	6.977544784678583	0.0
17	3.567434319074893E-4	0.0	0.0	7.531032219283053	0.0
18	3.567434319074893E-4	0.0	0.0	8.121799342521856	0.0
19	3.567434319074893E-4	0.0	0.0	8.515822463063676	0.0
20	5.35115147861234E-4	0.0	0.0	8.890046323134634	0.0
21	7.134868638149786E-4	0.0	0.0	9.341683507929515	0.0
22	7.134868638149786E-4	0.0	0.0	9.882863294133175	0.0
23	7.134868638149786E-4	0.0	0.0	10.405492421877648	0.0
24	7.134868638149786E-4	0.0	0.0	10.83144407957519	0.0
25	7.134868638149786E-4	0.0	0.0	11.211019091124758	0.0
26	7.134868638149786E-4	0.0	0.0	11.570973213919416	0.0
27	7.134868638149786E-4	0.0	0.0	11.943948471978695	0.0
28	7.134868638149786E-4	0.0	0.0	12.344036230862944	0.0
29	7.134868638149786E-4	1.7837171595374464E-4	0.0	12.734313545369737	0.0
30	7.134868638149786E-4	1.7837171595374464E-4	0.0	13.208603938090745	0.0
31	7.134868638149786E-4	1.7837171595374464E-4	0.0	13.626885612002276	0.0
32	7.134868638149786E-4	1.7837171595374464E-4	0.0	14.040172877867102	0.0
33	7.134868638149786E-4	1.7837171595374464E-4	0.0	14.461843614381754	0.0
34	7.134868638149786E-4	1.7837171595374464E-4	0.0	14.877449712553979	0.0
35	7.134868638149786E-4	1.7837171595374464E-4	0.0	15.31089298232158	0.0
36	7.134868638149786E-4	1.7837171595374464E-4	0.0	15.720434442151378	0.0
37	7.134868638149786E-4	1.7837171595374464E-4	0.0	16.146742843280826	0.0
38	7.134868638149786E-4	1.7837171595374464E-4	0.0	16.575726820149583	0.0
39	7.134868638149786E-4	1.7837171595374464E-4	0.0	17.059649285532092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.0301903E-4	45.000004	1
GTCGTTA	20	7.0301903E-4	45.000004	20
TTGTGCG	50	2.1827873E-11	45.0	1
TAACGCC	25	3.88824E-5	45.0	12
ACTACGG	35	1.2104465E-7	45.0	2
ATAACGC	25	3.88824E-5	45.0	11
ACGCGCA	25	3.88824E-5	45.0	17
ATTAGCG	30	2.1635042E-6	44.999996	1
CACGGGC	95	0.0	42.63158	4
TACGAAT	75	0.0	42.000004	12
CGAATAT	70	0.0	41.785713	14
CGGTCTA	55	6.002665E-11	40.909092	31
TACGGTT	105	0.0	40.714287	33
TAACGGG	145	0.0	40.344826	3
CTACGAA	80	0.0	39.375004	11
GCTACGA	80	0.0	39.375004	10
TATACGG	40	3.453988E-7	39.375004	2
CGTTTTT	1040	0.0	39.375	1
TTTGCGG	105	0.0	38.57143	2
ATGTACG	35	6.242566E-6	38.571426	1
>>END_MODULE