##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657360
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.727079530242182	31.0	31.0	34.0	30.0	34.0
2	31.790550079104296	33.0	31.0	34.0	30.0	34.0
3	31.859758731897287	33.0	31.0	34.0	30.0	34.0
4	35.62008944870391	37.0	35.0	37.0	33.0	37.0
5	35.61263234757211	37.0	35.0	37.0	33.0	37.0
6	35.52706736035049	37.0	35.0	37.0	33.0	37.0
7	35.82054734087867	37.0	35.0	37.0	35.0	37.0
8	35.522667944505294	37.0	35.0	37.0	33.0	37.0
9	37.38584489473044	39.0	37.0	39.0	34.0	39.0
10	36.865636789582574	39.0	37.0	39.0	32.0	39.0
11	36.75319155409517	39.0	35.0	39.0	32.0	39.0
12	36.942119995132046	39.0	37.0	39.0	33.0	39.0
13	36.87437477181453	39.0	37.0	39.0	32.0	39.0
14	38.057493610806866	40.0	37.0	41.0	33.0	41.0
15	37.98988377753438	40.0	37.0	41.0	33.0	41.0
16	38.07067816721431	40.0	37.0	41.0	33.0	41.0
17	37.923618717293415	40.0	37.0	41.0	33.0	41.0
18	37.73490933430693	39.0	37.0	41.0	33.0	41.0
19	37.50304247292199	39.0	37.0	41.0	33.0	41.0
20	37.251615553121574	39.0	35.0	41.0	32.0	41.0
21	37.39957405379092	39.0	35.0	41.0	33.0	41.0
22	37.53343525617622	39.0	35.0	41.0	33.0	41.0
23	37.479738651576	39.0	35.0	41.0	33.0	41.0
24	37.4251521236461	39.0	35.0	41.0	33.0	41.0
25	37.31396799318486	39.0	35.0	41.0	33.0	41.0
26	37.28612632347572	39.0	35.0	41.0	33.0	41.0
27	37.304621516368506	39.0	35.0	41.0	33.0	41.0
28	37.17437325057807	39.0	35.0	41.0	32.0	41.0
29	37.18329073871242	39.0	35.0	41.0	33.0	41.0
30	37.058165997322625	39.0	35.0	41.0	32.0	41.0
31	36.99545910916393	39.0	35.0	41.0	32.0	41.0
32	36.626417792381645	39.0	35.0	41.0	31.0	41.0
33	36.20752707800901	39.0	35.0	41.0	29.0	41.0
34	35.84519289278325	39.0	35.0	41.0	25.0	41.0
35	35.63366952659121	39.0	35.0	41.0	24.0	41.0
36	35.45352014117074	39.0	35.0	41.0	23.0	41.0
37	35.26971218206158	39.0	35.0	41.0	22.0	41.0
38	35.27584124376293	39.0	35.0	41.0	22.0	41.0
39	35.15407995618839	39.0	35.0	41.0	21.0	41.0
40	34.98042472921991	39.0	35.0	41.0	21.0	41.0
41	34.87956827309237	39.0	35.0	41.0	20.0	41.0
42	34.83648837775344	39.0	35.0	41.0	19.0	41.0
43	34.82552939028842	39.0	35.0	41.0	18.0	41.0
44	34.71821832785688	39.0	35.0	40.0	18.0	41.0
45	34.83863636363636	39.0	35.0	40.0	20.0	41.0
46	34.68109863697213	39.0	35.0	40.0	20.0	41.0
47	34.47834672021419	38.0	34.0	40.0	18.0	41.0
48	34.43540677862967	38.0	34.0	40.0	18.0	41.0
49	34.42835280516003	38.0	34.0	40.0	18.0	41.0
50	34.30043050991846	38.0	34.0	40.0	18.0	41.0
51	33.04463307776561	37.0	32.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	2.0
13	11.0
14	16.0
15	21.0
16	44.0
17	93.0
18	227.0
19	577.0
20	1199.0
21	2302.0
22	3294.0
23	4211.0
24	5761.0
25	8851.0
26	13281.0
27	15190.0
28	13948.0
29	12704.0
30	12988.0
31	14796.0
32	18835.0
33	24572.0
34	35187.0
35	42838.0
36	49648.0
37	70699.0
38	128901.0
39	177094.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87489351344773	17.32079834489473	20.459869782158936	18.3444383594986
2	34.11585736886942	32.19149324571011	20.396738469027625	13.295910916392845
3	23.590726542533773	31.663624193744678	32.115127175368144	12.630522088353413
4	21.33397225264695	20.152123646099547	43.72094438359498	14.792959717658514
5	28.336375806255326	23.732049409760254	30.80853717901911	17.123037604965315
6	20.512048192771086	35.31154922721188	31.404101253498844	12.772301326518193
7	66.71778021175612	3.6966046002190582	25.549470609711573	4.036144578313253
8	66.34264330047462	10.78024218084459	18.06103200681514	4.816082511865645
9	61.33092977972496	5.651849823536571	18.24647073141049	14.770749665327978
10	33.00337714494341	29.389984179140804	24.728611415358404	12.878027260557381
11	22.61835219666545	21.14199221126932	38.19672629913594	18.042929292929294
12	18.83047340878666	19.518072289156628	39.84711573566995	21.804338566386757
13	19.639162711451867	20.98271875380309	44.45113788487283	14.926980649872215
14	17.17293416088597	28.66846172569064	35.61959961056347	18.539004502859925
15	14.269350127783861	24.489929414628207	43.857095046854084	17.383625410733845
16	17.129578921747594	26.9934282584885	33.53398442253864	22.343008397225265
17	17.354417670682732	26.593343069246682	37.00924911768285	19.042990142387733
18	17.22602531337471	25.64819885603018	35.819794328830476	21.305981501764634
19	16.30704636728733	27.056559571619815	33.90638310819033	22.730010952902518
20	18.9856395278082	26.468601679445054	38.394182791773154	16.15157600097359
21	17.974017281246198	29.930023122794207	36.098180601192645	15.997778994766945
22	17.10797736400146	21.78121577217963	38.143787270293295	22.967019593525617
23	17.54350736278447	27.768954606304003	36.642631130582934	18.044906900328588
24	18.99644030668127	25.18513447730315	34.45524522331751	21.363179992698065
25	16.492332968236585	33.05662042107825	32.41374589266155	18.037300718023612
26	17.380278690519653	24.436838262139467	36.254867956675184	21.928015090665692
27	20.80047462577583	26.572045758792743	32.21172569064135	20.41575392479007
28	15.314439576487771	25.616709261287575	37.92290373615675	21.145947426067906
29	18.03638797614701	23.039582572715105	38.43829864914202	20.485730801995864
30	16.33473287087745	30.73369234513813	35.28492758914445	17.646647194839964
31	19.322593403918706	23.55680296945357	32.45360228793964	24.667001338688085
32	20.474625775830596	27.25660216624072	34.68419131069733	17.58458074723135
33	19.538152610441767	27.278355847632955	30.853109407326272	22.330382134599002
34	23.730832420591458	23.956279664110987	33.6152184495558	18.697669465741754
35	23.285718632104174	26.654801022270902	30.26347815504442	19.796002190580502
36	23.373189728611415	25.988955823293175	31.21972739442619	19.418127053669224
37	20.056285749056833	27.047280029207737	35.0345320676646	17.86190215407083
38	19.80132651819399	27.62504563709383	28.81404405500791	23.759583789704273
39	24.64129244249726	24.37522818546915	32.47702932943896	18.506450042594622
40	22.63219544846051	27.27531337471096	31.76600340756967	18.326487769258854
41	23.77038456857734	27.02370086406231	29.069763904101254	20.136150663259098
42	22.621242545941342	24.74321528538396	33.910186199342824	18.725355969331872
43	24.78444079347694	24.47988925398564	29.362297675550685	21.373372276986736
44	21.119630035292687	24.680996714129243	31.27677376171352	22.92259948886455
45	19.5281124497992	22.967628088110015	30.976177437020812	26.528082025069978
46	25.211908239016672	25.969940367530732	29.022453450164292	19.795697943288303
47	18.427649993915054	24.16910064500426	37.218267007423634	20.184982353657052
48	20.976633807959107	25.50003042472922	30.292229524157232	23.231106243154436
49	19.403066812705365	22.11117196056955	36.8236582694414	21.66210295728368
50	20.65489229645856	22.58108190337106	34.03599245466715	22.728033345503228
51	19.89427406596081	22.305433856638675	31.163593769015456	26.636698308385053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	264.0
1	404.0
2	544.0
3	9226.0
4	17908.0
5	12186.0
6	6464.0
7	6309.0
8	6154.0
9	6192.5
10	6231.0
11	6222.0
12	6213.0
13	5984.5
14	5756.0
15	5376.5
16	4997.0
17	4792.0
18	4587.0
19	4417.0
20	4247.0
21	4204.5
22	4162.0
23	4245.5
24	4329.0
25	4791.0
26	6114.5
27	6976.0
28	8071.5
29	9167.0
30	10563.5
31	11960.0
32	13434.0
33	14908.0
34	17033.0
35	19158.0
36	21435.5
37	23713.0
38	25962.5
39	28212.0
40	29926.5
41	31641.0
42	33936.0
43	36231.0
44	40371.0
45	44511.0
46	81603.0
47	118695.0
48	90532.0
49	62369.0
50	58182.5
51	53996.0
52	45120.5
53	36245.0
54	30974.0
55	25703.0
56	22156.0
57	18609.0
58	16234.0
59	13859.0
60	11759.0
61	9659.0
62	7896.0
63	6133.0
64	5056.0
65	3979.0
66	3065.0
67	2151.0
68	1634.5
69	1118.0
70	812.0
71	506.0
72	433.5
73	361.0
74	296.5
75	168.5
76	105.0
77	66.0
78	27.0
79	21.0
80	15.0
81	9.5
82	4.0
83	4.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	657360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.538522062660334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.57677864084344	29.126173510749414
2	8.645576144381915	6.663754539693446
3	3.5675252874159464	4.124614559945343
4	2.2720111827370495	3.5023981237009107
5	1.6663299091751467	3.210894598420859
6	1.2883328615792191	2.979026664601264
7	1.05181175922713	2.8374689482118227
8	0.858554528806155	2.646993812031436
9	0.775601037741196	2.69014659343302
>10	4.227941552466225	25.774409092606554
>50	0.043855781976845636	1.1261489546972137
>100	0.01619898245599963	1.1249308337581279
>500	0.003160777064585294	0.7881215478522022
>1k	0.004346068463804779	3.642473660687864
>5k	3.9509713307316177E-4	0.7937553378967408
>10k+	0.001580388532292647	8.96868922171376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	19344	2.9426798101496896	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	14448	2.1978824388462943	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	14366	2.185408299866131	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10744	1.6344164536935621	No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	5213	0.7930205671169527	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	3935	0.5986065474017281	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	2896	0.440550079104296	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	2767	0.42092612875745405	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC	2428	0.3693562127297067	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	2228	0.3389314835097968	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT	2206	0.33558476329560666	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA	1893	0.2879700620664476	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	1617	0.24598393574297187	No Hit
CGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	1486	0.2260557381039309	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	1308	0.19897772909821101	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	1158	0.17615918218327858	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	881	0.1340209322137033	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	876	0.13326031398320556	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14801630765486187	0.0
2	0.0	0.0	0.0	1.0446330777656079	0.0
3	0.0	0.0	0.0	1.4856395278082024	0.0
4	0.0	0.0	0.0	2.7862967019593525	0.0
5	0.0	0.0	0.0	6.920865279299014	0.0
6	0.0	0.0	0.0	8.545393695996106	0.0
7	0.0	0.0	0.0	9.895947426067908	0.0
8	0.0	0.0	0.0	11.315108920530607	0.0
9	0.0	0.0	0.0	11.891049044663502	0.0
10	0.0	0.0	0.0	14.971248630887185	0.0
11	0.0	0.0	0.0	15.819794328830474	0.0
12	0.0	0.0	0.0	18.8513143483023	0.0
13	0.0	0.0	0.0	19.340543994158452	0.0
14	0.0	0.0	0.0	19.64296580260436	0.0
15	0.0	0.0	0.0	20.167031763417306	0.0
16	0.0	0.0	0.0	20.63952780820251	0.0
17	0.0	0.0	0.0	21.193866374589266	0.0
18	1.5212364609954972E-4	0.0	0.0	21.784410368747718	0.0
19	1.5212364609954972E-4	0.0	0.0	22.522818546914934	0.0
20	3.0424729219909945E-4	0.0	0.0	22.945570159425582	0.0
21	3.0424729219909945E-4	0.0	0.0	23.339570402823416	0.0
22	3.0424729219909945E-4	0.0	0.0	23.852987708409394	0.0
23	3.0424729219909945E-4	0.0	0.0	24.32806985517829	0.0
24	3.0424729219909945E-4	0.0	0.0	24.705336497505172	0.0
25	3.0424729219909945E-4	0.0	0.0	25.039400024339784	0.0
26	3.0424729219909945E-4	0.0	0.0	25.39247900693684	0.0
27	3.0424729219909945E-4	0.0	0.0	25.792564196178652	0.0
28	3.0424729219909945E-4	0.0	0.0	26.142448582207617	0.0
29	3.0424729219909945E-4	0.0	0.0	26.525495923086286	0.0
30	3.0424729219909945E-4	0.0	0.0	26.98277960326153	0.0
31	7.606182304977486E-4	0.0	0.0	27.38499452354874	0.0
32	9.127418765972983E-4	0.0	0.0	27.74765729585007	0.0
33	0.001064865522696848	0.0	0.0	28.108342460752098	0.0
34	0.001064865522696848	0.0	0.0	28.459748083242058	0.0
35	0.001064865522696848	0.0	0.0	28.839448703906534	0.0
36	0.001064865522696848	0.0	0.0	29.193896799318487	0.0
37	0.0012169891687963978	0.0	0.0	29.55655957161981	0.0
38	0.0013691128148959474	0.0	0.0	29.926980649872217	0.0
39	0.0013691128148959474	0.0	0.0	30.32158938785445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.03108E-4	45.000004	1
CGGCGCC	20	7.03108E-4	45.000004	31
GGCCGAA	20	7.03108E-4	45.000004	42
TTCGTAG	20	7.03108E-4	45.000004	1
CACGATA	20	7.03108E-4	45.000004	17
TCGATCA	20	7.03108E-4	45.000004	17
ACTAGCG	20	7.03108E-4	45.000004	1
GTGCGGC	20	7.03108E-4	45.000004	22
GAGTGCG	25	3.888977E-5	45.0	1
ACGTTAG	45	3.8380676E-10	45.0	1
GCGCGAG	25	3.888977E-5	45.0	1
CCCCGTA	25	3.888977E-5	45.0	40
CGATCGC	25	3.888977E-5	45.0	34
TACACGG	35	1.2108649E-7	45.0	2
CATACGG	30	2.164079E-6	44.999996	2
CGTTTTT	6490	0.0	44.341293	1
CGGCGAA	235	0.0	44.042553	31
CCGATGA	845	0.0	42.869823	18
TACGGCT	1750	0.0	42.685715	7
ACGGTCG	95	0.0	42.63158	40
>>END_MODULE