##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677537
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.732088432070867	31.0	31.0	34.0	30.0	34.0
2	31.79157300634504	33.0	31.0	34.0	30.0	34.0
3	31.892580036219424	33.0	31.0	34.0	30.0	34.0
4	35.64612117124231	37.0	35.0	37.0	33.0	37.0
5	35.656973715088625	37.0	35.0	37.0	33.0	37.0
6	35.595942361819354	37.0	35.0	37.0	33.0	37.0
7	35.879789590826775	37.0	35.0	37.0	35.0	37.0
8	35.66578061419524	37.0	35.0	37.0	33.0	37.0
9	37.47686399414349	39.0	37.0	39.0	35.0	39.0
10	36.83174645812701	39.0	37.0	39.0	32.0	39.0
11	36.82890085707496	39.0	37.0	39.0	33.0	39.0
12	36.85785278147171	39.0	35.0	39.0	33.0	39.0
13	36.76344465320713	39.0	35.0	39.0	33.0	39.0
14	37.72661566822181	40.0	37.0	41.0	33.0	41.0
15	37.79114498543991	40.0	37.0	41.0	33.0	41.0
16	37.80877059112639	40.0	36.0	41.0	33.0	41.0
17	37.75345995864433	39.0	36.0	41.0	33.0	41.0
18	37.72496409790166	39.0	36.0	41.0	33.0	41.0
19	37.640508193648465	39.0	36.0	41.0	33.0	41.0
20	37.65871974519473	39.0	36.0	41.0	33.0	41.0
21	37.62088122124696	39.0	36.0	41.0	33.0	41.0
22	37.753173627418136	39.0	36.0	41.0	33.0	41.0
23	37.74169676342399	39.0	36.0	41.0	33.0	41.0
24	37.71229320317562	39.0	36.0	41.0	33.0	41.0
25	37.516274387967	39.0	36.0	41.0	33.0	41.0
26	37.567641324385235	39.0	36.0	41.0	33.0	41.0
27	37.555343841000564	39.0	36.0	41.0	33.0	41.0
28	37.42969461446386	39.0	36.0	41.0	33.0	41.0
29	37.41880517226365	39.0	36.0	41.0	33.0	41.0
30	37.28400220209376	39.0	36.0	41.0	32.0	41.0
31	37.31911024785362	39.0	36.0	41.0	32.0	41.0
32	37.24906831656426	39.0	35.0	41.0	32.0	41.0
33	37.15165371042467	39.0	35.0	41.0	32.0	41.0
34	37.10816383459501	39.0	35.0	41.0	32.0	41.0
35	37.099557662533556	40.0	35.0	41.0	32.0	41.0
36	37.03443206791658	39.0	35.0	41.0	31.0	41.0
37	37.0258170402502	39.0	35.0	41.0	31.0	41.0
38	36.85381905342439	39.0	35.0	41.0	31.0	41.0
39	36.817189319550074	39.0	35.0	41.0	31.0	41.0
40	36.68345492570885	39.0	35.0	41.0	31.0	41.0
41	36.55771123938619	39.0	35.0	41.0	30.0	41.0
42	36.686868761410814	39.0	35.0	41.0	31.0	41.0
43	36.647130710204756	39.0	35.0	41.0	31.0	41.0
44	36.55647735843208	39.0	35.0	41.0	31.0	41.0
45	36.47447445674553	39.0	35.0	41.0	31.0	41.0
46	36.349031860990614	39.0	35.0	40.0	31.0	41.0
47	36.261253628953106	38.0	35.0	40.0	30.0	41.0
48	36.19946216959369	38.0	35.0	40.0	30.0	41.0
49	36.14395228600062	38.0	35.0	40.0	30.0	41.0
50	36.01170563378827	38.0	35.0	40.0	30.0	41.0
51	34.88571546646161	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	8.0
10	4.0
11	7.0
12	14.0
13	18.0
14	17.0
15	30.0
16	48.0
17	94.0
18	194.0
19	375.0
20	708.0
21	1245.0
22	1927.0
23	2495.0
24	3207.0
25	4227.0
26	5262.0
27	6248.0
28	7235.0
29	8793.0
30	10765.0
31	13988.0
32	18831.0
33	26341.0
34	44054.0
35	51748.0
36	55909.0
37	82518.0
38	145151.0
39	186007.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.44646713020838	19.847772151188792	23.57686738879205	10.12889332981077
2	31.36906176341661	28.11846437906712	23.380715739509426	17.13175811800684
3	31.08435406479646	27.938105225249693	24.85664989513488	16.12089081481897
4	27.386696224708025	23.71014424304503	29.73107003750349	19.17208949474346
5	28.090274036694673	28.02326662602928	24.114845388517526	19.771613948758517
6	24.53194438089728	37.06572482388415	24.06112138525276	14.341209409965803
7	77.48993781889403	5.621833198777336	12.38899130232002	4.499237680008619
8	78.20074770824323	8.443228930670944	8.431569050841505	4.924454310244311
9	72.42910719266992	7.268680529624212	9.966245385860846	10.33596689184502
10	39.094839101038026	29.586576083667754	16.918485632518962	14.400099182775259
11	29.97784622832406	24.322804511045153	25.661624383612995	20.037724877017787
12	25.681697088129507	22.104180288308978	30.243219189505517	21.970903434055998
13	25.380016146719665	23.459530623419827	32.436900125011626	18.723553104848886
14	21.012136606561707	29.222315534059394	28.570543010935197	21.195004848443702
15	18.739050413482953	25.591222324389662	34.79824718059678	20.871480081530603
16	22.482167025564657	25.826338635380797	28.88166993094104	22.809824408113506
17	22.40763235070557	25.753279894677338	28.30959785222062	23.529489902396474
18	23.392080432507743	24.53135400723503	29.446657525714464	22.629908034542762
19	23.63590475501707	26.727839217636824	26.461285509130867	23.17497051821524
20	25.750770806612778	25.931129960430205	28.65260494998797	19.66549428296905
21	24.130195103736032	28.079352123943046	28.342953964137752	19.44749880818317
22	23.354444111539298	22.99918676028025	29.105126362102734	24.54124276607772
23	22.010901249673452	27.719371783386	28.532316316304495	21.737410650636054
24	22.385640931786753	25.43329736973774	28.186504943641456	23.994556754834054
25	22.41515961489926	29.170362651781378	25.93039199335239	22.484085739966968
26	21.011989013146145	25.748409311963776	27.170176684077767	26.069424990812312
27	22.462389507879273	26.42674864988923	26.933584438931014	24.177277403300483
28	19.736043935607945	26.64592487199961	29.66037279144903	23.95765840094342
29	21.968541939407	25.89246048555282	26.94922934098064	25.18976823405954
30	21.550262199702747	26.40062461533466	28.52906926116212	23.520043923800472
31	25.378392619148478	25.879767451814438	24.635407365206625	24.106432563830463
32	24.366492162051667	28.689946084125296	24.726620096024277	22.21694165779876
33	22.436117879909144	26.607402990537786	25.332491066908524	25.62398806264455
34	21.272934171860726	27.55790458676057	27.338728364650194	23.8304328767285
35	19.561588518413018	27.261832195142112	26.218789527361608	26.957789759083266
36	22.92760395373242	27.3564395745177	25.890394177734944	23.82556229401494
37	20.881959214035543	28.208201175729148	26.79366588097772	24.11617372925759
38	21.89607357236579	29.479275670553783	24.96867329754685	23.655977459533574
39	21.75630260782806	28.05677033136198	26.432652386511734	23.75427467429823
40	21.834231931245082	25.911647629575953	28.07256282682717	24.1815576123518
41	19.469195040270865	26.963988682536893	27.700037045947308	25.866779231244934
42	21.187772771081136	24.717912084506086	28.274027839070044	25.82028730534273
43	21.995551534454947	24.3927637900218	28.095439806239362	25.516244869283895
44	22.022708722918452	25.005424058021923	28.36391222914763	24.60795498991199
45	21.8396928876209	24.205172558841806	26.518699347784697	27.436435205752602
46	23.39370396007893	26.507187061370818	26.111931894494322	23.987177084055926
47	19.873010625249986	26.072229265707996	30.387417956510127	23.66734215253189
48	21.02512482713121	26.64592487199961	27.305962626395313	25.022987674473868
49	21.58937445482682	23.97477923714867	30.610874387671817	23.824971920352688
50	20.865281158076975	24.61902449607918	29.53329486064968	24.98239948519417
51	20.152552554325446	24.303026993359772	27.272311327647053	28.272109124667733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	542.0
2	786.0
3	2000.5
4	3215.0
5	2184.5
6	1154.0
7	1130.0
8	1106.0
9	1126.5
10	1147.0
11	1152.5
12	1158.0
13	1167.5
14	1177.0
15	1203.0
16	1229.0
17	1244.5
18	1260.0
19	1233.5
20	1207.0
21	1567.0
22	1927.0
23	2134.0
24	2341.0
25	2896.5
26	4384.5
27	5317.0
28	6079.5
29	6842.0
30	8216.5
31	9591.0
32	11534.0
33	13477.0
34	15729.5
35	17982.0
36	19116.5
37	20251.0
38	22454.5
39	24658.0
40	27094.5
41	29531.0
42	32083.5
43	34636.0
44	38361.5
45	42087.0
46	61491.0
47	80895.0
48	73278.0
49	65661.0
50	64258.0
51	62855.0
52	55801.0
53	48747.0
54	44462.5
55	40178.0
56	37753.0
57	35328.0
58	32848.0
59	30368.0
60	27946.0
61	25524.0
62	22932.0
63	20340.0
64	17686.5
65	15033.0
66	12594.5
67	10156.0
68	8298.5
69	6441.0
70	5302.5
71	4164.0
72	3400.0
73	2636.0
74	2216.0
75	1346.5
76	897.0
77	629.5
78	362.0
79	258.5
80	155.0
81	115.5
82	76.0
83	71.0
84	66.0
85	42.0
86	18.0
87	11.5
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	677537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.04657947741991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74864635560184	22.585966309539067
2	9.419594509142739	5.848923791463364
3	3.490137215994433	3.2507046739041665
4	1.9656168389052442	2.441027176449062
5	1.3871706090363964	2.153345128109473
6	1.080968176092117	2.013621863496333
7	0.880877786519806	1.9143769546356617
8	0.7653647648594051	1.9009566401139457
9	0.6672639910079518	1.86446380763239
>10	7.4437003372889246	45.77513220219948
>50	0.09583083077893843	1.969713728440934
>100	0.04672348963201487	2.5943618033749924
>500	0.0019070812094699947	0.38292999288703744
>1k	0.004767703023674987	2.129872310650012
>5k	0.0014303109071024962	3.1746036171040943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGC	7693	1.135436145922658	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	6968	1.0284309196398131	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCG	6786	1.0015689180074299	No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	2830	0.4176893660420022	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1772	0.26153553237682964	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1463	0.2159291669680032	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	1451	0.21415804598125268	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	1382	0.2039741003074371	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTT	1204	0.17770247233730407	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTA	1134	0.16737093324792593	No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	1083	0.15984366905423616	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTC	1043	0.15393993243173434	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	1027	0.15157843778273364	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	686	0.10124908307590581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4278024668763477E-4	0.0	0.0	0.16412387810554996	0.0
2	4.4278024668763477E-4	0.0	0.0	0.962604256298918	0.0
3	4.4278024668763477E-4	0.0	0.0	1.2685654067600736	0.0
4	4.4278024668763477E-4	0.0	0.0	1.8649903990483176	0.0
5	4.4278024668763477E-4	0.0	0.0	3.829015979939103	0.0
6	4.4278024668763477E-4	0.0	0.0	4.6184931597831556	0.0
7	4.4278024668763477E-4	0.0	0.0	5.336239939663812	0.0
8	4.4278024668763477E-4	0.0	0.0	6.239806829737712	0.0
9	4.4278024668763477E-4	0.0	0.0	6.51344502219067	0.0
10	4.4278024668763477E-4	0.0	0.0	8.198961827914934	0.0
11	4.4278024668763477E-4	0.0	0.0	9.136770390399343	0.0
12	4.4278024668763477E-4	0.0	0.0	11.087807750720625	0.0
13	4.4278024668763477E-4	0.0	0.0	11.49870781964675	0.0
14	4.4278024668763477E-4	0.0	0.0	11.689250919137995	0.0
15	5.903736622501797E-4	0.0	0.0	12.117567011100501	0.0
16	5.903736622501797E-4	0.0	0.0	12.669271198473293	0.0
17	5.903736622501797E-4	0.0	0.0	13.338459744633873	0.0
18	5.903736622501797E-4	0.0	0.0	14.025654687493082	0.0
19	5.903736622501797E-4	0.0	0.0	14.6611919349054	0.0
20	7.379670778127247E-4	0.0	0.0	15.119764677058226	0.0
21	7.379670778127247E-4	0.0	0.0	15.671321271015458	0.0
22	7.379670778127247E-4	0.0	0.0	16.26641792256364	0.0
23	7.379670778127247E-4	0.0	0.0	16.835242946141687	0.0
24	7.379670778127247E-4	0.0	0.0	17.324957898978212	0.0
25	8.855604933752695E-4	0.0	0.0	17.7348248139954	0.0
26	8.855604933752695E-4	0.0	0.0	18.15148102612846	0.0
27	8.855604933752695E-4	0.0	0.0	18.59632758063397	0.0
28	8.855604933752695E-4	0.0	0.0	19.020953837207415	0.0
29	8.855604933752695E-4	0.0	0.0	19.434953367860352	0.0
30	8.855604933752695E-4	0.0	0.0	19.939722849084255	0.0
31	8.855604933752695E-4	0.0	0.0	20.371581183020265	0.0
32	8.855604933752695E-4	0.0	0.0	20.78602349391989	0.0
33	8.855604933752695E-4	0.0	0.0	21.215520333206896	0.0
34	8.855604933752695E-4	0.0	0.0	21.65018294203859	0.0
35	0.0010331539089378145	0.0	0.0	22.128385608461237	0.0
36	0.0010331539089378145	0.0	0.0	22.57042788807106	0.0
37	0.0010331539089378145	0.0	0.0	22.999924727358064	0.0
38	0.0010331539089378145	0.0	0.0	23.444918875279136	0.0
39	0.0010331539089378145	0.0	0.0	24.025403778686623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTCG	20	7.031234E-4	45.0	1
GTCGTAG	20	7.031234E-4	45.0	1
ACGTTAG	25	3.8891056E-5	44.999996	1
TAACGCG	25	3.8891056E-5	44.999996	1
TAGCCCG	25	3.8891056E-5	44.999996	1
GCGATAT	50	2.1827873E-11	44.999996	9
CGTACCT	25	3.8891056E-5	44.999996	33
TACGGCT	1000	0.0	40.725	7
CGAATAT	95	0.0	40.26316	14
ACGGCTG	1020	0.0	40.14706	8
CGCATCG	40	3.455425E-7	39.375	21
ATCCGCC	40	3.455425E-7	39.375	18
TTGCGAG	40	3.455425E-7	39.375	1
TGTAGCG	40	3.455425E-7	39.375	1
AACCCGC	35	6.2444924E-6	38.571426	21
GCTATCG	35	6.2444924E-6	38.571426	1
CGATAGT	35	6.2444924E-6	38.571426	10
TAGTACG	35	6.2444924E-6	38.571426	1
CCACCGA	590	0.0	38.51695	15
CTACGAA	100	0.0	38.249996	11
>>END_MODULE