##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	697301
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825081277669184	33.0	31.0	34.0	30.0	34.0
2	31.89249262513606	33.0	31.0	34.0	30.0	34.0
3	31.93932462451653	33.0	31.0	34.0	30.0	34.0
4	35.67489792786759	37.0	35.0	37.0	33.0	37.0
5	35.70608532039966	37.0	35.0	37.0	35.0	37.0
6	35.65773604225435	37.0	35.0	37.0	33.0	37.0
7	35.954048538579464	37.0	35.0	37.0	35.0	37.0
8	35.754446071352255	37.0	35.0	37.0	35.0	37.0
9	37.6077848733904	39.0	37.0	39.0	35.0	39.0
10	36.9623376418505	39.0	37.0	39.0	32.0	39.0
11	36.94140550493976	39.0	37.0	39.0	33.0	39.0
12	37.090564906690226	39.0	37.0	39.0	33.0	39.0
13	37.10038562973522	39.0	37.0	39.0	33.0	39.0
14	38.244453973248284	40.0	38.0	41.0	33.0	41.0
15	38.30102495192177	40.0	38.0	41.0	33.0	41.0
16	38.24853112214094	40.0	37.0	41.0	33.0	41.0
17	38.20260834273864	40.0	37.0	41.0	33.0	41.0
18	37.98149579593318	39.0	37.0	41.0	33.0	41.0
19	37.71298334578611	39.0	37.0	41.0	33.0	41.0
20	37.56409498910801	39.0	35.0	41.0	33.0	41.0
21	37.57819076697151	39.0	35.0	41.0	33.0	41.0
22	37.68660162540997	39.0	35.0	41.0	33.0	41.0
23	37.67060422973723	39.0	35.0	41.0	33.0	41.0
24	37.62699752330772	39.0	35.0	41.0	33.0	41.0
25	37.41091293429953	39.0	35.0	41.0	33.0	41.0
26	37.45697051918755	39.0	35.0	41.0	33.0	41.0
27	37.480452487519734	39.0	35.0	41.0	33.0	41.0
28	37.34954488807559	39.0	35.0	41.0	33.0	41.0
29	37.331300256273835	39.0	35.0	41.0	33.0	41.0
30	37.14366966345954	39.0	35.0	41.0	33.0	41.0
31	36.987285261314696	39.0	35.0	41.0	32.0	41.0
32	36.70813465060282	39.0	35.0	41.0	31.0	41.0
33	36.31624506490024	39.0	35.0	41.0	30.0	41.0
34	35.99372437440933	39.0	35.0	41.0	27.0	41.0
35	35.763004785594745	39.0	35.0	41.0	24.0	41.0
36	35.56019423462752	39.0	35.0	41.0	23.0	41.0
37	35.44447089563904	39.0	35.0	41.0	23.0	41.0
38	35.33506763936951	39.0	35.0	41.0	22.0	41.0
39	35.25371539693762	39.0	35.0	41.0	22.0	41.0
40	35.16867894926295	39.0	35.0	41.0	21.0	41.0
41	35.0286103131933	39.0	35.0	41.0	21.0	41.0
42	35.009892428090595	39.0	35.0	41.0	20.0	41.0
43	34.9730575461673	39.0	35.0	41.0	20.0	41.0
44	34.84994428517957	39.0	35.0	41.0	19.0	41.0
45	34.843023314178524	39.0	35.0	41.0	20.0	41.0
46	34.7476556035342	39.0	35.0	40.0	20.0	41.0
47	34.67731295380331	39.0	35.0	40.0	20.0	41.0
48	34.58847183640924	38.0	34.0	40.0	19.0	41.0
49	34.528956648563536	38.0	34.0	40.0	18.0	41.0
50	34.41802750892369	38.0	34.0	40.0	18.0	41.0
51	33.212701544956914	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	3.0
10	5.0
11	8.0
12	8.0
13	15.0
14	15.0
15	29.0
16	49.0
17	120.0
18	223.0
19	495.0
20	895.0
21	1452.0
22	2290.0
23	3450.0
24	5316.0
25	9497.0
26	14751.0
27	16603.0
28	15217.0
29	13410.0
30	13295.0
31	15058.0
32	18138.0
33	23373.0
34	35689.0
35	44460.0
36	51631.0
37	75101.0
38	140709.0
39	195929.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.53070911987793	19.90417337706385	23.062493815439815	20.502623687618403
2	38.15769660447927	24.86544548193678	22.715011164475598	14.261846749108347
3	26.225690196916396	25.07310329398639	35.24647175323139	13.454734755865832
4	23.207338007546237	22.4709271892626	38.922789441001804	15.398945362189357
5	24.29524695934754	26.38444516786868	33.06448721570742	16.255820657076356
6	22.260544585480304	32.32821980751498	32.171902808112996	13.239332798891725
7	70.63205129492141	4.586828356764152	20.63871986416196	4.142400484152468
8	71.99473398145135	6.285951117236316	17.5244263237827	4.194888577529646
9	66.40116678450197	6.5362017263706775	18.770946836445095	8.291684652682271
10	31.499739710684484	30.846936975567225	25.160583449615014	12.49273986413328
11	22.988350798292274	23.640866713227144	36.30784983816171	17.06293265031887
12	20.761478902224432	21.079849304676173	38.99736268842293	19.161309104676462
13	20.894563466852908	22.150549045534138	40.772779617410556	16.182107870202394
14	18.200748313855854	27.057468725844362	36.88564909558426	17.856133864715524
15	16.64288449321025	25.511938173041482	40.49269970930774	17.35247762444052
16	19.055042227101353	26.038109797634018	36.027483109876506	18.87936486538812
17	19.65306230738232	25.45127570446622	35.44695906072127	19.44870292743019
18	19.74828660793545	25.44711681182158	35.68258184055379	19.12201473968917
19	18.876783483746618	26.841493128505476	34.36277877129102	19.918944616456884
20	19.964118795183143	26.35203448725873	35.99220422744267	17.69164249011546
21	20.325368814902028	26.924670981398275	35.85438712980478	16.89557307389492
22	19.343439920493445	23.278899643052284	36.30784983816171	21.06981059829256
23	18.378003186572226	26.220527433633396	36.10836640131019	19.293102978484182
24	19.465768728282335	25.00082460802437	35.381850879318975	20.151555784374324
25	18.531882214423902	28.63440608861883	33.616042426441375	19.217669270515888
26	18.411561147911733	26.218232872174283	34.69362585167668	20.676580128237305
27	19.72419371261478	25.682165951289328	34.13188852446791	20.461751811627977
28	17.48484513861302	25.65118937159132	36.44882195780588	20.41514353198977
29	18.38804189295584	23.845943143635246	36.89611803224146	20.86989693116746
30	18.0359701190734	25.490139839179925	35.51264088248834	20.96124915925834
31	18.883093527759172	26.150256488948102	33.868444186943655	21.098205796349063
32	19.942177051230388	26.27129460591624	34.499305178108166	19.28722316474521
33	19.22096770261336	24.948336514647192	33.001817005855436	22.828878776884014
34	19.184541539449963	25.11827747271265	35.20660374787932	20.490577239958068
35	20.40381413478541	24.739818242050422	32.95291416475812	21.903453458406055
36	21.539622057045666	26.62207568897793	31.98403558864823	19.854266665328172
37	20.320062641527834	25.96138539884498	33.74668901951955	19.97186294010764
38	20.295109285659997	27.06722061204559	30.848514486570362	21.789155615724056
39	21.389758511747438	25.147963361589902	32.98733258664479	20.47494554001787
40	21.83820186691257	24.626237449824394	32.95119324366379	20.584367439599255
41	21.063930784553587	25.938870014527442	32.15125175498099	20.84594744593798
42	21.758035625934856	25.575612253531833	32.234429607873786	20.431922512659526
43	22.43120259400173	25.35160569108606	30.81395265459249	21.403239060319716
44	20.927834608010027	25.697941061320723	31.09188141132739	22.282342919341865
45	20.10236612309462	24.512226427324784	31.018885674909402	24.366521774671195
46	22.12889412176377	25.43420990361408	30.53516343731043	21.901732537311723
47	19.823290085630166	24.35232417564294	34.289352804599446	21.535032934127443
48	20.249935106933734	25.0520220105808	32.029783407739274	22.6682594747462
49	20.052602821450133	23.87075308941189	33.81108015046587	22.26556393867211
50	19.930847654026024	23.42618180670901	33.61819357780929	23.02477696145567
51	19.46691600901189	23.277035311866754	31.71456802729381	25.54148065182755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	477.0
2	651.0
3	10494.0
4	20337.0
5	13767.5
6	7198.0
7	7152.5
8	7107.0
9	7029.0
10	6951.0
11	6907.0
12	6863.0
13	6697.5
14	6532.0
15	6324.0
16	6116.0
17	5778.0
18	5440.0
19	5234.5
20	5029.0
21	4858.5
22	4688.0
23	4585.0
24	4482.0
25	4927.0
26	6172.5
27	6973.0
28	7697.5
29	8422.0
30	10175.0
31	11928.0
32	14045.0
33	16162.0
34	18126.5
35	20091.0
36	22037.0
37	23983.0
38	25932.0
39	27881.0
40	30497.5
41	33114.0
42	36336.0
43	39558.0
44	43726.5
45	47895.0
46	67802.0
47	87709.0
48	79239.5
49	70770.0
50	66378.0
51	61986.0
52	52123.0
53	42260.0
54	36833.0
55	31406.0
56	27560.0
57	23714.0
58	20989.5
59	18265.0
60	16343.0
61	14421.0
62	12112.5
63	9804.0
64	7991.0
65	6178.0
66	4826.0
67	3474.0
68	2626.0
69	1778.0
70	1429.0
71	1080.0
72	853.0
73	626.0
74	488.5
75	283.5
76	216.0
77	163.0
78	110.0
79	74.0
80	38.0
81	22.5
82	7.0
83	13.0
84	19.0
85	11.5
86	4.0
87	4.5
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	697301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.21371180447882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5330378347033	31.885298908709604
2	8.85704849378197	7.477777851096105
3	3.8144987277259776	4.830724499123265
4	2.252275410911452	3.8030762040212065
5	1.6449296277537602	3.471929262232293
6	1.279880593513993	3.2417106311247004
7	1.0330309623162786	3.052564992982596
8	0.8453510343252033	2.854832394929778
9	0.6925854415826505	2.6312942007852937
>10	3.9861057352079023	26.375216546927444
>50	0.03879555617497028	1.1074616724208886
>100	0.014973721335026556	1.1249878573347594
>500	0.0020418710911399096	0.6408595111186635
>1k	0.004083742182279819	3.0525944298947496
>5k	0.0010209355455699548	2.6138620947778706
>10k+	3.403118485233183E-4	1.8358089425208248
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12779	1.8326375553742213	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC	7518	1.078157065600078	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC	5481	0.7860307098369284	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG	5196	0.7451588338465025	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT	2994	0.4293698130362641	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT	2533	0.36325776099561025	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC	2489	0.3569477169830532	No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	2296	0.32926956938251917	No Hit
CGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTG	2076	0.2977193493197342	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCT	1709	0.24508784585136117	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT	1507	0.2161190074300768	No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	1248	0.17897579381070727	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT	1168	0.1675029865151491	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCT	1135	0.16277045350573138	No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT	1069	0.1533053874868959	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTC	1025	0.1469953434743389	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATCTGGT	939	0.1346620756316139	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCC	849	0.12175516742411097	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTA	764	0.10956530967258042	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTC	753	0.10798779866944117	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.86820182388954E-4	0.0	0.0	0.1639177342352872	0.0
2	2.86820182388954E-4	0.0	0.0	0.8141390877110459	0.0
3	4.3023027358343095E-4	0.0	0.0	1.3479114471368892	0.0
4	4.3023027358343095E-4	0.0	0.0	2.226441665794255	0.0
5	4.3023027358343095E-4	0.0	0.0	4.4791273782770995	0.0
6	5.73640364777908E-4	0.0	0.0	5.978479881715357	0.0
7	5.73640364777908E-4	0.0	0.0	7.095501082029138	0.0
8	5.73640364777908E-4	0.0	0.0	8.353350991895896	0.0
9	5.73640364777908E-4	0.0	0.0	8.984929033516373	0.0
10	5.73640364777908E-4	0.0	0.0	10.642749687724526	0.0
11	5.73640364777908E-4	0.0	0.0	11.648341247180198	0.0
12	5.73640364777908E-4	0.0	0.0	13.29038679135696	0.0
13	5.73640364777908E-4	0.0	0.0	13.79519031236152	0.0
14	5.73640364777908E-4	0.0	0.0	14.060929211344886	0.0
15	5.73640364777908E-4	0.0	0.0	14.427341994346774	0.0
16	5.73640364777908E-4	0.0	0.0	14.90575805857155	0.0
17	5.73640364777908E-4	0.0	0.0	15.506789750767602	0.0
18	7.17050455972385E-4	0.0	0.0	16.208782147164566	0.0
19	7.17050455972385E-4	0.0	0.0	16.779124079845	0.0
20	7.17050455972385E-4	0.0	0.0	17.24319913495033	0.0
21	8.604605471668619E-4	0.0	0.0	17.72018109826316	0.0
22	0.0010038706383613389	0.0	0.0	18.27876340346565	0.0
23	0.0010038706383613389	0.0	0.0	18.82142718854555	0.0
24	0.0010038706383613389	0.0	0.0	19.259401607053483	0.0
25	0.0010038706383613389	0.0	0.0	19.647469313825738	0.0
26	0.0010038706383613389	0.0	0.0	20.038548632513077	0.0
27	0.0010038706383613389	0.0	0.0	20.446550341961363	0.0
28	0.0010038706383613389	0.0	0.0	20.871617852261792	0.0
29	0.0010038706383613389	0.0	0.0	21.298979924021335	0.0
30	0.001147280729555816	0.0	0.0	21.853116516396792	0.0
31	0.001290690820750293	0.0	0.0	22.296540518370115	0.0
32	0.001290690820750293	0.0	0.0	22.72332894976488	0.0
33	0.001290690820750293	0.0	0.0	23.13979185459364	0.0
34	0.00143410091194477	0.0	0.0	23.565289595167652	0.0
35	0.00143410091194477	0.0	0.0	24.015023641153533	0.0
36	0.00143410091194477	0.0	0.0	24.45443216057341	0.0
37	0.00143410091194477	0.0	0.0	24.862290459930502	0.0
38	0.0015775110031392469	0.0	0.0	25.305140821539048	0.0
39	0.0017209210943337238	0.0	0.0	25.75659578861926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTA	20	7.0313766E-4	45.0	5
GTCGATG	20	7.0313766E-4	45.0	1
GTACGAG	20	7.0313766E-4	45.0	1
AGTTGCG	30	2.1642682E-6	44.999996	1
TTGCGCG	30	2.1642682E-6	44.999996	1
CGTTTTT	7895	0.0	44.145027	1
GCGTAAG	60	3.6379788E-12	41.249996	1
CGTAAGG	110	0.0	40.909092	2
CGAACGG	50	1.0804797E-9	40.499996	2
TAGTGCG	50	1.0804797E-9	40.499996	1
TATAGCG	45	1.9264917E-8	40.0	1
TCGACGG	40	3.455607E-7	39.375	2
GTTTTTT	8910	0.0	39.141415	2
CTAGCGG	75	0.0	39.000004	2
GCGATCG	35	6.2447543E-6	38.57143	9
TTACCGG	35	6.2447543E-6	38.57143	2
TGCGTAG	35	6.2447543E-6	38.57143	1
CGTTCTG	380	0.0	38.486843	1
CGTTTTC	195	0.0	38.076923	1
GGCGATC	125	0.0	37.8	8
>>END_MODULE