##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514927
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.913661548141775	33.0	31.0	34.0	30.0	34.0
2	31.966735090605077	33.0	31.0	34.0	30.0	34.0
3	32.05443101643534	33.0	31.0	34.0	30.0	34.0
4	35.77621876498999	37.0	35.0	37.0	35.0	37.0
5	35.76911872945097	37.0	35.0	37.0	35.0	37.0
6	35.69776880994782	37.0	35.0	37.0	35.0	37.0
7	35.92281624385593	37.0	35.0	37.0	35.0	37.0
8	35.65536085697585	37.0	35.0	37.0	33.0	37.0
9	37.462566538557894	39.0	37.0	39.0	34.0	39.0
10	36.99062585570382	39.0	37.0	39.0	33.0	39.0
11	36.97036473131143	39.0	37.0	39.0	33.0	39.0
12	36.9701025582267	39.0	37.0	39.0	33.0	39.0
13	36.84261264218035	39.0	37.0	39.0	33.0	39.0
14	37.97768615745533	40.0	37.0	41.0	33.0	41.0
15	37.97768615745533	40.0	37.0	41.0	33.0	41.0
16	38.052180211952376	40.0	37.0	41.0	33.0	41.0
17	37.94017987015635	40.0	37.0	41.0	33.0	41.0
18	37.896103719556365	40.0	37.0	41.0	33.0	41.0
19	37.8285601648389	40.0	37.0	41.0	33.0	41.0
20	37.752013392189575	40.0	36.0	41.0	33.0	41.0
21	37.84843288466132	40.0	36.0	41.0	33.0	41.0
22	37.91800973730257	40.0	37.0	41.0	33.0	41.0
23	37.91080871657536	40.0	37.0	41.0	33.0	41.0
24	37.91493745715412	40.0	36.0	41.0	33.0	41.0
25	37.74236736469441	40.0	36.0	41.0	33.0	41.0
26	37.7004254972064	40.0	36.0	41.0	33.0	41.0
27	37.69568113538424	40.0	36.0	41.0	33.0	41.0
28	37.580317210012296	40.0	36.0	41.0	33.0	41.0
29	37.582261174884984	40.0	36.0	41.0	33.0	41.0
30	37.511686122498915	40.0	36.0	41.0	33.0	41.0
31	37.60062300093023	40.0	36.0	41.0	33.0	41.0
32	37.46957529902297	40.0	36.0	41.0	33.0	41.0
33	37.33671763181965	40.0	36.0	41.0	32.0	41.0
34	37.26886529546907	40.0	36.0	41.0	32.0	41.0
35	37.25281059256943	40.0	36.0	41.0	32.0	41.0
36	37.21192324348888	40.0	36.0	41.0	32.0	41.0
37	37.20756922825954	40.0	36.0	41.0	32.0	41.0
38	37.15103888512352	40.0	36.0	41.0	32.0	41.0
39	36.94289287607758	40.0	35.0	41.0	31.0	41.0
40	36.89127390872881	39.0	35.0	41.0	31.0	41.0
41	36.68660023653838	39.0	35.0	41.0	31.0	41.0
42	36.79797136293106	39.0	35.0	41.0	31.0	41.0
43	36.7456357891507	39.0	35.0	41.0	31.0	41.0
44	36.67675029664399	39.0	35.0	41.0	31.0	41.0
45	36.65307703810443	39.0	35.0	41.0	31.0	41.0
46	36.501690530890784	39.0	35.0	41.0	31.0	41.0
47	36.43756882043474	39.0	35.0	41.0	31.0	41.0
48	36.324616887442296	39.0	35.0	41.0	30.0	41.0
49	36.27934445076681	39.0	35.0	40.0	30.0	41.0
50	36.157123242712075	39.0	35.0	40.0	30.0	41.0
51	34.876899055594286	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	3.0
10	3.0
11	11.0
12	4.0
13	12.0
14	15.0
15	20.0
16	27.0
17	44.0
18	99.0
19	248.0
20	502.0
21	1029.0
22	1773.0
23	2408.0
24	2783.0
25	3275.0
26	3936.0
27	4488.0
28	5008.0
29	5781.0
30	7092.0
31	9373.0
32	12666.0
33	18067.0
34	31598.0
35	38542.0
36	38708.0
37	58454.0
38	107815.0
39	161067.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.71031039351209	19.55131504077277	23.000930228945073	10.737444336770066
2	28.796120615155157	32.49353791896715	22.716229679158406	15.994111786719284
3	28.912059379290657	31.13082048523383	24.385398318596614	15.571721816878897
4	26.545121929904624	22.700110889504728	33.05439411800119	17.700373062589456
5	30.72765654160687	27.068885492506706	22.81682646278016	19.386631503106265
6	24.46405024401517	38.75636740741892	23.137066030718916	13.642516317846995
7	73.9205751494872	5.662550225565955	15.899923678502	4.516950946444836
8	73.25038306400712	12.263291689890023	9.472993259238688	5.0133319868641575
9	67.06387507355412	7.82635208485863	10.922130709789931	14.187642131797324
10	39.87652618720712	27.540602842733048	17.681341238661012	14.901529731398819
11	32.61355493108732	23.929217151169	25.099674322768085	18.3575535949756
12	27.892691585409196	20.406581903842678	28.567932930298856	23.132793580449267
13	25.45428769514902	22.523386810169207	33.13440545941464	18.887920035267136
14	20.371042885690592	30.44489801467004	27.038784138334172	22.145274961305194
15	17.778053976583088	25.20396094980454	36.543238167740284	20.47474690587209
16	20.140136368844516	24.98490077234249	30.116696153046934	24.75826670576606
17	19.824363453460396	26.00892942106357	30.08892522629421	24.077781899181826
18	20.6320507567092	26.426658536064334	29.02566771600635	23.915622991220115
19	22.159645930394017	27.247551594692066	26.64746653409124	23.94533594082268
20	25.65548126239253	25.621884267090287	30.264095687349858	18.45853878316732
21	23.517314104717755	29.54981968317839	27.226189343343815	19.706676868760038
22	20.97229316000132	24.281305893845147	29.18704981482812	25.559351131325407
23	22.029530399454682	29.017899624606986	27.855598948977235	21.096971026961103
24	22.81585545135524	25.55371926506087	26.539490063640088	25.0909352199438
25	21.236214065294693	31.370660307189173	25.330969244184125	22.062156383332006
26	19.97020936948344	26.664362132884854	29.082763187791667	24.282665309840034
27	24.145170092071304	26.99314660136291	25.856480627351065	23.005202679214722
28	19.179417665028247	27.515550747970103	30.50723694814993	22.79779463885172
29	22.746525235615923	24.045350117589486	29.78130880687942	23.42681583991517
30	20.377645763380052	26.7919530341194	28.322072837509005	24.50832836499154
31	22.877805980265165	24.368502719803	24.758849312621013	27.99484198731082
32	26.77389222161588	26.881480287497062	25.19366822870038	21.150959262186678
33	22.166637212653445	24.22595824262468	25.51468460577907	28.0927199389428
34	22.214605177044515	23.382926123508767	31.727798309274906	22.674670390171812
35	21.418764213179735	22.331514952604934	29.7071235340155	26.542597300199834
36	19.802418595257194	25.27775781809849	30.776401315137875	24.143422271506445
37	22.289178854478404	24.488325529638182	29.88307080421108	23.339424811672334
38	20.485816436116185	24.517844276955763	25.87726027184436	29.119079015083692
39	24.829733146640205	22.855278515207008	29.24356267975849	23.071425658394297
40	20.962194641182148	22.60903001784719	32.34555577780928	24.08321956316138
41	18.590207932386534	24.966645757553984	32.551992806747364	23.891153503312122
42	20.391434125613923	24.42132574131867	32.84601506621327	22.341225066854136
43	22.72069633171304	23.29262206099117	29.4533011475413	24.53338045975449
44	22.63466471946509	23.800655238509535	28.900989849046567	24.66369019297881
45	20.92937445501984	22.148964804719892	28.021059295783672	28.9006014444766
46	24.15216137433073	25.90172975975235	27.20832273312528	22.737786132791637
47	20.019730952154383	23.63597170084303	34.06113876335869	22.283158583643896
48	21.987388503613133	23.928051937459095	28.185742833450178	25.89881672547759
49	19.727844917823305	22.800319268556514	32.9359307241609	24.535905089459284
50	20.410271747257376	22.38278435584073	31.18791595701915	26.01902793988274
51	20.10382054155249	22.35229459709823	28.698048461238194	28.845836400111086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	460.0
2	701.0
3	1640.0
4	2579.0
5	1794.5
6	1010.0
7	968.5
8	927.0
9	922.0
10	917.0
11	933.5
12	950.0
13	932.0
14	914.0
15	910.0
16	906.0
17	966.5
18	1027.0
19	1130.0
20	1233.0
21	1253.0
22	1273.0
23	1658.0
24	2043.0
25	2524.5
26	3773.5
27	4541.0
28	5459.5
29	6378.0
30	7514.5
31	8651.0
32	9699.5
33	10748.0
34	12142.5
35	13537.0
36	14466.5
37	15396.0
38	16987.0
39	18578.0
40	19887.5
41	21197.0
42	24043.5
43	26890.0
44	29244.5
45	31599.0
46	53636.5
47	75674.0
48	60976.5
49	46279.0
50	45371.5
51	44464.0
52	38842.0
53	33220.0
54	30088.5
55	26957.0
56	25351.0
57	23745.0
58	22763.5
59	21782.0
60	21671.0
61	21560.0
62	19552.0
63	17544.0
64	14159.5
65	10775.0
66	8798.5
67	6822.0
68	5564.5
69	4307.0
70	3417.0
71	2527.0
72	2185.0
73	1843.0
74	1517.5
75	880.5
76	569.0
77	425.0
78	281.0
79	172.5
80	64.0
81	59.5
82	55.0
83	35.0
84	15.0
85	12.5
86	10.0
87	9.0
88	8.0
89	6.0
90	4.0
91	6.5
92	9.0
93	4.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.67691940694199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45898926472051	21.065775148598075
2	8.829240205143954	5.063908195748904
3	3.1901597303612923	2.7445186044852776
4	1.750810695965961	2.008314289001116
5	1.230830410115918	1.7648212237253773
6	0.9206729088782433	1.584123768483374
7	0.8139479139542622	1.6339063110940455
8	0.7273926369489875	1.6687504021591304
9	0.6469288464017486	1.6696733751260011
>10	8.265449748734312	47.83560876359337
>50	0.11468287275794675	2.21260973660295
>100	0.042073006573921296	2.2287615930794704
>500	0.001357193760449074	0.3041608635277406
>1k	0.005428775041796296	2.652212673716939
>5k	0.0020357906406736105	5.562855051058216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	9966	1.9354199721513925	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	9414	1.8282203108401773	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	9206	1.7878262355634877	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2767	0.5373577225509635	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTT	1936	0.3759756237291888	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	1795	0.3485931015464328	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	1588	0.3083932285547272	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT	1518	0.2947990686058412	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	1421	0.27596144696238495	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.2540165887591833	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA	1296	0.2516861613393743	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	955	0.1854631821598013	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	608	0.11807498927032375	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.710114249204256E-4	0.0	0.0	0.12700829437959166	0.0
2	9.710114249204256E-4	0.0	0.0	1.0498575526239642	0.0
3	9.710114249204256E-4	0.0	0.0	1.350288487494344	0.0
4	9.710114249204256E-4	0.0	0.0	1.9983415124862358	0.0
5	9.710114249204256E-4	0.0	0.0	4.719503929683237	0.0
6	9.710114249204256E-4	0.0	0.0	5.664298046130811	0.0
7	9.710114249204256E-4	0.0	0.0	6.535489496569417	0.0
8	9.710114249204256E-4	0.0	0.0	7.508442944339683	0.0
9	9.710114249204256E-4	0.0	0.0	7.815088352329553	0.0
10	9.710114249204256E-4	0.0	0.0	10.460123473812793	0.0
11	9.710114249204256E-4	0.0	0.0	11.375787247512754	0.0
12	0.0011652137099045108	0.0	0.0	14.051312127738495	0.0
13	0.0011652137099045108	0.0	0.0	14.526330916809567	0.0
14	0.0011652137099045108	0.0	0.0	14.753935994810915	0.0
15	0.0011652137099045108	0.0	0.0	15.2751749277082	0.0
16	0.0011652137099045108	0.0	0.0	15.830787665047668	0.0
17	0.0011652137099045108	0.0	0.0	16.424075645674048	0.0
18	0.0011652137099045108	0.0	0.0	17.03736646165379	0.0
19	0.0011652137099045108	0.0	0.0	17.740767137866143	0.0
20	0.0011652137099045108	0.0	0.0	18.174032435665637	0.0
21	0.0011652137099045108	0.0	0.0	18.618755668279192	0.0
22	0.0011652137099045108	0.0	0.0	19.176893035323452	0.0
23	0.0011652137099045108	0.0	0.0	19.667253804908267	0.0
24	0.0011652137099045108	0.0	0.0	20.070611950820215	0.0
25	0.0011652137099045108	0.0	0.0	20.418039838656743	0.0
26	0.0011652137099045108	0.0	0.0	20.787606786981456	0.0
27	0.0011652137099045108	0.0	0.0	21.215628623086378	0.0
28	0.0011652137099045108	0.0	0.0	21.56363911777786	0.0
29	0.0011652137099045108	0.0	0.0	21.927185795268066	0.0
30	0.0011652137099045108	0.0	0.0	22.330155536610043	0.0
31	0.0011652137099045108	0.0	0.0	22.712345633458725	0.0
32	0.0011652137099045108	0.0	0.0	23.09201110060261	0.0
33	0.0011652137099045108	0.0	0.0	23.4508969232532	0.0
34	0.0011652137099045108	0.0	0.0	23.82415371499261	0.0
35	0.0011652137099045108	0.0	0.0	24.218384353510302	0.0
36	0.0011652137099045108	0.0	0.0	24.606012114338537	0.0
37	0.0011652137099045108	0.0	0.0	24.962373307284334	0.0
38	0.0011652137099045108	0.0	0.0	25.304557733426293	0.0
39	0.0013594159948885959	0.0	0.0	25.69043767368967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.0296536E-4	45.000004	21
ACAACGA	30	2.1631586E-6	45.000004	13
CTCACGT	20	7.0296536E-4	45.000004	45
CGTGACA	20	7.0296536E-4	45.000004	26
CGATTAT	20	7.0296536E-4	45.000004	10
TAGCACG	30	2.1631586E-6	45.000004	1
CTCGAAT	30	2.1631586E-6	45.000004	43
CGGTCTA	20	7.0296536E-4	45.000004	31
CACAACG	30	2.1631586E-6	45.000004	12
TCGCGCA	20	7.0296536E-4	45.000004	34
TAGTACG	20	7.0296536E-4	45.000004	1
GTGCACG	35	1.2101737E-7	45.0	1
TCTACGG	35	1.2101737E-7	45.0	2
TATAGCG	35	1.2101737E-7	45.0	1
CGAGATA	90	0.0	42.500004	19
TACGGCT	1125	0.0	41.0	7
CGGGTAT	55	6.002665E-11	40.909092	6
CATACGA	55	6.002665E-11	40.909092	18
ACGGCTA	50	1.0786607E-9	40.500004	30
AGGTTCG	50	1.0786607E-9	40.500004	1
>>END_MODULE