FastQCFastQC Report
Sat 14 Jan 2017
SRR2935378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677537
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGC76931.135436145922658No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC69681.0284309196398131No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCG67861.0015689180074299No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC28300.4176893660420022No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.26153553237682964No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT14630.2159291669680032No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT14510.21415804598125268No Hit
GAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT13820.2039741003074371No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTT12040.17770247233730407No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTA11340.16737093324792593No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC10830.15984366905423616No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTC10430.15393993243173434No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT10270.15157843778273364No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6860.10124908307590581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCG207.031234E-445.01
GTCGTAG207.031234E-445.01
ACGTTAG253.8891056E-544.9999961
TAACGCG253.8891056E-544.9999961
TAGCCCG253.8891056E-544.9999961
GCGATAT502.1827873E-1144.9999969
CGTACCT253.8891056E-544.99999633
TACGGCT10000.040.7257
CGAATAT950.040.2631614
ACGGCTG10200.040.147068
CGCATCG403.455425E-739.37521
ATCCGCC403.455425E-739.37518
TTGCGAG403.455425E-739.3751
TGTAGCG403.455425E-739.3751
AACCCGC356.2444924E-638.57142621
GCTATCG356.2444924E-638.5714261
CGATAGT356.2444924E-638.57142610
TAGTACG356.2444924E-638.5714261
CCACCGA5900.038.5169515
CTACGAA1000.038.24999611
Produced by FastQC (version 0.11.5)