FastQCFastQC Report
Sat 14 Jan 2017
SRR2935376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603825
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116521.9296981741398582No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC59060.9780979588456921No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG29580.48987703390883125No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC27450.45460191280586265No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT17890.2962778950854966No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT14080.23318014325342606No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC13240.2192688278888751TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT12350.20452945803833894No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG12130.20088601830000413TruSeq Adapter, Index 15 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC9910.16412039912226223No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT8320.13778826646793357No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT7750.12834844532770256No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT7350.12172400943982113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG207.030623E-445.0000041
ATGCGAG207.030623E-445.0000041
ATAACGC207.030623E-445.00000411
CGAATAT351.2106466E-745.014
CGTTTTT68800.043.789971
CACGGGC451.925764E-840.0000044
CGGTCTA451.925764E-840.00000431
ACGGTCT451.925764E-840.00000430
GCTACGA403.4545883E-739.37500410
CTAAACG356.243363E-638.5714261
GTTTTTT80450.037.7563672
TCGACGG301.1392803E-437.4999962
CGTTTCT2450.036.7346951
CGGGCGA2050.036.2195136
CGGGTAC250.002106317836.0000046
ACGCCGA250.002106317836.00000412
GAGTACG250.002106317836.0000041
CCATACG250.002106317836.0000042
GTACTAG250.002106317836.0000041
CGTAAGG751.8189894E-1236.02
Produced by FastQC (version 0.11.5)