##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935375.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626582
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75864611495383	31.0	31.0	34.0	30.0	34.0
2	31.816494888139143	33.0	31.0	34.0	30.0	34.0
3	31.898445853854724	33.0	31.0	34.0	30.0	34.0
4	35.64256234618932	37.0	35.0	37.0	33.0	37.0
5	35.652257805043874	37.0	35.0	37.0	33.0	37.0
6	35.585872240185644	37.0	35.0	37.0	33.0	37.0
7	35.87914430992272	37.0	35.0	37.0	35.0	37.0
8	35.6566530797246	37.0	35.0	37.0	33.0	37.0
9	37.502593435496074	39.0	37.0	39.0	35.0	39.0
10	36.7259943630682	39.0	35.0	39.0	32.0	39.0
11	36.829051903821046	39.0	37.0	39.0	32.0	39.0
12	36.922903945533065	39.0	37.0	39.0	33.0	39.0
13	36.85600448145654	39.0	37.0	39.0	33.0	39.0
14	37.88958189031922	40.0	37.0	41.0	33.0	41.0
15	37.95002729092122	40.0	37.0	41.0	33.0	41.0
16	37.97331075581488	40.0	37.0	41.0	33.0	41.0
17	37.92822008930994	40.0	37.0	41.0	33.0	41.0
18	37.77864668949954	39.0	37.0	41.0	33.0	41.0
19	37.56027942073025	39.0	37.0	41.0	33.0	41.0
20	37.40881799987871	39.0	35.0	41.0	32.0	41.0
21	37.47531049407739	39.0	35.0	41.0	33.0	41.0
22	37.60938233144265	39.0	35.0	41.0	33.0	41.0
23	37.6001401253148	39.0	35.0	41.0	33.0	41.0
24	37.56107740088289	39.0	35.0	41.0	33.0	41.0
25	37.358904341331225	39.0	35.0	41.0	33.0	41.0
26	37.410066040837435	39.0	35.0	41.0	33.0	41.0
27	37.408669575570315	39.0	35.0	41.0	33.0	41.0
28	37.27863232585679	39.0	35.0	41.0	33.0	41.0
29	37.22449894826216	39.0	35.0	41.0	33.0	41.0
30	37.050791436715386	39.0	35.0	41.0	32.0	41.0
31	36.97849922276733	39.0	35.0	41.0	32.0	41.0
32	36.74067081403551	39.0	35.0	41.0	31.0	41.0
33	36.33342004717659	39.0	35.0	41.0	30.0	41.0
34	36.12617662173506	39.0	35.0	41.0	29.0	41.0
35	35.887345949931536	39.0	35.0	41.0	27.0	41.0
36	35.7845341870657	39.0	35.0	41.0	26.0	41.0
37	35.710221487371165	39.0	35.0	41.0	25.0	41.0
38	35.63006597699902	39.0	35.0	41.0	25.0	41.0
39	35.51455834990472	39.0	35.0	41.0	24.0	41.0
40	35.35542195594511	39.0	35.0	41.0	23.0	41.0
41	35.23449125573349	39.0	35.0	41.0	23.0	41.0
42	35.32464066953727	39.0	35.0	41.0	23.0	41.0
43	35.294751524940075	39.0	35.0	41.0	23.0	41.0
44	35.202548110223404	39.0	35.0	41.0	23.0	41.0
45	35.184579193146305	39.0	35.0	40.0	23.0	41.0
46	34.99612979625971	39.0	35.0	40.0	23.0	41.0
47	34.857239754732824	38.0	34.0	40.0	23.0	41.0
48	34.77766996179271	38.0	34.0	40.0	23.0	41.0
49	34.697678835332006	38.0	34.0	40.0	23.0	41.0
50	34.51602663338557	38.0	34.0	40.0	22.0	41.0
51	33.345914820406584	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	5.0
11	7.0
12	10.0
13	7.0
14	20.0
15	22.0
16	45.0
17	92.0
18	211.0
19	466.0
20	985.0
21	1633.0
22	2246.0
23	3175.0
24	4712.0
25	7653.0
26	11476.0
27	12491.0
28	12092.0
29	10999.0
30	11889.0
31	13982.0
32	17889.0
33	23813.0
34	37126.0
35	42721.0
36	47431.0
37	69922.0
38	128234.0
39	165173.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11021063484109	18.731147718893936	23.021886999626545	16.13675464663843
2	34.401722360361454	28.73446731632891	22.408399858278724	14.455410465030912
3	26.751965425115948	28.020275079718214	31.24188055194691	13.985878943218925
4	23.743739845702557	22.331314975533928	37.76281476327121	16.162130415492307
5	26.402609714291188	26.36765818360566	30.400649875036308	16.82908222706685
6	21.891468315400058	34.3480980941042	30.633819675637024	13.126613914858709
7	71.51673683572142	4.82155567826717	19.597754164658415	4.063953321352991
8	73.04231529153407	7.686623618297365	14.87147731661618	4.399583773552384
9	68.33423239097198	5.426424633966504	15.833202996575068	10.406139978486454
10	35.252688395134236	23.45997810342461	25.20372433296839	16.083609168472762
11	27.55265871027256	24.94278482305588	29.83169002620567	17.672866440465892
12	23.23590527656396	21.07960330810652	34.41895873165843	21.265532683671093
13	22.510700913846872	22.480377668046643	37.48958635900808	17.51933505909841
14	19.413739941460175	27.78040224583534	33.79414027214315	19.01171754056133
15	18.028446396481225	25.060087905493617	38.900255672840906	18.011210025184255
16	20.7061485966721	25.08227175373694	32.88236814973938	21.329211499851574
17	21.445397410075618	25.64803968195703	33.086012684692506	19.820550223274847
18	21.36113070595708	25.26149809601936	33.00461870912347	20.372752488900094
19	20.666249589040223	27.154785806167432	31.118991608440716	21.059972996351632
20	21.329051903821046	26.989923106632492	33.97560095885295	17.70542403069351
21	20.474574756376658	28.477198515118534	33.61443514176916	17.433791586735655
22	19.38628942420944	23.102642591073476	34.96302159972677	22.54804638499031
23	19.620576397023854	26.71158762939248	34.57520324554488	19.09263272803879
24	20.539530340801363	25.376886026090762	33.04387933263324	21.03970430047464
25	19.602063257482662	30.086724482988657	30.72143789639664	19.58977436313204
26	19.35006112527969	25.52993861936666	32.42257198578957	22.697428269564078
27	21.324742810996806	26.51257137932465	31.261351267671273	20.901334542007273
28	18.426319300586353	25.973136796141606	33.63566141382932	21.964882489442722
29	22.589700948957997	24.752546354667064	31.33891493850765	21.318837757867286
30	20.62363744888937	28.54917632488645	31.532345327507016	19.294840898717165
31	21.792518776472992	25.548611354938377	29.67720106865502	22.98166879993361
32	24.546827071317082	27.503183940809024	28.57870159053404	19.371287397339852
33	20.729928405220704	27.403276825698793	29.04823311234603	22.818561656734474
34	21.79922180975515	25.8357246138574	31.63017769422039	20.73487588216706
35	20.572247527059506	29.269433210657187	28.623388479081747	21.53493078320156
36	21.400231733436325	28.25408326444105	27.504460709053248	22.84122429306938
37	20.7768496381958	28.260467105662144	30.34686601274853	20.615817243393522
38	19.81464517014533	28.64844505587457	27.26889696799461	24.26801280598549
39	22.53272516605967	25.606544714019876	29.762584944987246	22.098145174933208
40	21.180627595430444	27.958671011934594	30.720001532121895	20.14069986051307
41	19.27329543459595	27.094139314566966	29.782853640864243	23.849711609972836
42	20.339396918519842	27.26985454417778	30.709627790137606	21.681120747164776
43	21.633401534037045	25.69591849111529	28.49746721099553	24.173212763852135
44	21.856676380745057	25.198457663960983	29.233683699819018	23.71118225547494
45	20.814993089491878	24.376059318652626	28.87570980334577	25.93323778850972
46	23.857691411499214	26.303181387272534	28.085390260173448	21.753736941054804
47	19.443424803138296	25.4142314972342	33.43185728284566	21.71048641678184
48	20.304285791803785	26.235512670328866	29.624534378580936	23.835667159286412
49	20.276196890430942	24.136505676830804	32.791398412338694	22.795899020399567
50	20.456380808896522	23.905410624626946	31.62459183315193	24.013616733324607
51	19.97073008800125	23.98121873912752	29.499570686677878	26.54848048619335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	380.5
2	531.0
3	6852.0
4	13173.0
5	8972.5
6	4772.0
7	4755.0
8	4738.0
9	4699.5
10	4661.0
11	4577.5
12	4494.0
13	4344.0
14	4194.0
15	4028.0
16	3862.0
17	3626.5
18	3391.0
19	3253.0
20	3115.0
21	3052.0
22	2989.0
23	3096.0
24	3203.0
25	3449.5
26	4188.5
27	4681.0
28	5488.5
29	6296.0
30	7635.5
31	8975.0
32	10547.5
33	12120.0
34	14157.5
35	16195.0
36	18005.5
37	19816.0
38	21616.5
39	23417.0
40	26267.5
41	29118.0
42	31185.0
43	33252.0
44	37124.0
45	40996.0
46	63862.5
47	86729.0
48	75294.0
49	63859.0
50	60875.5
51	57892.0
52	50532.5
53	43173.0
54	38363.0
55	33553.0
56	30076.0
57	26599.0
58	23592.5
59	20586.0
60	18176.0
61	15766.0
62	13229.5
63	10693.0
64	8775.5
65	6858.0
66	5408.0
67	3958.0
68	2912.0
69	1866.0
70	1667.5
71	1469.0
72	1076.0
73	683.0
74	579.0
75	385.0
76	295.0
77	201.5
78	108.0
79	80.5
80	53.0
81	39.0
82	25.0
83	21.0
84	17.0
85	10.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.307033941525624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52584403818821	29.686969150714347
2	8.716541404293283	6.852427776625392
3	3.6345808776314525	4.285937817608385
4	2.220273232754769	3.490894212774102
5	1.5344163487423939	3.0156677750224548
6	1.192668792010991	2.812816361314464
7	0.9680888369340767	2.663689054024587
8	0.7977832500369453	2.508679462974627
9	0.6693157214166158	2.3677934201387663
>10	4.667291682678769	31.37363397653874
>50	0.047170636555563625	1.2899044947871652
>100	0.018705597231520675	1.3044498849992492
>500	0.0024398605084592186	0.6737233507256262
>1k	0.0032531473446122915	2.2906593924671292
>5k	0.0012199302542296093	3.6566474720166426
>10k+	4.0664341807653643E-4	1.7261063972683361
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	10799	1.7234775336667827	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7967	1.2715015752128214	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	7607	1.214047004222911	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	7303	1.1655298109425423	No Hit
GAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	3305	0.5274648808934824	No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	2745	0.4380911037980663	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	1907	0.30434963021599726	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	1620	0.25854556945459656	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC	1387	0.22135969434168234	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACT	1228	0.19598392548780527	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACGATACTCGTA	1119	0.17858795816030462	No Hit
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1020	0.1627879511380793	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	896	0.14299804335266572	No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	705	0.11251520152190775	TruSeq Adapter, Index 23 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	693	0.11060004915557739	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	682	0.1088444928197746	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	670	0.10692934045344424	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1919206105505743E-4	0.0	0.0	0.122250559384087	0.0
2	3.1919206105505743E-4	0.0	0.0	0.7885639868365194	0.0
3	3.1919206105505743E-4	0.0	0.0	1.1559540491108906	0.0
4	3.1919206105505743E-4	0.0	0.0	2.091825172124319	0.0
5	3.1919206105505743E-4	0.0	0.0	4.721967755217992	0.0
6	3.1919206105505743E-4	0.0	0.0	5.900584440663792	0.0
7	3.1919206105505743E-4	0.0	0.0	6.889601041842887	0.0
8	3.1919206105505743E-4	0.0	0.0	8.077793489120339	0.0
9	3.1919206105505743E-4	0.0	0.0	8.54014318955859	0.0
10	3.1919206105505743E-4	0.0	0.0	10.550893578174923	0.0
11	3.1919206105505743E-4	0.0	0.0	11.512459662103284	0.0
12	3.1919206105505743E-4	0.0	0.0	13.526721163391224	0.0
13	3.1919206105505743E-4	0.0	0.0	13.967684995738786	0.0
14	3.1919206105505743E-4	0.0	0.0	14.224315412827051	0.0
15	3.1919206105505743E-4	0.0	0.0	14.667992377693581	0.0
16	3.1919206105505743E-4	0.0	0.0	15.188754225304908	0.0
17	3.1919206105505743E-4	0.0	0.0	15.75436255749447	0.0
18	3.1919206105505743E-4	0.0	0.0	16.389714355024562	0.0
19	4.7878809158258614E-4	0.0	0.0	17.00336109240291	0.0
20	4.7878809158258614E-4	0.0	0.0	17.445282500933637	0.0
21	4.7878809158258614E-4	0.0	0.0	17.935082718622624	0.0
22	4.7878809158258614E-4	0.0	0.0	18.521438534780764	0.0
23	4.7878809158258614E-4	0.0	0.0	19.06087311796381	0.0
24	4.7878809158258614E-4	0.0	0.0	19.51348746053988	0.0
25	4.7878809158258614E-4	0.0	0.0	19.92428764311774	0.0
26	4.7878809158258614E-4	0.0	0.0	20.327746408291333	0.0
27	4.7878809158258614E-4	0.0	0.0	20.77174256521892	0.0
28	4.7878809158258614E-4	0.0	0.0	21.185734668407328	0.0
29	4.7878809158258614E-4	0.0	0.0	21.61297324212952	0.0
30	4.7878809158258614E-4	0.0	0.0	22.128628016763965	0.0
31	6.383841221101149E-4	0.0	0.0	22.567995888806255	0.0
32	6.383841221101149E-4	0.0	0.0	23.0094385092454	0.0
33	6.383841221101149E-4	0.0	0.0	23.456945778844588	0.0
34	6.383841221101149E-4	0.0	0.0	23.90030355165006	0.0
35	6.383841221101149E-4	0.0	0.0	24.33568152292916	0.0
36	6.383841221101149E-4	0.0	0.0	24.749514030087045	0.0
37	6.383841221101149E-4	0.0	0.0	25.178348564114515	0.0
38	6.383841221101149E-4	0.0	0.0	25.670383126230885	0.0
39	7.979801526376436E-4	0.0	0.0	26.285625823914508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	30	2.1639153E-6	45.000004	1
TCTAGCG	40	6.8066583E-9	45.0	1
AGTGCGC	20	7.0308277E-4	45.0	13
TGTAACG	20	7.0308277E-4	45.0	1
AATCGCG	20	7.0308277E-4	45.0	1
GCGTAGT	25	3.8887683E-5	44.999996	18
CGTTTTT	4945	0.0	44.180992	1
CGATGAA	535	0.0	42.056072	19
CTAGCGG	65	0.0	41.53846	2
ACGACAC	55	6.002665E-11	40.909092	37
CGACACT	50	1.0804797E-9	40.499996	38
TACGAAT	50	1.0804797E-9	40.499996	12
CCGATGA	540	0.0	40.416664	18
CGCGGGT	45	1.925946E-8	40.0	4
CAACGAC	45	1.925946E-8	40.0	12
GCGCAAT	45	1.925946E-8	40.0	19
TACGGCT	950	0.0	39.55263	7
GGCTACG	40	3.4548611E-7	39.375	1
ACGGCTG	955	0.0	39.34555	8
TTTAGCG	35	6.243741E-6	38.571426	1
>>END_MODULE