##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935382.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473032
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716985320232034	31.0	31.0	34.0	30.0	34.0
2	31.789048098225912	33.0	31.0	34.0	30.0	34.0
3	31.851999441898222	33.0	31.0	34.0	30.0	34.0
4	35.603439090801466	37.0	35.0	37.0	33.0	37.0
5	35.587624093084614	37.0	35.0	37.0	33.0	37.0
6	35.47352821796411	37.0	35.0	37.0	33.0	37.0
7	35.74643998714674	37.0	35.0	37.0	35.0	37.0
8	35.466970522078846	37.0	35.0	37.0	33.0	37.0
9	37.24778661908708	39.0	37.0	39.0	34.0	39.0
10	36.83022501648937	39.0	37.0	39.0	32.0	39.0
11	36.801284056892555	39.0	35.0	39.0	32.0	39.0
12	36.698445348306244	39.0	35.0	39.0	33.0	39.0
13	36.54150036361177	39.0	35.0	39.0	32.0	39.0
14	37.46181230868102	40.0	36.0	41.0	32.0	41.0
15	37.46367687598302	40.0	36.0	41.0	32.0	41.0
16	37.54343469363595	39.0	36.0	41.0	32.0	41.0
17	37.40730859645859	39.0	36.0	41.0	32.0	41.0
18	37.23535828442896	39.0	36.0	40.0	32.0	41.0
19	37.076115357946186	39.0	35.0	40.0	32.0	41.0
20	36.85918288826126	39.0	35.0	40.0	31.0	41.0
21	36.97062777993878	39.0	35.0	40.0	32.0	41.0
22	37.12659185847892	39.0	35.0	41.0	32.0	41.0
23	37.117740448849126	39.0	35.0	41.0	32.0	41.0
24	37.08204730335368	39.0	35.0	41.0	32.0	41.0
25	36.91944519609667	38.0	35.0	40.0	32.0	41.0
26	36.88424038965651	39.0	35.0	40.0	32.0	41.0
27	36.8627851815522	39.0	35.0	40.0	32.0	41.0
28	36.706315851781696	38.0	35.0	40.0	31.0	41.0
29	36.6062317137107	38.0	35.0	40.0	31.0	41.0
30	36.310644945796476	38.0	35.0	40.0	30.0	41.0
31	36.27572976035448	38.0	35.0	40.0	30.0	41.0
32	36.03152640836138	38.0	35.0	40.0	30.0	41.0
33	35.6510510916809	38.0	35.0	40.0	28.0	41.0
34	35.43591765461956	38.0	35.0	40.0	26.0	41.0
35	35.15917316376059	38.0	34.0	40.0	24.0	41.0
36	35.172516869894636	38.0	35.0	40.0	25.0	41.0
37	35.04660784048436	38.0	35.0	40.0	23.0	41.0
38	34.92708738520861	38.0	34.0	40.0	23.0	41.0
39	34.855261377665784	38.0	34.0	40.0	23.0	41.0
40	34.60861633039625	38.0	34.0	40.0	22.0	41.0
41	34.523727781629994	38.0	34.0	40.0	22.0	41.0
42	34.59521131762756	38.0	34.0	40.0	23.0	41.0
43	34.5785993336603	38.0	34.0	40.0	22.0	41.0
44	34.512589000321334	38.0	34.0	40.0	22.0	41.0
45	34.505479544724245	37.0	34.0	40.0	23.0	41.0
46	34.38295717837271	37.0	34.0	40.0	22.0	41.0
47	34.176962657917436	37.0	33.0	40.0	22.0	41.0
48	34.178495323783594	37.0	34.0	40.0	22.0	41.0
49	34.17563716619595	37.0	34.0	40.0	22.0	41.0
50	34.0624122680918	37.0	33.0	40.0	21.0	41.0
51	32.80863662500634	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	5.0
11	10.0
12	3.0
13	16.0
14	16.0
15	35.0
16	66.0
17	82.0
18	248.0
19	464.0
20	1003.0
21	1657.0
22	2418.0
23	3138.0
24	4104.0
25	5721.0
26	8218.0
27	9733.0
28	9921.0
29	9935.0
30	10754.0
31	12878.0
32	16408.0
33	22717.0
34	37911.0
35	36293.0
36	39584.0
37	53191.0
38	85620.0
39	100835.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.760396759627255	17.41510088112432	19.940088619797393	17.88441373945103
2	33.474056723435204	31.277587985590827	20.516159583284004	14.732195707689966
3	26.10499923895212	30.650991899068142	29.303725752168987	13.940283109810753
4	23.218936562431296	20.849329432258283	39.892649968712476	16.03908403659795
5	28.658526273063977	23.89774898949754	29.048140506350524	18.395584231087962
6	22.268260921037054	34.40930000507365	29.697356627035802	13.62508244685349
7	67.49353109303388	4.812993624109996	23.099282923776826	4.594192359079301
8	66.99694735239899	10.541781528522383	17.160149841871164	5.301121277207462
9	61.69202083579969	5.705110859307616	17.80577212535304	14.79709617953965
10	39.68547582404573	21.0903279270747	24.151008811243212	15.073187437636355
11	32.228686431361936	19.184114393952207	29.50455783118267	19.082641343503187
12	26.423370934735917	17.158035819986807	34.37674406805459	22.041849177222684
13	25.893597050516664	18.39600703546483	38.310727392649966	17.399668521368532
14	19.287067259720274	25.480094369936918	34.58349540834447	20.649342961998343
15	18.13598234368922	20.908099240643338	40.747349016557024	20.20856939911042
16	22.615171912259633	22.594665899981397	31.406543320536457	23.383618867222513
17	22.00612220737709	24.228382012210588	32.994807962252025	20.770687818160294
18	22.716010756143348	22.670136481252854	32.378147778585806	22.235704984017996
19	21.623061441932048	25.67120194828257	29.01664158027364	23.689095029511744
20	23.817627560080503	25.619197009927447	32.751484043362815	17.81169138662923
21	22.377978656835058	28.31605472779854	32.072037409731266	17.23392920563514
22	20.69077779093169	20.751238816824234	35.079022138037175	23.478961254206904
23	21.688807522535473	23.999010637758122	34.225591503323244	20.08659033638316
24	24.01148336687581	22.694870537299803	30.911016590843747	22.382629504980635
25	22.354301611730286	28.34691944731012	27.905300275668456	21.393478665291145
26	21.142121463241388	20.937484144835867	32.12340814152108	25.796986250401666
27	23.55210641140557	21.4951631179286	31.183302609548612	23.76942786111722
28	20.46013800334861	23.594809653469532	32.18408056960206	23.7609717735798
29	26.718699791980242	21.47317729033131	30.693060934566795	21.11506198312165
30	24.548022121124998	23.406450303573543	34.12263863755518	17.922888937746283
31	29.201829897343096	19.886392463934786	29.022560841549833	21.889216797172285
32	31.900801637098546	23.21407431209728	27.486935344754688	17.398188706049485
33	26.329931167447445	24.154391246258182	26.98739197348171	22.528285612812667
34	25.683040470834957	23.284471240846287	28.61518882443471	22.41729946388405
35	21.413350471004076	28.106343756870572	29.70412149706574	20.776184275059613
36	25.287295574083785	30.752676351705592	25.53505893893014	18.424969135280488
37	23.269250283278932	26.716162965719022	29.926093794922963	20.088492956079083
38	24.240431936951413	27.934050973295676	25.177366436097348	22.648150653655566
39	25.962514163946626	24.060740076781276	25.804808131373775	24.171937627898323
40	23.150653655566643	26.61426711089313	28.97901199073213	21.256067242808097
41	19.856373353176952	27.585237362377175	26.983163929713	25.575225354732872
42	20.752507229954844	25.642028446278474	31.796580358199865	21.80888396556681
43	23.382984660657208	24.38756786010249	27.77148269038881	24.457964788851495
44	22.93269799928969	21.361556934837388	29.88021952003247	25.82552554584045
45	22.014578294914507	21.218014848889716	28.502511457998615	28.264895398197158
46	25.948561619509885	24.226690794703106	27.40448849126486	22.420259094522148
47	19.402915658982902	23.481286678279695	34.81709482656564	22.29870283617176
48	20.593532784251384	23.644066363374993	30.103248828831873	25.659152023541747
49	20.880405553958294	21.28798897326185	34.86719714522485	22.96440832755501
50	20.855671497911345	22.015423903668253	32.71491146476349	24.41399313365692
51	20.461829220856096	20.605582708992205	31.212053307175836	27.720534762975866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	264.0
1	466.5
2	669.0
3	7166.0
4	13663.0
5	8812.0
6	3961.0
7	3814.5
8	3668.0
9	3597.5
10	3527.0
11	3382.0
12	3237.0
13	3068.0
14	2899.0
15	2709.0
16	2519.0
17	2345.0
18	2171.0
19	2005.0
20	1839.0
21	1869.5
22	1900.0
23	2001.0
24	2102.0
25	2340.5
26	2712.5
27	2846.0
28	3236.5
29	3627.0
30	4392.0
31	5157.0
32	5594.0
33	6031.0
34	7562.5
35	9094.0
36	9661.0
37	10228.0
38	10909.5
39	11591.0
40	12687.5
41	13784.0
42	14725.0
43	15666.0
44	18215.5
45	20765.0
46	45256.5
47	69748.0
48	53494.0
49	37240.0
50	37592.0
51	37944.0
52	34117.5
53	30291.0
54	28863.5
55	27436.0
56	26534.5
57	25633.0
58	24797.5
59	23962.0
60	22823.0
61	21684.0
62	19815.0
63	17946.0
64	15894.5
65	13843.0
66	11260.5
67	8678.0
68	7171.0
69	5664.0
70	4741.5
71	3819.0
72	3045.5
73	2272.0
74	1922.0
75	1173.5
76	775.0
77	596.5
78	418.0
79	299.5
80	181.0
81	135.0
82	89.0
83	55.0
84	21.0
85	18.5
86	16.0
87	10.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	473032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.01089087301801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43070744093957	29.588567644107705
2	10.004487178657858	8.405948382062062
3	5.400247992285748	6.806076872734536
4	3.779061559357113	6.3504697109027575
5	2.7013120438881897	5.6742262744878005
6	2.0846703723678655	5.254731571185614
7	1.487078326320387	4.373143970067324
8	1.1433481056603643	3.8426457997415544
9	0.8203657721777404	3.1017867237826393
>10	2.091635135485878	11.662873562013685
>50	0.025259324274017742	0.7239279333834677
>100	0.023238578332070468	1.9389638227456973
>500	0.0030311189128787567	1.0494938817291455
>1k	0.0035363053983585497	3.134049777855266
>5k	0.0015155594564393784	5.114399102509393
>10k+	5.051864854797928E-4	2.978694970691316
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	14035	2.9670297146916065	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8509	1.7988212213972838	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	8256	1.7453364677231138	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	7333	1.5502122477971891	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	4051	0.8563902653520269	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTT	2672	0.564866647499535	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	2377	0.5025030019110758	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	1631	0.3447969693382266	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC	1447	0.3058989666661029	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT	1338	0.28285612812663835	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTT	1251	0.26446413773275385	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	971	0.2052715249708265	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA	951	0.2010434812021174	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	868	0.18349709956197466	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	843	0.1782120448510883	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	742	0.15686042381910736	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	570	0.12049924740820918	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	496	0.10485548546398551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1140218843545468E-4	0.0	0.0	0.0839266688088755	0.0
2	2.1140218843545468E-4	0.0	0.0	0.7724635965431514	0.0
3	2.1140218843545468E-4	0.0	0.0	1.1132439243011043	0.0
4	2.1140218843545468E-4	0.0	0.0	2.3106259195995196	0.0
5	2.1140218843545468E-4	0.0	0.0	6.117767931133623	0.0
6	4.2280437687090936E-4	0.0	0.0	7.765013783422686	0.0
7	4.2280437687090936E-4	0.0	0.0	8.912504862250334	0.0
8	4.2280437687090936E-4	0.0	0.0	10.159566371831081	0.0
9	4.2280437687090936E-4	0.0	0.0	10.815758764734733	0.0
10	4.2280437687090936E-4	0.0	0.0	13.373936646992169	0.0
11	4.2280437687090936E-4	0.0	0.0	14.15485633107274	0.0
12	4.2280437687090936E-4	0.0	0.0	16.37563632058719	0.0
13	4.2280437687090936E-4	0.0	0.0	16.788504794601632	0.0
14	4.2280437687090936E-4	0.0	0.0	17.08911870655685	0.0
15	4.2280437687090936E-4	0.0	0.0	17.481058363916183	0.0
16	4.2280437687090936E-4	0.0	0.0	17.829026366080942	0.0
17	4.2280437687090936E-4	0.0	0.0	18.16917248727359	0.0
18	4.2280437687090936E-4	0.0	0.0	18.498325694667592	0.0
19	4.2280437687090936E-4	0.0	0.0	19.041840721135145	0.0
20	4.2280437687090936E-4	0.0	0.0	19.33082751272641	0.0
21	6.34206565306364E-4	0.0	0.0	19.573517225050313	0.0
22	6.34206565306364E-4	0.0	0.0	19.91789139001167	0.0
23	6.34206565306364E-4	0.0	0.0	20.195251061238984	0.0
24	6.34206565306364E-4	0.0	0.0	20.443014426085337	0.0
25	6.34206565306364E-4	0.0	0.0	20.66435251737726	0.0
26	6.34206565306364E-4	0.0	0.0	20.90852204502021	0.0
27	6.34206565306364E-4	0.0	0.0	21.237252448037342	0.0
28	6.34206565306364E-4	0.0	0.0	21.467892235620422	0.0
29	6.34206565306364E-4	0.0	0.0	21.733413384295353	0.0
30	6.34206565306364E-4	0.0	0.0	22.060029765428133	0.0
31	8.456087537418187E-4	0.0	0.0	22.333372795075174	0.0
32	8.456087537418187E-4	0.0	0.0	22.64011737049502	0.0
33	8.456087537418187E-4	0.0	0.0	22.930795379593768	0.0
34	8.456087537418187E-4	0.0	0.0	23.24472762942042	0.0
35	8.456087537418187E-4	0.0	0.0	23.560351096754555	0.0
36	8.456087537418187E-4	0.0	0.0	23.846801062084594	0.0
37	0.0010570109421772734	0.0	0.0	24.17214903008676	0.0
38	0.0010570109421772734	0.0	0.0	24.58374909097059	0.0
39	0.0010570109421772734	0.0	0.0	25.179691860170138	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	20	7.029071E-4	45.000004	1
CATCGAA	35	1.2099008E-7	45.000004	42
TATCGCG	20	7.029071E-4	45.000004	1
CACGACG	25	3.8873135E-5	45.0	26
ACGTCAT	25	3.8873135E-5	45.0	35
CGTTTTT	3945	0.0	43.28897	1
CCGAACC	110	0.0	42.954544	18
CGATGAA	680	0.0	42.683826	19
CCGATGA	665	0.0	41.954887	18
TATGGGC	65	0.0	41.538464	4
TGATACC	1110	0.0	41.351353	4
TACGAAT	60	3.6379788E-12	41.249996	12
CGAATAT	60	3.6379788E-12	41.249996	14
TACGGCT	835	0.0	41.227547	7
TATGCGG	55	6.002665E-11	40.909092	2
GAACCCG	110	0.0	40.909092	20
ATCGAAT	55	6.002665E-11	40.909092	43
GATACCT	1130	0.0	40.420353	5
ACGGCTG	860	0.0	40.2907	8
ATGAATG	780	0.0	40.096153	21
>>END_MODULE