##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384625
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.834059148521288	33.0	31.0	34.0	30.0	34.0
2	31.87196360090998	33.0	31.0	34.0	30.0	34.0
3	31.944857978550537	33.0	31.0	34.0	30.0	34.0
4	35.691673708157296	37.0	35.0	37.0	33.0	37.0
5	35.703597010074745	37.0	35.0	37.0	35.0	37.0
6	35.65687097822554	37.0	35.0	37.0	33.0	37.0
7	35.922397140071496	37.0	35.0	37.0	35.0	37.0
8	35.720293792655184	37.0	35.0	37.0	35.0	37.0
9	37.534533636659084	39.0	37.0	39.0	35.0	39.0
10	36.92688722781931	39.0	37.0	39.0	32.0	39.0
11	36.90638154046149	39.0	37.0	39.0	33.0	39.0
12	36.929164770880725	39.0	37.0	39.0	33.0	39.0
13	36.84701462463438	39.0	37.0	39.0	33.0	39.0
14	37.914155346116345	40.0	37.0	41.0	33.0	41.0
15	38.026755931101725	40.0	37.0	41.0	33.0	41.0
16	38.01332466688333	40.0	37.0	41.0	33.0	41.0
17	37.96779460513487	40.0	37.0	41.0	33.0	41.0
18	37.90903867403315	39.0	37.0	41.0	33.0	41.0
19	37.79195320116997	39.0	37.0	41.0	33.0	41.0
20	37.78085147871303	39.0	36.0	41.0	33.0	41.0
21	37.77663178420539	39.0	36.0	41.0	33.0	41.0
22	37.85566460838479	40.0	36.0	41.0	33.0	41.0
23	37.85390705232369	40.0	36.0	41.0	33.0	41.0
24	37.85964770880728	40.0	36.0	41.0	33.0	41.0
25	37.618859928501784	39.0	36.0	41.0	33.0	41.0
26	37.67639649008775	39.0	36.0	41.0	33.0	41.0
27	37.67352876178096	40.0	36.0	41.0	33.0	41.0
28	37.561315567110825	40.0	36.0	41.0	33.0	41.0
29	37.524929476763084	40.0	36.0	41.0	33.0	41.0
30	37.4163223919402	39.0	36.0	41.0	33.0	41.0
31	37.4254351641209	40.0	36.0	41.0	33.0	41.0
32	37.34636854078648	40.0	36.0	41.0	33.0	41.0
33	37.20104257393565	40.0	35.0	41.0	32.0	41.0
34	37.061241468963274	40.0	35.0	41.0	32.0	41.0
35	36.985619759506015	40.0	35.0	41.0	32.0	41.0
36	36.909868053298666	40.0	35.0	41.0	31.0	41.0
37	36.816023399415016	39.0	35.0	41.0	31.0	41.0
38	36.79601949951251	39.0	35.0	41.0	31.0	41.0
39	36.686835229119275	39.0	35.0	41.0	31.0	41.0
40	36.59442833929152	39.0	35.0	41.0	31.0	41.0
41	36.43272278193045	39.0	35.0	41.0	30.0	41.0
42	36.4859278518037	39.0	35.0	41.0	30.0	41.0
43	36.45395385115372	39.0	35.0	41.0	30.0	41.0
44	36.35417094572636	39.0	35.0	41.0	30.0	41.0
45	36.35902502437439	39.0	35.0	41.0	30.0	41.0
46	36.24080857978551	39.0	35.0	41.0	30.0	41.0
47	36.175082222944425	39.0	35.0	40.0	30.0	41.0
48	36.09313747156321	39.0	35.0	40.0	30.0	41.0
49	36.017786155346116	39.0	35.0	40.0	29.0	41.0
50	35.85590640233994	38.0	35.0	40.0	29.0	41.0
51	34.603850503737405	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	1.0
11	4.0
12	6.0
13	8.0
14	8.0
15	20.0
16	23.0
17	36.0
18	131.0
19	233.0
20	442.0
21	719.0
22	1062.0
23	1449.0
24	1866.0
25	2612.0
26	3574.0
27	4118.0
28	4523.0
29	4912.0
30	5886.0
31	7595.0
32	9896.0
33	13795.0
34	24441.0
35	29552.0
36	30167.0
37	43975.0
38	80860.0
39	112653.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67533311667208	20.353851153721155	24.408969775755608	12.561845953851153
2	30.821189470263242	28.73864153396165	24.486967825804353	15.953201169970752
3	29.339746506337345	28.283652908677283	27.147221319467015	15.22937926551836
4	27.243418914527133	24.69366265843354	31.18232044198895	16.880597985050375
5	27.55619109522262	28.477868053298668	25.693077673058173	18.27286317842054
6	24.883977900552487	35.88664283392915	25.003054923626912	14.226324341891452
7	76.69652258693532	5.9028924276893076	12.89281767955801	4.507767305817355
8	77.55320116997075	8.31771205719857	9.5480012999675	4.581085472863179
9	71.53643158921027	8.114397140071498	11.147481312967175	9.201689957751057
10	38.02378940526487	30.118947026324346	18.20942476438089	13.647838804029899
11	29.665258368540787	25.10913227169321	27.19974000649984	18.02586935326617
12	27.469093272668182	21.123431914202147	30.975885602859925	20.431589210269742
13	24.939876503087422	23.836724081897952	31.92382190445239	19.299577510562234
14	19.149821254468637	30.079688007799803	29.19831004224894	21.57218069548261
15	18.02430939226519	26.518297042573934	35.422294442638936	20.035099122521938
16	20.505687357816054	25.80409489762756	31.037764055898602	22.652453688657783
17	19.67994800129997	26.455378615534613	30.030029249268768	23.834644133896653
18	21.125511862203446	26.4811179720507	30.047968800779977	22.345401364965873
19	22.151186220344492	28.232434189145273	27.68436789080273	21.932011699707505
20	23.92174195645109	26.376600584985376	29.908872278193044	19.79278518037049
21	23.253558661033473	28.500747481312967	29.241208969775755	19.004484887877805
22	20.57822554436139	25.46142346441339	29.448163795905103	24.512187195320116
23	20.645823854403638	28.026519337016577	29.085212869678255	22.242443938901527
24	21.708417289567763	25.788495287617806	29.505622359441013	22.997465063373415
25	20.508807279818004	29.88651283717907	27.118882027949304	22.485797855053626
26	19.417874553136173	27.979720506987327	28.77400064998375	23.828404289892752
27	21.00019499512512	28.509847253818656	28.07747806304842	22.4124796880078
28	18.64595385115372	27.65940851478713	31.11758206044849	22.57705557361066
29	20.753461163470913	25.12421189470263	31.067143321416964	23.05518362040949
30	20.49580760480988	28.450568735781605	29.956191095222618	21.097432564185894
31	22.3001624959376	27.342476438089047	26.952486187845302	23.404874878128044
32	24.76698082547936	28.611764705882354	26.025609359766005	20.59564510887228
33	23.683587910302244	26.707572310692235	26.64517387065323	22.96366590835229
34	21.76327591810205	25.96685082872928	29.897172570685733	22.372700682482936
35	21.416444588885277	27.391355216119596	28.221514462138447	22.97068573285668
36	22.955866103347418	27.818784530386743	27.718167045823854	21.507182320441988
37	21.7268768280793	28.85901852453689	28.968995775105622	20.44510887227819
38	22.17900552486188	27.798245043873905	26.254663633409166	23.768085797855054
39	24.252713682157946	25.381345466363342	28.6377640558986	21.728176795580108
40	23.30945726356841	26.128566785830355	30.092687682807927	20.469288267793306
41	20.54780630484238	26.81234969125772	29.406824829379264	23.233019174520635
42	20.820279493012674	27.672408189795256	29.356646083847902	22.150666233344165
43	23.421254468638285	26.118687032824177	27.183880402989924	23.27617809554761
44	21.80305492362691	26.311602209944752	27.97322066948326	23.912122196945077
45	21.550341241468963	24.999155021124473	27.558531036724084	25.89197270068248
46	23.40721481962951	27.81254468638284	27.111862203444915	21.668378290542737
47	20.815339616509586	25.27422814429639	31.527331816704585	22.383100422489438
48	21.16659083522912	25.482742931426717	29.178550536236592	24.17211569710757
49	20.905557361065974	24.125836854078646	31.613129671758205	23.355476113097172
50	20.674683132921675	23.979460513487165	31.021384465388362	24.324471888202794
51	20.857718557036073	23.84998375040624	28.586805329866756	26.70549236269093
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	215.0
1	406.0
2	597.0
3	2011.5
4	3426.0
5	2326.0
6	1226.0
7	1198.5
8	1171.0
9	1176.5
10	1182.0
11	1190.5
12	1199.0
13	1189.5
14	1180.0
15	1128.0
16	1076.0
17	1098.5
18	1121.0
19	1167.5
20	1214.0
21	1285.5
22	1357.0
23	1484.5
24	1612.0
25	2190.0
26	3155.0
27	3542.0
28	4493.5
29	5445.0
30	6474.5
31	7504.0
32	7962.5
33	8421.0
34	10495.5
35	12570.0
36	13287.0
37	14004.0
38	14382.5
39	14761.0
40	16736.0
41	18711.0
42	20449.0
43	22187.0
44	24193.5
45	26200.0
46	35661.5
47	45123.0
48	40908.0
49	36693.0
50	35091.5
51	33490.0
52	28673.0
53	23856.0
54	21375.0
55	18894.0
56	17327.0
57	15760.0
58	15399.5
59	15039.0
60	14250.5
61	13462.0
62	11893.5
63	10325.0
64	8751.0
65	7177.0
66	5635.0
67	4093.0
68	3527.0
69	2961.0
70	2446.5
71	1932.0
72	1680.5
73	1429.0
74	1102.5
75	643.5
76	511.0
77	357.0
78	203.0
79	156.5
80	110.0
81	91.5
82	73.0
83	42.0
84	11.0
85	10.5
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	384625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.60172809476788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23907476796565	27.14242810085792
2	8.428635927652657	6.001480090121631
3	2.9979840829099382	3.20200242456605
4	1.6577078514850674	2.360690567565329
5	1.1761295263366534	2.093612180043283
6	0.9258528567970726	1.9777176998072064
7	0.7456607145133043	1.8582767006337098
8	0.6356856823947018	1.8105207054682517
9	0.5844140601298408	1.8725535417151729
>10	6.500608815250025	43.17307831134119
>50	0.06363092680518039	1.489937080673059
>100	0.03510671823734089	2.042739671073077
>500	0.005851119706223483	1.3672945841688628
>1k	0.003656949816389677	3.607668341965262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	3559	0.9253168670783231	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	3535	0.9190770230744232	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	3282	0.8532986675333117	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2047	0.5322066948326292	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1432	0.3723106922326942	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	795	0.20669483262918428	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTT	783	0.20357491062723432	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	664	0.1726356841078973	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	658	0.17107572310692232	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	644	0.1674358141046474	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	595	0.15469613259668508	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	579	0.15053623659408516	No Hit
CGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTG	533	0.13857653558661032	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	498	0.12947676308092299	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	488	0.126876828079298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.199870003249918E-4	0.0	0.0	0.16249593760155995	0.0
2	5.199870003249918E-4	0.0	0.0	0.8756581085472863	0.0
3	5.199870003249918E-4	0.0	0.0	1.2373090672733182	0.0
4	5.199870003249918E-4	0.0	0.0	1.8209944751381215	0.0
5	5.199870003249918E-4	0.0	0.0	3.6438089047773805	0.0
6	5.199870003249918E-4	0.0	0.0	4.581085472863179	0.0
7	5.199870003249918E-4	0.0	0.0	5.394865128371791	0.0
8	5.199870003249918E-4	0.0	0.0	6.469418264543386	0.0
9	5.199870003249918E-4	0.0	0.0	6.847708807279818	0.0
10	5.199870003249918E-4	0.0	0.0	8.546506337341567	0.0
11	5.199870003249918E-4	0.0	0.0	9.61143971400715	0.0
12	5.199870003249918E-4	0.0	0.0	11.430094247643808	0.0
13	5.199870003249918E-4	0.0	0.0	11.972700682482937	0.0
14	5.199870003249918E-4	0.0	0.0	12.220994475138122	0.0
15	5.199870003249918E-4	0.0	0.0	12.636984075398114	0.0
16	5.199870003249918E-4	0.0	0.0	13.215209619759506	0.0
17	5.199870003249918E-4	0.0	0.0	13.915112122196945	0.0
18	5.199870003249918E-4	0.0	0.0	14.671433214169646	0.0
19	5.199870003249918E-4	0.0	0.0	15.287357816054598	0.0
20	5.199870003249918E-4	0.0	0.0	15.812804679883003	0.0
21	5.199870003249918E-4	0.0	0.0	16.382970425739355	0.0
22	5.199870003249918E-4	0.0	0.0	17.010074748131295	0.0
23	5.199870003249918E-4	0.0	0.0	17.631459213519662	0.0
24	5.199870003249918E-4	0.0	0.0	18.14702632434189	0.0
25	5.199870003249918E-4	0.0	0.0	18.61761455963601	0.0
26	5.199870003249918E-4	0.0	0.0	19.05726356841079	0.0
27	5.199870003249918E-4	0.0	0.0	19.526291842703934	0.0
28	5.199870003249918E-4	0.0	0.0	19.94072148196295	0.0
29	5.199870003249918E-4	0.0	0.0	20.404549886252845	0.0
30	5.199870003249918E-4	0.0	0.0	20.979655508612286	0.0
31	5.199870003249918E-4	0.0	0.0	21.462983425414365	0.0
32	5.199870003249918E-4	0.0	0.0	21.910952226194347	0.0
33	7.799805004874878E-4	0.0	0.0	22.36958076048099	0.0
34	7.799805004874878E-4	0.0	0.0	22.81026974325642	0.0
35	7.799805004874878E-4	0.0	0.0	23.276698082547938	0.0
36	7.799805004874878E-4	0.0	0.0	23.71426714332142	0.0
37	7.799805004874878E-4	0.0	0.0	24.177575560610986	0.0
38	7.799805004874878E-4	0.0	0.0	24.613844653883653	0.0
39	7.799805004874878E-4	0.0	0.0	25.07091322716932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	40	6.7939254E-9	45.0	2
ATCGTAG	20	7.0274266E-4	45.0	1
GGCATCC	35	1.2091186E-7	45.0	8
TCCCCGC	35	1.2091186E-7	45.0	33
TCGACGG	20	7.0274266E-4	45.0	2
GCGTAAG	20	7.0274266E-4	45.0	1
GTCGGTC	35	1.2091186E-7	45.0	21
CGTTTTT	1025	0.0	42.365856	1
CGTAAGG	65	0.0	41.538464	2
CCGATGA	195	0.0	41.53846	18
CATACGA	120	0.0	41.249996	18
TCGTTGA	50	1.0768417E-9	40.5	24
TTAATCG	50	1.0768417E-9	40.5	20
TCGTACG	50	1.0768417E-9	40.5	30
CTCACGA	45	1.9224899E-8	40.0	24
GTACAAG	45	1.9224899E-8	40.0	1
CTCGCCA	45	1.9224899E-8	40.0	42
TACGGCT	410	0.0	39.512196	7
GTTAGCG	40	3.4502227E-7	39.375	1
CGGTCTA	40	3.4502227E-7	39.375	31
>>END_MODULE