SRR2935381.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733810
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	4519	0.6158269851869012	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	4468	0.6088769572505145	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	4173	0.568675815265532	RNA PCR Primer, Index 36 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3621	0.49345198348346303	No Hit
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1903	0.2593314345675311	RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1468	0.20005178452187897	RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	976	0.13300445619438275	RNA PCR Primer, Index 36 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	946	0.1289162044670964	RNA PCR Primer, Index 36 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT	841	0.11460732342159415	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT	838	0.11419849824886551	RNA PCR Primer, Index 15 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	25	3.8894214E-5	45.000004	21
ATATGCG	25	3.8894214E-5	45.000004	1
AGTAGCG	30	2.1644246E-6	45.000004	1
TATCGTC	25	3.8894214E-5	45.000004	40
GTCGCGT	20	7.0316176E-4	45.0	29
TCGTTCA	20	7.0316176E-4	45.0	16
ACTTGCG	40	6.8084773E-9	45.0	1
CTATACG	20	7.0316176E-4	45.0	1
CAACCGG	35	1.2111195E-7	45.0	2
TTAAGCG	40	6.8084773E-9	45.0	1
ACGTCCC	20	7.0316176E-4	45.0	43
CTCGATC	35	1.2111195E-7	45.0	16
CGCGTAA	20	7.0316176E-4	45.0	31
ATCGCGG	35	1.2111195E-7	45.0	2
TCGCGTA	20	7.0316176E-4	45.0	30
CGTTTTT	2220	0.0	42.77027	1
CGCACGG	80	0.0	42.1875	2
GTTAGCG	55	6.002665E-11	40.909092	1
CGACGGT	50	1.0804797E-9	40.500004	28
TACGGCT	635	0.0	40.03937	7