FastQCFastQC Report
Sat 14 Jan 2017
SRR2935379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657360
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC193442.9426798101496896No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC144482.1978824388462943No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG143662.185408299866131No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107441.6344164536935621No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT52130.7930205671169527No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC39350.5986065474017281No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT28960.440550079104296No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT27670.42092612875745405No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC24280.3693562127297067No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT22280.3389314835097968No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT22060.33558476329560666No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA18930.2879700620664476No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC16170.24598393574297187No Hit
CGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG14860.2260557381039309No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT13080.19897772909821101No Hit
CGTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT11580.17615918218327858No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC8810.1340209322137033No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT8760.13326031398320556Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.03108E-445.0000041
CGGCGCC207.03108E-445.00000431
GGCCGAA207.03108E-445.00000442
TTCGTAG207.03108E-445.0000041
CACGATA207.03108E-445.00000417
TCGATCA207.03108E-445.00000417
ACTAGCG207.03108E-445.0000041
GTGCGGC207.03108E-445.00000422
GAGTGCG253.888977E-545.01
ACGTTAG453.8380676E-1045.01
GCGCGAG253.888977E-545.01
CCCCGTA253.888977E-545.040
CGATCGC253.888977E-545.034
TACACGG351.2108649E-745.02
CATACGG302.164079E-644.9999962
CGTTTTT64900.044.3412931
CGGCGAA2350.044.04255331
CCGATGA8450.042.86982318
TACGGCT17500.042.6857157
ACGGTCG950.042.6315840
Produced by FastQC (version 0.11.5)