FastQCFastQC Report
Sat 14 Jan 2017
SRR2935377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697301
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127791.8326375553742213No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC75181.078157065600078No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC54810.7860307098369284No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG51960.7451588338465025No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT29940.4293698130362641No Hit
GAACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT25330.36325776099561025No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC24890.3569477169830532No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC22960.32926956938251917No Hit
CGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTG20760.2977193493197342No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCT17090.24508784585136117No Hit
CGTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT15070.2161190074300768No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC12480.17897579381070727No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT11680.1675029865151491No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCT11350.16277045350573138No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT10690.1533053874868959Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTC10250.1469953434743389No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATCTGGT9390.1346620756316139No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC8490.12175516742411097No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTA7640.10956530967258042No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTC7530.10798779866944117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA207.0313766E-445.05
GTCGATG207.0313766E-445.01
GTACGAG207.0313766E-445.01
AGTTGCG302.1642682E-644.9999961
TTGCGCG302.1642682E-644.9999961
CGTTTTT78950.044.1450271
GCGTAAG603.6379788E-1241.2499961
CGTAAGG1100.040.9090922
CGAACGG501.0804797E-940.4999962
TAGTGCG501.0804797E-940.4999961
TATAGCG451.9264917E-840.01
TCGACGG403.455607E-739.3752
GTTTTTT89100.039.1414152
CTAGCGG750.039.0000042
GCGATCG356.2447543E-638.571439
TTACCGG356.2447543E-638.571432
TGCGTAG356.2447543E-638.571431
CGTTCTG3800.038.4868431
CGTTTTC1950.038.0769231
GGCGATC1250.037.88
Produced by FastQC (version 0.11.5)