SRR2935380.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514927
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	9966	1.9354199721513925	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	9414	1.8282203108401773	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	9206	1.7878262355634877	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2767	0.5373577225509635	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTT	1936	0.3759756237291888	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	1795	0.3485931015464328	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	1588	0.3083932285547272	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT	1518	0.2947990686058412	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	1421	0.27596144696238495	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.2540165887591833	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA	1296	0.2516861613393743	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	955	0.1854631821598013	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	608	0.11807498927032375	TruSeq Adapter, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.0296536E-4	45.000004	21
ACAACGA	30	2.1631586E-6	45.000004	13
CTCACGT	20	7.0296536E-4	45.000004	45
CGTGACA	20	7.0296536E-4	45.000004	26
CGATTAT	20	7.0296536E-4	45.000004	10
TAGCACG	30	2.1631586E-6	45.000004	1
CTCGAAT	30	2.1631586E-6	45.000004	43
CGGTCTA	20	7.0296536E-4	45.000004	31
CACAACG	30	2.1631586E-6	45.000004	12
TCGCGCA	20	7.0296536E-4	45.000004	34
TAGTACG	20	7.0296536E-4	45.000004	1
GTGCACG	35	1.2101737E-7	45.0	1
TCTACGG	35	1.2101737E-7	45.0	2
TATAGCG	35	1.2101737E-7	45.0	1
CGAGATA	90	0.0	42.500004	19
TACGGCT	1125	0.0	41.0	7
CGGGTAT	55	6.002665E-11	40.909092	6
CATACGA	55	6.002665E-11	40.909092	18
ACGGCTA	50	1.0786607E-9	40.500004	30
AGGTTCG	50	1.0786607E-9	40.500004	1