##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603825
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.925862625760775	33.0	31.0	34.0	30.0	34.0
2	31.990286920879395	33.0	31.0	34.0	30.0	34.0
3	32.041367946010844	33.0	31.0	34.0	30.0	34.0
4	35.751244151865194	37.0	35.0	37.0	35.0	37.0
5	35.76732165776508	37.0	35.0	37.0	35.0	37.0
6	35.70226472901917	37.0	35.0	37.0	33.0	37.0
7	35.970961785285475	37.0	35.0	37.0	35.0	37.0
8	35.79271974495922	37.0	35.0	37.0	35.0	37.0
9	37.62006044797748	39.0	37.0	39.0	35.0	39.0
10	37.088764128679664	39.0	37.0	39.0	33.0	39.0
11	37.03582826149961	39.0	37.0	39.0	33.0	39.0
12	37.08186312259347	39.0	37.0	39.0	33.0	39.0
13	37.046026580549	39.0	37.0	39.0	33.0	39.0
14	38.086828137291434	40.0	37.0	41.0	33.0	41.0
15	38.17798037510868	40.0	38.0	41.0	33.0	41.0
16	38.14502877489339	40.0	37.0	41.0	33.0	41.0
17	38.13323231068604	40.0	37.0	41.0	33.0	41.0
18	37.92110131246636	39.0	37.0	41.0	33.0	41.0
19	37.678415103713824	39.0	37.0	41.0	33.0	41.0
20	37.528234173808634	39.0	35.0	41.0	33.0	41.0
21	37.50025586883617	39.0	35.0	41.0	33.0	41.0
22	37.627254585351714	39.0	35.0	41.0	33.0	41.0
23	37.62491781559227	39.0	35.0	41.0	33.0	41.0
24	37.59712996315157	39.0	35.0	41.0	33.0	41.0
25	37.365566182254796	39.0	35.0	41.0	33.0	41.0
26	37.40873928704509	39.0	35.0	41.0	33.0	41.0
27	37.43391379952801	39.0	35.0	41.0	33.0	41.0
28	37.29147352295781	39.0	35.0	41.0	33.0	41.0
29	37.22301163416553	39.0	35.0	41.0	33.0	41.0
30	37.003524199892354	39.0	35.0	41.0	32.0	41.0
31	36.81466981327371	39.0	35.0	41.0	31.0	41.0
32	36.52470666169834	39.0	35.0	41.0	31.0	41.0
33	36.14409638554217	39.0	35.0	41.0	30.0	41.0
34	35.80619053533722	39.0	35.0	41.0	26.0	41.0
35	35.597853682772325	39.0	35.0	41.0	24.0	41.0
36	35.42188879228253	39.0	35.0	41.0	23.0	41.0
37	35.34438454850329	39.0	35.0	41.0	23.0	41.0
38	35.21506231110007	39.0	35.0	41.0	23.0	41.0
39	35.12688444499648	39.0	35.0	41.0	22.0	41.0
40	34.988327743965556	39.0	35.0	41.0	21.0	41.0
41	34.82958638678425	39.0	35.0	41.0	20.0	41.0
42	34.85642197656606	39.0	35.0	41.0	21.0	41.0
43	34.813391297147355	39.0	35.0	41.0	20.0	41.0
44	34.693368111621744	38.0	34.0	41.0	20.0	41.0
45	34.653921252018385	38.0	34.0	40.0	20.0	41.0
46	34.55851281414317	38.0	34.0	40.0	20.0	41.0
47	34.47270980830539	38.0	34.0	40.0	20.0	41.0
48	34.37465656440194	38.0	34.0	40.0	20.0	41.0
49	34.31846147476504	38.0	34.0	40.0	20.0	41.0
50	34.21396927917857	38.0	34.0	40.0	20.0	41.0
51	33.094898356311845	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	8.0
11	4.0
12	13.0
13	11.0
14	18.0
15	26.0
16	57.0
17	126.0
18	267.0
19	443.0
20	850.0
21	1298.0
22	2043.0
23	2852.0
24	4542.0
25	7764.0
26	12162.0
27	14278.0
28	13756.0
29	12629.0
30	12306.0
31	13822.0
32	16675.0
33	21805.0
34	34953.0
35	38933.0
36	45577.0
37	63916.0
38	115272.0
39	167352.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.032666749472114	19.02123959756552	21.62861756303565	20.317476089926718
2	38.85364136960212	24.150705916449304	22.16983397507556	14.825818738873018
3	27.792655156709312	24.173560220262495	33.35320664099698	14.680577982031217
4	24.64406077919927	22.45021322403014	36.06243530824329	16.843290688527304
5	24.418829959011305	25.706785906512646	32.586925019666296	17.287459114809756
6	23.1676396306877	31.6426116838488	31.763838860597026	13.425909824866475
7	71.46954829627789	4.989690721649485	19.377799859230738	4.162961122841883
8	72.87011965387322	5.938641162588498	17.245890779613298	3.945348403924978
9	66.59992547509627	6.79832732993831	18.856787976648864	7.7449592183165645
10	33.68856870782098	29.679625719372332	24.50958473067528	12.122220842131412
11	25.39494058709063	22.63751914875999	35.01378710719165	16.953753156957728
12	22.743675733863288	20.764459901461514	37.953049310644644	18.538815054030554
13	22.66931644102182	20.815633668695398	39.76731668943817	16.747733200844618
14	18.653417794890903	25.609903531652385	37.71225106612015	18.024427607336563
15	17.507721608081813	24.273920423963897	40.26067155218813	17.957686415766158
16	21.638719827764668	25.319090796174386	35.34103424005299	17.701155136007948
17	21.827019417877697	24.552643563946507	34.890903821471454	18.729433196704342
18	21.748354241709105	24.087773775514428	35.34269034902496	18.8211816337515
19	21.304351426323855	25.551111663147434	33.77352709808306	19.371009812445656
20	21.59152072206351	26.143170620626837	34.66915083012462	17.59615782718503
21	21.765246553223204	26.006872852233677	35.98476379745787	16.243116797085246
22	21.082929656771416	22.11451993541175	36.67917028940504	20.12338011841179
23	19.51774106736223	24.618059868339337	36.76677845402227	19.097420610276156
24	20.314660704674367	24.318304144412703	35.431292179025384	19.93574297188755
25	20.987537779985924	26.558357139899808	33.37192067238025	19.08218440773403
26	20.32724713286134	24.943154059537118	33.62298679253095	21.106612015070592
27	21.225355028360866	24.22224982403842	34.37353537862791	20.1788597689728
28	19.832898604728193	24.51223450503043	35.11828758332298	20.536579306918394
29	22.285264770421893	24.253881505403054	33.963814018962445	19.497039705212604
30	23.113319256407074	24.300086945721027	34.323686498571604	18.262907299300295
31	23.040450461640376	23.621082267213183	33.84258684221422	19.495880428932225
32	23.33805324390345	26.589657599470044	32.49997929863785	17.572309857988657
33	22.56630646296526	26.241543493561874	31.60120895954954	19.590941083923322
34	22.546101933507227	26.483004181675152	32.60828882540471	18.36260505941291
35	22.09431540595371	27.950647952635283	30.479526352834018	19.475510288576988
36	22.45700327081522	27.907092286672462	29.894919885728484	19.740984556783836
37	21.800852896120563	28.279882416262993	30.475386080404093	19.443878607212355
38	21.024303399163667	28.549000124208174	30.211236699374822	20.215459777253344
39	22.091168798906967	26.732248581956693	29.65743385914793	21.519148759988408
40	22.399039456796256	26.838239556162797	31.060158158406825	19.702562828634125
41	21.25350888088436	26.72164948453608	30.304475634496754	21.720366000082805
42	21.0614002401358	26.96857533225686	31.680702190204112	20.28932223740322
43	21.798037510868216	25.731461930195003	30.635034985302035	21.835465573634746
44	21.90717509212106	25.501925226679916	30.850825984349772	21.74007369684925
45	21.166231938061525	24.874922369891937	30.49111911563781	23.46772657640873
46	22.268538069804993	25.82387281083095	29.942118991429634	21.96547012793442
47	20.501469796712623	25.673663727073244	33.08872603817331	20.736140438040824
48	20.659793814432987	26.09431540595371	31.454146482838567	21.79174429677473
49	21.31329441477249	24.892311514097628	32.310851654038835	21.483542417091044
50	20.026497743551523	25.26079576036103	33.00972964021033	21.702976855877118
51	20.558936778039996	24.748726866227795	31.25856001324887	23.433776342483338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	500.0
2	694.0
3	10068.5
4	19443.0
5	12977.0
6	6511.0
7	6473.0
8	6435.0
9	6336.5
10	6238.0
11	6074.5
12	5911.0
13	5591.5
14	5272.0
15	5103.5
16	4935.0
17	4605.5
18	4276.0
19	4102.0
20	3928.0
21	3913.5
22	3899.0
23	4089.0
24	4279.0
25	4497.5
26	5518.0
27	6320.0
28	7191.5
29	8063.0
30	9476.5
31	10890.0
32	12322.5
33	13755.0
34	15361.5
35	16968.0
36	18355.0
37	19742.0
38	21368.5
39	22995.0
40	24644.5
41	26294.0
42	28536.5
43	30779.0
44	32912.0
45	35045.0
46	47291.5
47	59538.0
48	55174.5
49	50811.0
50	49365.0
51	47919.0
52	42415.5
53	36912.0
54	33844.5
55	30777.0
56	28482.0
57	26187.0
58	24065.0
59	21943.0
60	20227.5
61	18512.0
62	16339.5
63	14167.0
64	12078.5
65	9990.0
66	8492.5
67	6995.0
68	5878.0
69	4761.0
70	4024.5
71	3288.0
72	2667.5
73	2047.0
74	1560.0
75	843.5
76	614.0
77	479.0
78	344.0
79	234.0
80	124.0
81	101.0
82	78.0
83	56.5
84	35.0
85	20.0
86	5.0
87	4.5
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.510565459630506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.2994383423422	28.565916913196315
2	8.61768633222486	6.809793198798671
3	4.146735313330225	4.915195711232859
4	2.8003476059789176	4.425732695829984
5	2.077338332467832	4.103840608338498
6	1.6679081855599969	3.953999732773308
7	1.3550546573629818	3.7477283018782095
8	1.151433089591591	3.6395017966954524
9	0.9662949079034993	3.4360972390825952
>10	4.864817357319177	28.490247865879187
>50	0.031515402359796364	0.8628346047774228
>100	0.014707187736495929	1.2003644761145595
>500	0.002941437547299186	0.8297955258119735
>1k	0.002941437547299186	2.1038753307501654
>5k	4.2020536389988363E-4	0.9805466937666099
>10k+	4.2020536389988363E-4	1.9345293050742527
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11652	1.9296981741398582	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	5906	0.9780979588456921	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	2958	0.48987703390883125	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	2745	0.45460191280586265	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	1789	0.2962778950854966	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	1408	0.23318014325342606	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1324	0.2192688278888751	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	1235	0.20452945803833894	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	1213	0.20088601830000413	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	991	0.16412039912226223	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	832	0.13778826646793357	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	775	0.12834844532770256	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	735	0.12172400943982113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.280544859851778E-4	0.0	0.0	0.08976110628079327	0.0
2	8.280544859851778E-4	0.0	0.0	0.46586345381526106	0.0
3	8.280544859851778E-4	0.0	0.0	0.8321947584151037	0.0
4	8.280544859851778E-4	0.0	0.0	1.462675444044218	0.0
5	8.280544859851778E-4	0.0	0.0	3.1972839812859686	0.0
6	8.280544859851778E-4	0.0	0.0	4.433734939759036	0.0
7	8.280544859851778E-4	0.0	0.0	5.314122469258478	0.0
8	8.280544859851778E-4	0.0	0.0	6.322527222291226	0.0
9	8.280544859851778E-4	0.0	0.0	6.9124332381070674	0.0
10	8.280544859851778E-4	0.0	0.0	8.110462468430423	0.0
11	8.280544859851778E-4	0.0	0.0	8.789798368732663	0.0
12	8.280544859851778E-4	0.0	0.0	9.812942491615948	0.0
13	8.280544859851778E-4	0.0	0.0	10.175299134683062	0.0
14	8.280544859851778E-4	0.0	0.0	10.429180640086118	0.0
15	8.280544859851778E-4	0.0	0.0	10.67759698588167	0.0
16	8.280544859851778E-4	0.0	0.0	10.958638678425041	0.0
17	8.280544859851778E-4	0.0	0.0	11.2681654452863	0.0
18	8.280544859851778E-4	0.0	0.0	11.630687699250611	0.0
19	9.936653831822133E-4	0.0	0.0	11.936571026373535	0.0
20	9.936653831822133E-4	0.0	0.0	12.192108640748561	0.0
21	9.936653831822133E-4	0.0	0.0	12.458410963441395	0.0
22	9.936653831822133E-4	0.0	0.0	12.778868049517659	0.0
23	9.936653831822133E-4	0.0	0.0	13.083426489463006	0.0
24	9.936653831822133E-4	0.0	0.0	13.337307994866062	0.0
25	9.936653831822133E-4	0.0	0.0	13.574959632343809	0.0
26	0.001159276280379249	0.0	0.0	13.821057425578603	0.0
27	0.001159276280379249	0.0	0.0	14.112035771953794	0.0
28	0.001159276280379249	0.0	0.0	14.369560717095185	0.0
29	0.001159276280379249	0.0	0.0	14.656564401937647	0.0
30	0.001159276280379249	0.0	0.0	15.04690928663106	0.0
31	0.001159276280379249	0.0	0.0	15.344015236202543	0.0
32	0.001159276280379249	0.0	0.0	15.650395396017059	0.0
33	0.001159276280379249	0.0	0.0	15.94187057508384	0.0
34	0.001159276280379249	0.0	0.0	16.25090050925351	0.0
35	0.001159276280379249	0.0	0.0	16.554962116507266	0.0
36	0.0014904980747733202	0.0	0.0	16.832691591106695	0.0
37	0.0014904980747733202	0.0	0.0	17.12963192978098	0.0
38	0.0014904980747733202	0.0	0.0	17.483873638885438	0.0
39	0.0016561089719703556	0.0	0.0	18.0166438951683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGCG	20	7.030623E-4	45.000004	1
ATGCGAG	20	7.030623E-4	45.000004	1
ATAACGC	20	7.030623E-4	45.000004	11
CGAATAT	35	1.2106466E-7	45.0	14
CGTTTTT	6880	0.0	43.78997	1
CACGGGC	45	1.925764E-8	40.000004	4
CGGTCTA	45	1.925764E-8	40.000004	31
ACGGTCT	45	1.925764E-8	40.000004	30
GCTACGA	40	3.4545883E-7	39.375004	10
CTAAACG	35	6.243363E-6	38.571426	1
GTTTTTT	8045	0.0	37.756367	2
TCGACGG	30	1.1392803E-4	37.499996	2
CGTTTCT	245	0.0	36.734695	1
CGGGCGA	205	0.0	36.219513	6
CGGGTAC	25	0.0021063178	36.000004	6
ACGCCGA	25	0.0021063178	36.000004	12
GAGTACG	25	0.0021063178	36.000004	1
CCATACG	25	0.0021063178	36.000004	2
GTACTAG	25	0.0021063178	36.000004	1
CGTAAGG	75	1.8189894E-12	36.0	2
>>END_MODULE